Dirk Schaumlöffel

Dirk Schaumlöffel
Université de Pau et des Pays de l'Adour | UPPA · Institute of Interdisciplinary Research on Environment and Materials (IPREM)

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91
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Introduction
Skills and Expertise

Publications

Publications (91)
Article
The bioavailability of trace elements in the course of evolution had an essential influence on the emergence of life itself. This is reflected in the co-evolution between eukaryotes and prokaryotes. In this study, the influence and cellular distribution of bioelements during phagocytosis at the host-pathogen interface was investigated using high-re...
Article
Purpose: Several preclinical studies have reported the presence of gadolinium (Gd) in different chemical forms in the brain, depending on the class (macrocyclic versus linear) of Gd-based contrast agent (GBCA) administered. The aim of this study was to identify, with a special focus on insoluble species, the speciation of Gd retained in the deep c...
Article
Full-text available
As an essential trace element, copper plays a pivotal role in physiological body functions. In fact, dysregulated copper homeostasis has been clearly linked to neurological disorders including Wilson and Alzheimer’s disease. Such neurodegenerative diseases are associated with progressive loss of neurons and thus impaired brain functions. However, t...
Article
Mixotrophic micro‐organisms are able to use organic as well as inorganic carbon sources and, thus, play an essential role in the biogeochemical carbon cycle. In aquatic ecosystems, the alteration of CO2 fixation by toxic metals such as cadmium ‐ classified as a priority pollutant ‐ could contribute to the unbalance of the carbon cycle. In consequen...
Article
Full-text available
Nanospheres of lead (Pb) have recently been identified in zircon (ZrSiO4) with the potential to compromise the veracity of U-Pb age determinations. The key assumption that the determined age is robust against the effects of Pb mobility, as long as Pb is not lost from the zircon during subsequent geological events, is now in question. To determine t...
Article
Brown seaweed such as Laminaria digitata is known to accumulate arsenic to more than 100 mg/kg. How the algae can tolerate such high level of arsenic has traditionally been studied by arsenic speciation analysis using HPLC-ICPMS, but the knowledge of its molecular forms has not yet given any answers. Here we use HPLC-ICPMS/MS coupled simultaneously...
Article
Glutathione transferases (GSTs) represent a widespread enzyme superfamily in eukaryotes and prokaryotes catalyzing different reactions with endogenous and xenobiotic substrates such as organic pollutants. The latter are often found together with metal contamination in the environment. Besides performing of essential functions, GSTs protect cells by...
Article
Full-text available
A careful separation, identification and characterization of polymer-coated quantum dots (P-QDs) in complex media such as soil solution is the key point to understand their behaviour and to accurately predict their fate in the environment. In the present study, a synthesized CdSe/ZnS core/shell P-QDs suspension, proved to be stable for at least six...
Article
Aquatic fungi can be used to evaluate the functioning of natural ecosystems. Heliscus lugdunensis is an early colonizer of allochthone leafs. Since this aquatic hyphomycete is able to develop in metal contaminated habitats and tolerates cadmium, it appears to be a good candidate to investigate adaptation to metal pollution. This study aimed at exam...
Article
Full-text available
Emergent Contaminants (ECs) including nanoparticles (NPs) are preoccupation cause for concern for the scientific community, industrials, territorial communities and the general public. High production volume, potential release from consumer product, environmental exposure, we need to measure the environmental concentrations of NPs and their behavio...
Article
The green micro-alga Chlamydomonas reinhardtii is commonly used as a model to investigate metallic stress in photosynthetic organisms. The aim of this study was to explore processes implemented by three C. reinhardtii strains to cope with cadmium (Cd), and particularly to evidence Cd sequestration in the cell. For that, we used a combination of sub...
Article
Fluctuations in concentrations of bioavailable metals occur in most natural waters. In situ measurements are desirable to predict risks of adverse effects to aquatic organisms. We evaluated Diffusive Milli-Gels (DMG), a new in situ passive sampler, for assessing the bioavailability and toxicity of copper in waters exhibiting a wide range of charact...
Chapter
Electron microscopy (EM) and nano secondary ion mass spectrometry (NanoSIMS) aim to acquiring nanometric information, which also imply ultraresolution and therefore these techniques require the best preservation of samples. Analytical techniques such as X-ray spectroscopy and NanoSIMS are able to identify, localize and quantify chemical elements bo...
