Drago Kuzman

Drago Kuzman
Sandoz | sandoz · Pharmaceutical Development

About

32
Publications
11,788
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538
Citations

Publications

Publications (32)
Article
Full-text available
Biosimilar drug products must have a demonstrated similarity with respect to the reference product’s molecules in order to ensure both the effectiveness of the drug and the patients’ safety. In this paper the fusion framework of a highly sensitive NMR fingerprinting approach for conformational changes and mathematically-based biosimilarity metrics...
Article
Extracellular vesicles (EVs) are membrane vesicles that are produced by cells to be released into their microenvironment. In this study, we present the EV concentration as a new factor for optimization of industrial bioprocess control. The release of EVs depends on many cell properties, including cell activation and stress status, and cell death. T...
Article
By using static and dynamic light scattering (SLS and DLS), we investigate the effect of ion specificity and solution conditions on the solution behavior of monoclonal antibodies (mAbs). The extracted second virial coefficient, a global measure of the strength of protein-protein interactions, shows a complicated, nonmonotonic behavior. It can be co...
Article
Full-text available
Human tumor necrosis factor α (TNF-α) exists in its functional state as a homotrimeric protein and is involved in inflammation processes and immune response of a human organism. Overproduction of TNF-α results in the development of chronic autoimmune diseases that can be successfully treated by inhibitors such as monoclonal antibodies. However, the...
Data
Pathway Studio metabolic network. File containing the representation of S. aureus metabolic network (gpc format). The file can be viewed by Pathway Studio software http://www.ariadnegenomics.com/products/pathway-studio/.
Data
Gene sets used for GSEA. Excel spreadsheet file containing gene sets generated from TIGRFAM ontology that were used to run GSEA.
Data
Summary table for differentially expressed genes. Excel spreadsheet file summarizing the transcriptional data from our study and publicly available transcriptional profiling results from SAMMD.
Article
Full-text available
Staphylococcus aureus is a highly adaptable human pathogen and there is a constant search for effective antibiotics. Fosfomycin is a potent irreversible inhibitor of MurA, an enolpyruvyl transferase that uses phosphoenolpyruvate as substrate. The goal of this study was to identify the pathways and processes primarily affected by fosfomycin at the g...
Article
The poloxamer P-407 hyperlipidemia mouse is a genetically unaltered, nondiet-induced model of atherosclerosis which is useful in studying effects of lipid lowering drugs. While the model is relatively well described on the biochemical level, the knowledge regarding the global gene expression was lacking so far. As expected, the P-407 elevates plasm...
Article
Full-text available
The widely prescribed lipid-lowering statins are considered to be relatively safe drugs. However, the risk of severe myopathy and drug interactions as a consequence of statin therapy provides a challenge for development of novel cholesterol-lowering agents, targeting enzymes other than HMG-CoA reductase. The novel pyridylethanol-(phenylethyl)amine...
Data
Full-text available
List of genes present on the Sterolgene v0 cDNA microarray
Data
Differentially expressed genes in mouse liver after phenobarbital treatment using the same probability of type I error as for Sterolgene array (Affymetrix GeneChip). Differentially expressed genes as detected by Affymetrix GeneChip using the same probability of type I error as for Sterolgene data (α = 0.1). Only genes that are also present in the S...
Data
Full-text available
Differentially expressed genes in mouse liver after cholesterol feeding using the same probability of type I error as for Sterolgene array (Affymetrix GeneChip). Differentially expressed genes as detected by Affymetrix GeneChip using the same probability of type I error as for Sterolgene data (α = 0.1). Only genes that are also present in the Stero...
Data
Primer sequences used in quantitative RT-PCR analyses
Data
Full-text available
Differentially expressed genes in the mouse liver after phenobarbital treatment (Affymetrix GeneChip). Differentially expressed genes in the mouse liver after phenobarbital treatment as detected by the Affymetrix MOE430A GeneChip (α = 0.001, genes in bold: α = 0.00043).
Data
Differentially expressed genes in the mouse liver after TNF-α treatment (Agilent microarray). Differentially expressed genes in the mouse liver after TNF-α treatment as detected by the Agilent 10 K cDNA microarray (G4104A) (α = 0.01, genes in bold: α = 0.001).
