Dirk Janssen

Dirk Janssen
Instituto de Investigación y Formación Agraria y Pesquera | IFAPA · Centro La Mojonera

PhD

About

135
Publications
17,362
Reads
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1,200
Citations
Additional affiliations
January 1997 - present
Institute of Research and Training in Agriculture and Fisheries of Andalousia (IFAPA)
Position
  • Institute of Agricultural Research and Training (IFAPA)
January 1994 - December 1995
Universidad de Granada

Publications

Publications (135)
Article
Symptom expression and levels of the ipomovirus Cucumber vein yellowing virus (CVYV) and the crinivirus Cucurbit yellow stunting disorder virus (CYSDV) were compared in greenhouse cucumbers in single and mixed infections. Results were contrasted with those obtained for plants infected with the potyvirus Zucchini yellow mosaic virus (ZYMV) in single...
Preprint
Full-text available
The recurrent emergence of virus diseases in intensive horticultural crops requires alternative control strategies. Topical application of dsRNA molecules homologous to pathogens has been proposed as a tool for plant virus control. These dsRNAs induce the silencing mechanism (RNAi) that degrades homologous dsRNAs. Cucumber green mottle mosaic virus...
Article
Full-text available
The tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite, single-stranded begomovirus that was first identified in India in 1995 affecting solanaceous crops. A different strain, named ToLCNDV-ES, was introduced in Spain in 2012 and causes severe symptoms in zucchini crops. Virus transmission experiments with the whitefly Bemisia tabaci, were u...
Article
Dragon fruit (Hylocereus undatus) is a high-value fruit crop, introduced about a decade ago in the mainland of Spain. In 2021, chlorotic spots were observed on young cladodes in a commercial dragon fruit orchard in the province of Seville (southern Spain). Sap extracts from 4 symptomatic cladodes were used to mechanically inoculate indicator plants...
Article
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We are pleased to present this Special Issue on the topic of “Plant virus Epidemiology” [...]
Article
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Cucumber green mottle mosaic virus (CGMMV) is a severe threat to melon production worldwide. At present, there are no cultivars available on the market which show an effective resistance or tolerance to CGMMV infection; only wild Cucumis species were reported as resistant. Germplasm accessions of Cucumis melo, as well as C. anguria, C. ficifolius,...
Article
Full-text available
Generalist and specialist predators are successfully used in biocontrol programs in greenhouse vegetable crops, like tomato. A greenhouse ecosystem is rather simple and provides an excellent opportunity for developing predator–prey decision models. Three systems were selected: (1) the generalist predatory bug Macrolophus pygmaeus and the greenhouse...
Article
Full-text available
The epidemiological dynamics followed by viruses in protected horticultural crops in the Mediterranean Arc of Spain has evolved from a majority of those transmitted by aphids to the predominance of whitefly-transmitted ones. Later, due to the shift towards an integrated control that has been quite successful in the control of aleyrodids, not so muc...
Chapter
Several cucurbits species are cultivated in greenhouses worldwide. The most important genera are (1) Cucurbita, which includes squash, pumpkin, zucchini and gourds, (2) Citrullus, which includes watermelon and (3) Cucumis, which includes cucumber and various melons. Pests and diseases affecting cucurbit crops can vary considerably in relation to ge...
Article
During the last two decades, new criniviruses emerged in green bean crops in the south-east of Spain. Bean yellow disorder virus (BnYDV) was first detected in 2003 and caused major economic damage in crops grown in greenhouses. It was characterized as the first crinivirus to infect a member species of the Leguminosae family. Symptoms induced during...
Article
Tomato leaf curl New Delhi begomovirus (ToLCNDV) (genus Begomovirus, family Begomoviridae) and Cucumber green mottle mosaic tobamovirus (CGMMV) (genus Tobamovirus, family Virgaviridae) cause diseases in cucurbit crops and are of increasing importance in many parts of the world. Both virus species belong to different families and have different mode...
