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38
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Introduction
Dipayan Bose currently works at University of Pennsylvania. Dipayan does research in EBV and KSHV infections and the different events occuring subsequently and also working on Microbiome project My most recent publication is 'Heat Killed Attenuated Leishmania Induces Apoptosis of HepG2 Cells Through ROS Mediated p53 Dependent Mitochondrial Pathway'.
Current institution
Additional affiliations
September 2018 - present
April 2018 - September 2018
Publications
Publications (38)
Epstein-Barr virus (EBV) employs various strategies for long-term survival, including the expression of non-coding RNAs (ncRNAs). This study uncovers and characterizes two novel EBV-encoded ncRNAs, p7 and p8, which are upregulated during lytic reactivation and interact with both viral and host genomes. These ncRNAs bind to cellular RNA transcripts,...
Oncogenic gamma herpesviruses, including Epstein–Barr Virus (EBV) and Kaposi’s Sarcoma-associated Herpesvirus (KSHV), are opportunistic cancer-causing viruses and induces oncogenesis through complex mechanisms, which involves manipulation of cellular physiology as well as epigenetic and epitranscriptomic reprogramming. In this review, we describe t...
Oncoviruses play a role in approximately 15% or more of all human cancers. A significant portion of the global population carries at least one of these oncoviruses, yet only a small percentage of these individuals progress to the development of cancer. The intricate interplay between host and viral factors constitutes a complex process that collabo...
Most mature B-cell malignancies originate from the malignant transformation of germinal center (GC) B cells. The GC reaction appears to have a role in malignant transformation, in which a major player of the GC reaction is BCL6, a key regulator of this process. We now demonstrate that BCL6 protein levels were dramatically decreased in Epstein-Barr...
Hypoxia results from an insufficient supply of oxygen, which results in physiological stress in biological systems. Cells respond to this unfavorable condition by reducing critical cellular functions, which include replication, transcription, and translation. Oncogenic Kaposi sarcoma-associated virus (KSHV) undergoes lytic reactivation in hypoxic c...
Background
Reactivation of Epstein Barr virus (EBV) leads to modulation of the viral and cellular epitranscriptome. N6-methyladenosine (m ⁶ A) modification is a type of RNA modification that regulates metabolism of mRNAs. Previous reports demonstrated that m ⁶ A modification affects the stability and metabolism of EBV encoded mRNAs. However, the ef...
The biphasic life cycle (latent and lytic) of Kaposi’s sarcoma-associated Herpesvirus (KSHV) is regulated by epigenetic modification of its genome and its associated histone proteins. The temporal events driving epigenetic reprogramming of the KSHV genome on initial infection to establish latency has been well studied, but the reversal of these epi...
Recently unfolded mechanisms showed lipid droplet helps in pathogen survival and paralyzes host immune response. In the present study, we showed the extent of lipid droplet(LD) generation in Leishmania donovani infection, the signaling involved, and their function concerning pathogenicity. RAW 264.7 and J774A.1 cells were used to infect with L. don...
Alterations to the natural microbiome are linked to different diseases, and the presence or absence of specific microbes is directly related to disease outcomes. We performed a comprehensive analysis with unique cohorts of the four subtypes of breast cancer (BC) characterized by their microbial signatures, using a pan-pathogen microarray strategy....
The cellular adaptive response to hypoxia, mediated by high HIF1α levels includes metabolic reprogramming, restricted DNA replication and cell division. In contrast to healthy cells, the genome of cancer cells, and Kaposi's sarcoma associated herpesvirus (KSHV) infected cells maintains replication in hypoxia. We show that KSHV infection, despite pr...
The hypoxic microenvironment and metabolic reprogramming are two major contributors to the phenotype of oncogenic virus infected cells. Infection by Kaposi’s sarcoma associated herpes virus (KSHV) stabilizes hypoxia inducible factor 1α (HIF1α) and reprograms cellular metabolism. We investigated the comparative transcriptional regulation of all majo...
