Dyah Iswantini

Dyah Iswantini
Bogor Agricultural University | IPB · Department of Chemistry

Doctor of chemistry

About

62
Publications
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249
Citations

Publications

Publications (62)
Article
Full-text available
Continuous inflammation can cause new and more severe diseases, thus effective treatments are needed. One of the common inflammation treatments is given by reducing prostaglandins' production through the inhibition of COX-2 activity. This experiment aims to examine the potential application of plant extracts of Adenostemma lavenia and Muntingia cal...
Article
Adenostemmoic acid B (AB) is a major compound found in Adenostemma lavenia; it shows anti-melanogenic, anti-inflammatory, and cytotoxic activities. By modifying the 19th position (carboxy: involved in the avoidance of cytotoxicity) of AB, we succeed to separate these activities. Short-chain alkylation of the carboxy group enhanced anti-melanogenic...
Article
This study aimed to evaluate the potential of marine fungus Purpureocillium lilacinum isolated from an Indonesian marine sponge Stylissa sp. as an anti-obesity agent through pancreatic lipase inhibition assay. The fungus was identified as P. lilacinum through morphological and molecular characteristics. The fungal extract’s inhibition activity and...
Article
Full-text available
Five polyaromatic hydrocarbons (PAHs) degrading bacterial species had been isolated from crude oil samples. All bacteria were positive Gram-stained, except one; and had positive results on the catalase test. After sequencing bacterial DNA, three bacterial genera were obtained with 99-100% certainty, namely: Pseudomonas sp., Staphylococcus sp., and...
Article
In this study, angiotensin-I-converting enzyme inhibitory (ACEI) activity was evaluated in fermented goat milk fermented by lactic acid bacteria (LAB) from fermented foods and breast milk. Furthermore, the potential for ACEI peptides was identified in fermented goat milk with the highest ACEI activity. The proteolytic specificity of LAB was also ev...
Article
Monitoring of water quality from the presence of polyaromatic hydrocarbon (PAHs) compounds and its derivates are important for keeping the healthy aquatic environment. Some of those derivates are phenol and several related compounds sharing simmilar structures. This reseach aimed for the detection of those phenol and several similar compounds monit...
Article
Adenostemma lavenia (L.) Kuntze (Asteraceae) is widely distributed in tropical regions of East Asia, and both A. lavenia and A. madurense (DC) are distributed in Japan. In China and Taiwan, A. lavenia is used as a folk medicine for treating lung congestion, pneumonia, and hepatitis. However, neither phylogenic nor biochemical analysis of this plant...
Article
A new method of phenol measurement based on cyclic voltammetry has been tried using Pseudomonas fluorescens biofilm as a bioreceptor. The research is aimed at developing a new measurement for detecting phenol and related compounds using bacterial biofilm. Therefore, it can be further developed for in situ phenol and related compounds monitoring. Th...
Article
Rubak YT, Nuraida L, Iswantini D, Prangdimurti E, Sanam MUE. 2021. Peptide profiling of goat milk fermented by Lactobacillus delbrueckii ssp. delbrueckii BD7: Identification of potential biological activity. Biodiversitas 22: 3136-3145. This study investigated the angiotensin-converting enzyme (ACE) inhibitory activity in fermented goat milk by Lac...
Article
Galangal is widely cultivated for the multifunctional plant. This study analyzed the essential oil chemical components, metabolite profiles, and idioblast cell densities in seven galangal accession grown at two different altitudes (locations A and B). The galangal accessions included: Purbalingga, Cilacap, Purworejo, Karanganyar, Pacitan, Madiun, a...
Article
Full-text available
Fruit extract of asamgelugur ( Garciniaatroviridis ) can be used as an anti-obesity by in vitro against pancreatic lipase activity. The pancreatic lipase plays a role in degradation of lipids into fatty acids, as pancreatic lipase activity is hampered, the absorption of monoglycerides in the human body decreases. The purpose of the research was to...
Article
The modification of Takari natural sand‑based silica with bovine serum albumin/BSA (SiO2@BSA) as an adsorbent for biogenic amines compounds has been successfully synthesized. The SiO2@BSA was synthesized by using the batch method, then was characterized by using FTIR and SEM. Here, A typical BSA group was identified with the new formed namely C–N a...
Article
Full-text available
Electrochemical biosensor based on Bacillus licheniformis whole cell can detect urea level in a synthetic urine. We employed a synthetic urine solution as the urea source, and the level was indirectly monitored through the ammonia oxidation reaction using B. licheniformis microorganism on an electrode surface. The working electrode was a screen-pri...
