Dominique Melck

Dominique Melck
Italian National Research Council | CNR

About

110
Publications
8,786
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
6,744
Citations

Publications

Publications (110)
Article
Full-text available
It is still debated whether non-specific preclinical symptoms of Alzheimer's disease (AD) can have diagnostic relevance. We followed the evolution from cognitively normal to AD by NMR-based metabolomics of blood sera. Multivariate statistical analysis of the NMR profiles yielded models that discriminated subjective memory decline (SMD), mild cognit...
Article
Full-text available
Enzalutamide (MDV3100) is a potent second-generation androgen receptor antagonist approved for the treatment of castration-resistant prostate cancer (CRPC) in chemotherapy-naïve as well as in patients previously exposed to chemotherapy. However, resistance to enzalutamide and enzalutamide withdrawal syndrome have been reported. Thus, reliable and i...
Article
Full-text available
Introduction In fruits and vegetables, comparative analysis of metabolic plant profiles has a high potential for quality control of active components. Onion (Allium cepa L.) is used fresh or stored as food, spice, and in traditional medicine. Its metabolic content, often with nutraceutical value, makes its level an important factor in agronomic pro...
Article
In this study, we utilized a model of trout intestinal explants to investigate the uptake and the metabolic effects of Hydroxytyrosol (HT), a bioactive molecule belonging to polyphenols, present in the fruit and leaf of the olive (Olea europaea L.). Fragments of the pyloric caeca (PC), mid intestine (MI) and distal intestine (DI) of the rainbow tro...
Article
Exhaled Breath Condensate (EBC) is a non-invasive biological matrix that allows easy access to the lung epithelial lining fluid. It can provide, for instance, useful biochemical information in workers following inhalatory exposure. Recently, nuclear magnetic resonance (NMR)-based metabolomics has been applied to EBC profiling to identify metabolic...
Article
Full-text available
15-F2t-Isoprostane, a reliable biomarker of oxidative stress, has been found elevated in exhaled breath condensate (EBC), a non-invasive technique for sampling of airway secretions, in patients with cystic fibrosis (CF). Azithromycin has antioxidant properties in experimental models of CF, but its effects on oxidative stress in CF patients are larg...
Article
Ammonia is a potent neurotoxin that is detoxified mainly by the urea cycle in the liver. Hyperammonemia is a common complication of a wide variety of both inherited and acquired liver diseases. If not treated early and thoroughly, it results in encephalopathy and death. Here, we found that hepatic autophagy is critically involved in systemic ammoni...
Data
Document S1. Supplemental Experimental Procedures, Figures S1–S3, and Tables S4–S6
Data
Table S1. List of Differentially Methylated Regions; Genes Whose Promoters Are Differentially Methylated; DMR-Associated Genes in l-Pro- and VitC-Treated ESCs, Related to Figure 1
Data
Table S2. List of Differentially Expressed Genes and of Pluripotency-Associated and Priming Markers in 2i-, VitC-, l-Pro-, and F/A-Treated, and Untreated Cells, Related to Figure 2
Data
Table S3. List of Differentially Expressed Genes; Genes Related to Pluripotency, Mesendodermal Specification, and ERK Superpathway Differentially Expressed in F/A- and l-Pro-Treated Cells, Related to Figure 3
Article
Full-text available
Metabolites and cofactors are emerging as key regulators of cell plasticity and reprogramming, and their role in the control of pluripotency is just being discovered. Here we provide unprecedented evidence that embryonic stem cell (ESC) pluripotency relies on the relative levels of two physiological metabolites, namely ascorbic acid (vitamin C, Vit...
Article
Background: Epidemiological and clinical evidence supports the existence of an obesity-related asthma phenotype. No distinct pathophysiological elements or specific biomarkers have been identified so far but increased oxidative stress has been reported. Objective: We aimed at verifying whether metabolomics of exhaled breath condensate (EBC) from...
Article
Investigations of young shoots of Aruncus dioicus (Walter) Fernald var. vulgaris (Maxim.) H.Hara (Rosaceae), collected from the wild and used as vegetables in alpine provinces of Italy, yielded eight monoterpenoids. Besides known compounds, aruncin A, aruncide A, and cimicifugolide, five previously undescribed substances, aruncins C, D, and E, and...