The novel coronavirus outbreak started in December 2019 and rapidly spread around the globe, leading to a global pandemic. Here we reported the association of microbial agents identified in oropharyngeal and nasopharyngeal samples from patients with SARS-CoV-2 infection, using a Pan-microarray based technology referred to as PathoChIP. To validate...
Dysbiotic microbiomes are linked to many pathological outcomes including different metabolic disorders like diabetes, atherosclerosis and even cancer. Breast cancer is the second leading cause of cancer associated death in women, and triple negative breast cancer (TNBC) is the most aggressive type with major challenges for intervention. Previous re...
Hepatocellular carcinoma (HCC), is the most leading cause of cancer‐related death in all over the world (seems to more than 80% of death cases). It is frequently linked with continuous hepatocytes death, inflammatory cell infiltration, and compensatory liver regeneration with higher angiogenic rate. Considering the different signalling process duri...
Lipid droplets (LD) are newly characterized dynamic cytoplasmic organelle which is the storehouse of different immunosuppressive cytokines and enzymes like cyclooxygenase and lipoxygenase. Tumors are known to modulate the immune system by immune-editing the microenvironment. Immuno-editing comprises of three steps namely cancer immune-surveillance,...
Background/aims:
Cytotoxic effect of attenuated Leishmania on liver cancer cells by inducing ROS generation.
Methods:
Spectrophotometric study to analyze cell death and levels of different active caspases. Flow cytometric study was done to analyze apoptosis induction and ROS generation and levels of different protein. Western blot analysis was p...
Promastigote form of Leishmania, an intracellular pathogen, delays phagosome maturation and resides inside macrophages. But till date limited study has been done to manipulate the phagosomal machinery of macrophages to restrict Leishmania growth. Attenuated Leishmania strain exposed RAW 264.7 cells showed a respiratory burst and enhanced production...
Very often conventional therapy, i.e. chemotherapeutic treatment, develops resistance in cancer cells and fails to be effective against disease states. An alternative strategy or a new entity may resolve the problem. Interestingly, the microbial world has begun to be explored in medicinal research as a potential new source to deliver bio-active mol...
We have synthesized and structurally characterized a new doubly chloro bridged dimeric copper(II) complex, [Cu2(µ-Cl)2(HL)2Cl2] (1) based on a Schiff base ligand, 5-[(Pyridin-2-ylmethylene)-amino]-pentan-1-ol). Single crystal X-ray diffraction study shows presence of dinuclear copper(II) centres in square pyramidal geometry linked by obtuse double...
The microbial source, which includes live, attenuated, or genetically modified microbes or their cellular component(s) or metabolites, has gained increasing significance for therapeutic intervention against several pathophysiological conditions of disease including leukemia, which remains an incurable disease till now despite recent advances in the...
Sphingolipids are membrane and intracellular lipids that typically modulate cellular processes to cause cell death. Exogenous administration of sphingolipids may cause restriction of tumour growth and several alternative strategies are being used to control the cell growth. The microbes, their cellular component(s) or metabolites like DHA, EPA and...
Persistence of liver injury alters the internal milieu, promotes deregulation of inflammatory factors, and leads to dysplastic lesions like fibrosis, cirrhosis to hepatocellular carcinoma. Our previous study revealed that leishmanial lipid (pLLD) exerts potential anti-inflammatory activity in sepsis associated hepatic injury. We now show that pLLD...
Sepsis is the reflection of systemic immune response that manifests in the sequential inflammatory process in presence of infection. This may occur as a result of gram-negative bacterial sepsis including Escherichia coli infection that gives rise to excessive production of inflammatory mediators and causes severe tissue injuries. We have reported e...
The use of live, attenuated, or genetically modified microbes or their cellular component(s) or metabolites has begun to emerge as a potential new approach in medicinal research to deliver biologically active entities. Thus, advancing our knowledge of such microbe-mediated therapy may suggest new avenues for therapeutic intervention in many disease...