Article
Full-text available
(1) Background: Tomato leaf curl New Delhi virus (ToLCNDV), transmitted by tobacco whitefly (Bemisia tabaci Gennadius) (Hemiptera: Aleyrodidae), is of major concern in the cultivation of zucchini. The threat of this virus motivates reliance on chemical vector control but European consumers’ demands for vegetables grown free of pesticides provides a...
Article
Yellow sticky traps (YSTs) are commonly used in greenhouse crops to monitor flying pest species. Whiteflies like Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae) and Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) are typically monitored using YSTs in tomato and sweet pepper crops. By counting the whiteflies on a YST, growers get a...
Article
Tomato leaf curl New Delhi virus (ToLCNDV) is a whitefly-transmited virus that causes serious damage to different cultivated plant species mainly belonging to the Solanaceae and Cucurbitaceae families. The complex epidemiological factors associated with this disease make the seeking and use of genetically resistant varieties as one of the most effi...
Article
Full-text available
Cucumber green mottle mosaic virus (CGMMV) is a severe threat for cucumber production worldwide. At present, there are no cultivars available in the market which show an effective resistance or tolerance to CGMMV infection, only wild Cucumis species were reported as resistant. Germplasm accessions of Cucumis sativus, as well as C. anguria and C. me...
Article
Full-text available
Siguiendo el método Delphi se ha constituido y consultado a un panel de expertos sobre el estado de higiene de los cultivos hortícolas en invernadero, con especial interés en los problemas derivados de inputs y el manejo propio de los cultivos. A partir del cuestionario diseñado, hemos buscado la opinión sobre los problemas experimentados en los cu...
Article
Full-text available
Abstract Lettuce chlorosis virus-SP (LCV-SP) (family Closteroviridae, genus Crinivirus), is a new strain of LCV which is able to infect green bean plants but not lettuce. In the present study, high-throughput and Sanger sequencing of RNA was used to obtain the LCV-SP full-length sequence. The LCV-SP genome comprises 8825 nt and 8672 nt long RNA1 an...
Data
Primers list used to confirm the LCV-SP genome by RT-PCR. (DOC)
Data
Percentages of nucleotide sequence identity between the recombinants LCV-SP RNA1 (Event 1) and LCV RNA2 (Event 4) and the parentals. LCV-RNA1: FJ380118; BnYDV-RNA1: EU191904; LCV-SP-RNA2: MG489895; CCYV RNA2 904629. Parentals in the putative recombinants 2 and 3 are unknown. NA: not available. (DOC)
Data
RT-PCR identification of LCV-SP from virion purification. Lane 1: LCVSP-1F/LCVSP-2R; Lane 2: LCVSP-3F/LCVSP-4; Lane 3 LCVSP-54F/LC-Bn51R. (TIF)
Data
Numbers of reads aligning with a reference accession by BLASTN from contigs obtained from small RNAs and dsRNAs libraries. (DOC)
Data
Bootscan analysis with Simplot program using LCV-SP RNA1 as the query sequence. (TIF)
Data
Bootscan analysis with Simplot program using CCYV RNA2 as the query sequence. (TIF)
Preprint
Full-text available
Lettuce chlorosis virus-SP (LCV-SP) (family Closteroviridae, genus Crinivirus), is a new strain of LCV which is able to infect green bean plants and incapable of infecting lettuce crops. In the present study, high throughput and Sanger sequencing of RNA was used to obtain the LCV-SP full-length sequence. The LCV-SP genome comprises 8825 nt and 8672...
Article
Full-text available
Tomato leaf curl New Delhi virus (ToLCNDV) (family Geminiviridae, genus Begomovirus) has recently been introduced in western Mediterranean countries. Isolates in Spain constitute a new strain, denominated, ToLCNDV-ES, that is causing losses in commercial zucchini and melon crops, but it is also, although less, detected in commercial tomato crops. W...
Article
Full-text available
Melon necrotic spot virus (MNSV) es conocido como el virus de las manchas necróticas del melón o virus del “cribado” del melón, en referencia a las distintas manifestaciones durante la infección. Los síntomas en melón y pepino se caracterizan por la aparición de pequeñas manchas cloróticas de 0,5 a 2 mm en las hojas que evolucionan a necróticas. Ot...