Data
Full-text available
Differentially expressed genes in the mouse liver after fasting (Agilent microarray). Differentially expressed genes in the mouse liver after fasting as detected by the Agilent cDNA microarray (G4104A) (α = 0.01, genes in bold: α = 0.001).
Data
Full-text available
Differentially expressed genes in mouse liver after fasting using the same probability of type I error as for Sterolgene array (Agilent microarray). Differentially expressed genes as detected by Agilent 10 K cDNA microarray using the same probability of type I error as for Sterolgene data (α = 0.05). Only genes that are also present in the Sterolta...
Data
Full-text available
Differentially expressed genes in mouse liver after TNF-α treatment using the same probability of type I error as for Sterolgene array (Agilent microarray). Differentially expressed genes as detected by Agilent 10 K cDNA microarray using the same probability of type I error as for Sterolgene data (α = 0.05). Only genes that are also present in the...
Data
Full-text available
Differentially expressed genes in the mouse liver after cholesterol feeding (Affymetrix GeneChip). Differentially expressed genes in the mouse liver after cholesterol feeding as detected by the Affymetrix MOE430A GeneChip (α = 0.001, genes in bold: α = 0.00043).
Article
Full-text available
Cholesterol homeostasis and xenobiotic metabolism are complex biological processes, which are difficult to study with traditional methods. Deciphering complex regulation and response of these two processes to different factors is crucial also for understanding of disease development. Systems biology tools as are microarrays can importantly contribu...
Article
Full-text available
The interaction between disrupted lipid homeostasis and immune response is implicated in the pathogenesis of several diseases, but the molecular bridges between the major players are still a matter of controversy. Our systemic study of the inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) in the livers of mice exposed to 20-h cytokine/f...
Article
The interaction between disrupted lipid homeostasis and immune response is implicated in the pathogenesis of several diseases, but the molecular bridges between the major players are still a matter of controversy. Our systemic study of the inflammatory cytokine tumor necrosis factor alpha (TNF-alpha) in the livers of mice exposed to 20 h-cytokine/f...
Article
Red blood cell (RBC) shape, behaviour and deformability can be consistently accounted for by a model for the elastic properties of the RBC membrane that includes the elasticity of the membrane skeleton in dilation and shear, and the local and nonlocal resistance of the bilayer to bending. The role of the corresponding energy terms in different RBC...
Article
Full-text available
The natural biconcave shape of red blood cells (RBC) may be altered by injury or environmental conditions into a spiculated form (echinocyte). An analysis is presented of the effect of such a transformation on the resistance of RBC to entry into capillary sized cylindrical tubes. The analysis accounts for the elasticity of the membrane skeleton in...
Article
The aim of this study was to investigate whether erythrocyte deformability is higher in otherwise healthy children and adolescents with asymptomatic isolated glomerular microhematuria and no clinical or laboratory signs indicating renal disorder. Erythrocyte deformability in 33 children and adolescents with unexplained asymptomatic isolated glomeru...
Article
Full-text available
The effect of pH on the red blood cell (RBC) deformability, which is a consequence of a change of cell membrane elastic properties is studied experimentally. With the intention to reduce the effects on deformability of cell geometry and cytoplasmic viscosity, we measured the deformability of the cells with the same volume at various pH of cell susp...
Article
Full-text available
A fluorescent fibre probe with high collection efficiency for fluorescence-lifetime measurements is presented. A numerical model for calculating the collection efficiency for different probe designs has been employed. The position and number of fibres, crystal geometry, crystal-fibre gap and the backside crystal reflectivity are used as evaluation...
Conference Paper
Full-text available
The development and performance of a temperature optical fibre sensor is presented in the article. It is based on fluorescence lifetime measurement, exploiting a simple digital signal processing scheme for on-line fluorescence lifetime determination. Experimental implementation of the sensor was preceded by a numerical ray tracing Monte Carlo optim...

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