Duha Mysire Majeed

Verified

Duha verified their affiliation via an institutional email.

Learn more

Verified

Duha verified their affiliation via an institutional email.

Learn more

• PhD of Science in biotechnology
• Assistant Professor at Nahrain University

Knowledge of genetic basis of stress tolerance could be helpful for a successful breeding program. So, the study was conducted to estimate the molecular and phenotypic performance of several genotype of transgenic rice. The seeds of ten genotypes and two local varieties as control were cultivated for the selection of best genotypes with high econom...

This study was carried out to develop a protocol for callus induction, regeneration, shoot, and root induction in two potato cultivars (Riviera and Almonda) that can be further used to develop genetically modified plants. Callus was induced from leaf and internodal explants on Murashige and Skoog's medium (MS) supplemented with different combinatio...

Background: The anatomy of the stem and leaves are important to separate and identify species. The present work includes comparative anatomical and molecular studies of six taxa belonging to the genus Capsicum L. which are grown widely in Iraq. Anatomical and phylogenetic traits were observed for the identification of different taxa.Methods: For th...

This review provides an overview of the progress made in the field of rice genotypes and DNA markers. Rice is a crucial food source globally, and enhancing its nutritional value and resilience to stresses is of significant interest. The availability of high-quality rice genome sequences and functional genomic resources has accelerated genetic resea...

This study aimed to investigate the stimulation of the formation of phenolic compounds in callus cultures of Origanum vulgare using nanomaterials. Different combinations of plant growth regulators (NAA, BAP, and TDZ) were applied to form the calli from leaf explants. The optimal effect was achieved with a combination of 1 mg/L NAA and 0.5 mg/L BAP,...

Moringa oleifera Lam. tree has often been considered a medicinal and economical plant for its nutritional and medicinal properties. This study is intended to use plant tissue culture and elicitation technologies to increase secondary metabolites in the callus cultures of the M. oleifera. Young nodal segments were cultured on Murashige and Skoog (MS...

The breeding material comprising 17 Iraqi wheat cultivars belongs to three different types, i.e., a) salinity-tolerant cultivars (Dajla, Furat, 1H, 2H, 2N, 3H, 3N, and 7H), b) drought-tolerant cultivars (Sham-6 and Orok), and c) local cultivars (Iraq, Iba99, Iba95, Abu Ghraib-3, Adnanin, Tamoze, and Alrashid) underwent qualitative characteristics a...

The aim of this study was to investigate the genetic diversity and markers associated with salinity tolerance in three genotypes of wheat created for salt tolerance by plant breeding program, as well as two Iraqi varieties using random amplified polymorphic cDNA (RAPD-PCR) with eight primers were used. The results of RAPD marker revealed that there...

Salinity is an abiotic stress factor and a major challenge that has significant negative effects on wheat production. It is also a source of concern for plant breeders leading them to reach reliable screening criteria for salt tolerance in wheat genotypes. The physiological analysis showed that the three salt-tolerant wheat genotypes viz., Dijla, 2...

This study was conducted with the target of determine the role of OsHKT4 and OsHKT6 genes in rice plants under salt stress and observe its gene expression by GUS technology, as well as studying the Na ⁺ and K ⁺ accumulation in different tissues. The results obtained show that OsHKT4::GUS appeared strong GUS activity, expressed mainly in vascular ti...

Verbasceae plants are known for the high diversity of their secondary metabolites. Most of them were used as traditional and healthful effects, therefore the aim of this project focused on enhancing the accumulation of some phytochemical compounds in callus tissue of common mullein (Verbascum thapsus L.) that cultivated under abiotic elicitors (int...

In the present study, the effect of Bay leave ethanolic extract on blood glucose, insulin level, lipid profile , liver and kidney function and body weight in type 2 diabetic rats were investigated. Forty albino male rats(250-300) grams were divided into four groups (10 rats for each), first group treated with distilled water and served as control,...

Verbasceae plants are known for the high diversity of their secondary metabolites. Most of them were used as traditional and healthful effects, therefore the aim of this project focused on enhancing the accumulation of some phytochemical compounds in callus tissue of common mullein (Verbascum thapsus L.) that cultivated under abiotic elicitors (int...

The experiment was carried out at the second research station, College of Agriculture, Al-Muthanna University during the winter season 2018-2019, to investigate the performance of four wheat cultivars namely Rasheed, Iba99, Bhooth and Abu Graib subjected K+ supplants to antagonize Na+ effect on growth, yield and genome of four wheat cultivars. Subs...

The purpose of this study was to isolate the Saccharomyces cerevisiae present on different fruits and performing RAPD and ISSR analyses to know the genetic interrelationship between different S. cerevisiae isolates. Some fruits namely apple, plum, dates, and peach were used as natural sources for S. cerevisiae isolation. The isolated S. cerevisiae...

Three genotypes of wheat developede for saltetoleranceethroughe plant breeding program and two local cultivars were screened for genetic variation under salinity conditions through RAPD and ISSR markers. Eight selected primers (OP1-06, OPE-16, OPN-07, OPO-17, OPD-20, OPL-05, OPI-01 and OPJ-13) were used in randomeamplifiede polymorphic eDNA (RAPD-P...

Salinity stress and low water availability affect crop plants in agricultural systems, limiting the productivity of different crops because the mineral toxicity with deficiency frequently causing high osmotic stress as well as overlap toxic ions with main metabolic processes. The aim of this work was to study (TaGSK1) gene expression in some select...

