Dr-Asma Ansari

• Ph. D.
• Assistant Professor at University of Karachi

The purpose of current investigation is to characterize and elucidate the role of Bac‐IB45 as biopreservative to enhance the shelf life of meat at refrigeration. In this study Bac‐IB45 produced by Lactiplantibacillus plantarum KIBGE‐IB45 was characterized for its amino acid analysis and N terminal sequencing. Fourier‐transform infrared spectroscopy...

The utilization of sugarcane bagasse (SB) in fermentation requires pretreatment processes to render fermentable components available to microorganisms. Pretreatment by using ionic liquids (ILs) is considered promising but the high cost is an impediment in its adoption, therefore, a mixture of IL pretreated and untreated SB was utilized to obtain ba...

Gerrmicidal potential of organic fractions of Calotropis procera (Ait.) R. Br. (milkweed) leaves was determined by using agar well dissemination technique. Fractions of milkweed leaves were prepared with ethyl acetate, butanol, water (aqueous) and hexane. Among them, fractions with hexane solvent were proved to be the best germicidal agents. It is...

Nanomaterials have significantly contributed in the field of nanomedicine as this subject matter has combined the usefulness of natural macromolecules with organic and inorganic nanomaterials. In this respect, various types of nanocomposites are increasingly being explored in order to discover an effective approach in controlling high morbidity and...

Emergence of food borne pathogens and multidrug resistant organisms has enforced the screening of natural antimicrobial compounds. Bacteriocins are ribosomally synthesized natural antimicrobial peptides produced by numerous bacteria. These antimicrobial peptides are gaining more attention as alternative therapeutics in pharmaceutical industry as ne...

Background: Pakistan is one of the top five date fruit-producing countries and produced more than 30% wastes in picking, packing, storage, and commercialization stages. The date fruit wastes are usually considered inedible for humans and only used for livestock feed. In current research, Bacillus licheniformis KIBGE-IB3 was screened for pectinase,...

Fructan based biopolymers have been extensively characterized and explored for their potential applications. Linear chained biopolymers, like levan-type fructan, have gained attention because they have exhibited unconventional stretchable and unbendable properties along with biodegradable and biocompatible nature. Current study deals with the chemi...

Background Chitin is a unique structural exopolysaccharide abundantly found in nature. This exopolysaccharide has a unique chemical structure that acts as a protective outermost covering for most of the crustaceans in aquatic ecosystem. This fortification is because of the insoluble nature of this exopolysaccharide which consist of a linear chain o...

In the current research, second most abundant renewable polysaccharide chitin is utilized for meaningful purpose. Owing to auspicious features of chitin it is used as a matrix for immobilization of valuable biocatalyst dextranase. Dextranase belongs to hydrolase family and has broad commercial application in different fields. Functionality of dextr...

Malathion organophosphates considered as the major constituent of herbicides, pesticides and insecticides. Extensively used in agricultural, horticultures and for numerous household applications contributes to precedence organic pollutants leading antagonistic effects on human health and environment. Therefore detoxification of malathion from conta...

Over the last few decades, emerging cases of chemical additives and food borne pathogens have enforced the demand of natural antimicrobial compounds. Bacteriocins are one of the natural alternatives to the chemical food preservatives. The research on new and improved bacteriocins is in full swing due to some limitations of commercially available ba...

Industrial enzymes particularly xylanase have more demand due to its vast commercial application in paper and pulp, food, animal feed industries as well as for bioconversion of lignocellulosic waste into valuable chemical products. Xylanases are hydrolase enzyme that catalyzes the β-1,4 linkages in heteropolysaccharide xylan which ultimately yields...

Chitinase is a glycosyl hydrolase that cleaves chitin into oligomers. Stability of an enzyme during industrial procedures is a challenge because it directly affects the industrial processing of enzyme catalyzed bioproducts. In this study, chitinase was produced by Glutamicibacter uratoxydans. Chitinase was subjected to stability studies in the pres...

