Dono Indarto

• Head of Department at Sebelas Maret University

Banana peel is a waste that has the potential as an anti-obesity agent due to its nutritional and phytochemical content. This study aims to analyze and compare the content of macronutrients, micronutrients, and phytochemical compounds in raja banana peel extracts. The maceration method was performed during the preparation of extracts by using ethan...

Background. Bile duct injury (BDI) remains a serious complication of hepatobiliary surgery, particularly in laparoscopic cholecystectomy, often leading to strictures, fibrosis, and long-term morbidity. Although traditional repair techniques such as primary suturing and Roux-en-Y hepaticojejunostomy are widely used, they carry risks of anastomotic c...

Insiden obesitas meningkat di seluruh dunia selama beberapa tahun terakhir. Diet dan aktivitas fisik saja pada penderita obesitas kurang memberikan hasil yang optimal, sedangkan penggunaan obat anti obesitas dalam jangka panjang dapat menyebabkan efek samping. Penelitian ini bertujuan untuk menganalisis nutrisi dan kandungan fitokimia kulit buah sa...

Background Pyroptosis, a highly inflammatory form of programmed cell death, plays a key role in diseases such as sepsis, rheumatoid arthritis, Alzheimer’s disease, and COPD. It is driven by inflammasome activation, leading to the release of pro-inflammatory cytokines. Pyridoxine (Vitamin B6), an essential micronutrient with known anti-inflammatory...

Bile duct injuries (BDI) from surgical procedures pose significant clinical challenges, requiring precise interventions for optimal outcomes. This systematic review explores the utilization of grafts in the repair of bile duct injuries, aiming to gain insights from existing literature. Graft-based techniques show promise in improving postoperative...

Eccentric exercise often leads to oxidative stress, inflammation, and muscle damage that impair athletic performance. To counter these adverse effects, clove flower extract (Syzygium aromaticum) offers promising potential as a natural remedy to promote muscle repair with its potent antioxidant and anti-inflammatory properties. The aim of this study...

Increased incidence of laparoscopic cholecystectomy-related bile duct injuries (BDIs), combined with its risk of serious complications and mortality, highlights the need for a more effective repair technique. Although the use of autologous graft in BDI repair has been promoted, the role of autologous parietal peritoneum remains underexplored. The a...

Pancreatic lipase plays an important role in converting triglyceride into long-chain fatty acids and glycerol, then becomes a therapeutic target for obesity treatment. Quercetin and chrysin are able to inhibit pancreatic lipase, which is potentially developed for obesity treatment. This study aimed to identify quercetin and chrysin derived from the...

Lipid metabolism is the process of breaking down triglycerides into fatty acids and glycerol, which are oxidized to provide energy. Peroxisome proliferation-activated receptor (PPAR)- alpha and adenosine monophosphate protein kinase (AMPK) are key proteins to increase endogenous lipolysis, which become important targets for weight loss management....

Obesity is a global health problem that prevalence has increased in the last decade. Triglycerides are the main fat component in daily food intake, which can be absorbed directly into the enterocytes through Cluster of Differentiation (CD) 36 transporter protein. Glucagon-like peptide 1 receptor agonist (semaglutide) has recently been approved for...

Third-hand smoke (THS) refers to the residual toxic substances that settle on surfaces following the combustion of tobacco. Although concerns about its health implications are increasing, studies exploring its impact on the lungs and potential protective strategies remain scarce. Thus, this study explored the potential of pyridoxine (vitamin B6), k...

Iron nutritional anemia is a prevalent health issue among adolescent girls. Several factors contribute to anemia in this demographic, including inappropriate growth for age, poor nutritional intake, unhealthy food habits, inadequate or excessive physical activity, irregular menstrual cycles, and lack of nutrition knowledge. To address this, the gov...

Our previous studies show that Berastagi orange peel extract (BOPE) has anti-obesity properties, indicated by its active compounds and effects on reducing body weight gain and body fat percentage. This present study further analysed the effects of BOPE by evaluating docking analysis of BOPE’s phytochemicals and measuring lipid profiles, atherogenic...

