Dragana Trkulja

Institute of Field and Vegetable Crops · Small Grains Department

Heat stress represents a significant environmental challenge that adversely impacts the growth, physiology, and productivity of wheat. In order to determine the response to high temperatures of the wheat varieties developed mostly in the Pannonian environmental zone, as well as varietal differences, we subjected seven varieties from Serbia, one fro...

Global agricultural productivity and food security are threatened by climate change, the growing world population, and the difficulties posed by the pandemic era. To overcome these challenges and meet food requirements, breeders have applied and implemented different advanced techniques that accelerate plant development and increase crop selection...

With aim to improve characterization and eventually, utilisation of garlic collection from the Institute of Field and Vegetable Crops in Serbia, the genetic diversity of 52 accessions was estimated based on microsatellite markers, agronomic, biochemical and phenotypic traits (UPOV descriptor). The Shannon index H′ with a value of 0.47–0.95 indicate...

One of the major challenges that plant breeders face in the 21st century is food safety for growing human population coupled with extreme climate changes. Accordingly, the most important breeders' goal is to find appropriate methods in response to these challenges in order to create high-yielding varieties resilient to abiotic and biotic stressors....

The goal of this research is to test breeding material of different geographical origin, and material that is adapted to different agro-climatic regions. This research examines how different wheat genotypes behave in the climatic conditions of the Pannonian Plain, where environmental stress occur more and more frequently. Fifteen divergent wheat ge...

Selection of the most suitable plants or germplasm to produce high quantity and quality of food has been ongoing since the domestication of the crops started almost 10,000 years ago. However, recent genome-based tools have opened opportunities that we could only dream about just 10 years ago. From the low-throughput molecular marker methods first e...

The purpose of this research is to examine the variability in yields and yield components of wheat cultivars in Northern Serbia, examine and visualize their groupings and relationships, and determine the correlations between their traits using principal components analysis (PCA). A total of fifteen wheat genotypes (Triticum aestivum L.), developed...

The Pannonian Plain, as the most productive region of the South-East Europe, has a long tradition of agronomical production as well as agronomical research and plant breeding. Many research institutions from agri-food sector of this region have a significant impact on agriculture. Their well-developed and fruitful breeding programmes resulted in pr...

Focusing on the importance of tomato in human nutrition and the problem of its narrowed genetic variability, the aim of the study was to assess morphological and chemical diversity in IFVCNS germplasm collection. Twenty genotypes were analysed for the following morphological and chemical fruit traits: average mass (g), length (cm), diameter (cm), p...

The objective of this study was to evaluate boron tolerance of wheat genotypes using mature embryo culture. The analysis involved 79 recombinant inbred lines of the International Triticeae Mapping Initiative (ITMI) population and three Serbian varieties with known boron (B) tolerance (Pobeda - sensitive S, Balerina-medium tolerant MT, and Nevesinjk...

Due to climate change, multiple heat events are expected to be an additional limiting factor that will adversely affect wheat production. The study aimed to analyze the physiological response to heat stress in four winter wheat cultivars at different physiological stages under greenhouse conditions during 2019. The net photosynthetic rate, stomatal...

Photoperiod response (Ppd) genes are very important for adaptation of wheat to different agro-climatic conditions, but also have influence on crop yield. The aim of this study was to compare agronomic traits of 10 Serbian wheat cultivars and 54 NILs of cv. Paragon with single, double and triple doses of Ppd-1 alleles. The results showed that the NI...

Determination of genetic diversity and population structure of breeding material is an important prerequisite for discovering novel and valuable alleles aimed at crop improvement. This study’s main objective was to characterize genetic diversity and population structure of a collection representing a 40-year long historical period of barley (Hordeu...

Plant height and heading time are important agronomic traits that significantly contribute to the improvement of wheat adaptability and yield. The aim of this study was to evaluate the level of phenotypic variability of these two traits in a collection of wheat species originating from 20 countries, to analyse its molecular diversity based on the m...

Plant genetic resources are a link between agriculture, environment and trade, so their conservation requires cooperation from different sectors. The existing diversity in genetic resources is the foundation in breeding for new challenges or new markets in the future. The number of crop genetic resources in Serbia is obscure because there is no nat...

The objective of this study was to compare grain yield, nitrogen use efficiency (NUE) and stay-green traits in winter barley under low and high nitrogen (N) fertilization treatments. Field trials were conducted during two growing seasons in a southern Pannonian location (Novi Sad, Serbia) with fifteen six-rowed barley cultivars released between 197...

The aim of this study was to analyze traits of leaf appearance and phenological development related to grain yield gain in winter barley cultivars released over the past 50 years. Field trials with 15 six-rowed winter barley cultivars were conducted during two growing seasons. The main leaf appearance and developmental traits were studied. The dura...

The objectives of this study were to investigate: (1) the effects of genotype, environment, and their interactions on the oil content (OC), protein content (PC) and grain yield (GY) of 25 varieties of winter wheat, (2) the correlations among these traits in different environments, and (3) the effects of different climatic variables and their intera...

The permanent need for efficient plant breeding comes from the increment of human population, which is projected to reach 9.7 million by 2050. Novel approaches could be used to reach these goals more rapidly, raising the question of efficiency, as well. Spike length is one of the important components of grain yield formation in wheat. The influence...

High grain yield potential and good baking properties are the two primary traits that plant producers consider when selecting new winter wheat cultivars (Triticum aestivum L.). NS Igra is a modern winter wheat cultivar developed at the Institute of Field and Vegetable Crops, Novi Sad and released in 2016. Cultivar NS Igra was selected from a cross...