Article
Full-text available
The complete RNA genome from Cucumber green mottle mosaic virus (CGMMV) (Alm08), collected during 2009 in cucumber crops located in Spain, was found to be 6,422 nucleotides long. The nucleotide sequence shared the highest identity with isolates from Russia (GQ495274, GQ495275, FJ848666) as do nucleotide sequences of partial CP and MP genes describe...
Article
Full-text available
Janssen D., Simon A., Crespo O., Ruiz L. (2017): Genetic population structure of Bemisia tabaci in Spain associated with Tomato leaf curl New Delhi virus – short communication. Plant Protect. Sci., 53: 25–31. Tomato leaf curl New Delhi virus (ToLCNDV) originates from Asia where it is persistently transmitted by indigenous cryptic species of the whi...
Article
Full-text available
Andalusia, southern Spain, is a major horticultural production region within the Mediterranean, where over 10,000 ha are dedicated to the production of pepper ( Capsicum annuum L.). Approximately two-thirds of the area dedicated to this crop is in a greenhouse and the remaining one-third is comprised of open field crops. Using pepper as a model, we...
Article
Full-text available
Tomato leaf curl New Delhi virus (ToLCNDV) (family Geminiviridae, genus Begomovirus) is an emerging virus in horticulture crops in Asia, and has recently been introduced in Spain, Tunisia and Italy. No betasatellite DNA was detected in infected tomato and zucchini squash samples from Spain and agroinoculated viral DNA-A and DNA-B were sufficient to...
Article
Full-text available
During the summer of 2015, leaf deformations and chlorotic mosaic symptoms on leaves and unusual necrotic lesions on leaves, stems and fruits were observed on pepper (Capsicum annuum) plants grown in Almeria (Spain). Symptomatic leaf and fruit samples from eight collected plants, carrying L3 and L4 resistance against tobamovirus, were analyzed by E...
Article
In May 2014, necrotic symptoms on leaves, stems, and fruits were observed in watermelon [Citrullus lanatus (Thomb.) Mansf. ‘Dulce maravilla’] produced in a commercial greenhouse from the province of Almeria, in Spain. Almost all plants from the greenhouse were severely affected, and over 80% died. Crude sap from symptomatic leaves was used for mech...
Article
Full-text available
The complete genome sequence of one isolate of Grapevine leafroll-associated virus 4 strain 9 (GLRaV-4 strain 9) was determined using a library constructed from the se-quencing of the small RNA population. The RNA genome consists of 13,858 nucleotides and contains seven open reading frames. A 5' non coding end (218 nt) is followed by ORF1 encoding...
Article
In September 2013, symptoms of chlorotic mottling and vein distortion on middle and lower leaves were observed in tomato (Solanum lycopersicum L.) growing in a greenhouse from the province of Almeria, Spain. A neighboring greenhouse had zucchini squash plants (Cucurbita pepo L.) with apical leaf curling symptoms and chlorotic mottling on intermedia...
Article
The introduction of invasive species causes damages from the economic and ecological point of view. Interception of plant pests and eradication of the established populations are two management options to prevent or limit the risk posed by an invasive species. Management options generate costs related to the interception at the point of entry, and...
Article
In September 2011, symptoms typically associated with Bean yellow disorder virus (BYDV) such as intervenal mottling and yellowing on middle and lower leaves combined with brittleness were observed in green bean (Phaseolus vulgaris L.) produced in commercial greenhouses from Granada and Almeria provinces, Spain. The affected plants were all observed...
Article
Full-text available
Bean Yellow Disorder Virus (BnYDV) es un miembro del género Crinivirus (familia Closteroviridae) descrito recientemente como el primero de estos virus que infecta a miembros de la familia Fabaceae. Fue identificado por primera vez en el año 2003 en cultivos de judía de invernadero en la provincia de Almería. En este trabajo, se analizan las secuenc...
Article
Cucumber vein yellowing virus (CVYV) (genus Ipomovirus, family Potyviridae) and Cucurbit yellow stunting disorder virus (CYSDV) (genus Crinivirus, family Closteroviridae) are included in the EPPO A2 Action List of pathogens and have become a limiting factor of cucumber production in the southeast of Spain. Due to the importance of these two pathoge...
