Dusan Vucetic

Dusan Vucetic
  • Associate Professor
  • Head of Department at Faculty of Medicine of the Military Medical Academy, University of Defense, Belgrade, Serbia

About

56
Publications
9,258
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226
Citations
Current institution
Faculty of Medicine of the Military Medical Academy, University of Defense, Belgrade, Serbia
Current position
  • Head of Department

Publications

Publications (56)
Article
The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 y...
Article
Flow cytometry (FC) analysis of erythrocyte shape and related biomechanical properties, such as osmotic fragility, have not moved from a research tool to regular clinical testing. The main reason is existing evidence that various pre-analytical factors influence the mathematical interpretation of the data obtained. With an aim to contribute to the...
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Background/Aim. After introduction of carefull selection procedure for blood donors and the implemention of highly sensitive screening tests for transfusion transmissible infections (TTI), blood is very save product concerning TTI. Nevertheless, because of the ?window? period for the pathogen that are testing and the emergence of new pathogens, the...
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OBJECTIVE: Buffy–coat derived platelets (PC–BC) cryopreserved by six different protocols (27 units) were compared with liquid–state stored platelets (85 units). MATERIALS AND METHODS: Cryopreservation was achieved using uncontrolled–rate and original controlled–rate freezing (with compensation of released heat of fusion) procedures in combination w...
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Cell recovery, cryothermal micro–damages and surface antigen expression as predictors for cold–induced GPIbα/CD42b–cluster mediated platelet clearance after controlled–rate vs. uncontrolled–rate cryopreservation Rapid Communication No Abstract
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Despite numerous measures, bacterial sepsis associated with the transfusion remains a major threat. The incidence of septic events induced by platelets transfusion is approximately 10 times higher than with transfused red blood cells due to their storage temperature. This caused new Standard that implements the methods for the detection and reducti...
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Treatment of wounds, especially in patients with systemic diseases, is very difficult and time consuming, and it represents great challenge. Complications like infections and impaired healing are regularly seen in these patients, sometimes leaving almost no other treating option, but amputation surgery. In wound healing process platelets play one o...
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Background: The mass migrations experienced by the Western Balkans in the past decades have significantly changed the demographic structures and have probably altered the prevalence of transfusion-transmitted infections (TTIs) among blood donors. However, data on the prevalence of TTIs in the Western Balkans countries remain incomplete. This study...
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Beside supportive transfusions, hemotherapy involves also performance of apheretic procedures due to removal of accumulated (extreme) quantities and/or replacement of pathologically changed blood constituents (cells and plasma contents, pathogens, metabolic products). Hemo-therapy also involves collection/application of stem cells (SCs) with a goal...
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For detection of blood-borne pathogens in transfusion medicine various serological testing (ST), i.e. Enzyme оr Chemiluminescent Immunoassay (EIA/CIA) and molecular analysis (MA), i.e. Polymerase Chain Reaction or Nucleic Acid Testing (PCR/NAT) are in routine use. PCR/NAT technology is very important for pre-transfusion testing for blood-borne dise...
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Srpska akademija nauka i umetnosti. Sažetak Poremećaj neuromijelitis optica (PNMO) je zajednički sindrom za različite vrste autoimunskih inflamacijskih demijalizirajućih bolesti prvenstveno okrenutih ka optičkom nervu ali i drugim delovima centralnog nervnog sistema. Cilj prikaza je bila analiza postignutih terapijskih efekata TIP u lečenju PNMO, u...
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Background/Aim. Hip fracture is one of the leading causes of death in elderly population. We evaluated the impact of gender differences on mortality rate in elderly patients with hip fracture. Methods. The study included all hospitalized elderly patients (aged over 65 years) with hip fracture (during 2013. year). The patients were classified into f...
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Stem cells (SCs) could be determined as cells able for self-renewal with proliferative capacity and potential to differentiate into all blood cells or some somatic cell types, such as osteo-cytes, chondrocytes, hepatocytes, myocytes, cardiomyocytes and even endothelial cells (plas-ticity or transdifferentiation). Growing clinical use of various cel...
