Donato Romano

• PhD
• Professor (Assistant) at Sant'Anna School of Advanced Studies

This paper introduces Animal Recognition Through Enhanced Multimodal Integration System (ARTEMIS), a transformer-based framework designed for multilabel animal action recognition by fusing video, image, and textual modalities. ARTEMIS utilizes state-of-the-art captioning and language models, such as BLIP2 and Llama 3, to generate textual descriptio...

Social immunity involves collective defensive strategies against infectious diseases. Despite its prevalence in eusocial insects, little is known about social immunity in non‐eusocial organisms like gregarious locusts. To address this gap, an emergent biohybrid approach bridging robotics and ethology is employed to study the behavior of the gregari...

Animal-robot interaction (ARI) is an emerging field that uses biomimetic robots to replicate biological cues, enabling controlled studies of animal behavior. This study investigates the potential for ARI systems to induce local enhancement (e.g., where animals are attracted to areas based on the presence or actions of conspecifics) in the Mediterra...

Interactively teaming up animals and robots could facilitate basic scientific research and address environmental and ecological crises.

The maize crop is highly susceptible to damage caused by its primary pests, which poses considerable challenges in manually identifying and controlling them at various larval developmental stages. To mitigate this issue, we propose an automated classification system aimed at identifying the different larval developmental stages of 23 instars of 4 m...

BACKGROUND Acrylamide‐based bait has super water absorption making it highly attractive to subterranean termites that are lured by wood with high water content. This study investigated the control efficiency of these baits on subterranean termites. In particular, we evaluated the water‐absorption capacity, attractiveness to subterranean termites, a...

Entomopathogenic nematodes (EPNs) are effective biocontrol agents, reducing pesticide impact on health and the environment. Understanding their physiology and ethology is crucial for optimizing their application. This study offers innovative insights about Steinernema carpocapsae EPN behavior, contributing to the interdisciplinary field of engineer...

Understanding the biology and ecology of parasitoids can have direct implications for their evaluation as biological control agents, as well as for the development and implementation of mass-rearing techniques. Nonetheless, our current knowledge of the possible influence of lateralized displays (i.e., the asymmetric expression of cognitive function...

Ostracods are small, bivalved crustaceans living in all types of aquatic habitats. All non-marine species belong to the order Podocopida. They usually possess a simple optical system composed of three eyecups called naupliar eye. Phylogenetic data and morphological characteristics support the homology of naupliar eyes between ostracods and other cr...

Entomopathogenic nematodes (EPNs) are organisms that are often mass-produced as biological control agents (BCAs) to mitigate pesticide-related hazards and foster environmental sustainability. Enhancing our understanding of EPNs biology and their interactions with hosts is crucial for refining the use of EPNs in integrated pest management. This stud...

Animal behavior serves as a reliable indicator of the adaptation of organisms to their environment and their overall well-being. Through rigorous observation of animal actions and interactions, researchers and observers can glean valuable insights into diverse facets of their lives, encompassing health, social dynamics, ecological relationships, an...

Dengue fever is a viral disease caused by one of four dengue stereotypes (Flavivirus: Flaviviridae) that are primarily transmitted by Aedes albopictus (Skuse) and Aedes aegypti (L.). To safeguard public health, it is crucial to conduct surveys that examine the factors favouring the presence of these species. Our study surveyed 42 councils across fo...

Bioinspired underwater robots can move efficiently, with agility, even in complex aquatic areas, reducing marine ecosystem disturbance during exploration and inspection. These robots can improve animal farming conditions and preserve wildlife. This study proposes a muscle-like control for an underactuated robot in carangiform swimming mode. The art...

Lateralisation is a well-established phenomenon observed in an increasing number of insect species. This study aims to obtain basic details on lateralisation in courtship and mating behaviour in Ostrinia furnacalis, the Asian corn borer. We conducted laboratory investigations to observe lateralisation in courtship and mating behaviours in adult O....

The field of animal–robot and organism–robot interaction systems (ARIS, ORIS) is a currently rapidly emerging field in biorobotics. In this special issue we aim for providing a comprehensive overview of the cutting-edge advancements and pioneering breakthroughs within this scientific and engineering discipline. Therefore, we collected scientific ar...

Social learning is an evolutionarily important ability increasingly attributed also to invertebrate species. Interfacing robots with animals represents a promising strategy to investigate social learning. Herein, we studied if the gregarious form of Locusta migratoria, a particularly suited model to examine social learning, can use social informati...

