Dulaney MillerExosome Diagnostics · Research and Development
Dulaney Miller
Bachelor of Science
About
10
Publications
763
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71
Citations
Citations since 2017
Introduction
Additional affiliations
June 2021 - August 2023
Exosome Diagnostics
Position
- Advanced Research Associate
Description
- Formulating and executing library preparation and target enrichment molecular workflows for optimal yield, sensitivity, specificity, repeatability, and reproducibility. Whole transcriptome, whole exome, and targeted panel sequencing on NextSeq 500/550/2000 sequencers. Conducting multiomic biomarker discovery efforts. Designing probe bait sets. Presenting findings at conferences and team meetings.
May 2019 - May 2021
Position
- Researcher
Description
- Formulated study designs and analytical pipelines for molecular research in a laboratory setting to enhance traditional approaches to environmental DNA (eDNA) metabarcoding. Developed effective protocols via modifications and supplementation to commercial kits for the isolation of nucleic acids from diverse biological and environmental materials. Trained in library preparation workflow for Illumina high throughput sequencing. Ran complete bioinformatic analyses of from raw NGS Novaseq data.
October 2015 - May 2019
Position
- Researcher
Description
- Carried out complex laboratory technical operations requiring a knowledge of scientific methods in DNA and eDNA extraction, amplification, and data interpretation. Conducted standardized PCR and qPCR testing/analysis procedures, using conventional methods for TaqMan® and KASP™ genotyping assays with Internal Positive Controls. Prepared all associated solutions, reagents, and standards. Managed a PCR-free eDNA laboratory including purchasing, budgeting, training, and coordinating use schedules.
Education
September 2010 - May 2014
Publications
Publications (10)
Molecular profiling to characterize the effects of environmental exposures is important from the human health and performance as well as the occupational medicine perspective in space exploration. We have developed a novel exosome-based platform that allows profiling of biological processes in the body from a variety of body fluids. The technology...
This document provides a series of protocols used to extract eDNA or eRNA from marine environmental samples such as small and large volume (filtered) water, sediment or (fine mesh net) plankton. Specifically, these protocols are recommended for use following sampling as described in Mirmin et al. (2021; Environmental DNA sampling protocols for the...
This document provides a series of protocols used to extract eDNA or eRNA from marine environmental samples such as small and large volume (filtered) water, sediment or (fine mesh net) plankton. Specifically, these protocols are recommended for use following sampling as described in Mirmin et al. (2021; Environmental DNA sampling protocols for the...
Understanding the spread and distribution of Non-Indigenous Species (NIS) is key when implementing legislation to maintain good ecosystem health. Environmental DNA (eDNA) has shown great potential to detect aquatic organisms in a rapid and cost-effective way, however their applicability to new environments must be validated prior to their implement...
This protocol is intended to provide guidelines on the curation and establishment of a specimen/tissue bank and associated DNA sequence data to be used as reference material/data for subsequent environmental DNA (eDNA) analysis, with particular emphasis on marine non-indigenous and invasive species.
This document describes a series of protocols for the collection of environmental samples intended for the monitoring and surveillance of marine invasive species by means of eDNA metabarcoding analysis, as described in the associated publication (Fernandez et al. 2021: https://doi.org/10.1016/j.marpolbul.2021.112893).
This document describes a series of protocols for the collection of environmental samples intended for the monitoring and surveillance of marine invasive species by means of eDNA metabarcoding analysis, as described in the associated publication (Fernandez et al. 2021: https://doi.org/10.1016/j.marpolbul.2021.112893).
Invasive Alien Species (IAS) are one of the most contributing constituents of biodiversity loss with the distribution range of an increasing number of IAS being artificially re-shaped by human activities. Rapid and effective tools are therefore needed in order to monitor changes in biodiversity and the dispersal of IAS. Molecular tools such as envi...
Cabled observatories are marine infrastructures equipped with biogeochemical and oceanographic sensors as well as High-Definition video and audio equipment, hence providing unprecedented opportunities to study marine biotic and abiotic components. Additionally, non-invasive monitoring approaches such as environmental DNA (eDNA) metabarcoding have f...
Abstract Environmental DNA analysis has revolutionized the way we study rare, invasive, and endangered taxa. However, if eDNA testing is to become an increasingly reliable tool, high detection sensitivity is crucial. Current eDNA sampling methods, like filtration and precipitation, can only process small volumes of water per sample. If only a few s...