BACKGROUND Stroke is the third leading cause of morbidity and second in mortality worldwide. Diabetes mellitus (DM) is a risk factor for stroke. Vaspin and single nucleotide polymorphism (SNP) rs2236242 involved DM pathogenesis. This study aimed to explore the correlation between SNP rs2236242, serum vaspin levels, and atherosclerosis in patients w...

Mirabilis jalapa merupakan tumbuhan tropis dengan berbagai warna yang banyak ditemukan di Indonesia namun saat ini pemanfaatannya hanya sebatas tanaman hias dan pagar. Kombinasi M. jalapa warna kuning dan pink memiliki fitokimia yang berperan dalam metabolisme zat besi tetapi belum ada penelitian terkait dengan kandungan zat gizi. Penelitian ini be...

Aims: To determine the impact of MSC secretome on the nuclear factor-kappa B (NF-kB) expression in the liver. Methods: This research is a posttest-only control design group experimental study. This experiment used 40 mice. There were eight mice per group, and the animals were randomized, the sepsis mice group, the control group, the sepsis mice gro...

Context: L-citrulline is a non-essential amino acid primarily sourced from watermelon (Citrullus vulgaris). Many studies on L-citrulline have been conducted in the past 10 years. However, no bibliometric analyses have been performed. Aims: To establish bibliometric analysis as a comprehensive review of L-citrulline literature. Methods: Articles on...

Nugraheni MA, Indarto D, Pamungkasari EP, Susilawati TN, Jusup SA, Purwaningtyas N, Rahardjo SS, Wulandari S, Devina KE. 2024. The effects of Jebisa on nutrient intake and hematology profile in adolescent girls with anemia. Nusantara Bioscience 16: 210-218. Anemia is a public health problem that often occurs in adolescent girls in developing countr...

Background Primary dysmenorrhea (PD) is characterized by discomfort with no organic etiology (no pelvic disease), recurring pain, or lower abdominal cramps that start between the first 8–72 h of menstruation. Cupping therapy uses a tool to form a vacuum at certain points on the skin. Objectives We investigated the mechanism of pain relief caused b...

There were 463 million people living with diabetes around the world, and it is expected to increase by 48% in 2045. Dipeptidyl Peptidase 4 (DPP4) inhibitor is asecond-line anti-diabetic drug, that frequently causes weight gain for long term treatment. Alkaloid fraction of L. glutinosa leaves (AFLG) is able to inhibit DPP4 activity in diabetic rats....

Vitiligo is a progressive and multifactorial condition of skin, mucosa, and hair depigmentation. Loss of functional melanocytes leads to the appearance of white macules on the skin, often affecting the lips and genitalia. The impact extends to psychological stress, decreased quality of life, and risk of psychiatric morbidity. Various therapeutic st...

Primary dysmenorrhea (PD) is a prevalent problem in gynecology affecting the quality of life for young women. This medical condition is often responsible for the absence of young women from school or work, increasing the risk of developing hyperemesis gravidarum in the future. Furthermore, PD causes pain due to the activation of the local PGF2α pat...

Objective: In Indonesia, programs amid the widespread were proceeded and reinforced into the Merdeka Belajar program which was actualized more comprehensively through 4 programs counting online get to, instructor preparing, learning materials, and crisis educational programs. In any case, the existing science education in Indonesia has to be change...

Objective: Preeclampsia (PE) is the leading cause of maternal death worldwide and is associated with long-term morbidity in both mothers and newborns. Animal modeling is considered a functional source for understanding PE pathogenesis, diagnostic standards, and therapeutic approaches. Materials and methods: This study aimed to demonstrate and ev...

Snake fruit is an Indonesian original plant cultivated in Turi District, Sleman Regency, Yogyakarta Province. Our previous studies indicated that administrations of flour or ethanol extract of snake fruit seeds (SFS) could increase hemoglobin levels in female rats with anemia. However, anemia rats treated with higher doses of SFS flour and ethanol...

