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Dominique Tremblay

Dominique Tremblay
Agile MV · R&D

PhD -- www.domtremblay.net

About

32
Publications
4,357
Reads
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539
Citations
Additional affiliations
January 2010 - June 2014
University of Ottawa
Position
  • Research Associate
Description
  • Conceived, design and built unique laboratory instruments for applications in cell biology and tissue engineering.
September 2005 - December 2009
McGill University
Position
  • Research Assistant
Description
  • Conceived, planned and conducted a multi-disciplinary research project on the assessment of the mechanical properties and chemical composition of human and porcine ascending aortic tissues.
Education
September 2005 - November 2009
McGill University
Field of study
  • Chemical Engineering
September 2003 - September 2005
Polytechnique Montréal
Field of study
  • Mechanical Engineering
January 1999 - May 2003
Polytechnique Montréal
Field of study
  • Mechanical/Aeronautical Engineering

Publications

Publications (32)
Conference Paper
This research investigates the soil cutting problem with a flat blade excavating soil. Experiments identify and characterize oscillatory soil failure patterns, based on data including forces on the robotically-controlled excavation tool, as well as granular soil flows tracked via a high-speed camera and a novel computer vision-based technique. In t...
Conference Paper
Full-text available
This research investigates the development and validation of state-of-the-art high-fidelity models of soil cutting operations. The accurate and efficient modeling of complex tool-soil interactions is an open problem in the literature. Modeling options that provide more flexibility in trading off accuracy and computational efficiency than current st...
Article
Living cells possess an exquisite ability to sense and respond to physical information in their microenvironment. This ability plays a key role in many fundamentally important physiological and pathological processes. We will describe our work utilizing a variety of biophysical tools to investigate the dynamic responses of cells to mechanical stimu...
Article
Full-text available
Tools that allow the application of mechanical forces to cells and tissues or that can quantify the mechanical properties of biological tissues have contributed dramatically to the understanding of basic mechanobiology. These techniques have been extensively used to demonstrate how the onset and progression of various diseases are heavily influence...
Article
Physical forces arising in the cellular microenvironment have been hypothesized to play a major role in governing cell function. Moreover, it is thought that gene regulation may be sensitive to nuclear deformations taking place in response to extracellular forces over short and long timescales. Although nuclear responses to mechanical stimuli over...
Article
Full-text available
A multi-layered polydimethylsiloxane microfluidic device with an integrated suspended membrane has been fabricated that allows dynamic and multi-axial mechanical deformation and simultaneous live-cell microscopy imaging. The transparent membrane’s strain field can be controlled independently along two orthogonal directions. Human foreskin fibroblas...
Article
INTRODUCTION: Tissue strains can result in significant nuclear deformations and may regulate gene expression. However, the precise role of the cytoskeleton in regulating nuclear mechanics remains poorly understood. Here, we investigate the nuclear deformability of Madin-Darky canine kidney cells (MDCK) under various stretching conditions to clarify...
Article
Full-text available
Expression of Heat Shock Protein-27 (HSP27) is reduced in human coronary atherosclerosis. Over-expression of HSP27 is protective against the early formation of lesions in atherosclerosis-prone apoE(-/-) mice (apoE(-/-)HSP27(o/e)) - however, only in females. We now seek to determine if chronic HSP27 over-expression is protective in a model of advanc...
Article
Full-text available
Integrins, focal adhesions, the cytoskeleton and the extracellular matrix, form a structural continuum between the external and internal environment of the cell and mediate the pathways associated with cellular mechanosensitivity and mechanotransduction. This continuum is important for the onset of muscle tissue generation, as muscle precursor cell...
Article
BACKGROUND/INTRODUCTION: Diseases of the aorta can alter the local mechanical properties of the tissue, leading to aneurysms and plaque instability. Local tissue properties are best evaluated from surgical samples or autopsy tissue using mechanical testing ex vivo. We examined whether formalin-fixed tissues preserve regional and local variations in...
Article
