Dominik Egger

Dominik Egger
University of Natural Resources and Life Sciences Vienna | boku · Department of Biotechnology

Dr. rer. nat.
Advancing in Cell Culture Technologies to increase therapeutic functionalities of stem cells.

About

39
Publications
21,453
Reads
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303
Citations
Introduction
I am a Senior Scientist and Deputy of the Institute of Cell and Tissue Culture Technologies. The overall aim of my research is to develop new cell culture technologies to improve the therapeutic functionalities of stem cells and stem cell derived extracellular vesicles. For this, I focus on 3D cell culture and bioreactor technologies.
Additional affiliations
October 2017 - present
University of Natural Resources and Life Sciences Vienna
Position
  • PostDoc Position
March 2014 - February 2017
University of Natural Resources and Life Sciences Vienna
Position
  • PhD Student
March 2013 - February 2014
Leibniz Universität Hannover
Position
  • Student Research Assistant
Education
March 2014 - February 2017
October 2011 - February 2014
Leibniz Universität Hannover
Field of study
  • Life Science
October 2008 - September 2011
Leibniz Universität Hannover
Field of study
  • Life Science

Publications

Publications (39)
Article
Full-text available
Mesenchymal stem cells (MSCs) are primary candidates in tissue engineering and stem cell therapies due to their intriguing regenerative and immunomodulatory potential. Their ability to self-assemble into three-dimensional (3D) aggregates further improves some of their therapeutic properties, e.g., differentiation potential, secretion of cytokines,...
Article
Full-text available
The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform comb...
Article
Full-text available
The utilization of mesenchymal stem/stromal cells raises new hopes in treatment of diseases and pathological conditions, while at the same time bringing immense challenges for researchers, manufacturers and physicians. It is essential to consider all steps along the in vitro fabrication of cell‐based products in order to reach efficient and reprodu...
Article
Full-text available
Mesenchymal stem/stromal cells (MSCs) display a variety of therapeutically relevant effects, such as the induction of angiogenesis, particularly under hypoxic conditions. It is generally recognized that MSCs exert their effects by secretion of paracrine factors and by stimulation of host cells. Furthermore, there is increasing evidence that some th...
Article
Full-text available
Background: Mesenchymal stem/stromal cells (MSCs) are considered an important candidate in cell therapy and tissue engineering approaches. The culture of stem cells in a 3D environment is known to better resemble the in vivo situation and to promote therapeutically relevant effects in isolated cells. Therefore, the aim of this study was to develop...
Article
Full-text available
Skin equivalents and skin explants are widely used for dermal penetration studies in the pharmacological development of drugs. Environmental parameters, such as the incubation and culture conditions affect cellular responses and thus the relevance of the experimental outcome. However, available systems such as the Franz diffusion chamber, only meas...
Article
Full-text available
Extracellular vesicles (EVs) are cell-derived membrane structures exerting major effects in physiological as well as pathological processes by functioning as vehicles for the delivery of biomolecules to their target cells. An increasing number of effects previously attributed to cell-based therapies have been recognized to be actually mediated by E...
Preprint
Gangliosides are an indispensable glycolipid class concentrated on cell surfaces with a critical role in stem cell differentiation. Nonetheless, owing to the lack of suitable methods for scalable analysis covering the full scope of ganglioside molecular diversity, their mechanistic properties in signaling and differentiation remain undiscovered to...
Article
Full-text available
As one of the most abundant, multifunctional biological polymers, polysaccharides are considered promising materials to prepare tissue engineering scaffolds. When properly designed, wetted porous scaffolds can have biomechanics similar to living tissue and provide suitable fluid transport, both of which are key features for in vitro and in vivo tis...
Chapter
This chapter explains the importance of 3D cell culture and highlights its potential and benefits in comparison to traditional 2D cultivation. Since 3D cell culture is supposed to mimic the in vivo situation, we will also have a close look on the complex composition of the in vivo microenvironment and why 2D cultivation does not resemble the in viv...
Article
Full-text available
Mesenchymal stem cells (MSCs) are of great interest for their use in cell-based therapies due to their multipotent differentiation and immunomodulatory capacities. In consequence of limited numbers following their isolation from the donor tissue, MSCs require extensive expansion performed in traditional 2D cell culture setups to reach adequate amou...
Book
This textbook shall introduce the students to 3D cell culture approaches and applications. An overview on existing techniques and equipment is provided and insight into various aspects and challenges that researchers need to consider and face during culture of 3D cells is given. The reader will learn the importance of physiological cell, tissue and...
Article
Full-text available
3D printing is increasingly important for the rapid prototyping of advanced and tailor-made cell culture devices. In this context, stereolithography represents a method for the rapid generation of prototypes from photocurable polymers. However, the biocompatibility of commercially available photopolymers is largely unknown. Therefore, we evaluated...
Article
Full-text available