Preeclampsia (PE) is a severe pregnancy disorder posing significant health risks to both the mother and the fetus without available preventive measures or treatments. More specifically, the main pathological feature of early-onset PE (EO-PE) is the incomplete transformation of the spiral artery. Meanwhile, aspirin has proven effective for the treat...

p> Background: Obesity is a risk factor for metabolic syndrome which can increase mortality. Obese sufferers often fail to lose body weight (BW) through non-pharmacological therapy. Obesity synthetic drugs can cause side effects, so natural ingredients are needed as alternative therapies. Objectives: This study aims to determine the effect of raj...

Background A potentially fatal complication of sepsis is septic acute kidney injury. Stem cell therapy is a potential new method of treating sepsis and has been applied to treat some human diseases. Objectives This study investigated the effects of secretome-MSCs on NGAL, CRP, NF-κB, and MMP-9 proteins, and histopathology in mice with septic AKI....

Backgrounds : Traumatic brain injury has high mortality and morbidity. The involvement of neuroinflammatory responses and neuronal damage in traumatic brain injury is the basis for the development of renewable neuroprotective agents, one of which is the amino acid L-Arginine. The amino acid L-Arginine which can be found in Giant Snake Head fish ( C...

Objective: To evaluate the PGF2 profile and its association with symptoms in several models of primary dysmenorrhea. Methodogy: The rats were placed into four groups, each with ten rats: control, primary dysmenorrhea 1 (PD1), primary dysmenorrhea 2 (PD2), and primary dysmenorrhea 3 (PD3). The pain as main dysmenorrhea symptom was assessed by observ...

Background Eccentric exercise may trigger mechanical stress, resulting in muscle damage that may decrease athletic performance. L-citrulline potentially prevents skeletal muscle damage after acute eccentric exercise. This study aimed to assess the dose-response effect of L-citrulline as a preventive therapy for skeletal muscle damage in mice after...

Background: Butterfly pea flowers (Clitoria ternatea L.) are edible plants found in Asian countries that have several phytochemical compounds thathave potential anti-obesity and anti-dyslipidemia. From several in vivo studies, the administration of various extracts of butterfly pea flowers has different effects to reduce body weight (BW) or body ma...

Batubara WRP, Susilawati TN, Indarto D. 2023. The potency of Berastagi sweet orange (Citrus sinensis) peel extract for obesity treatment: A study in the rats. Nusantara Bioscience 15: 251-257. Obesity is an abnormal accumulation of body fat and is a trigger factor for various degenerative diseases. Obese continues to grow every year in the world. T...

Background The prevalence of anemia in female adolescents increases every year. A duodenal Cytochrome B ( CYBRD1) enzyme is involved in the regulation of iron metabolism. G797A gene polymorphism of the CYBRD1 reduces nonheme iron transport into the enterocytes, which was mediated by the divalent metal transporter 1 protein. Daily consumption of fru...

Background: Asian pigeonwings flower (Clitoria Ternatea L.) is a plant that contains high antioxidants. Numerous research studies have shown that CT flowers can reduce the blood glucose levels of diabetic rats. Lower blood glucose levels can reduce MDA in DM patients. Objectives: This study aimed to analyze the effect of CT on serum malondialdehyde...

Introduction : Sepsis causes significant damage to the endothelial glycocalyx layer, which albumin can protect against. Unfortunately, human albumin is quite expensive, so cheaper substitutes must be found without sacrificing effectiveness. Albumin extract Channa striata is one that is commonly used in Indonesia. Objectives : Conducted a non-inferi...

Background: Sepsis causes significant damage to the endothelial glycocalyx layer, which albumin can protect against. Unfortunately, human albumin is quite expensive, so cheaper substitutes must be found without sacrificing effectiveness. Albumin extract called snakehead fish extract (SHFE) is one that is commonly used in Indonesia It is also called...

Protein-energy deficiency (PED) is a major infant health problem in developing countries with high mortality rate. Inadequate food intake and infectious diseases are the main factors for PED. In clinical setting, branded foods are commonly used to treat the PED but these foods are expensive. Canna (Canna edulis Ker.), catfish (Pangasius sp.), and r...

