Drupad K Trivedi

Drupad K Trivedi
The University of Manchester · Manchester Institute of Biotechnology

BSc, PhD, AFHEA

About

73
Publications
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1,218
Citations
Additional affiliations
January 2020 - present
The University of Manchester
Position
  • Lecturer

Publications

Publications (73)
Article
In Down syndrome (DS) in particular, the precise cellular mechanisms linking genotype to phenotype is not straightforward despite a clear mapping of the genetic cause. Metabolomic profiling might be more revealing in understanding molecular–cellular mechanisms of inborn errors of metabolism/syndromes than genomics alone and also result in new prena...
Article
Breath-based metabolomics (breathomics) is an exciting developing area of biotechnology that centers on the capture, identification, and quantification of volatile organic compound (VOC) patterns in human breath and their utilization as tools in the diagnosis of a broad spectrum of medical problems. With the age of personalized medicines demanding...
Article
In this data-rich age it is no longer necessary to methodically isolate, characterize and measure specific molecules. What is important is to identify which of the hundreds or thousands of resolved and measured 'unknown' molecules are potentially associated with the pathophysiology of interest. We have taken LC-MS data from pregnancy urine and appl...
Article
Metabolomics is currently being adopted as a tool to understand numerous clinical pathologies. It is essential to choose the best combination of techniques in order to optimize the information gained from the biological sample examined. For example, separation by reverse-phase liquid chromatography may be suitable for biological fluids in which lip...
Article
Full-text available
Citation: Trivedi DK, Jones H, Shah A, Iles RK (2012) Development of Zwitterionic Hydrophilic Liquid Chromatography (ZIC®HILIC-MS) Metabolomics Method for Shotgun Analysis of Human Urine. J Chromat Separation Techniq 3:144. Abstract Urine is a product of the body's metabolism and the majority of the metabolic products exiting via the renal system a...
Article
Full-text available
We aimed to investigate the link between serum metabolites, gut bacterial community composition, and clinical variables in Parkinson’s disease (PD) and healthy control subjects (HC). A total of 124 subjects were part of the study (63 PD patients and 61 HC subjects). 139 metabolite features were found to be predictive between the PD and Control grou...
Preprint
Full-text available
Parkinson’s disease (PD) is the second most common neurodegenerative disorder and identification of robust biomarkers to complement clinical diagnosis will accelerate treatment options. Here we demonstrate the use of direct infusion of sebum from skin swabs using paper spray ionisation coupled with ion mobility mass spectrometry (PS-IM-MS) to deter...
Preprint
Parkinson’s disease (PD) is the second most common neurodegenerative disorder and identification of robust biomarkers to complement clinical diagnosis will accelerate treatment options. Here we demonstrate the use of direct infusion of sebum from skin swabs using paper spray ionisation coupled with ion mobility mass spectrometry (PS-IM-MS) to deter...
Preprint
Full-text available
Background The COVID-19 pandemic is likely to represent an ongoing global health issue given the potential for vaccine escape and the low likelihood of eliminating all reservoirs of the disease. Whilst diagnostic testing has progressed at pace, there is an unmet clinical need to develop tests that are prognostic, to triage the high volumes of patie...
Preprint
Full-text available
We aimed to investigate the link between serum metabolites, gut bacterial community composition, and clinical variables in Parkinsons disease (PD) and healthy control subjects (HC). 139 metabolite features were found to be differentially abundant between the PD and Control groups. No associations were found between metabolite features and within-PD...
Article
Full-text available
Parkinson’s disease (PD) is a progressive neurodegenerative disorder, which is characterised by degeneration of distinct neuronal populations, including dopaminergic neurons of the substantia nigra. Here, we use a metabolomics profiling approach to identify changes to lipids in PD observed in sebum, a non-invasively available biofluid. We used liqu...
Article
Full-text available
Background The COVID-19 pandemic has led to an unprecedented demand for testing - for diagnosis and prognosis - as well as for investigation into the impact of the disease on the host metabolism. Sebum sampling has the potential to support both needs by looking at what the virus does to us, rather than looking for the virus itself. Methods In this...
Article
Activated ion mobility measurements provide insights to the stability of tertiary and quaternary structures of proteins and when coupled with dissociation strategies can delineate how the fold is disrupted. In this work, we use 213 nm photodissociation coupled with ion mobility mass spectrometry and collisional activation to probe the conformationa...
