E. Dilip de Silva

E. Dilip de Silva
University of Colombo · Department of Chemistry

B.Sc. (Colombo), PhD (Hawaii)

About

186
Publications
35,765
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2,914
Citations
Citations since 2017
31 Research Items
1255 Citations
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
Additional affiliations
December 1997 - March 2016
University of Colombo
Position
  • Professor (Full)

Publications

Publications (186)
Article
Plutella xylostella is a destructive cabbage pest responsible for huge economic losses. Heavy and continuous application of synthetic pesticides to control this pest has resulted in numerous environmental issues. Thus, discovering alternative methods to control P. xylostella, with reduced harmful effects has become crucial. In the present study we...
Article
Ethnopharmacological relevance Vernonia zeylanica (L.) Less (Family: Compositae) is a medicinal plant used as external applications for boils, bone fractures, eczema and internally for asthma in traditional medicine in Sri Lanka. Anti-nociceptive, anti-bacterial and anti-proliferative activities have been reported previously. Aim of the study To i...
Patent
The invention relates to a novel method to isolate a natural product, a flavonoid namely inoscavin A from a Sri Lankan mushroom, medicinal preparation thereof to treat rhabdomyosarcoma cancer condition. This compound was isolated from the terrestrial basidiomycete Fulviformes fastuosus for the first time. The isolation method of the inoscavin A com...
Article
Background: Larvae of the diamond back moth (DBM) Plutella xylostella are destructive cabbage pests causing economic losses worldwide. Continuous application of synthetic pesticides to control this pest has resulted in environmental pollution and resistant pest strains. Thus, there is a crucial need to seek natural alternatives with minimal detrim...
Article
Full-text available
Ethnopharmacological relevance: Sri Lanka is known to have very diverse flora. Many of these are used for plant-based remedies, which form the integral part of two Sri Lankan systems of traditional medicine, Ayurveda and Deshiya Chikitsa. Despite their widespread use, only a limited number of studies have probed into the scientific evidence for bio...
Article
Full-text available
Culture feeding experiments with [1-¹³C]-acetate, [2-¹³C]- acetate, and [1,2-¹³C]-acetate have shown that the steroid ring B contraction involved in the biogenesis of the unprecedented carbon skeleton of the antibiotic solanioic acid (1) by the fungus Rhizoctonia solani involves cleavage of the C-5/C-6 bond. The study revealed that 9-epi-solanioic...
Article
Background/ Objective: Vernonia zeylanica (L) less is an endemic plant to Sri Lanka. The present study was designed to isolate potential cytotoxic compound/s from chloroform and ethyl acetate extracts of V. zeylanica by bio-activity guided isolation and to evaluate its anti-proliferative effects in three breast cancer phenotypes (MCF -7, MDA-MB-231...
Conference Paper
Full-text available
Plutella xylostella is a highly destructive cabbage pest responsible for huge economic losses worldwide. Novel bio-friendly alternatives are crucial to replace existing synthetic pesticides to minimize human health impacts while reducing the development of pesticide resistant pest strains. Though sedges are known reservoirs of endophytic fungi thei...
Article
Full-text available
In a study designed to determine the antibacterial potential of endophytic fungi inhabiting plants of Cyperaceae family, 72 morphologically distinct endophytic fungi were isolated from six plants and their antibacterial activities against two Gram positive and three Gram negative pathogenic bacterial species were examined. Two antibacterial metabol...
Article
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Purpose: To screen selected endemic plants grown in Sri Lanka on breast cancer stem cells (bCSCs) for their anti-cancer properties Methods: Breast-CSCs expressing CD24-/CD44 + surface markers were isolated from MDA-MB-231 cells by magnetic-assisted cell sorting method and validated using flow cytometry. A panel of forty extracts from barks and leav...
Article
Full-text available
Macrofungi have been valued as medicinal provisions for humankind and are a rich source of natural anticancer compounds. However, the isolation of such compounds from macrofungi is challenging. This review highlights the importance and challenges that meet during an isolation of anticancer compounds from macrofungi. Moreover, it exhibits the impact...