Article
Among various nanoparticles the gold derivatives (AuNPs) possess many features such as ease of synthesis at a tunable size, chemical stability and flexibility to undergo surface modifications, thus making them good candidates as bio-carrier across central nervous system and target diseased cells. However, a significant gap remains in data regarding...
Article
Among various nanoparticles the gold derivatives (AuNPs) possess many features such as ease of synthesis at a tunable size, chemical stability and flexibility to undergo surface modifications, thus making them good candidates as bio-carrier across central nervous system and target diseased cells. However, a significant gap remains in data regarding...
Article
Full-text available
Introduction Molecular factors are differentially observed in various bent sectors of poplar (Populus nigra) woody taproots. Responses to stress are modulated by a complex interplay among different hormones and signal transduction pathways. In recent years, metabolomics has been recognized as a powerful tool to characterize metabolic network regula...
Article
Prostate cancer is highly sensitive to hormone therapy because androgens are essential for prostate cancer cell growth. However, with the nearly invariable progression of this disease to androgen independence, endocrine therapy ultimately fails to control pros-tate cancer in most patients. Androgen-independent acquisition may involve neuroendocrine...
Article
Prostate cancer is highly sensitive to hormone therapy because androgens are essential for prostate cancer cell growth. However, with the nearly invariable progression of this disease to androgen independence, endocrine therapy ultimately fails to control prostate cancer in most patients. Androgen-independent acquisition may involve neuroendocrine...
Article
Prostate cancer is highly sensitive to hormone therapy because androgens are essential for prostate cancer cell growth. However, with the nearly invariable progression of this disease to androgen independence, endocrine therapy ultimately fails to control prostate cancer in most patients. Androgen-independent acquisition may involve neuroendocrine...
Article
Full-text available
In humans, obesity plays a critical role in pulmonary inflammation, and is an important risk factor for the development of several inflammatory respiratory diseases. Therefore, understanding the mechanisms that generate mediators in the setting of obesity may help to recognize the link between obesity and chronic respiratory diseases, like the incr...
Article
Prostate cancer (PCa) is highly sensitive to hormone therapy because androgens are essential for prostate cancer cell growth. However, with the nearly invariable progression of this disease to androgen independence (AI), endocrine therapy ultimately fails to control PCa in most patients. AI acquisition may involve neuroendocrine (NE) transdifferent...
Article
A NADP(+) dependent dehydrogenase activity on 3-glutathionyl-4-hydroxynonanal (GSHNE) was purified to electrophoretic homogeneity from a line of human astrocytoma cells (ADF). Proteomic analysis identified this enzymatic activity as associated with carbonyl reductase 1 (E.C. 1.1.1.184). The enzyme is highly efficient at catalyzing the oxidation of...
Article
Obesity and associated metabolic syndrome have quickly become a pandemic and a major detriment to human health globally. The presence of non-alcoholic fatty liver disease (NAFLD; hepatosteatosis) in obesity has been linked to the worsening of the metabolic syndrome, including the development of insulin resistance and cardiovascular disease. Current...
Article
Exhaled Breath Condensate (EBC) collection is a non-invasive method to investigate lung diseases. EBC is usually collected with commercial/custom-made condensers, but the optimal condensing temperature is often unknown. As such, the physical and chemical properties of exhaled metabolites should be considered when setting the temperature, therefore...
Article
Synaptosomal protein synthesis from rat brain is selectively increased by learning and is massively enhanced during the recovery period from brain ischemia. To lay the groundwork for identification of the involved synaptic elements, we examined the effects induced by varying the concentrations of extracellular cations and endogenous calcium. Most o...
Article
Tyr) (maculosin-1, (3S,8aS)-3-(4-hydroxybenzyl)hexahydropyrrolo[1,2-a]pyrazine-1,4-dione (1)), a host-specific phytotoxin, was isolated, together with some analogous cyclic dipeptides, from Alternaria alternata, the causal agent of black blight of spotted knapweed [1]. Compound 1 was tested against 19 plant species, and it showed phytotoxic activit...
Article
Full-text available
Hepatocellular carcinoma (HCC) is a very aggressive neoplasia requiring early and accurate diagnosis to improve patient outcomes with timely treatment. The liver is also very frequently colonized by metastases, and the most frequent differential diagnosis is HCC against intrahepatic cholangiocarcinoma or metastatic adenocarcinoma. Metabolomics is a...