Rapidly intensifying global warming and water pollution calls for more efficient and continuous environmental monitoring methods. Biohybrid systems connect mechatronic components to living organisms and this approach can be used to extract data from the organisms. Compared to conventional monitoring methods, they allow for a broader data collection...

Orthoptera are insects with excellent olfactory sense abilities due to their antennae richly equipped with receptors. This makes them interesting model organisms to be used as biosensors for environmental and agricultural monitoring. Herein, we investigated if the house cricket Acheta domesticus can be used to detect different chemical cues by exam...

Animal-based sensors have been increasingly applied to many water monitoring systems and ecological studies. One of the staple organisms used as living sensors for such systems is Daphnia. This organism has been extensively studied and, with time, used in many toxicological and pharmaceutical bioassays, often used for exploring the ecology of fresh...

Pesticide exposure, even at low doses, can have detrimental effects on ecosystems. This study aimed at validating the use of machine learning for recognizing motor anomalies, produced by minimal insecticide exposure on a model insect species. The Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae), was exposed to food contaminated wi...

Biomimetic robotics can help support underwater exploration and monitoring while minimizing ecosystem disturbance. It also has potential applications in sustainable aquafarming management, biodiversity preservation, and animalrobot interaction studies. This study proposes a bio-inspired control strategy for an underactuated robotic fish, which util...

The Asian corn borer (Ostrinia furnacalis, Lepidoptera, Crambidae) and Oriental armyworm (Mythimna separata, Lepidoptera, Noctuidae) are 2 major lepidopteran pests of the maize plant, especially the whorls and tassels. The aim of this study was to investigate competition between 2 lepidopteran pests of maize. Intraspecific and interspecific competi...

Locusts keep their bodies moving in a straight line during the takeoff and maintain the body stable during the whole jumping with small pitching motions, ensuring both kinematic and dynamic stability to reach their intended destinations. Inspired by locusts' jumping performance, the Stephenson II six-bar jumping mechanism is adopted to mimic the ki...

Bioinspired robotics is a promising technology for minimizing environmental disruption during underwater inspection, exploration, and monitoring. In this research, we propose a control strategy for an underactuated robotic fish that mimics the oscillatory movement of a real fish’s tail using only one DC motor. Our control strategy is bioinspired to...

Pesticides are still abused in modern agriculture. The effects of their exposure to even sub-lethal doses can be detrimental to ecosystem stability and human health. This work aims to validate the use of machine learning techniques for recognizing motor abnormalities and to assess any effect post-exposure to a minimal dosage of these substances on...

Mass-rearing procedures of insect species, often used in biological control and Sterile Insect Technique, can reduce the insects competitiveness in foraging, dispersal, and mating. The evocation of certain behaviours responsible to induce specific neuroendocrine products may restore or improve the competitiveness of mass-reared individuals. Herein,...

A robotic beehive may unveil insights into honeybee collective behavior and sustain the colony in harsh weather.

The earthworms beneficial effects on soils may be promising to improve lunar soil fertility, enabling the use of local substrates for space farming. Herein, we investigated the effects of the lunar regolith simulant (LHS-1) at different concentrations in cow manure mixtures on the survival and fitness of Eisenia fetida. During 14 and 60-day experim...

Artificial Intelligence (AI) and automation are fostering more sustainable and effective solutions for a wide spectrum of agricultural problems. Pest management is a major challenge for crop production that can benefit from machine learning techniques to detect and monitor specific pests and diseases. Traditional monitoring is labor intensive, time...

In this study, a robotic fish inspired by carangiform swimmers is developed. The artifact presents a new transmission system that employs the magnetic field interaction of permanent magnets to ensure waterproofness and prevention from any overload for the structure and the actuating motor. This mechanism converts the rotary motion of the motor into...

In so-called ethorobotics and robot-supported social cognitive neurosciences, robots are used as scientific tools to study animal behavior and cognition. Building on previous epistemological analyses of biorobotics, in this article it is argued that these two research fields, widely differing from one another in the kinds of robots involved and in...

Collective behaviours in homogeneous shoals provide several benefits to conspecifics, although mixed-species aggregations have been reported to often occur. Mixed aggregations may confer several beneficial effects such as antipredator and foraging advantages. However, the mechanisms promoting phenotypically heterogeneous fish aggregations have been...