Estimation of the level of durum wheat germplasm genetic diversity is important for its classification and efficient use in breeding programmes. The aim of this study was to assess genetic diversity of durum wheat genotypes developed at the Institute of Field and Vegetable Crops in Novi Sad, Serbia, with 26 morphological characteristics based on th...

Knowledge about the comparative development and grain filling of winter cereals under different environmental conditions is important for stable and high yielding crop production. The objective of this work was to compare patterns of grain filling in bread wheat, barley and triticale grown in the Pannonian region, as well as to investigate relation...

Creation of new higher yield cultivars, adaptation of existing germplasm to a global climate change, increasing resistance to diseases in new genotypes are some of the tasks that breeding have in front of it. The objectives of this research were to assess GE interaction in two different environments across two vegetation seasons and to do associati...

Climate significantly affects cropping systems across Europe. Knowledge of the variability in grain number per unit area and grain weight across different growing seasons and its association with grain yield is important for further improving small grain crop production. The main aim of this study was to compare grain yield and its numerical compon...

Asocijativna analiza je metoda mapiranja lokusa za kvantitativne osobine medu nesrodnim individuama na osnovu velikog broja rekombinacija tokom domestikacije i selekcije gajenih vrsta. Ukoliko se u analizu uključi struktura populacije, koja nastaje usled adaptacije na lokalne uslove sredine ili selekcije, može se značajno smanjiti broj lažno poziti...

Maize (Zea mays L.) displays large genetic diversity created during the history of introduction from its Mexican centre of origin to other parts of the world and adaptation to a range of diverse environments. Despite such diversity, maize breeders use only a small portion of the available maize germplasm to develop modern hybrids. Broadening divers...

Association analysis is a relatively novel approach in quantitative traits studies that allows high resolution mapping and time efficient and direct application on breeding material. Since the markers, which are close to the quantitative trait loci stable across environments and genetic backgrounds, may be valuable for marker assisted selection, we...

2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) is a secondary metabolite in plants that renders defense against phytopatogenic bacteria, fungi, insects and other pest organisms. The biosynthesis of DIMBOA is controlled by nine genes, the first bx1 gene governs the transcription of a key enzyme in DIMBOA biosynthesis. The aim of this study wa...

This study analysed polymorphism of 15 microsatellite loci in the col­lection comprising of 40 genotypes of bread wheat (Triticum aestivum L.), 32 genotypes belonging to other species within Triticum genus and 3 genotypes from Aegilops genus. The results showed significant differences in the variability of the tested loci in bread wheat and related...

The identification of genomic regions that have retained a signature of selection during breeding of soybean (Glycine max (L.) Merrill) may indicate the positions of genes related to important agronomic traits or underlying adaptation to a specific environment. This can be achieved by analysing populations comprised of ancestral and elite varieties...

The microsatellites, as one of the most robust markers for identification of wheat varieties, were used for assessment of genetic diversity and population structure to promote effective use of genetic resources. In this study, the set of 284 wheat varieties were genotyped using 30 microsatellite markers. The chosen SSR markers were located among al...

The objective of this work was to assess the genetic diversity and population structure of wheat genotypes, to detect significant and stable genetic associations, as well as to evaluate the efficiency of statistical models to identify chromosome regions responsible for the expression of spike-related traits. Eight important spike characteristics we...

Microsatellite markers (SSR) were used to study wheat genetic diversity. A set of 87 wheat genotypes was analysed with four SSR markers. Primers used for the amplification of adequate microsatellite loci (Xgwm) are according to RÖDER et al. (2002). Results were obtained using Applied Biosystems 3130 genetic analyser. Total of 28 alleles were determ...

One of the main wheat breeder's goals is determining specific genomic regions which control important agronomical traits. Association analysis is a new strategy with high resolution in plant molecular breeding that could be used to improve the efficiency of marker assisted selection (MAS) for finding important QTLs (quantitative trait loci) or gene...

The aim of this study was to investigate the possibilities of applying molecular markers-microsatellites in breeding boron tolerant wheat. The study comprised the investigation of allelic variability of sixty bread wheat accessions in two microsatellite loci (Xgwm46-7B and Xgwm577-7B) for which was assumed that are placed near the 7B chromosome loc...

Phenotypic assessment of yield and its component traits needs to be performed in replicated trials under multiple environments. Mapping populations such as doubled haploids (DHs) are suitable material for studying marker-trait associations, because they can be evaluated repeatedly in different years or in variable environments, thus having consider...

Doubled haploid (DH) mapping populations are suitable material for genetic analysis of quantitative trait loci (QTL). In order to detect loci associated with heading and flowering time in our agro-climate region, 177 lines of DH population Savana/Renesansa were analysed with five microsatellite markers (GWM18, GWM194, GWM261, PSP3071 and PSP3200)....

Androgenic responses of 10 F1 wheat hybrids and 13 parental genotypes were studied. It was observed that the studied androgenic traits (androgenic capacity, callus yield, and frequency of green plants) were genotype dependent. On average for all genotypes, 10.6% of the anthers were responsive and 12.2 calluses were produced per 100 anthers. Line 9D...

Association analysis is a new approach in wheat genetic studies that enables a more successful implementation of marker assisted selection (MAS) in breeding programs. In this paper, 96 hexaploid wheat genotypes were used for phenotype evaluation during two years, and molecular analysis using four microsatellite markers (Simple Sequence Repeat - SSR...