Article
Full-text available
An amperometric biosensor for 1,2-dihydroxynaphthalene (DHN) and catechol (Cat) has been developed in order to monitor the biodegradaton of polycyclic aromatic hydrocarbons (PAHs). DHN is a common intermediary metabolite in naphthalene and phenanthrene degradation, while Cat is produced by further degradation. These compounds were detected by a bio...
Conference Paper
Biosensor is an analytical device which integrates transducer with bio-component. This analytical device, then, is able to alter a biological signal into measured signal. This ethanol biosensor has Acetobacter aceti biofilm based on screen printed carbon electrode (SPCE) and ferrocene as mediator. Ethanol is determined by electrochemical method usi...
Conference Paper
Full-text available
Hyperuricemia is a condition characterized by levels of blood's uric acid that is higher than its normal levels. If left unchecked, hyperurecemia will trigger gout, an accumulation of mononosodium urate crystals caused by a disruption of purine metabolism in the joints, periarticular tissues, bones and other organs. Xantine oxidase (XO) is an enzym...
Article
Galanga (Kaempferia galanga L.) is one of the potential medicinal plants with high demand.Therefore,galanga cultivation was still quite promising. To obtain the optimum growth and high rhizome production, the superior galanga accessionsare required. The optimal growth and high production ofgalanga accessions will be obtained if cultivated at the ar...
Article
Full-text available
An eco-friendly phenol biosensor from Bacillus biofilm was prepared and investigated. The biofilm, which produced tyrosinase enzyme, was successfully immobilized on a screen-printed carbon electrode surface. A total of 72 Bacillus isolates were utilized because of their capability to produce tyrosinase enzyme in tyrosine media. Among them, Bacillus...
Article
Full-text available
Background and aim: Fermented milk can be used to produce antihypertensive peptides. Lactic acid bacteria (LAB) with its proteolytic system hydrolyze milk protein during fermentation to produce several peptides, which include antihypertensive bioactive peptides. This study aimed to investigate the ability of indigenous LAB for the production of an...
Article
Most of the alcohol analytical methods are robust and instrumentally expensive. An alternative of ethanol biosensor based on selected biofilm forming Acetobacter aceti bacteria producing alcohol oxidase was constructed on a screen-printed carbon electrode. The enzyme specifically oxidizes the ethanol and generate electrical current that then electr...
Article
Full-text available
Common techniques for measuring the capacity and activity of antioxidants are spectrophotometry, fluorescent, liquid and gas chromathography. The antioxidant measurement using spectrophotometry method has its limitation in sample preparation. Therefore, an appropriate method is needed to measure the antioxidant properties in various types of sample...
Article
Full-text available
Biosensor based on biofilm of L. plantarum has been successfully done for determination of uric acid in human urine compared with colorimetric enzymatic produced relative error of less than 5%. L. plantarum has uricase activity to react with uric acid, to maintain the stability of bacteria forming themselves into biofilms. Magnetite is known to inc...
Article
Full-text available
Cu is one of the few metals that predominate in water. In addition, Cu potentially pollutes to any aquatic organisms due to bioaccumulation and biomagnification. One of the estuaries that experienced pressure due to the presence of Cu is Jagir River estuary in Surabaya. The objective of this study was to determine Cu metal concentration in the wate...
Article
Full-text available
Alcohol is commonly found in food and beverage. This alcohol contents must be precisely measured to ensure the halalness of a product, considering the fatwa of Ulema's Council of Indonesia. Alcohol measurement can be carried out using alcohol biosensor based on microbe from pineapple juice, Saccharomyces cerevisiae yeast, which is known to produce...
Article
Full-text available
Antioxidant biosensor based on D. radiodurans biofilm has been investigated in this research. The biofilm producing SOD enzymes were immobilized on SPCE surface. Optimization of experimental measurements were carried out by the response surface method. The optimum value obtained was at the buffer pH 7, suspension pH 6, and optical density (OD) 0.5....
Article