Fishes have evolved different excellent swimming strategies. To study the influence of tail fin swing on the swimming performance of bionic robot fish, with one joint under the same tail swing frequency and amplitude, we designed a novel robot fish, driven by a double-cam mechanism. By designing the profile of the cam in the mechanism, the robot fi...

Fish can swim in a variety of states. For example, they look flexible and perform low-frequency undulatory locomotion when cruising, but they seem very powerful and stiff and perform high-frequency undulatory when hunting. In the process of changing the motion state, the stiffness of the fish body affects the swimming performance of the fish. In th...

Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae) is a widely distributed key stored-product insect pest. Recent research efforts have been conducted on the laterality (i.e., left-right asymmetries in brain and behavior) during courtship and mating of coleopteran species that are related to stored products. However, our knowledge on the poten...

Lateralization has been documented in many insect species, but limited information on courtship and mating lateralization in wild conditions is available. We conducted field investigation on the courtship and mating behavior of the neem bug, Halys dentatus, a polyphagous insect mainly infesting Azadirachta indica, with particular attention to later...

Water monitoring is crucial for environmental monitoring, transportation, energy and telecommunication. One of the main problems in aquatic environmental monitoring is biofouling. The simplest method among the current antifouling strategies is the use of wiper technologies like brushes and wipers which apply mechanical pressure. In designing built-...

Welding defect detection is crucial to determine welding quality and assure correct mechanical performance of the welded joint during operation. Most of common detection techniques are performed offline, are time consuming, and strongly dependent on the operator skill. In the present work, two novel automatic online welding imperfections detection...

The emerging field of social robotics comprises several multidisciplinary applications. Anxiety and stress therapies can greatly benefit by socio-emotional support provided by robots, although the intervention of social robots as effective treatment needs to be fully understood. Herein, Paracheirodon innesi , a social fish species, was used to inte...

Miniaturised analytical systems are showing growing interest in aquatic ecology, although this technology has not been exploited to study cognitive behaviours of organisms in aquatic micro-environments. Herein, a miniaturised testing arena was developed to investigate information processes and learning of Heterocypris incongruens, a freshwater ostr...

Facing the threat of rapidly worsening water quality, there is an urgent need to develop novel approaches of monitoring its global supplies and early detection of environmental fluctuations. Global warming, urban growth and other factors have threatened not only the freshwater supply but also the well-being of many species inhabiting it. Traditiona...

Biorobotics is increasingly attracting engineers worldwide, due to the high impact this field can have on the society. Biorobotics aims at imitating or taking inspiration from mechanisms and strategies evolved by animals, including their locomotion abilities in real scenarios, such as swimming, running, crawling, and flying. However, the developmen...

Insects are important agents in ecosystems. Their diverseness and developed coping mechanisms also make them interesting for direct application and as a source of inspiration in medical engineering. We summarized the main contribution of insects in biomedical applications. Medical centers in North America, and Europe use fly larvae for maggot thera...

Apiaceae is a family encompassing medicinal and aromatic plants. They produce essential oils (EOs) inside oil canals, known as ducts and vittae, which occur in their vegetative and reproductive organs. Given the high EO yields and the availability of raw material from cultivations widespread all over the world, Apiaceae are exploitable for differen...

Group behaviours in fish are widespread, and are increasingly used to develop new optimisation algorithms for solving complex tasks. However, how external factors regulate individual spatial location in a fish school represents an overlooked aspect that is fundamental to understand collective dynamics. Herein, the neon tetra Paracheirodon innesi, a...

Pathogen transmission is a major limit of social species. Social distancing, a behavioural-based response to diseases, has been regularly reported in nature. However, the identification of distinctive stimuli associated with an infectious disease represents a challenging task for host species, whose cognitive mechanisms are still poorly understood....

Social learning represents a high-level complex process to acquire information about the environment, which is increasingly reported in invertebrates. The animal–robot interaction paradigm turned out to be an encouraging strategy to unveil social learning in vertebrates, but it has not been fully exploited in invertebrates. In this study, Lucilia s...

Sustainable, green energy harvesting has gained a considerable amount of attention over the last few decades and within its vast field of resources, bio-energy harvesters have become promising. These bio-energy harvesters appear in a wide variety and function either by directly generating energy with mechanisms similar to living organisms or indire...

The yellow mealworm, Tenebrio molitor L., is one of the most significant insect species of economic importance for producing protein-rich food and feed. The larvae are a promising fishmeal substitute for fish feed, and a good alternative source for human nutrition. In this study, the effect of behavioral asymmetries on male mating success of T. mol...