Article
Full-text available
Parkinson’s disease (PD) is a progressive neurodegenerative disorder that does not currently have a robust clinical diagnostic test. Nonmotor symptoms such as skin disorders have long since been associated with the disease, and more recently a characteristic odor emanating from the skin of people with Parkinson’s has been identified. Here, dynamic...
Preprint
Full-text available
The COVID-19 pandemic has led to an urgent and unprecedented demand for testing – both for diagnosis and prognosis. Here we explore the potential for using sebum, collected via swabbing of a patient’s skin, as a novel sampling matrix to fulfil these requirements. In this pilot study, sebum samples were collected from 67 hospitalised patients (30 PC...
Preprint
p>Activated ion mobility measurements provide Insights to the stability of tertiary and quaternary structures of proteins and pairing such approaches with fragmentation can delineate which part(s) of the primary sequence are disrupted from a folded structure. In this work we use 213 nm photodissociation coupled with ion mobility mass spectrometry a...
Preprint
Parkinson’s disease (PD) is the second most common neurodegenerative disorder for which identification of robust biomarkers to complement clinical PD diagnosis would accelerate treatment options and help to stratify disease progression. Here we demonstrate the use of paper spray ionisation coupled with ion mobility mass spectrometry (PSI IM-MS) to...
Preprint
Full-text available
p>Parkinson’s disease (PD) is a progressive neurodegenerative disorder that does not currently have a robust clinical diagnostic test. Non-motor symptoms such as skin disorders have long since been associated with the disease and more recently a characteristic odour emanating from the skin of people with Parkinson’s has been identified. Here, dynam...
Preprint
Full-text available
p>A metabolomics profiling approach was conducted to identify diagnostic biomarkers of PD from sebum, a non-invasively available biofluid. In this study, we used liquid chromatography-mass spectrometry (LC-MS) to analyse 274 samples from participants (80 drug naïve PD, 138 medicated PD and 56 well matched control subjects) and detected metabolites...
Chapter
Personalized medicine is a rapidly emerging approach to medicine guided by scientific insights into genetic make-up and molecular dynamics of health and disease. This approach relies on “-omics” technologies that aim to define molecular mechanisms of a human body in health and disease. Personalized medicine is a generic entity that has been practic...
Article
Full-text available
Global ageing poses a substantial economic burden on health and social care costs. Enabling a greater proportion of older people to stay healthy for longer is key to the future sustainability of health, social and economic policy. Frailty and associated decrease in resilience plays a central role in poor health in later life. In this study, we pres...
Article
Full-text available
Parkinson's disease (PD) is a progressive, neurodegenerative disease that presents with significant motor symptoms, for which there is no diagnostic chemical test. We have serendipitously identified a hyperosmic individual, a "Super Smeller" who can detect PD by odor alone, and our early pilot studies have indicated that the odor was present in the...
Preprint
Full-text available
Parkinson’s disease (PD) is a progressive, neurodegenerative disease that presents with significant motor symptoms, for which there is no diagnostic test ( 1–3 ). We have serendipitously identified a hyperosmic individual, a ‘Super Smeller’ that can detect PD by odor alone, and our early pilot studies have indicated that the odor was present in the...
Article
Full-text available
Introduction Mammalian cells like Chinese hamster ovary (CHO) cells are routinely used for production of recombinant therapeutic proteins. Cells require a continuous supply of energy and nutrients to sustain high cell densities whilst expressing high titres of recombinant proteins. Cultured mammalian cells are primarily dependent on glucose and glu...
Article
Full-text available
The metabolism of glycerol-3-phosphate (G3P) is important for environmental stress responses by eukaryotic microalgae. G3P is an essential precursor for glycerolipid synthesis and the accumulation of triacylglycerol (TAG) in response to nutrient starvation. G3P dehydrogenase (GPDH) mediates G3P synthesis but the roles of specific GPDH isoforms are...
Article
Current clinical practices, which focus on a small number of biochemicals directly relevant to the pathophysiology within patients, only describe a very limited metabolome of the patient, and do not generally consider the interactions of these small molecules. This lack of information may prevent clinicians from making the best possible therapeutic...
Article
The application of Raman spectroscopy as a detection method coupled with liquid chromatography (LC) has recently attracted considerable interest, although this has currently been limited to isocratic elution. The combination of LC with rapidly advancing Raman techniques, such as surface-enhanced Raman scattering (SERS), allows for the rapid separat...