The aim of this study is detection of salt tolerant gene (TaST) selected wheat genotypes for salt tolerance (1H, 2H , 3H) through plant breeding program as compared with local cultivars (sensitive) under salinity condition. Quantitative reverse transcription-PCR (q-RT-PCR) was used to detect TaST salt tolerant gene in these selected genotypes. Also...

High soil salinity is a major abiotic stress in plant production worldwide. TaNIP gene was identified and cloned through the gene chip expression analysis of a salt-tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription – PCR (Q-RT-PCR) was used to detect TaNIP salt tolerant gene and its expression in some selected wh...

A great mullein (Verbascum thapsus L.) was a famous species in Scrophulariaceae family. It was generally used as herbal medicine. Explants of V. thapsus (leaves and petioles) were cultured in vitro on Murashige and Skoog (MS) medium for shoot proliferation.Plantlet explants were cultured on MS medium supplemented with combination of Benzyl adenine...

The objective of this study is to investigate the effect of the interaction between salinity and plant growth regulators (Kinetin and gibberellin) on the salt tolerance of two bread wheat cultivars (Dijilla) and (Tamooze-2) under salinity conditions. The present study was conducted to assess the effects of Kinetin and gibberellin on plant dry matte...

In previous studies TaSC gene was reported to selectively express in salt tolerance wheat. This work was conducted to reveal the TaSC gene expression in Iraqi selected wheat genotypes (Triticum asetivium) (Dijilla, Furat and N3) and local cultivar (Tamoose) under different salinity levels (0, 10, 16 and 20) ds/m, using real-time polymers chain reac...

In previous studies TaSC gene was reported to selectively express in salt tolerance wheat. This work was conducted to reveal the TaSC gene expression in Iraqi selected wheat genotypes (Triticum asetivium) (Dijilla, Furat and N3) and local cultivar (Tamoose) under different salinity levels (0, 10, 16 and 20) ds/m, using real-time polymers chain reac...

This recent study investigated the differences in the expression of Aflatoxin B1 regulatory gene among clinical and environmental (Aspergillus flavus) isolates. Samples were subjected to RNA isolation, cDNA synthesis and PCR amplification. Electrophoresis demonstrated clean bands 798 bp upon amplification with aflR1 primers. The ability of A. flavu...

Gerbera plant (Gerbera jamesonii) is classified according to the flower colors to four strains: white, yellow, pink and purple. Capitulum and scape explants were tested on MS medium in half or full salts strength, supplemented with different combinations of plant growth regulators cytokinins: kinetin (Kin) and benzeladinine (BA), auxin: indolacitic...

Callus cultures of Hyoscyamus niger L. were initiated from leaf segments cultured on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L Benzyl Adenine (BA) and 0, 1, 2 and 3 mg/L Naphthalene acetic acid ( NAA ). Half of cultures were incubated under light of 16 hr/day, while the other half was incubated under complete darkness. The incubati...

anthers were taken from bean flower buds of the variety Aquadlce and microspore developmental stages were determined according to flower bud sizes. Anthers were cultured on modified Schenk and Hildebrandt medium (SH) supplemented with EDTA ferric monosodium salt to the SH medium which was taken from formulation of Murashige and Skoog (MS), casein h...

he effect of five levels of sodium chloride (0.0, 0.5, 1.0, 1.5, 2) % on callus initiated from immature embryos of three genotypes of Triticum aestivum L. (I.E.Tamose 2, Rabeia, genotype 20) were investigated. Callus fresh and dry weight, cell contents of proline, carbohydrate as well as Na, Cl, K and Ca ions were used as parameters to determine th...

the effect of five levels of sodium chloride (0.0, 0.5, 1.0, 1.5, 2) % on callus initiated from immature embryos of three genotypes of Triticum aestivum L. (I.E.Tamose 2, Rabeia, genotype 20) were investigated. Callus fresh and dry weight, cell contents of proline, carbohydrate as well as Na, Cl, K and Ca ions were used as parameters to determine t...

The genetic development for salt tolerance in wheat is very important approach for the plant breeder to overcome salinity problem. Estimation of salt tolerance by two selected genotypes of wheat (4H, N5) was conducted in plastic house as compared with the local cultivars Tamoz 2 to know the development that happened in salt tolerance in these genot...

Random amplified polymorphic DNA (RAPD) was utilized in order to generate information on polymorphism, genetic relatedness and diversity in two local salt tolerant wheat varieties Dijlah and Furat collected from different locations in Iraq(Latyfia, Twaitha and Hasoa) compared to three local non-salt tolerant varieties Tahdi, Tamose2 and Nakhoa for...

Random amplified polymorphic DNA (RAPD) was utilized in order to generate information on polymorphism, genetic relatedness and diversity in two local salt tolerant wheat varieties Dijlah and Furat collected from different locations in Iraq(Latyfia, Twaitha and Hasoa) compared to three local non-salt tolerant varieties Tahdi, Tamose2 and Nakhoa for...

The effect of root exudates of fifty Sorghum genotypes on seed germination and seedling growth of millet were studied in vitro. The fifty genotypes were divided into four groups according to their effect on percentage reduction in root and shoot and whole plant average lengths of millet seedling growth. The first group caused slight stimulation, th...