Chapter
This chapter presents four complementary element-specific bioimaging techniques: laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), nano secondary ion mass spectrometry (NanoSIMS), transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy (TEM/X-EDS), and synchrotron-based X-ray fluorescence (SXRF). LA...
Chapter
The metallome is defined as the entirety of metal and metalloid compounds in cells, body fluids, or tissues of an organism and results from the interactions and functional connections between metal ions and DNA, RNA, proteins, and metabolites. Thus, the metallome can be regarded as a subcategory of the genome, transcriptome, proteome, and metabolom...
Article
Full-text available
ᅟThe term speciation is used for over 30 years, with different meanings. In the early 2000s, a reference definition was proposed. The elemental distribution is discussed only with respect to molecular structures. Polytatomic entities such as amorphous or crystalline structures, dispersed in a liquid or gas, or in the solid phase, are not taken into...
Article
Full-text available
An important application field of secondary ion mass spectrometry at the nanometer scale (NanoSIMS) is the detection of chemical elements and, in particular, metals at subcellular level in biological samples. The detection of many trace metals requires an oxygen primary ion source to allow the generation of positive secondary ions with high yield i...
Article
Chemical bioimaging offers an important contribution to the investigation of biochemical functions, biosorption and bioaccumulation processes of trace elements via their localization at the cellular and even at the subcellular level. This paper describes the combined use of high contrast transmission electron microscopy (HC-TEM), energy dispersive...
Article
A new passive sampler was designed and characterized for the determination of free copper ion (Cu(2+)) concentration in aqueous solution. Each sampling device was composed of a set of about 30 diffusive milligel (DMG) beads. Milligel beads with incorporated cation exchange resin (Chelex) particles were synthetized using an adapted droplet-based mil...
Chapter
The-omics tool box refers to a set of modern analytical techniques developed during the past decades that paved way for a remarkable progress of knowledge in biology, biochemistry, and life sciences. The aim of these new technologies is to acquire - ideally - complete sets of molecular data of the genome, proteome, transcriptome, metabolome, and re...
Book
DOI: 10.1002/9783527686063.ch18
Article
Fish bile metallothioneins (MT) have been recently reported as biomarkers for environmental metal contamination; however, no studies regarding standardizations for their purification are available. Therefore, different procedures (varying centrifugation times and heat-treatment temperatures) and reducing agents (DTT, β-mercaptoethanol and TCEP) wer...
Article
Quantitative mass spectrometry is a powerful tool for the determination of enzyme activities as it does not require labeled substrates and simultaneously allows for the identification of reaction products. However, major restrictions are the limit number of samples which can be measured in parallel due to the need for isotope labeled internal stand...
Article
Low background signals are an indispensable prerequisite for accurate quantification in bioanalytics. This poses a special challenge when using derivatized samples, where excess reagent concentrations are increasing the background signal. Precleaning steps often are time consuming and usually lead to analyte losses. In this study, a set of labeled...
Article
Full-text available
Author Summary Schistosomiasis is a chronic, debilitating disease that affects hundreds of millions of people. The treatment of schistosomiasis relies solely on monotherapy with praziquantel and there is concern that drug-resistant parasites will evolve. Therefore, it is imperative to identify new drugs for schistosomiasis treatment. In this study...
Article
Two peptide quantification strategies, the isobaric tags for relative or absolute quantitation (iTRAQ) labeling methodology and a metal-chelate labeling approach, were compared using matrix-assisted laser desorption/ionization-TOF/TOF MS and MS/MS analysis. Amino- and cysteine-directed labeling using the rare earth metal chelator 1,4,7,10-tetraazac...
Article
Full-text available
Dieser Review-Artikel gibt einen Überblick über die Analyse anorganischer Nickelspezies und ihre toxischen Effekte. Auf der Grundlage des eingesetzten analytischen Verfahrens werden anorganische Nickelspezies üblicherweise in lösliche, sulfidische, metallische und oxidische Fraktionen eingeteilt. Nur in wenigen Arbeiten wurde versucht, die verschie...
Article
Aquatic environments like peat bogs are affected by anthropogenic metal input into the environment. These ecosystems are inhabited by unicellular green algae of the class Zygnematophyceae. In this study the desmid Micrasterias denticulata was stressed with 600nM Cd, 10μM Cr and 300nM Cu for 3weeks. GSH levels were measured with HPLC and did not dif...