Patients with obesity often fail to control their body weight through diet and physical activity, while the existing anti-obesity drugs have some side effects for long-term use. Banana is Indonesia's tropical fruits with the highest production, and the banana peel has become soil waste. This study aimed to analyze nutrients and active compounds of...

Obesity is a complex chronic disease that becomes the main factor of cardiometabolic diseases such as heart diseases, stroke and diabetes mellitus. Oxidative stress is involved in the obesity pathogenesis. Diet and physical exercise are recommended for obesity treatment but many obese people have low compliance to these treatments. Sweet orange (Ci...

Anemia remains a global nutritional problem, which prevalence continues to increase every year. Children, female adolescents, and pregnant women are more susceptible to anemia than other age groups. Prolonged anemia has negative impacts on their life such as premature birth, low birth weight (LBW) babies and intrauterine fetal death. Snake fruit Po...

p> Pendahuluan: Jumlah kasus penyakit kardiovaskular (PKV) di dunia menunjukkan kecenderungan meningkat termasuk di Indonesia. Kabupaten Sukoharjo di Jawa Tengah melaporkan adanya peningkatan penyakit hipertensi dan diabetes mellitus (DM) yang menjadi faktor risiko penting PKV. Penyakit hipertensi dan DM merupakan penyakit penyerta utama pada kasus...

Background and objectives: Intestinal leakage commonly occurs in severe dengue infection with zonulin as a biomarker. The aim of this study was to determine the effects of NS1 on liver weight, zonulin expression and serum zonulin levels. Materials and methods: This laboratory experiment used 18 ddY mice, which were randomly divided into control...

Background: Toxoplasmosis is a protozoan disease caused by Toxoplasma gondii that can cause fetal abortion, stillbirth, death, or fetal and neonatal abnormalities. Spiramycin prophylactic purpose is to prevent fetal infection in toxoplasmosis. While Moringa oleifera contains bioactive compounds that benefit from treating oxidative stress and infect...

Visceral adipose tissue-derived serine protease inhibitor (vaspin) is an adipokine associated with insulin resistance (IR), obesity, and inflammation. Serum vaspin role and significant alteration in its levels in type 2 diabetes mellitus (T2DM) and atherosclerotic disease have been severally reported. Nevertheless, these immense changes and this ro...

BACKGROUND: The prevalence of obesity in adolescents has increased worldwide for recent years. Body mass index (BMI)/age, waist circumference (WC), and waist-to-height Ratio (WHtR) are commonly used for assessment of nutritional status. Daily intake of fruits and vegetables and eating time of fruits inversely associated with obesity. AIM: This stud...

The incidence of diabetes increased significantly around the world in accordance with lifestyle and change in eating behaviour. Streptozotocin-Nicotinamide (STZ-NA) is capable of inducing Diabetes Mellitus type 2 in experimental animals for insulin resistance. In this research, we inspect the therapeutic potential of Etlingera elatior ethanol extra...

Objectives: A high prevalence of tobacco smoking contributes to a high incidence of acute ischemic stroke (AIS) in Indonesia. Large-artery atherosclerosis is known to be a significant cause of AIS. The present study was aimed at evaluating the association between AIS and atherosclerosis on the basis of carotid intima-media thickness (CIMT) measure...

Abstrak Diabetes mellitus (DM) merupakan penyakit tidak menular (PTM) dengan prevalensi yang cukup tinggi di Kabupaten Karanganyar, Jawa Tengah. Pengendalian gula darah merupakan faktor utama untuk mencegah berbagai komplikasi penyakit DM. Tujuan dari pengabdian masyarakat ini adalah mengaktifkan gerakan masyarakat hidup sehat (Germas) dalam upaya...

Background As serious fracture complications, delayed union and non-union are parts of complications from fracture healing. Growth factors such as BMP-2, VEGF, proinflammatory cytokines including IL-6 and bone formation BALP, N-Mid-OC are important regulators of the fracture healing process. Red yeast rice (RYR), produced by fermenting Monascus pur...

Background: Type 2 Diabetes Mellitus (T2DM) is the prominent public health issue. Pharmacotherapy and diet modification should be integrated into T2DM management. Aims: To investigate the effects of vegetables consumption before carbohydrates on blood glucose and GLP-1 levels in T2DM patients. Methods: A non-randomized quasi experimental study...