In the wake of climate change and water quality crisis, it is crucial to find novel ways to extensively monitor the environment and to detect ecological changes early. Biomonitoring has been found to be an effective way of observing the aggregate effect of environmental fluctuations. In this paper, we outline the development of biohybrids which wil...

Jumping is a locomotion strategy widely evolved in both invertebrates and vertebrates. In addition to terrestrial animals, several aquatic animals are also able to jump in their specific environments. In this paper, the state of the art of jumping robots has been systematically analyzed, based on their biological model, including invertebrates (e.g...

Sensory fusion is used by the males of several animal species to discriminate the mating status of females by evaluating their phenotypic traits. The predominant trait used is olfactory cues, and the role of visual cues is not yet fully understood. The ability of Poecilia reticulata males to evaluate females’ receptivity based on visual cues was in...

Ethorobotics, a new fascinating field of biorobotics, proposes the use of robotic replicas as an advanced method for investigating animal behaviour. This novel research approach can also encourage the development of advanced bioinspired robots. In the present study, we investigated the pushing behaviour, a particular display occurring in several be...

The lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae), is an important pest of stored grains globally. In this study, the impact of geographical origin on male mating success and lateralization in three strains of R. dominica (i.e., a Greek strain, a Romanian strain and a Turkish strain) was investigated. Concerning the Greek...

Medicinal and aromatic plants represent an outstanding source of green active ingredients for a broad range of real-world applications. In the present study, we investigated the insecticidal potential of the essential oils obtained from three medicinal and aromatic plants of economic importance in Algeria, Artemisia campestris, Pulicaria arabica, a...

Locusts generally live and move in complex environments including different kind of substrates, ranging from compliant leaves to stiff branches. Since the contact force generates deformation of the substrate, a certain amount of energy is dissipated each time when locust jumps from a compliant substrate. In published researches, it is proven that o...

Bacteria colonization of substrates is a key issue threatening the health of humans worldwide. This review summarized recent bactericidal activity evidences of different structures developed by insects. We evaluated different mechanisms in bactericidal activity among several insect species and we also provided a glimpse at attempts at fabricating b...

Phosphine (PH3) is the most commonly used fumigant to protect stored products from arthropod infestations worldwide. Our knowledge about the behavioral differences between phosphine-resistant and -susceptible stored product pest populations is limited. This study evaluated differences in mobility and behavior of populations of two major stored prod...

Ontogenetic locomotion research focuses on the evolution of locomotion behavior in different developmental stages of a species. Unlike vertebrates, ontogenetic locomotion in invertebrates is poorly investigated. Locusts represent an outstanding biological model to study this issue. They are hemimetabolous insects and have similar aspects and behavi...

Motor performance deficits are major issues impacting functional activities in older adults. Herein, we used locusts, emergent model organisms for neurodegeneration and motor deficit studies, to investigate the role of cognition as compensation strategy to age-related motor activation deficiency. An animal-robot interaction has been established to...

A major advantage of animal aggregations concerns cooperative antipredator strategies. Schooling behavior emerges earlier in many fish species, especially in those cannibalizing their offspring. Experience is fundamental for developing schooling behavior. However, the cognitive ability of naive newborn fish to aggregate remains unclear. Herein, Poe...

Campoplex capitator is an ichneumonid parasitoid with a narrow host range, comprising grapevine moth pests. Despite being considered one of the possible candidates for biocontrol of Lobesia botrana, knowledge about its biology is limited and mass‐rearing for commercial purposes is still lacking. This research provides a quantitative analysis of the...

Living organisms are far superior to state-of-the-art robots as they have evolved a wide number of capabilities that far encompass our most advanced technologies. The merging of biological and artificial world, both physically and cognitively, represents a new trend in robotics that provides promising prospects to revolutionize the paradigms of con...

Among territorial animals, several species are characterized by males showing the same initial behaviours towards both sexes, leading to significant chances of injuries against conspecifics. In this study, we investigated how visual stimuli exhibited by a female-mimicking robotic replica can be exploited by highly territorial Betta splendens males...

The development of effective and eco-friendly pesticides to manage mosquito vector larvae is a timely and crucial challenge nowadays. Insecticide resistance is on the rise, therefore plant-borne insecticides with multiple mode of action may represent promising candidates to control insect vectors. In the present work, we encapsulated selected essen...