To sustain life and promote good health, there is a need to provide sufficient amounts of safe and nutritious food as it is a basic human necessity. In the last few years, the risks associated with the food borne pathogens have greatly increased. The presence of Listeria monocytogenes in fresh and processed foods and listeriosis outbreaks has enfor...

Background The emergence of serious issues of multidrug resistance in the past few years have enforced the use of bacteriocins for combating infections. Threat posed to public health by various multidrug resistant (MDR) organisms can be resolved by discovering new antimicrobial proteins with broad spectrum of inhibition. Results In the current stu...

My presentation is about the combinatorial stretegy used for the enhanced production of bacteriocin. for this first applied mutational analysis afterwards the mutant strain was optimized using statistical tool..

Bacterial maltase catalyzes the hydrolysis of maltose and is known as one of the most significant hydrolases. It has several applications in different industrial processes but widely used in food fermentation technology and alcohol production. In the current study, entrapment technique was comprehensively examined using polyacrylamide gel as a matr...

The bacterial strains capable of producing dextransucrase enzyme were isolated from different fruits and vegetables sources. In primary screening, five strains were selected on the basis dextransucrase production and among them L. mesenteroides KIBGE- IB26 isolated from bottle gourd (Lagenaria Vulgaris) was selected for further studies. For the enh...

Production of antimicrobial compounds is considered as ubiquitous anti-competitor strategy in bacterial ecosystem. Bacteriocins are heterogeneous; highly specific and efficient anti-competitor agents and the gene responsible for the production of bacteriocins mostly exist in an autosomal state and associated with plasmids. BAC-IB17 is a broad spect...

Degradation of high molecular weight dextran for obtaining low molecular weight dextran is based on the hydrolysis using chemical and enzymatic methods. Current research study focused on production, purification and characterization of dextranase from a newly isolated strain of Bacillus licheniformis KIBGE-IB25. Dextranase was purified up to 36 fol...

The continuous exploration of new microbial cell factories in diverse environmental and geographical locations has led to pivotal developments in the production of industrially important extracellular metabolites. Filamentous fungi are used for large-scale production of various enzymes, and amylolytic enzymes in particular. Among them, amyloglucosi...

The emergence of serious issues of multidrug resistance in the past few years forced the consideration of bacteriocins for combating infections. Numerous concerns have been raised against increased bacterial resistance toward effective drugs and become a debated issue all over the world. Alongside, there is an increase in consumer demand for the an...

Increased bacterial resistance towards commonly used antibiotics has become a debated issue all over the world in a last few decades. Due to this, consumer demand towards natural anti-microbial agents is increasing day by day. Natural anti-microbial agents have gained enormous attention as an alternative therapeutic agent in pharmaceutical industry...

Xylan degrading bacterial strain was isolated from soil and identified as Geobacillus stearothermophilus KIBGE-IB29 on the basis of morphological, biochemical and 16S rDNA sequence analysis. Optimization of medium and culture conditions in submerged fermentation was investigated for maximum endo-1, 4-β-xylanase production. High yield of xylan degra...

Utilization of highly specific enzymes for various industrial processes and applications has gained huge momentum in the field of white biotechnology. Selection of a strain by efficient plate-screening method for a specific purpose has also favored and boosted the isolation of several industrially feasible microorganisms and screening of a large nu...

a b s t r a c t Dextranase, 6-alpha-d-glucan 6-glucanohydrolase catalyzes the degradation of dextran (polymer of d-glucose) in to low molecular weight fractions. Dextranolytic bacterial strains were isolated from various natural sources and plate assay methods were developed for screening of highest extracellular dextranase producing isolate. Bacil...

Bacteriocins are peptides produced by a variety of different microbes and have antimicrobial activity against closely related species. These antimicrobial agents are gaining more and more attention as an alternative therapeutics not only in pharmaceutical but also as a preservative in food industries. In this study several bacterial strains were is...

The activity of commonly used spices and salt, sugar and pickles against Escherichia coli, Salmonella typhi, and Staphylococcus aureus was tested. The antibacterial activity was found to be in descending order like coriander>pickles>salt and sugar>clove>turmeric>blackpepper> red chilli againstS. typhi and garlic>clove>onion>ginger against S. aureus...