BACKGROUND: There is a strong negative relationship between high-density lipoprotein cholesterol (HDL-C) and the risk of cardiovascular disease (CVD). Cholesterol ester transfer protein (CETP) is a glycoprotein transporter that transfers cholesterol esters to very low-density lipoprotein and low-density lipoprotein cholesterol (LDL-C). The CETP inh...

BACKGROUND:HDL has been identified as a potential new treatment for atherosclerosis. Targeting lipid metabolism via the Reverse Cholesterol Transport (RCT) pathway can improve HDL metabolism. Apolipoprotein A-I mimetic peptides (ApoA-I MPs) are able to increase HDL metabolism. Thus, this systematic review aimed to examine the potential effect of Ap...

BACKGROUND: Traumatic brain injury (TBI) can lead to cell death and neurologic dysfunction. Meanwhile, Remifentanyl is an opioid with potent analgesia, while magnesium sulfate (MgSO4) has antinociceptive properties that can prevent hemodynamic instability during laryngoscopy. AIM: This study aims to examine the effect of remifentanil, MgSO4 and the...

In this study, we investigated the effects of an alkaloid fraction of Mirabilis jalapa L. flowers in terms of cytotoxicity, Erythropoietin (EPO), hepcidin, and Matriptase-2 (MT-2) expression levels in iron deficiency Hepatocarcinoma (HepG2) cell model. The HepG2 cells with iron deficiency were induced by Deferoxamine (DFO) and were treated with sta...

BACKGROUND: Recently, sepsis has become a serious problem worldwide. There are many studies trying to find the etiologies of morbidity and mortality of sepsis. One of them is the damage of endothelial glycocalyx layer, which can lead to an increase in plasminogen activator inhibitor-1 (PAI-1) level and a decrease in platelets. This damage can be pr...

Introduction: Anemia is a globally nutritional problem and taking iron tablets regularly is recommended. Meanwhile, bay leaves are widely used for food flavoring, but only limited for anemia treatment. This study aimed to investigate the subchronic effects of bay leaves extract on body weight (BW), physical changes, and mortality in female Wistar r...

Objectives: Type 2 diabetes mellitus (T2DM) is a major global public health issue. Diet and physical exercise are modifiable factors that influence the glycaemic status of patients with T2DM. We aimed to investigate the acute effects of breakfast fruits meal sequence and postprandial exercise on the blood glucose level and dipeptidyl peptidase 4 (...

Introduction: Exercise has been believed to be an important step in treating and preventing Type 2 Diabetes Mellitus complications. The circadian rhythm influences systems in the body, including antioxidants in the human body. By synchronizing exercise with exercise time, it will maximize the benefits of exercise for health. Aim: Examining the effe...

Ginkgo biloba (GB) has anxiolytic properties, which can help to prevent the spread of vitiligo lesions. The purpose of this study was to examine the efficacy of adding orally and placebo GB extract as adjuvant therapy in treating depigmented lesions in vitiligo patients. This research is an experimental study with samples of vitiligo patients at th...

Butterfly pea flower (Clitoria ternatea L.) and lemon (Citrus limon) have bioactive compounds such as flavonoids and anthocyanin that were known the potential to be processed as functional beverage for health. The combination of them was never been done before. This experiment aimed to analyze the levels of total flavonoids, anthocyanin, and antiox...

Mice used as experimental animals in the laboratory belong to the genus Mus, subfamily Murinae, family Muridae, superfamily Muroidea, order Rodentia, and class M. musculus , these mice are also referred to as home mice. Mice have been used as standard animals in toxicology, teratology, and carcinogenesis tests, even today, mice have also been used...

Introduction : Prevalence of anemia among young women in Indonesia has been seen to increase in the last five years. This situation indicates that this nutritional disorder looks like an iceberg phenomenon. Daily consumption of vegetables containing foods is recommended for prevention of non-communicable diseases including anemia. These foods conta...