Article
There has been an increasing demand for rapid and sensitive techniques for the identification and quantification of pharmaceutical compounds in human biofluids during the last few decades and surface-enhanced Raman scattering (SERS) is one of a number of physicochemical techniques with the potential to meet these demands. In this study we have deve...
Article
Full-text available
Bacillus are aerobic spore-forming bacteria that are known to lead to specific diseases, such as anthrax and food poisoning. This study focuses on the characterization of these bacteria by the detection of lipids extracted from 33 well-characterized strains from the Bacillus and Brevibacillus genera, with the aim to discriminate between the differe...
Article
Full-text available
Efflux pumps are critically important membrane components that play a crucial role in strain tolerance in Pseudomonas putida to antibiotics and aromatic hydrocarbons that result in these toxicants being expelled from the bacteria. Here, the effect of propranolol on P. putida was examined by sudden addition of 0.2, 0.4 and 0.6 mg mL-1 of this β-bloc...
Data
Box-whisker plots of a few selected most significant metabolites between the wild type and the mutants in the absence of propranolol. (A) P. putida DOT-T1E is the wild type, (B) P. putida DOT-T1E-PS28, and (C) P. putida DOT-T1E-18. Variables 9 (unknown), Variables 29 (leucine), Variables 37 (leucine^), Variables 70 (unknown), Variables 134 (à-D-glu...
Data
Schematic metabolic pathway diagram of central carbon metabolism in P. putida DOT-T1E showing the level of metabolites for both mutants compared to the wild type. Metabolites were detected and identified by GC-MS. Metabolites indicated in black were observed, while metabolites indicated in grey were not detected. The median m/z intensity (red line)...
Data
Schematic metabolic diagram of central carbon metabolism in P. putida DOT-T1E-PS28 adapted to propranolol showing the level of metabolites for cells exposed to propranolol compared to the control. Metabolites were detected and identified by GC-MS. Metabolites indicated in black were observed, while metabolites indicated in grey were not detected. T...
Data
Box-whisker plots of the detected metabolites of central carbon metabolism in P. putida DOT-T1E, DOT-T1E-PS28 and DOT-T1E-18. Dashed lines separate different concentration levels of propranolol and solid line separates different strains. The label is constructed in a format of “Aac”, “A” represents strains, varies from A to C: A = P. putida DOT-T1E...
Data
Box-whisker plots of the detected metabolites of central carbon metabolism in P. putida DOT-T1E, DOT-T1E-PS28 and DOT-T1E-18. Dashed lines separate different concentration levels of propranolol and solid line separates different strains. The label is constructed in a format of “Aac”, “A” represents strains, varies from A to C: A = P. putida DOT-T1E...
Data
MB-PCA scores plot of GC-MS data for the wild type and the mutants in the absence of propranolol. Colours represent different strains. (A) P. putida DOT-T1E is the wild type (red), (B) P. putida DOT-T1E-PS28 (green), and (C) P. putida DOT-T1E-18 (blue). (PDF)
Data
MB-PCA loading plot of GC-MS data showing the most significant metabolites in the presence of different concentrations of propranolol. Significant loadings were observed in the positive and negative sides of the plot. (PDF)
Data
The level of metabolites for both mutants compared to the wild type in the central carbon metabolism in P. putida DOT-T1E. Metabolites were detected and identified by GC-MS. (PDF)
Data
Viability of P. putida cells 1 h later after exposure to propranolol. (PDF)
Data
Validated PC-DFA models of (A) P. putida DOT-T1E, (B) P. putida DOT-T1E-PS28, (C) P. putida DOT-T1E-18 upon 0.2, 0.4 and 0.6 mg mL-1 Propranolol shock. Symbols coding: control with no propranolol (circles), cells exposed to 0.2 mg mL-1 propranolol (squares), 0.4 mg mL-1 propranolol (triangles), and 0.6 mg mL-1 propranolol (upside down triangles). O...
Data
MB-PCA score plot of GC-MS data showing the effect of different concentrations on P. putida strains. Colours represent different dosage of propranolol. (D0) exposed to 0 mg/mL propranolol (blue), (D1) exposed to 0.2 mg mL-1 propranolol (green), and (D2) exposed to 0.4 mg mL-1 propranolol (pink). (D3) exposed to 0.6 mg mL-1 propranolol (red). (PDF)
Data
Box-whisker plots of the detected metabolites of central carbon metabolism in P. putida DOT-T1E, DOT-T1E-PS28 and DOT-T1E-18. Dashed lines separate different concentration levels of propranolol and solid line separates different strains. The label is constructed in a format of “Aac”, “A” represents strains, varies from A to C: A = P. putida DOT-T1E...