Anticancer role of andrographolide is well documented. To find novel potent derivatives with improved cytotoxicity than andrographolide on cancer cells, two series of di-spiropyrrolidino- and di-spiropyrrolizidino oxindole andrographolide derivatives prepared by cyclo-addition of azomethine ylide along with sarcosine or proline (viz. sarcosine and...
Analysis of caspase-3 and caspase-9, DNA fragmentation, mitochondrial membrane potential and cytochrome c level Cells (2×105) after treated with 20 µM of CY2 for 24 h by ELISA based colorimetric assay using kits in HepG2 and MiaPaCa2. Enhancement of O.D. represents activation of (A) caspase-3, (B) caspase-9. (C) DNA fragmentation (D) Mitochondrial...
Analysis of apoptosis by flow cytometry in HepG2 and MiaPaCa-2 annexin-V/FITC. Cells were treated with 20 µM of CY2 (left panel) and andrographolide (right panel) for 24 h. Binding of annexin V to phosphatidyl serine was determined by flow cytometry. Percentages of apoptotic cells determined by the number of annexin V (+)/propidium iodide cells are...
Study of cell cycle arrest by propidium iodide in HepG2 and MiaPaCa-2. Cells treated with or without 20 µM of CY2 and andrographolide for 24 h were used for cell cycle arrest study as described in materials and methods. Percentage of G0–G1 cell population increases after treatment of indicating G1/S phase cell cycle arrest. (A) Cell cycle arrest in...
A relative ROS generation by 20 µM of CY2 and andrographolide with the scavenging action by NAC in HepG2 and MiaPaCa-2 cell lines. Flourosence intensity of 2′,7′-dichlorofluorescein (DCF) in HepG2 and MiaPaCa-2 cell lines after treatment of 20 µM of CY2 and andrographolide incubated for 24 h in treated without NAC and NAC (Values are mean ± S.D. an...
Viability of three cancer lines HCT116, MiaPaCa-2, HepG2 and a non–cancer cell line Chang liver cells was observed in response to andrographolide and its derivatives. Experimental cells (2×105) were treated with andrographolide and its derivatives. Assay of dead cells by trypan blue exclusion test. Cells, untreated or treated with different concent...
Viability of PBMC in response to CY2, CY14 and CY15 for 36 h. The GI50 values where calculated from MTT assay Values are mean ± S.D. and represent one of the 3 representative experiments (P<0.001).
(TIF)
Different quinazoline derivatives have showed wide spectrum of pharmacological activities. Some 3-(arylideneamino)-phenylquinazoline-4(3H)-ones have been reported to possess antimicrobial activity. The present study has been undertaken to evaluate the anticancer effect of these quinazolinone derivatives. The quinazolinone derivatives were synthesiz...
Novel Au(I)-N-heterocyclic carbene complexes, 1-methyl-3-(2-pyridylmethyl)-benzimidazolylidenegold(I)-chloride, 1; 1-benzyl-3-(2-pyridylmethyl)-benzimidazolylidenegold(I)chloride, 2; and Pt(II)-N-heterocyclic carbene complexes 1-methyl-3-(2-pyridylmethyl) benzimidazolylidene platinum(II)chloride, 3; and 1-benzyl-3-(2-pyridylmethyl) benzimidazolylid...
Four water-soluble dinuclear Zn(II) complexes (1-4) of compartmental ligand L = 2,6-bis(R-iminomethyl)-4-R'-phenolate (where R = N-ethylpiperidine or R = N-ethylpyrrolidine, R' = methyl or tert-butyl) have been synthesized, characterized, and their DNA cleavage activity and cytotoxicity toward HepG2 cancerous cells are evaluated. The dinuclear comp...
Questions
Question (1)
I am studying change in some specific gene expression in particular cancer samples and I am comparing that with the expression of the same gene in non cancerous part of the same patient. But I also want to analyse the expression of those genes in normal individuals. But for that I am not getting any reference sample based on which I can get the fold change increase for he normal individuals. please suggest me a protocol by which I can represent the gene expression of the normal individuals.
Thanks..