Article
Full-text available
The economically important crop Juniperus communis (Cupressaceae) is a coniferous evergreen shrub or tree distributed throughout the temperate zone of the Northern hemisphere. Its dried bluish-black cones, known as “juniper berries”, are used as flavoring agent in foodstuffs and spirits, while their extracts are widely employed in medicine for thei...
Article
Recently in European countries, control laboratories have revealed real cases of deceptive practices in which synthetic triacylglycerols (TAGs) were used for adulterating butterfat. The complex fat blends are difficult to detect with the classical methods of analysis, especially when the adulterating TAGs contain fatty acids (FAs) in the proportion...
Article
An aggressive isolate of Ascochyta lentis obtained from lentils (Lens culinaris L.) produced various metabolites in vitro. The metabolites were isolated from the culture filtrates and characterized by spectroscopic, chemical and optical methods. A new phytotoxic anthraquinone, named lentisone, was isolated and characterized as (1S*,2S*,3S*)-1,2,3,8...
Article
Nuclear Magnetic Resonance (NMR)-based metabolomics separates Exhaled Breath Condensate (EBC) profiles of patients affected by pulmonary disease from healthy subjects. Here we show the discriminatory ability of NMR-based metabolomics in separating patients exposed to the same risk factor, namely, smoking habit in smoking-related diseases. Fifty dup...
Article
Full-text available
Hexactinellids (glass sponges) are an understudied class with syncytial organization and poor procariotic associations, thought to lack defensive secondary metabolites. Poriferans, though, are outstanding sources of bioactive compounds; nonetheless, a growing suspicion suggests that many of these chemicals could be symbiont-derived. In Polar latitu...
Article
Full-text available
To the Editors: Due to the lack of standardised procedures for exhaled breath condensate (EBC), a noninvasive technique for investigating lung inflammatory mediators [1], the between-laboratory comparison of results is difficult. Moreover, different collecting devices have been reported to influence the EBC content [2, 3]. The analysis of metabol...
Article
Full-text available
Metabolomics could provide new insights into the pathophysiology of cystic fibrosis (CF) by identifying profiles of endogenous metabolites. To investigate whether metabolomics of exhaled breath condensate could discriminate between patients with unstable CF, stable CF and healthy subjects, and whether selected metabolites were responsible for betwe...
Article
By using NMR spectroscopy, a non-invasive investigation technique, we performed in vivo experiments aimed at uncovering the metabolic pathways involved in the early response of Fabrea salina cells to ultraviolet (UV) radiation. This hypersaline ciliate was chosen as a model organism because of its well-known high resistance to UV radiation. Identic...
Article
A strain of Botrytis fabae isolated from faba bean (Vicia faba L.) plants displaying clear chocolate spot disease symptoms produced phytotoxic metabolites in vitro. The phytotoxins isolated from the culture filtrate organic extract were characterized by spectroscopic and optical methods. A new naphthalenone pentaketide, named botrytone, was isolate...
Article
Orobanche and Phelipanche species (the broomrapes) are root parasitic plants, some of which represent serious weed problems causing severe yield losses on important crops. Control strategies have largely focused on agronomic practices, resistant crop varieties and herbicides, albeit with marginal success. An alternative control method is the induct...
Article
Full-text available
There is increasing evidence that biomarkers of exhaled gases or exhaled breath condensate (EBC) may help in detecting abnormalities in respiratory diseases mirroring increased, oxidative stress, airways inflammation and endothelial dysfunction. Beside the traditional techniques to investigate biomarker profiles, “omics” sciences have raised intere...
Article
Fabrea salina is a hypersaline ciliate that is known to be among the strongest ultraviolet (UV)-resistant microorganisms; however, the molecular mechanisms of this resistance are almost unknown. By means of in vivo NMR spectroscopy, we determined the metabolic profile of living F. salina cells exposed to visible light and to polychromatic UV-B + UV...
Article
In agreement with the highly lipophilic nature of (-)-delta9-tetrahydrocannabinol, all the endogenous ligands of cannabinoid receptors identified so far are derivatives of long chain fatty acids. N- Arachidonoylethanolamine (anandamide) and some of its polyunsaturated congeners have been found in mammalian brain and shown to activate the CB1 and, w...