Data
Box-whisker plots of the detected metabolites of central carbon metabolism in P. putida DOT-T1E, DOT-T1E-PS28 and DOT-T1E-18. Dashed lines separate different concentration levels of propranolol and solid line separates different strains. The label is constructed in a format of “Aac”, “A” represents strains, varies from A to C: A = P. putida DOT-T1E...
Data
Results from the propranolol MIC experiments using P. putida DOT-T1E, DOT-T1E-PS28 and DOT-T1E-18. Culture growth was observed after overnight incubation. (PDF)
Article
Full-text available
Introduction: An exceptionally interesting stress response of Pseudomonas putida strains to toxic substances is the induction of efflux pumps that remove toxic chemical substances from the bacterial cell out to the external environment. To exploit these microorganisms to their full potential a deeper understanding of the interactions between the b...
Article
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has successfully been used for the analysis of high molecular weight compounds, such as proteins and nucleic acids. By contrast, analysis of low molecular weight compounds with this technique has been less successful due to interference from matrix peaks whi...
Article
Full-text available
Background The use of electronic cigarettes (e-cigs) is increasing and there is widespread perception that e-cigs are safe. E-cigs contain harmful chemicals; more research is needed to evaluate the safety of e-cig use. Our aim was to investigate the effects of e-cigs on the inflammatory response of human neutrophils. Methods Neutrophils were expose...
Article
Full-text available
Pseudomonas putida strains can adapt and overcome the activity of toxic organic solvents by the employment of several resistant mechanisms including efflux pumps and modification to lipopolysaccharides (LPS) in their membranes. Divalent cations such as magnesium and calcium play a crucial role in the development of solvent tolerance in bacterial ce...
Article
Full-text available
Adulteration of high quality food products with sub-standard and cheaper grades is a world-wide problem taxing the global economy. Currently, many traditional tests suffer from poor specificity, highly complex outputs and a lack of high throughput processing. Metabolomics has been successfully used as an accurate discriminatory technique in a numbe...
Article
Full-text available
In this study we have employed metabolomics approaches to understand the metabolic effects of producing enhanced green fluorescent protein (eGFP) as a recombinant protein in Escherichia coli cells. This metabolic burden analysis was performed against a number of recombinant expression systems and control strains and included: (i) standard transcrip...
Article
Full-text available
Microalgae produce metabolites that could be useful for applications in food, biofuel or fine chemical production. The identification and development of suitable strains require analytical methods that are accurate and allow rapid screening of strains or cultivation conditions. We demonstrate the use of Fourier transform infrared (FT-IR) spectrosco...
Article
Full-text available
Campylobacter species are one of the main causes of food poisoning worldwide. Despite the availability of established culturing and molecular techniques, due to the fastidious nature of these microorganisms, simultaneous detection and species differentiation still remains challenging. This study focused on the differentiation of eleven Campylobacte...
Article
Full-text available
Whilst undergoing differentiation, Streptomyces produce a large quantity of hydrolytic enzymes and secondary metabolites, and it is this very ability that has focussed increasing interest on the use of these bacteria as hosts for the production of various heterologous proteins. However, within this genus, the exploration and understanding of the me...
Conference Paper
Introduction Pancreatic cancer is a devastating disease with a poor outcome. Current biomarkers fall short in accessibility, sensitivity, specificity and their ability to distinguish malignant from benign conditions. Metabolomics aims to decipher molecular signatures that will distinguish disease from controls, ultimately leading to novel targets f...
Article
Full-text available
Ubiquinone (also known as coenzyme Q) is a ubiquitous lipid-soluble redox cofactor that is an essential component of electron transfer chains. Eleven genes have been implicated in bacterial ubiquinone biosynthesis, including ubiX and ubiD, which are responsible for decarboxylation of the 3-octaprenyl-4-hydroxybenzoate precursor. Despite structural...
Article
Full-text available
The bacterial ubiD and ubiX or the homologous fungal fdc1 and pad1 genes have been implicated in the non-oxidative reversible decarboxylation of aromatic substrates, and play a pivotal role in bacterial ubiquinone (also known as coenzyme Q) biosynthesis or microbial biodegradation of aromatic compounds, respectively. Despite biochemical studies on...