Article
Full-text available
Natural products originating from marine and plant materials are a rich source of chemical diversity and unique antimicrobials. Using an established in vitro model of HIV-1 latency, we screened 257 pure compounds from a marine natural product library and identified 4 (psammaplin A, aplysiatoxin, debromoaplysiatoxin, and previously-described alotake...
Conference Paper
Full-text available
Plutella xylostella, is a destructive pest that affects cabbage cultivations in Sri Lanka causing economic loss. Continuous application of synthetic insecticides has produced environmental and health hazards while the insects have developed resistance. Thus, there is a crucial need for new environmentally friendly alternatives. Endophytic fungi of...
Chapter
Full-text available
Endophytic fungi are ubiquitous in internal tissues of healthy plants and are known to biosynthesis a remarkable array of secondary metabolites with diverse chemical structures and assist host plants to overcome both abiotic and biotic stress factors in their natural environment. Screening technologies have established these natural products are an...
Conference Paper
Full-text available
Insect repellant activities of sedges may be due to the secondary metabolites produced by the endophytic fungi. Thus, the endophytic fungi of sedges can be a potential alternative source of environmentally friendly pest control agents. Accordingly, the current study was designed to investigate the antifeedant and larvicidal activities of the endoph...
Article
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Porcine pancreatic alpha amylase inhibitory activity A B S T R A C T Three new thiodiketopiperazine derivatives, rostratazine A (1), rostratazine B (2) and rostratazine C (3) along with exserohilone (4) and boydine A (5) were isolated and identified from the laboratory culture of Setosphaeria rostrata, an endophytic fungus obtained from the fresh a...
Article
Treatment of the fungal meroterpenoid dhilirolide A (1) with either sodium azide or perchloric acid results in conversion of the dhilirane carbon skeleton of 1 to the 14,15-dinordhilirane carbon skeleton of the products 5–7, with and without concomitant transfer of an acetyl residue to form a C-9 acetate ester. The discovery of these transformation...
Article
Background: Obesity is considered as one of the risk factors for breast cancer. Leptin has been found to be involved in breast cancer progression. Therefore, novel approaches to antagonize biological effects of leptin are much needed. The objective of this study was to evaluate the protective effects of six dietary compounds (quercetin, curcumin,...
Article
Full-text available
Context: Scyphiphora hydrophyllacea is a shrub mangrove plant of the family Rubiaceae and not yet been studied for anti-hepatocarcinogenic effects. Objectives: We investigated possible in vitro anti-hepatocarcinogenic and antioxidant properties of S. hydrophyllacea. Materials and methods: Dried leaves of S. hydrophyllacea were sequentially ext...
Article
Absence of a drug that kills adult filarial parasites remains the major challenge in eliminating human lymphatic filariasis (LF); the second leading cause of long-term and permanent disability. Thus, the discovery of novel antifilarial natural products with potent adulticidal activity is an urgent need. In the present study, methanol extracts of le...
Article
Full-text available
Polysaccharides of marine algae exhibit different structural characteristics and interesting biological functions. In this study, crude polysaccharides (CP) of eleven Sri Lankan marine algae obtained through hot water extraction and ethanol precipitation were investigated for DPPH, alkyl, and hydroxyl radical scavenging activities using electron sp...
Article
Mangifera zeylanica is a plant endemic to Sri Lanka and its bark has been used in traditional medicine to treat some cancers. This study was aimed to isolate potentially cytotoxic compound/s from the hexane extract of the bark of M. zeylanica by bio-activity guided fractionation. The structure of the isolated compound (1) was elucidated using 1H, 1...
Conference Paper
Full-text available
Invasive use of synthetic insecticides has given rise to numerous environmental problems. Thus, there is a crucial need for the development of environmentally friendly and effective insecticides in agricultural pest control. The main aim of the current study was to investigate the antifeedant and larvicidal activities of the endophytic fungi isolat...