Article
Full-text available
The complete genome of a severe isolate of Cucumber vein yellowing virus (CVYV) from Jordan was sequenced. Comparison with the genome of a Spanish CVYV isolate inducing very mild symptoms in cucumber cultivars revealed a nucleotide identity of 94% for the complete genome and an amino acid identity of 96% for the coding region. Comparison of synonym...
Article
In Spain, the province of Almeria has 30,000 ha of greenhouses available for the production of vegetables. Currently, in over half of this area, plant health is managed using biological control. A small but growing proportion of out-of-season vegetables are grown following ecological farming protocols but there are doubts as to the economic feasibi...
Article
Full-text available
Orius laevigatus Fieber, se utiliza en los programas de manejo integrado en cultivo de pimiento para el control de trips (Frankliniella occidentales pergande). Su suelta se realiza cuando el cultivo está en floración y requiere un periodo entorno a cuatro semanas para establecerse sobre el cultivo. una solución para adelantar su instalación podría...
Article
Full-text available
In Almeria (Spain) the area of greenhouses dedicated to the production of French bean has reduced considerably during the recent years due to more competitive production in neighbouring countries, and after the introduction in 2003 of Bean yellow disorder virus (BnYDV) transmitted by the sweet potato whitefly Bemisia tabaci. Based on the European r...
Article
Full-text available
Zucchini squash is host to Cucurbit yellow stunting disorder virus (CYSDV), a member of the genus Crinivirus, and Cucumber vein yellowing virus (CVYV), a member of the genus Ipomovirus, both transmitted by the whitefly Bemisia tabaci. Field observations suggest the appearance of new symptoms observed on leaves of zucchini squash crops when both vir...
Article
Abstract Bean yellow disorder virus (BnYDV) was recently identified as the first crinivirus (family Closteroviridae) that infects members of the family Leguminosae. It was first observed during the autumn of 2003, causing heavy losses in French bean (Phaseolus vulgaris L.) grown commercially in Spain. The virus is transmitted by the sweetpotato whi...
Article
Full-text available
RESUMEN Un ensayo basado en tecnología Taqman® ha sido desarrollado para el estudio epi-demiológico de los dos virus de mayor incidencia e impacto económico presentes en los cultivos de cucurbitáceas en la región de Almería. A través del análisis de forma indivi-dual de su vector, la mosca blanca Bemisia tabaci, ambos virus fueron detectados y cuan...
Article
Full-text available
Cucumber vein yellowing virus (CVYV) has become a limiting factor for cucumber production in greenhouses in southeast Spain. Current protection measures rely on control of the vector, the whitefly Bemisia tabaci, and the use of resistant cultivars. Reverse transcription followed by real-time PCR assays based on TaqMan® chemistry have been developed...
Article
Full-text available
Abstract: Preliminary results have identified Mentha suaveolens and Dittriehia viscosa as promising candidates for use as refuges for enhancing the activity of predators on protected crops. The first one as a refuge for the flower bug Orius laevigatus and the second as a refuge for the mirid bug Nesidioeoris tenuis, in the southeast of Spain. Augme...
Article
Abstract: Preliminary results have identified Mentha suaveolens and Dittriehia viscosa as promising candidates for use as refuges for enhancing the activity of predators on protected crops. The first one as a refuge for the flower bug Orius laevigatus and the second as a refuge for the mirid bug Nesidioeoris tenuis, in the southeast of Spain. Augme...
Article
Full-text available
Abstract: Preliminary results have identified Mentha suaveolens and Dittriehia viscosa as promising candidates for use as refuges for enhancing the activity of predators on protected crops. The first one as a refuge for the flower bug Orius laevigatus and the second as a refuge for the mirid bug Nesidioeoris tenuis, in the southeast of Spain. Augme...
Article
Full-text available
A study was conducted to determine the identity and prevalence of viruses in 455 greenhouses in the main Spanish green bean growing area. Directed surveys were conducted in 422 crops from 2000–2004 to collect samples from diseased plants displaying symptoms that could be attributed to viruses. The samples were analysed to detect any virus by means...