Article
This review gives an overview on the analysis of inorganic nickel species and their toxic effects. Based on the analytical procedure applied inorganic nickel species are usually classified in soluble, sulfidic, metallic and oxidic nickel fractions. Only few works were attempting a chemical characterization of the different nickel compounds in each...
Article
This review gives an overview and critical evaluation about modern LC-MS techniques for identification and quantification of thiol peptides and their metal complexes in seed plants, algae and fungi. In analogy to other research fields we propose the term metallo-thiolomics for the global study of the entirety of thiol peptides and their metal compl...
Article
Chlamydomonas reinhardtii is a common model organism for investigation of metal stress. This green alga produces phytochelatins in the presence of metal ions. The influence of cadmium is of main interest, because it is a strong activator of phytochelatin synthase. Cell wall bound and intracellular cadmium content was determined after exposition to...
Article
A brief survey is given of the last 2 years’ literature on electrospray mass spectrometry (ESI-MS) for speciation analysis. As observed for many years, the main recent applications in this field concern arsenic and selenium species, especially in studies encompassing combined use of molecular and element mass spectrometry. A further application fie...
Article
Metal labelling of peptides and proteins using high-affinity metal-chelating compounds has found widespread applications in the medical and bioanalytical fields. In the present study we investigated the analysis of peptides derivatized either with cysteine- or amino group-directed metal-bound DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraaceti...
Article
A novel nanonebulizer (nDS-200e) with extended liquid flow rate range was developed for ICP-MS sample introduction from 50 to 4000 nL min−1. The new device has a distinctly improved design compared to the first prototype (P. Giusti, R. Lobinski, J. Szpunar and D. Schaumlöffel, Anal. Chem., 2006, 78, 965).1 The robust construction using a very thin...
Article
Quantitative phytochelatin (PC) analysis is, due to oxidation sensitivity of the PCs, matrix effects, and time consuming sample preparation, still a challenging analytical task. In this study, a rapid, simple, and sensitive method for precise determination of native PCs in crude extracts of the green alga Chlamydomonas reinhardtii was developed. Al...
Article
Method development for the analytics of biomolecules has an enormous impact on biochemistry, biology, medicine and life sciences. In particular, methods for peptide and protein quantification are required for the study of the proteome of organisms in order to understand fundamental processes of life. Most popular approaches use stable isotope label...
Article
An Ussing chamber study was conducted in order to investigate the transport behavior of copper glycinate complexes through a porcine gastrointestinal membrane. Organic copper complexes such as copper tri- and tetraglycinates (GGG-Cu(II) and GGGG-Cu(II)) were used as model system. In a novel analytical approach the Ussing chamber was combined with m...
Article
Phytochelatins (PC) were described earlier to play a role in metal detoxification in Chlamydomonas reinhardtii but were not clearly identified. The focus of this case study was to identify PC synthesized by C. reinhardtii exposed to Cd. Only low intracellular concentrations of cadmium (85 nmol g−1 fresh weight) were sufficient to cause significant...
Article
The need of analytical methods for absolute quantitative protein analysis spurred research on new developments in recent years. In this work, a novel approach was developed for accurate absolute peptide quantification based on metal labeling with lutetium diethylenetriamine pentaacetic acid (Lu-DTPA) and nanoflow high-performance liquid chromatogra...
Article
An approach based on the consecutive use of nanoHPLC-ICP collision cell MS and nanoHPLC-electrospray MS was proposed for the analysis of water-soluble selenium-containing proteins in selenium-rich yeast after their separation by 2D gel electrophoresis (GE). An ultrasonic probe was employed for fast protein extraction avoiding sample heating and thu...
Article
An ICP-MS-assisted proteomics approach was developed for the characterization of Se-containing proteins in selenium-rich yeast. Ultrasound probeleaching of a yeast sample with 8 M urea and 4% CHAPS and purification were optimized for highest selenium and proteinextraction yield. The proteins were separated by 2D gel electrophoresis. The spots conta...
Article
The element sulfur is almost omnipresent in all natural proteomes and plays a key role in protein quantification. Incorporated in the amino acids cysteine and methionine, it has been served as target for many protein-labeling reactions in classic quantitative proteomic approaches based on electrospray or MALDI mass spectrometry. This critical revie...
Article
A novel generic approach based on precolumn isotope dilution nanoHPLC-ICPMS analysis was developed for the accurate absolute quantification of sulfur-containing peptides. A 34S-labeled, species-unspecific sulfur spike (sulfate), noninteracting with analyte peptides under the optimized HPLC condition, was added directly to the chromatographic eluent...