Recently, the functional asymmetries of courtship and mating behavior in several major pests of economic importance at the post-harvest stages of agricultural commodities have been reported. In this study, we investigated the presence of population-level behavioral asymmetries in the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Teneb...

Locusts are widely recognized for their jumping performances. Jumping allows locusts to avoid predators and initiate flight. In this study, jumping performances of Locusta migratoria were modelled with a lumped-parameter theoretical model. Results showed that L. migratoria can reach a maximum take-off velocity of 1.83 m/s and maximum acceleration o...

The effect of previous exposure to lateral sensory stimuli in shaping the response to subsequent symmetric stimuli represents an important overlooked issue in neuroethology, with special reference to arthropods. In this research, we investigated the hypothesis to ‘programme’ jumping escape direction as well as surveillance orientation in young and...

Among insects, locusts are widely recognized for their excellent jumping performances. Indeed, jumping helps locusts to avoid predators as well as to initiate flight. In the present contribution, we employed high-speed videos to analyze the jumping performance of Locusta migratoria L. from ground with different toughness. Results outlined that L. m...

Among insects, locusts are widely recognized for their excellent jumping performances. Indeed, jumping helps locusts to avoid predators as well as to initiate flight. In the present contribution, we employed high-speed videos to analyze the jumping performance of Locusta migratoria L. from ground with different toughness. Results outlined that L. m...

Lucilia sericata is well known for causing myiasis in humans, livestock, pets and wildlife. It also vectors various pathogens, including paratuberculosis agents. This species can be exploited in maggot therapy to treat necrotic wounds, particularly those infected by multidrug-resistant pathogens. Despite the high medical and veterinary importance o...

This paper presents the research done in the field of robotic cultural evolution in challenging real world environments. We hereby present these efforts, as part of project subCULTron, where we will create an artificial society of three cooperating sub-cultures of robotic agents operating in a challenging real-world habitat. We introduce the novel...

Ticks are considered among the most dangerous arthropod vectors of disease agents to both humans and animals worldwide. Lateralization contributes to biological fitness in many animals, conferring important functional advantages, therefore studying its role in tick perception would critically improve our knowledge about their host-seeking behavior....

Calliphora vomitoria is a myiasis-causing fly in many animal species including humans. The control of blowflies is still anchored on the use of chemicals. However, mass trapping and lure-and-kill techniques represent a promising alternative to pesticides. Visual and olfactory cues are the main stimuli routing the fly's landing behavior. Notably, co...

Mating trophallaxis represents a fascinating strategy adopted by males of several animal species to affect the decision-making of females during mating. Among tephritids, few species perform mating trophallaxis. However, this phenomenon has been little studied in major tephritid pests. We reported the presence of indirect mating trophallaxis in Med...

Lateralization is a fundamental principle of the brain organization widespread among vertebrates but rather unknown in invertebrates. Evidences of lateralized courtship and mating behavioral traits in parasitic wasps are extremely rare. Here, courtship and mating sequences and the presence of mating lateralization in Anagyrus sp. near pseudococci,...

The use of robotics to establish social interactions between animals and robots, represents an elegant and innovative method to investigate animal behaviour. However, robots are still underused to investigate high complex and flexible behaviours, such as aggression. Here, Betta splendens was tested as model system to shed light on the effect of a r...

The recent Zika virus outbreaks have been recognized as “Public Health Emergency of International Concern” by WHO. In this review, we summarized current knowledge about Zika virus vector competence in mosquitoes. Aedes africanus was the first species where Zika virus was isolated. Currently, Aedes aegypti and Aedes albopictus are reported as the ma...

Escape and surveillance responses to predators are lateralized in several vertebrate species. However, little is known on the laterality of escapes and predator surveillance in arthropods. In this study, we investigated the lateralization of escape and surveillance responses in young instars and adults of Locusta migratoria during biomimetic intera...

Ticks serve as vectors of a wide range of infectious agents deleterious to humans and animals. Tick bite prevention is based to a large extent on the use of chemical repellents and acaricides. However, development of resistance in targeted ticks, environmental pollution, and contamination of livestock meat and milk are major concerns. Recently, met...

In the last decade, the growing interest in underwater vehicles allowed significant progress in underwater robotic missions. Despite of this, underwater habitats remain one of the most challenging environments on earth due to their extreme and unpredictable conditions. The development of underwater platforms for environmental monitoring raises seve...