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Novel single nucleotide polymorphism loci were identified for teak (Tectona grandis Linn. f.) using a combined Next Generation Sequencing approach on the Illumina MiSeq system with genotyping on the Agena MassARRAY iPLEX™ platform. Three hundred and forty four putative loci were identified through sequencing of DNA fragments following a modified do...
Intra-species variation in specific leaf area (SLA) and leaf area (LA) provides mechanistic insight into the persistence and function of plants, including their likely success under climate change and their suitability for revegetation. We measured SLA and LA in 101 Australian populations of the perennial shrub Dodonaea viscosa (L.) Jacq. subsp. an...
182 SNP markers were developed for Ayous (Triplochiton scleroxylon K. Schum) by incorporating information from two next generation sequencing approaches (RADseq Floragenex and AFLPseq IonTorrent PGM) into a single genotyping panel for MassARRAY® iPLEX™. This set of markers was successfully used to genotype 753 individuals from 43 populations in fiv...
Novel single nucleotide polymorphisms (SNPs) and insertion/deletions (INDELs) were identified for the Bigleaf maple (Acer macrophyllum Pursch) using a combined next generation sequencing approach on the Ion Torrent PGM system with genotyping on the MassARRAY® iPLEX™ platform. Five hundred and ninety-eight putative loci were identified through seque...
A method for extracting plant nucleic acids from lignified plant tissue comprising the steps of: a) exposing the lignified plant tissue to a solution comprising: a cationic surfactant, boric acid and buffer to extract the nucleic acids; b) purifying the nucleic acids extracted in (a)
Climate change is driving shifts in the composition of vegetation but the lack of controls and confounding spatial factors pose challenges for detecting the climate signal in observed changes through time and space. We tested whether climate can be isolated as a driver of spatial vegetation composition at the landscape scale in mediterranean southe...
Atriplex sp. Yeelirrie Station (L. Trotter & A. Douglas LCH 25025) is a highly restricted, potentially new species of saltbush, known from only two sites ~30 km apart in central Western Australia. Knowledge of genetic structure within the species is required to inform conservation strategies as both populations occur within a palaeovalley that cont...