The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. In this work, we used human MSCs, received from surgery waste, and differentiated them into fat...
Preprint
Full-text available
The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. In this work, we used human MSCs, received from surgery waste, and differentiate them into fat a...
Preprint
Full-text available
Mesenchymal stem/stromal cells (MSCs) are considered an important candidate in cell therapy and tissue engineering approaches. The culture of stem cells in a 3D environment is known to better resemble the in vivo situation and to promote therapeutically relevant effects in isolated cells. Therefore, the aim of this study was to develop an approach...
Article
Full-text available
Mesenchymal stem cells (MSCs) are considered as primary candidates for cell-based therapies due to their multiple effects in regenerative medicine. Pre-conditioning of MSCs under physiological conditions—such as hypoxia, three-dimensional environments, and dynamic cultivation—prior to transplantation proved to optimize their therapeutic efficiency....
Poster
Full-text available
Mesenchymal stem cells (MSCs) are widely used in clinical trials for cell based therapies and as the biological part of diverse tissue engineered constructs. Plastic adherence is the oldest method for MSCs isolation and one of the minimal criteria which must be fulfilled for MSCs characterization. However, isolation and expansion of MSCs in non-phy...
Poster
Full-text available
Aggregate cultivation is considered a more physiologic alternative to traditional 2D cell culture. In contrast, the formation of 3D MSC aggregates seems to preserve their phenotype and differentiation potential. Furthermore, increased secretion of proangiogenic factors and anti-inflammatory cytokines was observed after aggregate formation. Mesenchy...
Chapter
A major challenge in tissue engineering and cell-therapy-based approaches is the production of a significant amount of functional cells. Although it does not represent a physiologic environment, most of stem cell cultivation is performed in static conditions (e.g., petri dishes). Furthermore, static expansion or differentiation of stem cells is lab...
Chapter
Traditional two-dimensional (2D) cell culture does not represent the natural environment cells experience in their respective tissue or organ. In contrast, the cultivation of cells in a three-dimensional (3D) environment can help to generate a physiologic environment. This chapter describes the fundamental biological and technological differences o...
Chapter
This chapter gives insight into commercially available automated cultivation systems for cell culture technologies as well as the theoretical background of process automation and modeling. Exemplary bioreactor systems with sensors, actors, and process principles are described, ranging from the classical stirred tank reactor to more advanced perfuse...
Thesis
Full-text available
Mesenchymal stem cells (MSCs) are still considered as the primary candidate for cell-based therapies and tissue engineering products in Regenerative Medicine. They are easily accessible and ethically unobjectionable multipotent stem cells with remarkable properties such as high proliferation and differentiation capacity or the secretion of trophic...
Article
Full-text available
The standard cell culture oxygen concentration of 21 percent O 2 does not represent the physiologic environment of stem cells. Indeed, hypoxic (< 21 percent O 2) conditions heavily affect cellular processes such as growth, apoptosis and energy metabolism. In the context of clinical trials a fast ex vivo expansion of stem cells while maintaining the...
Article
Full-text available
The three dimensional (3D) cultivation of stem cells in dynamic bioreactor systems is essential in the context of regenerative medicine. Still, there is a lack of bioreactor systems that allow the cultivation of multiple independent samples under different conditions while ensuring comprehensive control over the mechanical environment. Therefore, w...
Article
Full-text available
Extensive expansion of mesenchymal stem cells (MSCs) for cell-based therapies remains challenging since long-term cultivation and excessive passaging in two-dimensional conditions result in a loss of essential stem cell properties. Indeed, low survival rate of cells, alteration of surface marker profiles, and reduced differentiation capacity are ob...
Article
It is crucial but challenging to keep physiologic conditions during the cultivation of 3D cell scaffold constructs for the optimization of 3D cell culture processes. Therefore, we demonstrate the benefits of a recently developed miniaturized perfusion bioreactor together with a specialized incubator system that allows for the cultivation of multipl...
Poster
Full-text available
Conventional cell culture procedures are far from representing the environment experienced by mesenchymal stem cells (MSC) in vivo. In order to engineer functional cells and tissue from the scratch one has to consider various/many different parameters to mimic the physiologic environment. For the expansion and guided differentiation of MSC perfusio...
Article
Full-text available
Background and Novelty: The development of stable tissue-engineered autologous bone grafts in the field of regenerative medicine is still a challenge. Perfusion bioreactors not only provide continuous nutrition supply and waste removal, but are also suitable for the controlled application of mechanical forces like fluid shear stress. Mechanical loa...
Article
Full-text available
A main challenge in cell therapies and other tissue regeneration approaches is to produce a therapeutically significant cell number. For expansion of mesenchymal stem cells (MSC) the cultivation on 2D plastic surfaces is still the conventional procedure, even though the culture conditions differ significantly from the 3D environment in vivo. Additi...