Determination of uric acid is crucial in the uric acid related diseases management. Uric acid biosensor has been developed as an alternative for conventional spectrophotometric methods. A bacteria of Lactobacillus plantarum Mar8 producing uricase was immobilised on zeolite/K-carrageenan membrane. Then, the system was optimised and both kinetics and...
Article
Full-text available
Mangrove ecosystem has multiple important ecological roles, like connecting nutrient flow from terrestrial ecosystem to marine ecosystem, nurturing place for several marine animals, home to many terrestrial animals, and prevention of erosion and controlling sedimentation process. Moreover, mangrove has many economic and social benefits for the comm...
Article
Full-text available
Fitri L, Meryandini A, Iswantini D, Lestari Y. 2017. Diversity of endophytic actinobacteria isolated from medicinal plants and their potency as pancreatic lipase inhibitor. Biodiversitas 18: 857-863. Endophytic actinobacteria from medicinal plants have high diversity and potency to produce secondary metabolites with various biological functions, in...
Article
Full-text available
Estuary of the River Jagir is a gathering place of various pollutants from human activity in Surabaya. To reduce the various pollutants in the area, it can use phytoremediation technique with mangrove ecosystem. Nowadays, many mangroves can be utilized as phytoremediation agents; however its utilization is still limited on the tree-type which needs...
Article
Full-text available
p>Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to ass...
Article
Full-text available
Lactobacillus plantarum has been known be able to produce uricase for uric acid biosensor. Durability and stability of L. plantarum in generating uricase enzyme was low. Hence, we tried to enhance its durability and stability by immobilizing it onto activated 250 mg zeolite at room temperature using 100 μL L.plantarum suspension and 2.87 mM uric ac...
Article
Full-text available
Alcohol dehydrogenase (ADH) was applied to produce alcohol biosensor. The enzyme was collected from cultured Bacillus sp. in solid media. From 6 tested isolates, bacteria from fermented rice grain (TST.A) showed the highest oxidation current which was further applied as the bioreceptor. Various ethanol concentrations was measured based on the incre...
Preprint
Full-text available
Determining halal product of fermented foods and bevearages becomes important for muslim consumers due to religious codification of halal. In order to help on site analysis, an alcohol biosensor was under development. The biosensor was constructed using bacteria producing alcohol oxidase (AOX). Bacteria from apple juice was isolated in advance to o...
Article
Full-text available
Research of uric acid biosensor used a Lactobacillus plantarum was successfully conducted. Lactobacillus plantarum could produce uricase that could be used as uric acid biosensor. Therefore, lifetime of bacteria were quite short that caused the bacteria could not detect uric acid for a long time. To avoid this problem, development of biofilm for ur...
Book
Tanaman Kamadrah (Croton tiglium L) merupakan tanaman multiguna, karena hampir semua bagian tanamannya dapat digunakan sesuai dengan peruntukaanya. Bahkan tanaman ini apabila dieksplorasi dengan baik tidak menutup kemungkinan akan dapat meningkatkan nilai tambah dari produk akhir yang dihasilkan. Akhir akhir ini dengan meningkatnya pemanfaatan tana...
Article
A glucose biosensor was developed to measure blood glucose levels. The performance of these new glucose biosensors was continuously improved, producing better performance and stability. This research developed a glucose biosensor based on immobilizing glucose dehydrogenase dependent flavin adenine dinucleotides on natural zeolite nanoparticles with...
Article
Full-text available
Sida rhombifolia L. is a traditional medicinal plant that has been known with potential as antigout. The previous research suggested that flavonoids crude extract of S. rhombifolia had an inhibitory activity toward xanthine oxidase by 71% and a spectrophotometric measurement showed that the type of flavonoids crude extract inhibition was a competit...
Article
Determination of uric acid concentration in human urine and blood is needed to diagnose several diseases, especially the occurrence of kidney disease in gout patients. Therefore, it is needed to develop a simple and inexpensive method for uric acid detection. The purpose of the research was to observe the use of Indonesian microbe that was immobili...
Article
Full-text available
The objective of this study was to determine the inhibition effect of celery extracts toward xanthine oxidase by in vitro method, and its active compounds. Roots and herb of celery were extracted using water and ethanol solvents. Results indicated that the herbal ethanol extract had the highest inhibition effect (91.40%) at 1400 ppm. The components...
Article
