E. Kvesitadze

• Professor (Full) at Georgian Technical University

Technical characterization and chemical analyses, including total content of biologically active compounds and their antioxidant activities in grape, juice and wine of “Chkhaveri”, the western Georgian endemic variety, have been carried out. Amount of phenolic compounds were determined using Folin–Ciocalteu reagent. Existence of flavonoids, catechi...

The analysis of microscopic fungi collection created at theDurmishidze Institute of Biochemistry and Biotechnology revealed 107 strains assimilating 2,4,6-TNT (2,4,6-trinitrotoluene) belonging to the different fungal genera. The strains have been isolated from the polluted areas adjacent to the military grounds and industrial waste waters. It has b...

Thermophilic micromycetes from the collection of microscopic fungi active strain producers of extracellular cellulases has been selected. Hydrolytic potential of cellulase preparations isolated from the selected strains has been investigated according to hydrolysis of cellulose in agricultural wastes. The wastes have been pretreated biologically (b...

The range of Intracellular Enzymes is rather high, and often organisms synthesize enzymes inside of a cell without developing them outside it, into the liquid culture. In this work, it is shown by us that by adding various salts with Mulberry root extract into the liquid culture, it is possible to achieve the Intracellular Enzyme appearing outside...

Xylanase (hemicellulase) is one of the key enzymes in the creation industrial process of natural biopolymers enzymatic degradation. The importance of this enzyme from the viewpoint of alternative sugar and biofuel production from plant row materials is attracting more and more attention. The objective of the present study was the primary selection...

The work is devoted to the isolation, purification, determination of taxonomical characteristics and application in soil improvement and other biotechnological processes halophilic microscopic fungi strains isolated from saline soils of Eastern Georgia (middle part of South Caucasus), where their existence is maximally supposed. In all soilclimatic...

Phytoremediation exploits plant physiological processes to decontaminate environment or to improve food chain safety by phytostabilisation of toxic elements. These phytotechnologies are based on plants remarkable absorption, transportation and metabolic capabilities that allow to uptake and transform environmental organic pollutants into nontoxic c...

Great interest in basidiomycetes for targeted technological treatment of agro-industrial plant substrates is conditioned by their ability to produce lignocellulosic enzymes. The samples of wood-degrading basidiomycetes were collected from different taxonomic niches of Georgia to study biochemistry and physiology of wood-degrading basidiomycetes. 36...

The ability of agricultural and decorative plants to absorb and detoxify TNT and RDX has been studied. All tested 8 plants, grown hydroponically, were able to absorb these explosives from water solutions: Alfalfa > Soybean > Chickpea> Chikling vetch >Ryegrass > Mung bean> China bean > Maize. Differently from TNT, RDX did not exhibit negative influe...

Influence of octane and benzene on plant cell ultrastructure and enzymes of basic metabolism, such as nitrogen assimilation and energy generation have been studied. Different plants: perennial ryegrass (Lolium perenne) and alfalfa (Medicago sativa); crops- maize (Zea mays L.) and bean (Phaseolus vulgaris); shrubs - privet (Ligustrum sempervirens) a...

The collection of microscopic fungi isolated from different ecological niches of the South Caucasus and accounting of more than 2000 Cultures has been created. As a result of the selection, some strains of fungi including several extremophiles, actively producing xylanase under deep (submerge) conditions of cultivation have been selected. In the co...

The possibility to regulate phenoloxidase activity with sulfur dioxide was studied. It was found that this compound is a potent inhibitor of phenoloxidase of the reversible and mixed type. The inhibitory effect of sulfur dioxide on phenoloxidase provided grounds for a new biotechnological approach to the production of instant green tea. This approa...

Fungi, comprising about 4000 cultures, were collected from different climatic zones of the southern Caucasus. Almost all cultures in the collection showed a high potential for degrading basic plant biopolymers such as cellulose and hemicellulose. Cultures of Penicillium canescens possessing comparatively low cellulase activity in their wild type we...

Genetically stable mutants of Penicillium canescens 20171 with modified cultural and morphological properties were obtained by UV induction. A group of mutants with increased capacity for biosynthesis of extracellular xylanases was isolated and characterized. The P. canescens RTM-22 and XOC-8 mutants were shown to have the highest xylanase activiti...

Allescheria terrestris, Chaetomium thermophile, Aspergillus wentii, and Aspergillus versicolor were selected from the collection of thermophilic microscopic fungi containing more than 50 cultures. The selected cultures displayed the ability to produce thermostable exogenous endoxylanases during submerged cultivation at 40°C. These cultures were sho...

A total of 3966 micromycete cultures were isolated from different soil-climatic zones of Georgia; 20% of them were toxic and 6% were thermophilic. UV irradiation, ethylene imine, and nitrosomethylurea were used to produce mutant strains synthesizing considerable amounts of cellulase. Protoplast fusion of an obligately thermophilic (Allescheria terr...

The thermostability of endo- and exoglucanases of thermophilic mycelial fungi was studied. Increasing the cultivation temperature of a thermophilic culture of Allesheria terrestris from 40 to 48-49°C results in the formation of a new endoglucanase; it is inactivated only by 20% at 65°C for 6 h in the absence of the substrate. A method for purificat...

Fusion of protoplasts from Allescheria terrestris and Aspergillus niger produced a recombinant culture which grew on the media optimal for parent strains and synthesized the enzymes of the parent cultures. The resulting culture inherited the ability to synthesize endoglucanase from A. terrestris and glucose oxidase from A. niger, whereas the intrac...