
Dmitriy Bazhenov
Dmitriy Bazhenov
Looking for collaborations, insights, and technics (good science memes are also appreciated, I guess :)
About
20
Publications
2,520
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72
Citations
Introduction
Education
September 2016 - June 2018
September 2012 - June 2016
Publications
Publications (20)
Natural killer cells (NK cells) exert cytotoxicity towards target cells in several ways, including the expression of apoptosis-mediating ligands (TRAIL, FasL). In addition, NK cells themselves may be susceptible to apoptosis due to the expression of TRAIL receptors. These receptors include TRAIL-R1 (DR4), TRAIL-R2 (DR5), capable of inducing apoptos...
The interaction of natural killer and trophoblast cells underlies maternal-fetal immune tolerance. The data on the participation of endoglin (ENG, CD105), or its soluble form, in the regulation of the communication of these cells are currently insufficient. In this study, we have investigated the role of endoglin in the intercellular interactions b...
Natural killer cells (NK cells) represent a group of lymphocytes of innate immunity. In addition to NK cells of peripheral blood, tissue-resident populations are described. NK cells of the decidual envelope (decidual NK cells) represent one of the local NK cell populations. Decidual NK cells differ in phenotype and function from peripheral blood NK...
Иммуноглобулины для внутривенного введения (ВВИГ) применяют в терапии бесплодия. Предполагается влияние ВВИГ на цитотоксическую функцию NK-клеток, однако механизм действия ВВИГ остается неясным. Цель: Оценка влияния препарата ВВИГ на цитотоксичность NK-клеток в отношении клеток-мишеней линии К-562 и клеток трофобласта линии JEG-3. Материалы и метод...
We analyzed the effect of individual cytokines that are secretory products of placenta typical of the uteroplacental bed. The proinflammatory cytokines IL-6, IFNγ, and IL-1β increased the expression of TGFβR2 molecule by trophoblast cells, while VEGF and PLGF increased the expression of CD45, CD29, and CD54 adhesion molecule by trophoblast cells. T...
Objective
The present study was to estimate the role of cytokines for trophoblast death in NK cells presence.
Methods
This study involves assessment of NK-92 line NK cell cytotoxic activity against JEG-3 line cells, in presence of cytokines. We also assessed the effect of secretory placenta products on NK cell cytotoxic activity toward JEG-3 line...
Background
Maternal natural killer cells (NK cells) are a prevailing leukocyte population in the uteroplacental bed. The contact interaction and/or cytokine production of these cells controls trophoblast differentiation, migration and invasion.
Objective
To assess the impact of supernatants obtained during culturing of placentas from the first and...
Natural killer (NK) cells are the main population of leukocytes in decidua during the first trimester of pregnancy. NK cells can have contact with trophoblast cells during pregnancy, which raises the possibility of mutual influence. This research aimed to evaluate the proliferation and phenotype of peripheral blood NK cells in the presence of troph...
The aim of this research was to assess the proliferative activity of Natural Killer Cells (NK cells) from Peripheral Blood Mononuclear Cells (PBMCs) in the presence of trophoblast cells in women with a history of recurrent miscarriages. We examined the peripheral blood of women with recurrent miscarriage in the proliferative (n = 12) or secretory (...
The trial objective was to determine the peripheral blood NK cells cytotoxic activity effect on trophoblast cells at recurrent pregnancy loss (RPL). The investigation involved non-pregnant women with PRL in proliferating and secretory menstrual cycle phases (PMCPh and SMCPh, respectively); women of 6–7 weeks pregnancy with RPL in past medical histo...
Preeclampsia still remains one of the most severe pregnancy complications and is an actual problem in the obstetrics practice. At present, the joint impact of cytokines and other placenta secreted factors on trophoblast cell functional activity during preeclampsia complicated pregnancy remains unclear. The aim of the study is to estimate the surfac...
We evaluated cytotoxic activity of peripheral blood NK cells towards trophoblast cells. NK cells either isolated or in the composition of mononuclear cell fraction, caused death of trophoblast cells. In women with physiological pregnancy, the cytotoxic effect of NK cells present in mononuclear cell fraction preincubated with IL-2 was lower than in...
All lymphoid cells are referred to as an innate or adaptive immunity unit in terms of the mechanisms of performing immune reactions. The functional activity of natural killer (NK) cells is not associated with pre-activation processes resulting from contact with antigen, rearrangement of antigen-recognition receptor genes, and clonal proliferation....
At the present time, a broad spectrum of CD8+ T lymphocyte subsets is revealed, including naïve cells, memory cells and regulatory subpopulations. Along with cells with high cytolytic activity, some subsets with marked regulatory activity were found there. Each subpopulation is characterized by a set of produced mediators, surface and intracellular...
Cytotoxic activity is one of the most important way of cellular communication. When one cell population eliminates another, it isn’t always associated with the pathology or defense. The cooperation between mother’s immune cells and fetal cells is a great example of such ‘cytotoxic communication’. Natural killer cells (NK cells) and trophoblast cell...
Introduction: Pregnancy is associated with alterations in leucocytes functional characteristics. The aim was to assess functional activity of NK cells at healthy pregnancy and preeclampsia.
Materials and methods: The groups included nonpregnant women, healthy pregnant women, pregnant women with preeclampsia. For assessment of NK cells cytotoxic act...
NK cells present in different organs differ by their functional characteristics, in particular, proliferative activity. For studying tissue-resident NK cells, tissue-specific microenvironment should be reproduced. In case of decidual NK cells, this microenvironment is created by trophoblast cells. We developed a method for evaluation of proliferati...
Placenta organization significantly depends on trophoblast cells functional activity. Pregnancy complications like preeclampsia are accompanied by trophoblast cells proliferation failure. The goal was to study influences of the drugs “Cytoflavin” and “Magnegamma” to proliferation tropblast cells. It is established that the drugs “Cytoflavin and Meg...
Questions
Question (1)
Dear colleagues, here is the problem:
- got to detect pSTAT-1 in THP-1 cell line
Looking for advice:
- what positive control it is better to use?
- what is/are the best timepoint/timepoints ?
- what is the best kit to use for fixation/permeabilization/intracelluler staining(for flow cytometry)?
- any piece of advise or protocol will be usefull)