Dulaney Miller

Introduction: Prostate cancer (PCa) ranks as the second most frequently diagnosed cancer and the sixth most prevalent cause of cancer-related mortality in men globally. In this study, we employ a multiomic approach that utilizes both urine cfDNA and extracellular vesicle (EV) RNA-derived analytes to improve the risk stratification of individuals wi...

e17095 Background: Prostate cancer (PCa) ranks as the second most frequently diagnosed cancer and the sixth most prevalent cause of cancer-related mortality in men globally. Here, we employ a multiomic approach that utilizes both urine cfDNA and extracellular vesicle (EV) RNA-derived analytes to improve the risk stratification of individuals with P...

Introduction: Colorectal cancer (CRC) ranks second in cancer morbidity, with approximately 60% of patients being diagnosed at an advanced stage. Here, we apply a multiomic strategy jointly leveraging plasma cell-free DNA (cfDNA) and multiple extracellular vesicle (EV)-derived analytes to enhance CRC diagnostic accuracy. Materials and Methods: Plasm...

Aquatic invasive species are a serious and growing threat to biodiversity. Zebra and quagga mussels ( Dreissena polymorpha and D. rostriformis bugensis ) are freshwater invaders causing substantial ecological and economic damage across Europe and North America. Early detection of invasive mussels and other non‐indigenous species is increasingly nee...

Molecular profiling to characterize the effects of environmental exposures is important from the human health and performance as well as the occupational medicine perspective in space exploration. We have developed a novel exosome-based platform that allows profiling of biological processes in the body from a variety of body fluids. The technology...

This document provides a series of protocols used to extract eDNA or eRNA from marine environmental samples such as small and large volume (filtered) water, sediment or (fine mesh net) plankton. Specifically, these protocols are recommended for use following sampling as described in Mirmin et al. (2021; Environmental DNA sampling protocols for the...

This document provides a series of protocols used to extract eDNA or eRNA from marine environmental samples such as small and large volume (filtered) water, sediment or (fine mesh net) plankton. Specifically, these protocols are recommended for use following sampling as described in Mirmin et al. (2021; Environmental DNA sampling protocols for the...

Understanding the spread and distribution of Non-Indigenous Species (NIS) is key when implementing legislation to maintain good ecosystem health. Environmental DNA (eDNA) has shown great potential to detect aquatic organisms in a rapid and cost-effective way, however their applicability to new environments must be validated prior to their implement...

This protocol is intended to provide guidelines on the curation and establishment of a specimen/tissue bank and associated DNA sequence data to be used as reference material/data for subsequent environmental DNA (eDNA) analysis, with particular emphasis on marine non-indigenous and invasive species.

This document describes a series of protocols for the collection of environmental samples intended for the monitoring and surveillance of marine invasive species by means of eDNA metabarcoding analysis, as described in the associated publication (Fernandez et al. 2021: https://doi.org/10.1016/j.marpolbul.2021.112893).

This document describes a series of protocols for the collection of environmental samples intended for the monitoring and surveillance of marine invasive species by means of eDNA metabarcoding analysis, as described in the associated publication (Fernandez et al. 2021: https://doi.org/10.1016/j.marpolbul.2021.112893).

Invasive Alien Species (IAS) are one of the most contributing constituents of biodiversity loss with the distribution range of an increasing number of IAS being artificially re-shaped by human activities. Rapid and effective tools are therefore needed in order to monitor changes in biodiversity and the dispersal of IAS. Molecular tools such as envi...

Cabled observatories are marine infrastructures equipped with biogeochemical and oceanographic sensors as well as High-Definition video and audio equipment, hence providing unprecedented opportunities to study marine biotic and abiotic components. Additionally, non-invasive monitoring approaches such as environmental DNA (eDNA) metabarcoding have f...

Abstract Environmental DNA analysis has revolutionized the way we study rare, invasive, and endangered taxa. However, if eDNA testing is to become an increasingly reliable tool, high detection sensitivity is crucial. Current eDNA sampling methods, like filtration and precipitation, can only process small volumes of water per sample. If only a few s...