Article
Full-text available
Human hepatocellular carcinoma (HCC) is the most recurrent malignancy of the liver and represents one of the main causes of cancer death worldwide. Furthermore, the liver is the most frequent site of metastatic colonization, and hepatic metastases are far more common than primary cancers in Western countries. A possible way of investigating liver d...
Article
Lipophilic phytotoxins produced by Neofusicoccum parvum, a Botryosphaeriaceae species pathogenic to grapevine, were isolated and identified by spectroscopic methods as (3R,4R)-(-)-4-hydroxymellein, (3R,4S)-(-)-4-hydroxymellein, isosclerone and tyrosol. When assayed for phytotoxicity on tomato plants, all four metabolites showed phytotoxic activity,...
Article
Living cell metabolism is often monitored by 1D NMR spectroscopy, but the spectral resolution and the short cell lifetime are certainly limiting aspects. 2D spectroscopy does yield higher resolution but is time-consuming since acquisition of the second dimension requires several minutes. However, after only few minutes, oxygen starvation changes ce...
Article
Anomerically free acidic xylo-oligosaccharides have shown interesting biological properties when tested against Gram-positive and Gram-negative aerobically grown bacteria, as well as against Helicobacter pylori, sarcoma-180 and other tumors. We report here a structure–activity relationship study on the role of 4-O-methyl glucuronic acid (MeGlcUA) i...
Article
A strain of Ascochyta agropyrina var. nana was isolated from Elytrigia repens (quack grass), a noxious perennial weed widespread through the cold regions of the northen and southern hemispheres. Papyracillic acid was isolated for the first time from the fungal solid culture and identified using spectroscopic methods, including X-ray diffractometric...
Article
Cafestol and kahweol, coffee-specific furan diterpenes, are believed to cause various physiological effects in human subjects, including an increase in cholesterol and plasma triacylglycerol levels as well as cancer chemopreventive effects. Despite the increasing interest in these compounds raised by the diverse range of biological activities, thei...
Article
Six unreported aromatic compounds, 1-6, were isolated, along with the known compounds dehydrocurcuphenol and manoalide, from a sample of Plakortis sp., which was the main component of a Pacific sponge consortium. The new molecules were chemically characterized by spectroscopic methods. Compounds 1-4 contain a six-membered cyclic peroxide, whereas 5...
Article
A 3,5-disubstituted furan, named flufuran, was isolated from a culture filtrate of a strain of Aspergillus flavus obtained from a chestnut compost created in the same orchard. Flufuran was identified by spectroscopic methods, and its structure was confirmed through the preparation of some key derivatives, also used to test the antifungal activity....
Article
This paper reports the chemical study of a sample of Fasciospongia sp. collected along the Atlantic Portuguese coast. Three new isomeric furanosesquiterpenes, isomicrocionin-3 ( 1), (-)-microcionin-1 ( 2), and (-)-isomicrocionin-1 ( 3), were isolated along with the known (-)- ent-pallescensin A ( 4) and (-)-pallescensin-1 ( 5) from the ethyl acetat...
Article
Full-text available
Exhaled breath condensate (EBC) is a noninvasive method for the study of airway lining fluid. Nuclear magnetic resonance (NMR) spectroscopy can provide biochemical profiles of metabolites in biological samples. The aim of the present study was to validate the NMR metabonomic analysis of EBC in adults, assessing the role of pre-analytical variables...
Article
Two novel metabolites with potential antifeedant activity were isolated from cultures of the fungus Trichoderma citrinoviride strain ITEM 4484 grown in solid-state fermentation on sterile rice kernels. The producing strain was identified at species level by sequence analysis of the internal transcribed spacer regions ITS-1 and ITS-2 of the nuclear...
Article
Puupchenone (1) and a series of its congeners (2-6) have been isolated from a Dysidea sponge. The unprecedented 20-acetoxyhater-unnadienone (2) exhibiting a five-membered contracted ring, has been characterized. In addition, stereochemical assignment of two previously reported acetone adducts of puupehenone (5 and 6) has been made. Finally, the inh...
Article
A new pentasubstituted oxiranyldecene, named viridenepoxydiol, has been isolated (0.9 mg/L) from the culture filtrate of a strain of Trichoderma viride showing in vitro and in vivo antagonistic activity against Sclerotium rolfsii, which is the causal agent of crown and stem rot of artichoke. Viridenepoxydiol was characterized as 3,5,9-trimethyl-2-o...