Dominika Pilat

Dominika Pilat
Aix-Marseille Université | AMU · Centre de Recherche en Neurobiologie-Neurophysiologie de Marseille (UMR 7286 CRN2M)

PhD candidate in Neurosciences

About

15
Publications
2,213
Reads
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309
Citations
Additional affiliations
October 2017 - present
Aix-Marseille Université
Position
  • PhD Student
October 2016 - December 2016
Polish Academy of Sciences
Position
  • Research Assistant
October 2014 - October 2016
Polish Academy of Sciences
Position
  • Master's Student

Publications

Publications (15)
Article
Full-text available
Background Membrane-type matrix metalloproteinase 5 (MT5-MMP) deficiency in the 5xFAD mouse model of Alzheimer's disease (AD) reduces brain neuroinflammation and amyloidosis, and prevents deficits in synaptic activity and cognition in prodromal stages of the disease. In addition, MT5-MMP deficiency prevents interleukin-1 beta (IL-1β)-mediated infla...
Article
We previously discovered the implication of membrane‐type 5‐matrix metalloproteinase (MT5‐MMP) in Alzheimer's disease (AD) pathogenesis. Here, we shed new light on pathogenic mechanisms by which MT5‐MMP controls the processing of amyloid precursor protein (APP) and the fate of amyloid beta peptide (Aβ) as well as its precursor C99, and C83. We foun...
Preprint
Full-text available
We have previously discovered the implication of membrane-type 5-matrix metalloproteinase (MT5-MMP) in AD pathogenesis. Here we shed new light on pathogenic mechanisms by which MT5-MMP controls APP processing and the fate of amyloid beta peptide (Aβ) and its precursor C99. We found that MT5-MMP in HEK carrying the APP Swedish familial mutation (HEK...
Article
Full-text available
Processing of amyloid beta precursor protein (APP) into amyloid-beta peptide (Aβ) by β-secretase and γ-secretase complex is at the heart of the pathogenesis of Alzheimer’s disease (AD). Targeting this proteolytic pathway effectively reduces/prevents pathology and cognitive decline in preclinical experimental models of the disease, but therapeutic s...
Article
Metamizol (also known as dipyrone or sulpyrine) is one of the non-opioid analgesics commonly used in clinical practice in the treatment of somatic and visceral pain. Here, our results give evidence that repeated twice daily intraperitoneal metamizol administration during 7 days diminished development of neuropathic pain symptoms in a mouse model of...
Data
Full-text available
Protein and mRNA levels of TLR2, TLR4 and their adaptor molecules: MyD88 and TRIF are upregulated in spinal cord and/or DRG as measured in three time points during 14-day course of neuropathy development. Pharmacological blockade of Toll-like receptors (TLR2, TLR4) with endogenous antagonists - LPS-RS and LPS-RS Ultrapure, has evoked analgesia in r...
Article
Full-text available
Accumulating evidence indicates that microglial TLR2 and TLR4 play a significant role in nociception. Experiments were conducted to evaluate the contribution of TLR2 and TLR4 and their adaptor molecules to neuropathy and their ability to amplify opioid effectiveness. Behavioral tests (von Frey’s and cold plate) and biochemical (Western blot and qRT...
Article
An interesting research and therapeutic problem is the reduced beneficial efficacy of opioids in the treatment of neuropathic pain. The present study sought to investigate the potential role of IL-1 family members in this phenomenon. We studied the time course of changes in IL-1alpha, IL-1beta, IL-1 receptor type I and IL-1 receptor antagonist mRNA...
Article
Full-text available
Neuropathic pain treatment remains a challenge because pathomechanism is not fully understood. It is believed that glial activation and increased spinal nociceptive factors are crucial for neuropathy. We investigated the effect of parthenolide (PTL) on the chronic constriction injury to the sciatic nerve (CCI)-induced neuropathy in rat. We analyzed...
Data
The graphical abstract of the analgesic effects of parthenolide (PTL) and associated changes in the glial cells, pro- and antinociceptive factors, and correlated signaling pathways activation at day 7 after CCI. Repeated intrathecal administration of PTL in CCI-exposed rats is as follows: (i) decreased neuropathic pain symptoms but enhanced microgl...
Article
Full-text available
The mechanism involved in the development of diabetic neuropathy is complex. Currently, it is thought that chemokines play an important role in this process. The aim of this study was to determine how the level of some chemokines from the CXC subfamily varies in diabetic neuropathy and how the chemokines affect nociceptive transmission. A single in...
Article
Background Neuropathic pain is clinically challenging because it is resistant to alleviation by morphine. The nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathways may be involved in the development of neuropathic pain. The aim of our study was to examine the influence of a chroni...

Questions

Questions (2)
Question
I am doing primary neuronal cultures (mix of neurons and astrocytes) from mice. One year ago we moved and changed labs and since that time I am not able to grow nice neurons (either they don't start growing or die very soon into the differentiation). I did some mycoplasma screening and in some samples I found out the contamination but at a very low level. So here's my question, how fragile neurons are to mycoplasma and also is it possible to make primary culture from animals and avoid mycoplasma contamination at all?
Question
Hi! I have a technical question concerning protein extraction from neuronal primary cultures. When I grow glial cells in the wells (coated with PLL), after I aspirate the supernatant I usually wash them 3 times with clean PBS. It allows me to get rid of all the residual debris and media. After I add RIPA buffer and scrape the cells. With neurons however, I cannot use PBS as a washing solution because neurons don't like salts and when I used it once at the begging, after measuring the protein concentration, it turned out that I barely have any. So right now I am not washing the wells and add RIPA straight away, however I think that the sample/western blot quality is compromised. Do you have experience with that kind of issue?
Thanks

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