Eden S. P. Bromfield

Agriculture and Agri-Food Canada | AAFC · Ottawa Research and Development Centre (ORDC)

Four bacterial strains isolated from root nodules of soybean plants that had been inoculated with root-zone soil of either Amphicarpaea bracteata (Hog Peanut) or Desmodium canadense (Showy Tick Trefoil) growing in Canada, were previously characterized and placed in a novel lineage within the genus Bradyrhizobium. The taxonomic status of the novel s...

The taxonomic status of two previously characterized Bradyrhizobium strains (58S1 T and S23321) isolated from contrasting habitats in Canada and Japan was verified by genomic and phenotypic analyses. Phylogenetic analyses of five and 27 concatenated protein-encoding core gene sequences placed both strains in a highly supported lineage distinct from...

Six bacterial strains isolated from root nodules of soybean plants that had been inoculated with root-zone soil of legumes native to Canada were previously characterized and 1) placed in two novel lineages within the genus Bradyrhizobium and 2) assigned to symbiovar septentrionale. Here we verified the taxonomic status of these strains using genomi...

Four bacterial strains isolated from root nodules of soybean plants that had been inoculated with root-zone soils of legumes native to Canada were previously identified as a novel Bradyrhizobium lineage consisting of symbiovars (sv.) glycinearum and septentrionale. Our purpose was to verify the taxonomic status of these strains using phylogenetic,...

A bacterial strain, designated 39S1MBT, isolated from a root nodule of a soybean plant that had been inoculated with root-zone soil of Amphicarpaea bracteata (hog peanut) growing in Canada, was previously characterized and placed in a novel lineage within the genus Bradyrhizobium. The taxonomic status of strain 39S1MBT was verified by genomic and p...

SUPPLEMENTARY DATA: Strains of Bradyrhizobium barranii sp. nov. associated with legumes native to Canada are symbionts of soybeans and belong to different subspecies (subsp. barranii subsp. nov. and subsp. apii subsp. nov.) and symbiovars (sv. glycinearum and sv. septentrionale). Eden S. P. Bromfield1*, Sylvie Cloutier1, Sawa Wasai-Hara2, 3 and Kiw...

The biology of the weed goat’s-rue, Galega officinalis (Fabaceae), is reviewed. Introduced to Canada in the late 19th Century as an ornamental, it has become established at scattered localities and is spreading locally in southern Ontario. The plant is considered a noxious weed and is legally regulated in many jurisdictions due to the production of...

Supporting Information: Microevolution of symbiotic Bradyrhizobium populations associated with soybeans in east North America

Here, we present the complete genome sequence of the widely studied strain Bradyrhizobium betae PL7HG1 T , isolated from a tumor on the roots of sugar beet. The genome consists of a 7.2-Mbp circular chromosome containing key photosynthesis genes but not genes for nodulation and nitrogen fixation. A putative plasmid was also detected.

The toxic legume plant, Galega officinalis, is native to the Eastern Mediterranean and Black Sea regions. This legume is considered to be a noxious weed, and its establishment in Canada may have resulted from ornamental planting and/or field trials. In its native range, a highly specific nitrogen‐fixing symbiosis with the bacterium, Neorhizobium ga...

We present the complete genome sequence of Bradyrhizobium ottawaense strain OO99 T , a nitrogen-fixing bacterium from root nodules of soybean. The genome consists of a single 8.6-Mb chromosome and includes a symbiosis island. Genes involved in symbiotic nitrogen fixation, stress response, resistance to antibiotics, and toxic compounds were detected...

Bacteria associated with corn roots inoculated with soils collected from the Canadian woodlands were isolated and characterized. Genus-level identification based on 16S rRNA sequence analysis classified the 161 isolates in 19 genera. The majority (64%) of the isolates were affiliated with the genus Pseudomonas. Further analysis of the Pseudomonas i...

An assessment was made of the evolutionary relationships of soybean nodulating bacteria associated with legumes native to eastern Canada to identify potential new sources of soybean inoculant strains. Short season soybeans were used to selectively trap bacteria from root zone soils of four native legume species. Screening of more than 800 bacterial...

The bacterial strain 2-92T isolated from a field plot under long-term (>40 years) mineral fertilization exhibited in vitro antagonistic properties against fungal pathogens. A polyphasic approach was undertaken to verify its taxonomic status. Strain 2-92T is gram-negative, aerobic, non-spore-forming, motile by one or more flagella, and oxidase-, cat...