Allergic asthma is a chronic inflammatory disorder characterized by immune system changes. Allergen inhalation, which causes hyperactivity, eosinophil recruitment, mast cells, and lymphocytes in the upper and lower respiratory tracts, triggers the inflammation cascade and results in local and systemic inflammation responses. However, the mechanism...

Introduction: Psoriasis vulgaris is a chronic skin disease which is characterized by recurrent scales on skin. The global prevalence of this disease has increased in ten years. Plumbagin is an active compound in the P. zeylanica Linn. Some recent studies revealed that P. zeylanica Linn extracts have the antiproliferative activity, which is used for...

BACKGROUND: Obesity is the main risk factor of diabetes by which induces insulin resistance. Epicatechin gallate can virtually interact with sodium-glucose co-transporter 2 as same as dapagliflozin and is found in green tea and star fruits. AIM: This study aimed to investigate the effects of methanol extract of star fruit (MES) on body weight (BW),...

Objective: Lung cancer is the leading cause of death among cancer patients. The majority of lung cancer is the Non-Small Lung Carcinoma (NSLC). This study evaluated the potency of brazilin isolated from Caesalpinia sappan wood to induce apoptosis on non-small lung carcinoma cell line, A549, by examining the expression of p53, caspase-9, and caspas...

ABSTRAK Penyakit Tidak Menular (PTM) merupakan penyebab kematian tertinggi di dunia, termasuk di Indonesia. Salah satu PTM yang cukup tinggi prevalensinya adalah hipertensi. Kabupaten Karanganyar merupakan salah satu kabupaten di provinsi Jawa Tengah dengan jumlah kasus hipertensi yang cukup tinggi dan prevalensinya terus meningkat dari tahun ke ta...

Papaya (Carica papaya L) is one of the fruits that is easily found in Asian countries. Papaya has been studied to have good benefits in hyperlipidemia. Papaya contains fiber, vitamins A and C, alkaloids, saponins, and flavonoids which are with antihyperlipidemic properties. The objective of this research was to systematically review studies tha...

Introduction: Plumbago zeylanica grows widely in many tropical countries. In Indonesia, this plant, known as Daun Encok, has some beneficial effects on human health. Aim: This exploration study aimed to identify the plumbagin compound in P. zeylanica roots from Indonesia. Materials and methods: Dried roots of P. zeylanica were manually ground and...

Iron Deficiency Anemia (IDA) in female adolescents is a health problem that occupies the 8th position as a cause of global disability and is the common cause of anemia in reproductive women. Iron tablet supplementation is the government program for the depletion of IDA prevalence in Indonesia but often results in gastrointestinal side effects. Jama...

Hemophilia A is an inherited bleeding disorder caused by factor VIII (FVIII) deficiency. In most minor-to-moderate bleeding events, FVIII concentrates therapy can result in significant lifesaving. However, chronic FVIII concentrates therapy can cause FVIII inhibitors synthesis and replacement therapy to be inefficacious. Immunosuppressive therapy c...

Background: Fruit-infused water (FIW) contains antioxidants and potassium as a antihyperglycemic and antihypertension via inhibition or break the chain of radical reaction but has not been proven in patients with MS. Purpose: To evaluate the effects of FIW on blood pressure and fasting blood glucose (FBG) in patients with MS. Method: This is a Ra...

Nutritional problem that occurs in adolescents due to higher food intake and less physical activity leads to over- nutrition. Other nutritional problems occur among them is micronutrient deficiency including anemia, mostly in female adolescents. Higher consumption of energy-dense snacks, low physical activity and more pocket money have contributed t...

The elderly usually accompanied by many impairments due to decline in organ functioning which have a great impact on their daily intake and leads to anemia. Many elderlies also have mobility impairment which make them become more dependent and need supports from their family. The aim of this study is to investigate the relationship of family suppor...

Anthrax is a common zoonotic infection caused by Bacillus anthracis. In Indonesia, the incidence of anthrax is increasing and made this disease is one of the priorities of the 14 zoonotic disease elimination programs in Indonesia. Antibiotic is widely used to prevent target organ dysfunction in individuals exposed to anthrax. However, antibiotic th...