INTROExtracellular forces can result in significant nuclear deformations and may modulate transcription activity. However, the precise role of the cytoskeleton network in regulating nuclear mechanics remains poorly understood. The goal of the present study is to investigate the nuclear deformability under complex substrate strain fields to clarify...
Article
Full-text available
Background: Previously, we discovered that over-expression of Heat Shock Protein 27 (HSP27) in atherosclerosis-prone apoE -/- mice (apoE -/- HSP27 o/e ) maintained on a high fat diet (HFD) for 4 weeks reduces aortic lesion area - however, only in females. Purpose/Objective: To determine if chronic HSP27 over-expression modulates plaque lesion chara...
Article
The human ascending aorta (AA) is exposed to very high shear and pressure stresses exerted by the blood flow ejected from the left ventricle outflow tract. This vessel has a unique structural behaviour which adequately redistributes the energy captured from the blood flow ejection to sustain a more continuous blood flow through the entire vascular...
Article
Dilation and dissection of aneurysmal ascending aortic tissues occur preferentially at the outer curvature of the vessel. In this study we hypothesize that the density and contractile properties of the vascular smooth muscle cells (VSMCs) of the pig ascending aorta (AA) are heterogeneous and could explain the non-uniform remodeling and weakening of...
Thesis
The exact mechanisms involved in the pathogenesis of aortic aneurysms of the human ascending aorta (AA) are unknown. It is believed that the remodeling capabilities of the endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) combined with abnormal and non-uniform shear and tissue stresses, generated by a perturbed hemodynamics produced...
Article
Differences in the mechanical properties of aortic tissues and replacement materials can have unwanted hemodynamic effects leading to graft failure. The aim of this experimental study was to compare the mechanical properties of different graft-patch materials used in aortic arch reconstruction with those of healthy and dilated human ascending aorta...
Article
This study investigates the mechanics and histology of healthy and dilated human ascending aortas (AA). The regional variation in mechanical response and tissue structure were compared. Rings of human AA from healthy (n=5), dilated tricuspid aortic valve (TAV, n=5), and dilated bicuspid aortic valve (BAV, n=6) patients were mechanically tested. Eac...
Conference Paper
Healthy and pathologic tissues of human ascending aorta (AA) were obtained from autopsy and surgical pathology. Each aortic ring was classified as a healthy (nondilated AA) or diseased (dilated AA) group. Within the diseased group, the samples were further sub-classified as tricuspid aortic valve (TAV), fused tricuspid aortic valve (fTAV) and conge...
Conference Paper
Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal
Conference Paper
Little information is available on the structure or mechanical properties of the human ascending aorta (AA). Most studies to date have assumed homogeneous tissue mechanical properties. The objective of this study was to investigate and correlate the local variation in AA tissue structure, and mechanical properties to better understand the developme...
Conference Paper
Full-text available
This paper presents the Method of Manufactured Solutions (MMS) for fuid-structure interactions code veri�cation. The MMS provides benchmark solutions for direct evaluation of the solution error. The best benchmarks are exact analytical solutions with su�ciently complex solution structure to ensure that all terms of the di�erential equations are exe...
Thesis
"Mémoire présenté en vue de l'obtention du diplôme de Maîtrise ès sciences appliquées (génie mécanique)" Thèse (M.Sc.A.) -- École polytechnique, 2006.
Article
This paper illustrates the use of an adaptive finite element method as a means of achieving verification of codes and simulations of impinging round jets, that is obtaining numerical predictions with controlled accuracy. Validation of these grid-independent solution is then performed by comparing predictions to measurements. We adopt the standard a...
Conference Paper
This paper presents a general formulation of the continuous sensitivity equation method for computing first order sensitivities for transient non-linear heat conduction problems. The development of general differential equations for the sensitivities is presented for the fully non-linear heat equation. Boundary conditions are developed for both sha...

Questions

Question (1)
Question
I'm doing time-lapse live-cell imaging of a monolayer of MDCKs for 24 hrs and 48 hrs. I'm monitoring cell migration over that period of time but, ideally, I would need to stop cell division in order to have the same number of cells in the microscope's field of view. I'm using DMEM supplemented with 1% FBS, which synchronize the cells by stopping division. However I still get division to some extent. Are there any alternative ways to stop division?

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