Dora Bojcsuk

Dora Bojcsuk
University of Debrecen · Department of Biochemistry and Molecular Biology

About

19
Publications
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149
Citations

Publications

Publications (19)
Article
Full-text available
Retinoid X receptor (RXR) plays a pivotal role as a transcriptional regulator and serves as an obligatory heterodimerization partner for at least 20 other nuclear receptors (NRs). Given a potentially limiting/sequestered pool of RXR and simultaneous expression of several RXR partners, we hypothesized that NRs compete for binding to RXR and that thi...
Article
Full-text available
Background Preferential accumulation of fat in the upper body (apple shape) is associated with higher risk of developing metabolic syndrome relative to lower body fat (pear shape). We previously discovered that chromatin openness defined partially the transcriptome of pre-adipocytes isolated from abdominal and gluteofemoral fat. However, the molecu...
Article
Full-text available
Super-enhancers (SEs) are clusters of highly active enhancers, regulating cell type-specific and disease-related genes, including oncogenes. The individual regulatory regions within SEs might be simultaneously bound by different transcription factors (TFs) and co-regulators, which together establish a chromatin environment conducting to effective t...
Article
Estrogen Receptor alpha (ERα) is a ligand-activated transcription factor and it has a prominent role in both physiological and pathological processes of the reproductive system. ERα has been investigated extensively in breast cancer and the MCF-7 breast-cancer-derived cell line is a widely used model for the study of its behavior. In this paper we...
Article
Full-text available
Background Increased lower body fat is associated with reduced cardiometabolic risk. The molecular basis for depot-specific differences in gluteofemoral (GF) compared with abdominal (A) subcutaneous adipocyte function is poorly understood. In the current report, we used a combination of Assay for Transposase-Accessible Chromatin followed by sequenc...
Article
Full-text available
There is a growing body of evidence that poly(ADP-ribose) polymerase-2 (PARP2), although originally described as a DNA repair protein, has a widespread role as a metabolic regulator. We show that the ablation of PARP2 induced characteristic changes in the lipidome. The silencing of PARP2 induced the expression of sterol regulatory element-binding p...
Preprint
Full-text available
Super-enhancers (SEs) are clusters of highly active enhancers, regulating cell type-specific and disease-related genes, including oncogenes. The individual regulatory regions within SEs might be simultaneously bound by different transcription factors (TFs) and co-regulators such as P300, BRD4 and Mediator, which together establish a chromatin envir...
Article
Full-text available
Hepatocyte nuclear factor 4 alpha (HNF4α) nuclear receptor is a master regulator of hepatocyte development, nutrient transport and metabolism. HNF4α is regulated both at the transcriptional and post-transcriptional levels by different mechanisms. Several kinases (PKA, PKC, AMPK) were shown to phosphorylate and decrease the activity of HNF4α. Activa...
Data
HNF4α target genes identified in ChIP-seq experiment on vehicle treated HepG2 cells. (XLSX)
Data
P values of one-sample t-test on individual HNF4α target genes investigated by ChIP-qPCR. (XLSX)
Data
Recruitment of HNF4α and H3K27ac at promoters. Histogram shows the average tag density of HNF4α and H3K27ac peaks at those 451 HNF4α binding sites which overlap with the transcription start site (TSS). (TIF)
Data
ChIP-qPCR results showing HNF4α occupancy on seven genomic regions of hepatic HNF4α target genes. Immunoprecipitation of chromatin from HepG2 cells was performed with anti- HNF4α or IgG antibody untreated or treated with EGF for 30 mins or 24 hours. Enrichment was compared to negative control region (Beta globin) for TF occupancy (y axis). HNF4α bi...
Article
Full-text available
Super-enhancers are established through the interactions of several enhancers and a large number of proteins, including transcription factors and co-regulators; however, the formation of these interactions is poorly understood. By re-analysing previously published estrogen receptor alpha (ERα) ChIP-seq data sets derived from the MCF-7 cell line, we...
Article
Nuclear Receptors are ligand-activated transcription factors that translate information about the lipid environment into specific genetic programs, a property that renders them good candidates to be mediators of rapid adaptation changes of a species. Lipid-based morphogens, endocrine hormones, fatty acids and xenobiotics might act through this clas...

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