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Background: Early detection of the platelet storage lesion is still a challenge in transfusion practice. Using flow cytometry, we evaluated the appearance of the storage lesion, based on the expression of platelet activation markers, in total platelets and platelet populations. Materials and methods: Buffy-coat-derived platelet concentrates were...
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Bezbednost hemoterapije, kada su u pitanju transfuzijski prenosivi (hematogeno transmisivni) infektivni agensi, značajno je poboljšana revidira-nim/strožim kriterijumima za odabir davalaca (skri-ning) i usavršavanjem testova za detekciju markera (antitela, antigena i/ili genoma) transmisivnih bole-sti. Međutim, potencijalni rizik od prenošenja novo...
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Uvod: Prenošenje Treponeme pallidum putem transfuzije krvi je jedan od najranije prepoznatih infektivnih rizika ovog načina lečenja. Poslednjih godina je povećana incidenca sifilisa u svetu, a skrining testovima se pouzdano dijagnostikuju davaoci koji mogu biti prenosioci ove bolesti. Cilj: Odrediti učestalost i kretanje infekcije sifilisom kod dav...
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Terapijska eritrocitafereza je vrsta terapijske aferezne procedure pri kojoj se otklanja određena količina eritrocita iz cirkulacije bolesnika, najčešće sa ostalim ćelijskim elementima. Prema kategorizaciji indikacija terapijskih afereza (Američko društvo za afereze - ASFA) o korišćenju terapijskih afereza u kliničkoj praksi, terapijska eritrocitaf...
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Background Members of armed forces worldwide are considered to be very susceptible to sexually transmitted infections, thus falling into a high-risk group of blood donors regarding transfusion-transmissible infections. In the Serbian Military Medical Academy a significant number (44% for the period 2005–2013) of blood donations were from members of...
Research
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Background. Members of armed forces worldwide are considered to be very susceptible to sexually transmitted infections, thus falling into a high-risk group of blood donors regarding transfusiontransmissible infections. In the Serbian Military Medical Academy a significant number (44% for the period 2005-2013) of blood donations were from members of...
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SAžetAk Uvod: Groznica Zapadnog Nila je infektivno oboljenje virusne etiologije koje se prenosi na životinje i ljude putem komaraca. Virus se može preneti putem krvi i krvnih produkata kao i transplantacijom solidnih organa. Cilj: Utvrditi prevalencu virusa Zapadnog Nila (VZN) kod dobrovoljnih davalaca krvi u Institutu za transfuziologiju i hemobio...
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Abstract Background. The use of "in-line" filtration of whole blood units aims to reduce mononuclear cell (MNC) count, cytokine production and cell activation in platelet concentrates (PCs). The study goal was to compare non-filtered PC (NF-PC or control group) with "in-line leukodepleted" PC (LD-PC) quality on the basis of residual MNC count and...
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A small dose of the Bordetella pertussis vaccine is used as an adjuvant for the induction of experimental autoimmune encephalomyelitis (EAE) in mice. The effects of two doses of the Pertussis vaccine on clinical signs, antibody titers, and the expression of CD4 and MHC molecules in brain tissue sections of mice with EAE were examined. EAE was induc...
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Institut za transfuziologiju i hemobiologiju VMA, Beograd originalni rad / original paper adekvatnim skladištenjem, značajno je poboljšana sterilnost transfundovanih komponenata. Uprkos ovim merama, bakterijska sepsa povezana sa transfuzijom i dalje predstavlja veliku opasnost i tema je koja se stalno ponavlja u literaturi tokom poslednjih 40 godi-...
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SAŽETAK Uvod: Glavni etiološki faktori TRALI su HLA antitela, anti-HLA-DR, granulo-citna antitela i lipidi koji utiču na oksidazu neutrofila. Prikaz slučaja: Bolesnik starosti 44 godine primljen je radi operacije ugradnje proteze levog kuka. Preoperativni laboratorijski rezultati bili su u granicama normale. Drugog postoperativnog dana, primio je...
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Military personnel is a population group at special risk of exposure to sexually transmitted diseases (STD). In peacetime, STD infection rates among service members are generally 2 to 5 times higher than among civilian population. In time of conflict, the differences can be 50 or more times greater. This study describes sexual behavior as a risk fa...