Two novel Pseudomonas strains (S1E40 and S3E12) isolated from corn roots are antagonistic to Rhizoctonia solani and exhibit differential hypersensitivity reactions on tobacco and corn seedlings. We report here the draft genome sequences of strains S1E40 and S3E12, consisting of 6.98 and 7.06 Mb with 6,150 and 6,129 predicted protein-coding sequence...

Pseudomonas simiae 2-36, isolated from a field plot under long-term mineral fertilization, exhibited strong in vitro antagonistic activities against Rhizoctonia solani and Gaeumannomyces graminis. We report here the draft genome sequence of Pseudomonas simiae 2-36, consisting of 6.4 Mbp with a 60.25% G+C content and 5,790 predicted protein-coding s...

Sixteen strains of symbiotic bacteria from root-nodules of Glycine max grown in Ottawa, Canada were previously characterized and placed in a novel group within the genus Bradyrhizobium. To verify their taxonomic status, these strains were further characterized using a polyphasic approach. All strains possessed identical 16S rRNA gene sequences that...

We report the draft genome sequence of Pantoea ananatis LMG 2665(T), the bacterial causal agent of pineapple fruitlet rot.

Microevolution and origins of Bradyrhizobium populations associated with soybeans at two field sites (A and B, 280 km apart in Canada) with contrasting histories of inoculation was investigated using probabilistic analyses of six core (housekeeping) gene sequences. These analyses supported division of 220 isolates in five lineages corresponding eit...

One hundred and twenty bacterial isolates were obtained from a hypersaline pond (c. 22% salinity) in Sichuan, China. Bacteria were isolated from hypersaline water, sediment and soil samples using three culture media and an incubation temperature of 37°C. Of these isolates, 47 were selected and examined by phylogenetic analysis of 16S rRNA gene sequ...

Variation in nodulation preference within and between cultivars of Medicago sativa for Rhizobium strains was assessed under axenic conditions using inocula consisting of paired strains of R. meliloti which could be recognized by distinctive colony morphology or antibiotic resistance. The largest variability in host preference for Rhizobium strains...

DNA probes for host specific nodulation (hsn) common nodulation (nod), exopolysaccharide (exo), nodule development (ndv) and nitrogenase structural (nif) genes were used to examine a collection of symbiotically effective, genotypically distinct isolates of indigenous Rhizobium meliloti for reiteration of symbiotic DNA sequences on cryptic plasmids....

Isolates of Rhizobium meliloti from indigenous populations at two sites were previously characterized according to phage sensitivity. Isolates representative of the 55 and 65 phage types comprising these two populations, respectively, were tested for denitrification activity with nitrate or nitrite as substrate. Fifty-seven of 120 isolates were cap...

Thirty-three isolates of indigenous Rhizobium meliloti, either possessing cryptic plasmids that hybridize to probes for symbiotic genes or lacking a 1500-kb megaplasmid band in Eckhardt gels, were tested for infectivity on 10 legume species grown under axenic conditions. A previous study had shown that all but two isolates were symbiotically effect...

The insertion sequence ISRm1 frequently occurs in Rhizobium meliloti and is a potential mutagen. Data for the frequency of ISRm1 transposition in the commercial inoculant strain SU47 (containing eight copies of IsRm1) indicate that this insertion sequence does not significantly affect the ability of SU47 to occupy nodules of alfalfa (Medicago sativ...

Indigenous Rhizobium meliloti were previously characterized on the basis of plasmid profiles and phage sensitivity patterns (phage types). Rhizobium meliloti 1076, which contained two cryptic plasmids, was one of four isolates comprising phage type 23. In this study, the large cryptic plasmid pVS1(size >500 b) was transferred from isolate 1076 into...

Populations of indigenous Rhizobium meliloti isolated from nodules of alfalfa grown at sites A and B (soil pH, 7.0 and 6.1, respectively) were previously characterized on the basis of phage sensitivity and divided into 55 and 65 phage types. The available iron content of soil at site B was significantly higher than that at site A. Isolates represen...

Phage-resistant and -susceptible bacteria from nodules of alfalfa and sweet clover, grown at a site without a known history of cultivation, were identified as diverse genotypes of Ensifer, Rhizobium and Phyllobacterium species based on sequence analysis of ribosomal (16S and 23S rRNA) and protein-encoding (atpD and recA) genes, Southern hybridizati...

Intercistronic heterogeneity of the 16S-23S rRNA internal transcribed spacer regions (ITS1) was investigated in 29 strains of fluorescent pseudomonads isolated from subterranean seeds of Amphicarpa bracteata (hog peanut). PCR amplification of the ITS1 region generated one or two products from the strains. Sequence analysis of the amplified fragment...