Questions

Questions (6)
Question
I am looking for human BMP7 that can be used as a cell culture supplement for differentiation. There are many vendors with extremely different prices. I.e. at abcam I can find three BPM7 products:
Can you recommend a specific vendort or product?
Thank you.!
Question
I want to stain the fibrin network of a hydrogel constituted of fibrin for confocal microscopy. However, I did not find dye that would directly stain for fibrin. Is there any? If not, does anyone have experience in staining fibrin (i.e. can you recommend any secondary antibody system)?
Thanks for your replies!
Question
Can anyone suggest a good method / composition of enzymes that allow for an efficient singularization / separation of cells from a mesenchymal stem cell aggregate / spheroid?
I tried to detach cells from one another in order to receive a single cell suspensions after 3D culitvation in an aggregate / spheroid with: collagenase (tried severalconcentrations), Accutase, Accumax, Trypsin, TrypLE, Elastase, Dispase and mixtures of these. None of it worked very well. Recovery is max. 50 %.
I am working with primary MSCs only and they produce a massive amount of ECM or at least cell cell connections. Its crazy... I know from other kinds of stem cells or stem cell cell lines that usually enzymes like Accumax / Accutase / Trypsin or collegenase will do the trick. But I did not have success with them on MSCs.
Any suggestions?
Thanks a lot.
Question
Hi, I want to use elastase to optimize the dissociation of cells from spheroids. Currently I am screening different enzymes and combinations of enzymes for that purpose.
However, for elastase I found very little literature on what concentration (mg/ml) is usually used during the dissociation of different tissues.
It would be even better to state the activity (U/ml) but this is even more rare.
If you are familiar with the use of elastase I would also appreciate information on how to prepare a proper stock solution (concentration, buffer, pH ...) since there is also differing information on that on the web.
Thanks a lot!

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Cited By

Projects

Projects (4)
Project
Time resolved non-destructive monitoring and sampling from within 3D cell- and tissue-based models (4D applications).
Project
In this project, we are working on new methods for the isolation of mesenchymal stem cells. Usually, these cells grow on two-dimensional plastic surfaces which is far away from their physiologic environment. Therefore, we work on methods for the direct isolation of MSCs into a three-dimensional environment. Our final goal to enable 3D cultivation in all phases of in vitro cultivation of MSCs, , avoiding any contact to a two-dimensional plastic surface. Using 3D isolated cells will probably also increase the physiologic relevance of in vitro models.
Project
Aggregate cultivation is more and more considered as a more physiologic way to cultivate MSCs. Therefore, we want to develop a bioprocess for the expansion of MSCs in aggregates.