Conference Paper
Full-text available
Majority of pathogenic bacteria causing human infections have developed resistance to the existing antibiotics and thus this has become a public health crisis in the world. Therefore discovering new antibacterial drug leads has become an urgent need today. Isolation of the novel antimicrobial solanioic acid from an endophytic fungus of the medicina...
Article
Full-text available
Background Mushrooms inspired the cuisines of many cultures and conventional medicaments for cancer. However, a substantial number of mushroom species are yet unexplored, possessing an unknown chemical, biological and pharmacological profiles. Fulviformes fastuosus is a terrestrial mushroom, which is commonly found in Sri Lankan woodlands. The curr...
Article
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Sri Lankan sponge-derived fungi are an unexploited resource awaiting exploration for drug leads. Three fungi from the internal tissues of a demosponge collected on the west coast of Sri Lanka were isolated and antimicrobial activities of their crude extracts were evaluated. Aspochalasin B, D, M, asperphena-mate and 4-OMe asperphenamate were isolate...
Patent
Full-text available
The invention relates to antimicrobial properties of mycoleptodiscin B against human pathogenic bacteria Bacillus subtilis (UBC 344), Staphylococcus aureus (ATCC 43300), methicillin resistant Staphylococcus aureus (MRSA, ATCC 33591) and the pathogenic fungus Candida albicans (ATCC 90028). Also relates to method of isolation of mycoleptodiscin B fro...
Article
Human lymphatic filariasis (LF) is mainly caused by filarial parasite Wuchereria bancrofti and is the second leading cause of long term and permanent disability in tropical countries. To date, incapability to eliminate long lived adult parasites by current drugs remains the major challenge in the elimination of LF. Hence, in the current study, the...
Article
Ethno pharmacological relevance Mangifera zeylanica Hook.f. (Anacardiaceae) is a plant endemic to Sri Lanka. Its bark has been used in traditional and Ayurvedic medicine for the treatment of various diseases including some cancers. Aim of the study This study was planned to isolate and identify potentially cytotoxic compounds from the bark of M....
Article
Abstract In this study, in vitro cytotoxic and apoptotic effects of the organic extracts (hexane, chloroform, ethyl acetate and methanol) of Mangifera zeylanica fruit peel and flesh on estrogen receptor positive MCF-7 cancer cells were examined. Chloroform extract of the peel was found to be cytotoxic to cancer cells (IC50 78.46 ± 1.7 µg/mL) while...
Article
Full-text available
Emergence of multidrug-resistant pathogenic microorganisms has prompted a worldwide search for new antibiotics from various sources. Endophytic fungi from unique habitats are considered as potential sources of novel bioactive compounds. Sri Lankan mangrove ecosystem is such a distinctive and unexploited resource for the discovery of structurally di...
Research
Full-text available
Two new lanostane-type triterpenoids, 3R,16R-dihydroxylanosta-7,9(11),24-trien-21-oic acid (1) and 3R,16R,26- trihydroxylanosta-7,9(11),24-trien-21-oic acid (2), along with three known lanostanoids, 16R-hydroxy-3-oxolanosta- 7,9(11),24-trien-21-oic acid (3), 3R-carboxyacetoxy-24-methylen-23-oxolanost-8-en-26-oic acid (4), and 3R-carboxyacetoxy- 24-...
Article
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Twenty distinct endophytic fungi were isolated from the surface-sterilized plant parts of Nymphaea nouchali and were identified using morphological and molecular techniques. At 300 μg/disc concentration, eight of the 20 fungal extracts exhibited antimicrobial activities against Staphylococcus aureus (ATCC 25923) and Bacillus cereus (ATCC 11778) whi...
Article
Full-text available
Endophytic fungi are a promising source of novel biologically active compounds including antimicrobials. Plant endophytic fungi of Sri Lanka, an island with exceptionally high biodiversity and endemism, are a vastly untapped resource. Therefore this study was initiated with the objective of examining the antimicrobial producing potential of the end...
