Research Items (8)
Changes in epigenetic polymorphism degree within wheat seedlings’ population under radiation stimulating exposure of germination were investigated. Variations in seeds sample allocations by germination rate in both control and chronically exposed variants were estimated. Changes in DNA methylation patterns of seedlings from seeds with different germination rate were studied. Variations in epigenetic polymorphism–“distance” between DNA methylation patterns of “fast” and “slow” seedlings of different wheat varieties under radiation exposure were assessed. Indicated, that increased germination rate of seeds sample was associated with decreasing degree of epigenetic polymorphism. Issue concerning both role of epigenetic polymorphism in plant population stability and its decreasing degree that could initiate less effectiveness of production process was discussed.
The correlation between the ecological plasticity of the eight premium winter wheat varieties and the DNA methylation pattern polymorphism within variety has been studied using the seedlings from seeds with different germination rate. Polymorphism degree or “epigenetic distance” in restriction fragment range of PCR products has been assessed using Nei index. The correlation between variety grade determined by its productivity and ecological plasticity and the degree of DNA methylation pattern polymorphism within variety (Rs=0.69) has been found. The prevalence of the greatest “epigenetic distance” (D≥0.1) in the most productive and ecologically plasticized varieties has been proved. The “epigenetic distance” assessment has been recommended to apply to the selection of new varieties for the areas with unstable climate conditions.
Досліджено зв’язок екологічної пластичності восьми елітних сортів озимої пшениці з поліморфізмом профілів метилування ДНК в межах сорту у проростків з насіння, що проростає з різною швидкістю. Рівень поліморфізму, або «епігенетичну відстань», у спектрах рестрикційних фрагментів електрофореграм розподілу продуктів полімеразної ланцюгової реакції (ПЛР) кількісно оцінювали за показником Неї. Встановлено існування кореляції (Rs = 0,69) між рангом, що визначається по продуктивності та екологічній пластичності, і рівнем поліморфізму профілів метилування ДНК сорту. Показано, що найбільша «епігенетична відстань» (D ≥ 0,1) спостерігається у сортів з найвищим рангом по продуктивності та екологічній пластичності. Запропоновано використовувати оцінку показника епігенетичної відстані у селекції нових сортів для зон з нестійкими кліматичними умовами.
- Dec 2014
Interaction between changes of functionally different DNA methylation pattern and chromosomal aberration yield under fractionated UV-C exposure at the doses-of 6.2-9 kJ/m2 have been investigated. Two ways of meristematic tissue renewal after irradiation have been revealed on the grounds of statistical analysis. To estimate changes in the methylation pattern of transcribed and satellite DNA the restriction analysis was carried out. The restriction reaction was followed by PCR with ITS1(19b), ITS4(20b) and ISSR(14b) primers. The obtained data indicate specific changes in the methylation pattern of transcribed and satellite DNA parts under fractionated irradiation with different fraction ranges. Possible role of these changes in the progressing irradiation damage and inducible protection reactions is discussed.
- Jul 2014
The hypothesis on the connection between various germination rates of seeds that are not in dormancy with their epigenetic polymorphism has been checked. It was shown that seedlings that were characterized with different germination rates had different methylation patterns of transcribed and satellite DNA. Different cytogenetic characteristics and their changes were observed under UV-C irradiation as well.
- Feb 2013
Relationship of changes of methylation pattern of functionally different parts of DNA and chromosomal aberration yield was studied at the conditions of the fractionating of UV-C irradiation. Combination of restriction analysis (Hpall, MspI, MboI enzymes) with the subsequent raising of PCR (internal transcribed space ITS1, 1TS4 and inter simple sequence repeat - ISSR, 14b primers) was used. The got results testify to the changes in methylation pattern of satellite and transcription active part of DNA atan irradiation in the mode of fractionating and depending on fraction time ranges. The role of the methylation DNA pattern change in development of radiation damage and induction of organism protective reactions was discussed.
The relationship between changes in the methylation pattern of functionally different parts of DNA and the chromosomal aberration’s yield was studied under the conditions of UV-C irradiation fractionating. A combination of restriction analysis (HpaII, MspI, and MboI enzymes) with subsequent PCR (internal transcribed spacer ITS1 and ITS4 and inter simple sequence repeat ISSR(14b) primers) was used. The obtained results showed changes in the methylation pattern of the satellite and transcribed DNA part of DNA at irradiation in the fractionating mode, depending on the fraction range. The role of a DNA methylation pattern changes in the development of radiation damage and induction of the organism’ protective reactions was discussed.
The differences in satellite DNA methylation pattern of corn seedlings with various spontaneous chromosome aberration yields and changes in methylation pattern of these DNA sequences under different exposure modes of acute UV-C and chronic gamma-irradiations have been investigated. The obtained experimental data and the conducted correlation analysis demonstrated the significant correlation between the satellite DNA methylation pattern varieties and chromosome aberration yields under various stress exposure modes. The role of satellite DNA methylation pattern variability and its changing in key responses to stress such as mobile elements’ activation, cell’s passage of checkpoints, and homological repair was discussed.
All co-authors (9)