Asam gelugur fruits of Garcinia often used to reduce body weight. Lengkuas and kencur are traditional herbal that potential for antiobesity because they could reduce the level of phospholipids, triglycerides, and cholesterol. The aim of the research was to evaluate the potencies of these herbal as antiobesity by measurement of their water and ethan...
Article
Apium graveolens, one of the traditional medicinal plants, has a potential as anti-gout. We have reported that flavonoid oiA. graveolens could inhibit activity of xanthine oxidase enzyme up to 85.44%. The aim of the research was to investigate the type of inhibition kinetic of A graveolens ethanol crude extract and its fraction inhibition kinetic,...
Article
Full-text available
Zingiber cassumunar Roxb. (Bangle), Guazuma ulmifolia Lamk. (Jati belanda), and Murraya paniculata (Kemuning) have been used as slimming agents in jamu. A few researches have performed studies on their potency as antiobesity. The aim of this research was to investigate the potency of Z. cassumunar rhizome, G. ulmifolia, and M. paniculata leaf extra...
Article
Full-text available
Microbial glucose sensors have been developed using Escherichia coli bacterial strains from Japan. However, there is interest in developing local bacteria as glucose sensors in Indonesia. In this research, the stability and the potential of a selected number of Indonesian bacteria as glucose biosensors was explored. Results of this study indicate t...
Article
Full-text available
p>ABSTRAK Indonesia terkenal kaya akan keanekaragaman hayati, termasukjenis tumbuhan yang mengandung bahan aktif insektisida. Tanamankamandrah (Croton tiglium L.) merupakan salah satu tanaman obat yangbanyak terdapat di wilayah Indonesia dan telah dimanfaatkan sebagaiinsektisida nabati. Penelitian ini bertujuan untuk mengetahui karakteristikfisiko...
Article
Sida rhombifolia L., or Sidaguri in Indonesian, is a traditional medicinal plant with potential as a remedy against gout. The earlier research reported that flavonoids crude extract from this plant could in vitro inhibit the activity of Xanthine Oxidase (XO) (xanthine: oxygen oxidoreductase EC 1.2.3.2) up to 55% and could be antigout. It is a need...
Article
Identification and taxonomy analysis conducted at Herbarium Bogoriense at Research Centre for Biology, Indonesian Institute of Sciences Bogor. The name of the plant was C. tiglium L. The result of analysis on C. tiglium, ethanol extract as laxative material using the intestinal transit method showed treatment group that received dosage 0.06 mL/30 g...
Article
Full-text available
The result of phytochemical analysis showed that the hexane-soluble extract of seeds of C. tiglium contained fatty acids, terpenoids and alkaloids, while the ethanol-soluble extract of the seeds contained alkaloids, flavonoids, steroids, terpenoids and saponins. Moisture and proximate analysis showed that the seeds contained fat up to 40,1%, protei...
Article
Full-text available
The use of palm kernel cake (PKC) and palm oil mill effluent (POME), substances from palm oil factory wastes, for monogastric is limited by their high cellulose and mannan contents. Hydrolytic enzymes have been supplemented to increase the nutrient digestibility. The maximal digestibility was obtained in the synergistic action of all enzyme compone...
Article
Full-text available
Escherichia coli has a membrane-bound dye-linked quinoprotein glucose dehydrogenase (mGDH) in the form of an apoenzyme. An electrochemical method using an E. coli cell-modified electrode allows to study the kinetics and thermodynamics of the activation of apo-mGDH in vivo with the externally added cofactor of the enzyme, pyroloquinoline quinone (PQ...
Article
Full-text available
Apo-glucose dehydrogenase existing in Escherichia coli is converted to the holoenzyme with exogenous pyrroloquinoline quinone (PQQ) and Mg(2+). Catalytic behaviour of the E. coli cells with the holoenzyme is characterized by a Michaelis-Menten-type equation with a catalytic constant of the cell and apparent Michaelis constants for D-glucose and an...
Article
An electrode modified with immobilized Escherichia coli K12 cells produces a steady-state catalytic current originating from the glucose dehydrogenase activity (GDH) of the bacterial cells. Thus, the catalytic current provides a convenient means of measuring the bacterial GDH activity. E. coli K12 cells cultivated under ordinary conditions exhibit...
Article
Mediated bacterial electrocatalysis is used to follow the activation process of apo-glucose dehydrogenase in Escherichia coli cells. A catalytic current is produced at an electrode modified with the bacterial cells when pyrroloquinoline quinone (PQQ) is added to the test solution containing glucose and an electron acceptor. The current is due to th...

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