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Krv se uzima od dobrovoljnih davalaca u sistem međusobno povezanih plastičnih kesa i ovakav zatvoren sistem omogućava dalje procesiranje krvi pod sterilnim uslovima. Centrifugiranje je primarni korak u odvajanju plazme od eritrocita i pripremi ostalih komponenata iz jedinice cele krvi. Kritične promenljive u procesu centrifugiranja su: veličina rot...
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Uvod Kratak uvod u problematiku testiranja davalaca krvi na prisustvo infektivnih agenasa Prenošenje hepatitisa putem transfuzije prvi put je prijavljeno 1943. godine (1, 2). hepatitis koji se prenosi oro-fekalnim putem izazivan je virusom Pregledni članak / Review Paper hepa titisa tipa A (hAV), dok je onaj koji se prenosi krvlju/produktima od krv...
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Ispitivanje činilaca odgovornih za lezije koncentrovanih trombocita dobijenih iz „buffy coat“-a (KT-BC) tokom skladištenja su još uvek predmet velikog interesa. Na osnovu vrednosti ekspresije specifičnih trombocitnih markera (membranskih antigena) na ukupnoj populaciji KT, odrediti stepen njihove aktivacije i/ili oštećenja tokom skladištenja u tečn...
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Human glycoprotein of cartilage (YKL-40) synthesizes chondrocytes and synovial cells in inflammatory conditions or remodels the outer cell matrix in osteoarthritis. The aim of this study was to conduct a parallel analysis between thickness of cartilage and length of osteophytes, ultrasound indicators of joint destruction, with levels of YKL-40 in s...
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Arthrosonography and the Biomarker Cartilage Oligomeric Matrix Protein in the Detection of Knee Osteoarthrosis Effusion Osteoarthrosis of the knee is a degenerative disease with inflammatory episodes objectively shown by arthrosonography. Cartilage Oligomeric Matrix Protein - COMP is a useful biomarker for early cartilage destruction. The aim of th...
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Evaluation of Platelet Activation Parameters as Quality Markers for the Stored Platelets In order to investigate the potential use of platelets activation parameters as routine quality control indicators during liquid storage, 27 PC-BC units were kept at 22 °C for up to 5 days. Routine parameters, including a platelet count, mean platelet volume an...
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Thermodynamical and cryobiological parameters responsible for cell damages during cryopreservation (cryoinjuries) have not yet been completely explained. Thus, freezing procedures should be revised, exactly optimized to obtain an enhanced structural and functional recovery of frozen-thawed cells. The aim of this study was to compare microprocessor-...
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Cryobiologic variables responsible for cell injuries and freezing techniques applicable in medical cryopractice should be revised and/or reengineered for minimizing cryoinjuries and maximizing cell recovery. In this study, the efficacy of different cryopreservation protocols based on platelet (PLT) recovery was evaluated. PLTs (n = 33) were prepare...
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Cryopreservation of platelets is of great interest, since it could extend the shelf life of therapeutic platelet concentrates and facilitate stockpiling and inventory control in blood banking. Despite the use of many cryopreservation procedures the optimal cryopreservation procedure is not defined yet. We have compared the cryopreservation of human...
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The purpose was to evaluate biochemical and functional changes in platelet concentrates prepared from buffy-coat (PC-BC), stored up to five days at temperature of 20 +/- 2 degrees C. Forty-two PC-BC units from random blood donors were examined. In order to determine the biochemical changes we studied the release of lactate dehidrogenase (LDH), chan...
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Purpose: establishing correlation between elevated ALT levels and presence or absence of markers of transfusion transmitted infections. Methods: during a period of four month (Feb-May 1997) 5003 serum samples of random blood donors (ages 18-65) were tested for the presence of HBsAg, anti-HCV, anti-MV and ALT levels. ALT values were established by a...
Article
Infection with hepatitis C virus (HCV) can cause several significant health problems. Although some sensitive and specific assays for detection of antibodies to HCV are available, further refinement of antibody screening, confirmatory assays and standardization of molecular testing are necessary. One of the main obstacles along this way represents...
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To evaluate the presence and prevalence of HBV, HCV, HEV, HDV, HIV and CMV infection markers, serum samples from 206 random blood donors and 142 hemodialysis patients were tested. All sera were tested sequentially by means of commercial IMx test kits for HBsAg, anti-HBc, anti-HBs, HBeAg, anti-HBe, anti-HCV, anti-CMV (Abbott), ELISA test kits for an...

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