Article
Full-text available
Aims: The present study was planned to evaluate cytotoxic and apoptotic properties of the bark of two common mango varieties (Mangifera indica L.) grown in Sri Lanka [Rata Amba (RA) and Karthakolomban (KA)] in MCF-7 (ER positive breast cancer), MDA-MB-231 (triple negative breast cancer), SKOV-3 (ovarian epithelial cancer) cancer cell lines and norm...
Article
Full-text available
The present study investigated the potential anticancer activity of the bark of Mangifera zeylanica, an endemic plant in Sri Lanka that has been traditionally used for cancer therapy. Cytotoxic and apoptotic effects were investigated in vitro using sulphorodamine assay, acridine orange and ethidium bromide staining, caspase-3 and -7 activity, DNA f...
Article
Full-text available
Purpose: To investigate the anti-proliferative and antioxidant potentials of four different solvent extracts of Phoenix paludosa Roxb leaves. Methods: Four different solvent (hexane, chloroform, ethyl acetate and methanol) leaf extracts of the plant were tested for cytotoxicity against four cancer cells, viz, MCF-7 (oestrogen positive breast cancer...
Conference Paper
Full-text available
Curentelly, there is an increased interest towards investigating bioactive natural products obtained from marine organisms that includes seaweeds as functional food material and for pharmaceutical applications. The coastal waters of Sri Lanka is rich in marine flora biodiversity. Bioactivity screening was carried out for Sri Lankan seaweeds Chondro...
Article
Full-text available
Background: Macrofungi have an established history of use in traditional oriental medicine. Anthracophyllum lateritium is a terrestrial macrofungus found in the dry zone forest reserves in Sri Lanka. Yet there are no scientific reports on bioactive properties of this species. Hence, the current study was aimed at determining the antioxidant potent...
Conference Paper
Full-text available
Macrofungi have been valued as edible and medicinal provisions for humankind. Molecules derived from medicinal mushrooms play a dominant role in the discovery and development of effective drug leads for cancer. The current study was aimed at determining the in vitro antiproliferative activity of a Sri Lankan mushroom, Fulviformes fastuosus against...
Article
Full-text available
Abstract: Natural metabolites produced by macrofungi are of great interest as potential antioxidant defensive agents to reduce the oxidative damage caused by free radicals. Primarily, phenolic and flavonoid type metabolites have gained major importance due to the strong capacity of scavenging free radicals. The study was mainly focused to investiga...
Article
Full-text available
Natural metabolites produced by macrofungi are of great interest as potential antioxidant defensive agents to reduce the oxidative damage caused by free radicals. Primarily, phenolic and flavonoid type metabolites have gained major importance due to the strong capacity of scavenging free radicals. The study was mainly focused to investigate the nat...
Article
Full-text available
Opuntia dillenii is an invasive plant well established in the harsh South-Eastern arid zone of Sri Lanka. Evidence suggests it is likely that the endophytic fungal populations of O. dillenii assist the host in overcoming biotic and abiotic stress by producing biologically active metabolites. With this in mind there is potential to discover novel na...
Conference Paper
Full-text available
Sri Lanka is a unique island which possesses high degree of biodiversity. There are about 3,154 indigenous flowering plant species of flora and about quarter (894) of that is endemic to the country. Natural products compounds have recently gained popularity and scientific interest. Researchers have been interested in bioactive compounds from plant...
Article
Full-text available
Solanioic acid (1), a degraded and rearranged steroid that exhibits in vitro antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), has been isolated from laboratory cultures of the fungus Rhizoctonia solani obtained from tubers of the plant Cyperus rotundus collected in Sri Lanka. The structure of solanioic acid (1) was...
Conference Paper
Full-text available
Bioactive compounds isolated from macrofungi play a vital role in the discovery of drug leads for cancer therapy. The current study was aimed at determining the in vitro cytotoxicity of terrestrial basidiomycete, Phellinus repandus against human sarcoma cells (RD cell line). Fruiting bodies of P. repandus were subjected to sequential extraction wit...