Article
Full-text available
A method for accurate selenium determination in blood serum by isotope dilution analysis (IDA) was developed. Selenium was determined by ICP octopole reaction system MS using xenon as the collision gas (130-160 mL min 1 ) in order to eliminate polyatomic interferences from argon, chlorine, phosphorus and bromine. The detection limit was 3.3 m gL 1...
Article
The potential of inductively coupled plasma-mass spectrometry (ICP-MS), which allows element-specific detection of heteroelements (e.g. Se and S) incorporated in protein structures, is highlighted for sensitive qualitative and quantitative protein analysis. ICP-MS coupled to separation techniques such as size exclusion chromatography and gel electr...
Article
Full-text available
An interface based on a total consumption micronebulizer was developed for the introduction of xylene solutions into ICP MS. The increase in the nebulizer capillary diameter and the elimination of the internal connections reduced the problem of clogging, pressure instability and memory effects. The xylene carrier could be introduced for several hou...
Article
ICP collision cell MS was optimized for the detection and retention-time marking of selenium-containing peptides in nanoHPLC (75 μm column) after on-line 100-fold preconcentration on a capillary (300 μm id) precolumn. The mobile phase composition, gradient and flow rate were chosen to allow electrospray-MS/MS to be successfully run in parallel in i...
Article
A high-throughput flow-injection — inductively coupled plasma mass spectrometry (ICP MS) analytical method was developed for the determination of mercury in gas condensates and carbon-rich solvents. The sample (undiluted or diluted 10-fold) was introduced via a modified total consumption micronebulizer working at a flow rate of 30 μl min−1 and fitt...
Article
A method based on ICP collision-cell MS detection in capillary HPLC was developed to gain an insight into the purity and identity of selenium-containing proteins separated by 1-D and 2-D electrophoresis. The bands and spots obtained after the separation of water-soluble proteins in selenized yeast were digested with trypsin prior to chromatography....
Article
Immunoaffinity chromatography has been investigated for fractionation of serum into selenoalbumin and true selenoproteins. Among several albumin-depletion kits tested, a multiaffinity column specifically binding albumin and five other major serum proteins provided the best results. It extracted ca 95% of both albumin and selenoalbumin, which enable...
Article
A novel nebulizer (nDS-200) working at sample uptake rates of less than 500 nL min(-1) was developed for a sheathless interfacing of nanoHPLC (75-microm column i.d.) with ICPMS. It was based on a hollow fused-silica needle of which the tip (i.d. 10 microm, o.d. 20 microm) centered in a 254-microm-i.d. sapphire orifice. The nebulizer, equipped with...
Article
A considerable momentum has recently been gained by in vitro and in vivo studies of interactions of trace elements in biomolecules due to advances in inductively coupled plasma mass spectrometry (ICP MS) used as a detector in chromatography and capillary and planar electrophoresis. The multi-isotopic (including non-metals such as S, P, or Se) detec...
Article
Laser ablation ICP-MS (inductively coupled plasma mass spectrometry) is becoming one of the most important analytical techniques for fast determination of trace impurities in solid samples. Quantification of analytical results requires matrix-matched standards, which are in some cases (e.g., high-purity metals, proteins separated by 2D gel electrop...
Patent
The nebulizer with a nanometric flow rate of a liq. effluent in a nebulizing gas comprises at least, arranged substantially concentrically, a capillary tube for intake of the liq. effluent and a nebulizing needle including a central channel fed with liq. effluent through the capillary tube, a chamber for intake of the nebulizing gas feeding a nozzl...
Article
Full-text available
A sensitive analytical procedure based on nano-volume flow injection (FI) and inductively coupled plasma double-focusing sector field mass spectrometry (ICP-SFMS) was developed for the ultratrace determination of uranium and plutonium. A 54-nl sample was injected by means of a nanovolume injector into a continuous flow of carrier liquid at 7 mul mi...
Chapter
IntroductionNickel Speciation in the EnvironmentNickel Speciation in FoodClinical and Occupational Health Aspects of Nickel SpeciationConclusions References
Article
An interface between nanoHPLC and ICP-MS was developed. It allowed the stable introduction into an ICP of mobile phases containing up to 90% of acetonitrile at flow rates of less than 500 nL min � 1 . The on-line post-column addition of an isotopically enriched spike at flow rates of less than 4 mL min � 1 enabled isotope dilution quantification of...
Article
The recent developments and trends in quantitative speciation analysis of endogenous trace element compounds in biological systems by ICP isotope dilution MS are discussed. The use of two different isotope dilution techniques is critically evaluated with respect to their advantages and limitations: species-unspecific isotope dilution for the quanti...
Article
A method based on the species-specific isotope dilution analysis was developed for the accurate determination of an Asp-Tyr-SeMet-Gly-Ala-Ala-Lys peptide directly in a tryptic digest of an aqueous extract of selenized yeast. For this purpose a 77Se labeled peptide to be used as a standard had been purified by 2D liquid chromatography from yeast gro...
Article
A method for the accurate determination of selenoamino acids in human serum by HPLC-ICPMS was developed using the species-specific isotope dilution analysis principle. A serum sample was enzymatically digested with a mixture of lipase and protease after derivatization of the selenocysteine residues with iodoacetamide. The selenoamino acid fraction...
Article
Different techniques for capillary liquid separation with ICP MS detection are described. high-performance liquid chromatography (HPCL) is a robust, reliable and reproducible separation technique for various types of samples that provides high resolution, and can be easily implemented for various samples and application areas. HPCL can be scaled do...
Article
Full-text available
First published as an Advance Article on the web 17th May 2004 Speciation of thallium was investigated in a Tl hyperaccumulator plant, Iberis intermedia, by ion chromatography (IC) and size-exclusion chromatography (SEC) coupled with on-line ICP-MS detection. The leaves, stems and roots of the plant were extracted with a buffer solution (pH 6.2) co...
Chapter
Interactions of plants with metals and metalloids have attracted considerable attention in recent years (Merian 1991; Prasad 1996). This interest has been for a number of different reasons, reflecting the variety of areas of the importance of plants and the different roles played by the different elements. Indeed, whereas many elements (e.g. B, Se)...
Article
A sheathless interface based on a total consumption micronebulizer operating at flow rates in the range 0.5-7.5 microL min(-1) was developed between capillary HPLC and ICPMS. It allowed the efficient nebulization and transport into the plasma of mobile phases containing up to 100% organic solvent without either cooling the spray chamber or oxygen a...
Article
Species analysis of metallothionein-like proteins (MLP) in liver tissues from Elbe-Bream (Abramis brama L.) and Roe Deer (Capreolus capreolus L.) using capillary electrophoresis (CE) combined with inductively coupled plasma mass spectrometry detection is described. In order to allow systematic development of the method, commercially available metal...
Article
Capillary zone electrophoresis, with its high resolution capability in the separation of different compounds, is well suited for the investigation of metal-containing proteins, especially when elemental detection is conducted using hyphenated inductively coupled plasma-mass spectrometry. A major problem in the separation of proteins in body fluids...
Article
An on-line isotope dilution (ID) method in combination with the coupling of size-exclusion high-performance liquid chromatography (SE-HPLC) to an axial inductively coupled plasma time-of-flight mass spectrometer (ICP-TOFMS) was applied to the quantitative speciation of Cu, Zn and Cd in carp and eel cytosols. Quantitative information on the distribu...
Article
A novel analytical approach based on a combination of multidimensional hyphenated techniques and cloning of the Ni-resistance gene using yeast complementation screens was developed for the identification of nickel species in a Thlaspi caerulescens hyperaccumulating plant. The presence of an unknown strong Ni complex was demonstrated by size exclusi...
Article
Speciation of nickel was investigated in a hyperaccumulator tree, Sebertia acuminata, by liquid chromatography (LC) with parallel ICP MS and electrospray MS-MS detection. The latex of the tree was leached with water. Two separation mechanisms, size-exclusion (SEC) and capillary zone electrophoresis (CZE), were investigated for the separation of Ni-...
Article
The complementarity of ICP sector-field double-focussing (SF-DF) MS and electrospray MS detection in capillary zone electrophoresis (CZE) was studied for the identification and determination of metal complexes with metallothionein (MT) induced in the liver of a rat exposed to intravenously administered Cd2+. The MT fraction investigated was isolate...
Article
Recent developments in the coupling of highly selective separation techniques such as capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) to element-specific and molecule-specific detectors, such as inductively-coupled plasma mass spectrometry (ICP-MS) and electrospray ionization-tandem mass spectrometry (ESI-MS/MS) for...