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Diogenes Infante

Diogenes Infante
BioFarm Urbarn Farm · Leafy Vegetables Production

PhD
Managing Director, Biofarm Urban Agriculture

About

47
Publications
22,029
Reads
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1,365
Citations
Introduction
BioFarm, an intelligent urban farm in Caracas, Venezuela. First Urban Farm in Venezuela. Production of lettuces and microgreens in a controlled urban environment.
Additional affiliations
June 2014 - present
Programa Prometeo, Senescyt, Ecuador
Position
  • High Level 1 Investigator
June 2014 - December 2014
Fundación Instituto de Estudios Avanzados
Position
  • High Level 1 Investigator
January 2003 - present
Centro de Investigación Científica de Yucatán
Education
March 1989 - February 1990
Memorial Sloan Kettering Cancer Center
Field of study
  • Molecular Biology
March 1987 - February 1989
Roche Institute of Molecular Biology
Field of study
  • Plant Molecular Biology
September 1983 - February 1987
Université Paris-Sud 11
Field of study
  • Bacterial Molecular Biology

Publications

Publications (47)
Article
Full-text available
The response of a genotype each of C. moll e and C. macrocarpum to drought (low soil moisture availability) was studied in a seasonally dry tropical environment throughout 3 consecutive years. Changes in soil water content, leaf water relations and gas exchange, chlorophyll fluorescence, forage yield and leaf area index (LAI) were compared in well-...
Article
Full-text available
p> Título en ingles: Biochemical characterization of leaves in Theobroma cacao clones and its relation with trichomes Theobroma cacao L. es un cultivo de gran importancia por sus posibles aplicaciones en la farmacia y la agricultura. Contiene metabolitos que resultan de gran interés por sus funciones biológicas naturales en la respuesta de la pla...
Article
Full-text available
Evaluation of genetic diversity in nine species of Cattleya using RAPD and ISTR molecular markers Genetic diversity of nine species of Cattleya spp. collected in Venezuela was studied using RAPD and ISTR molecular markers. Out of 49 RAPD primers initially used to identify polymorphic markers, only 19 showed higher resolution and number of discrimin...
Article
Full-text available
Genetic diversity of nine species of Cattleya spp. collected in Venezuela was studied using RAPD and ISTR molecular markers. Out of 49 RAPD primers initially used to identify polymorphic markers, only 19 showed higher resolution and number of discriminative polymorphic bands in agarose gels. Two-hundred fifty-five-bands were generated, from which 1...
Article
Full-text available
This protocol permits the simultaneous extraction of clean DNA from many samples with little reagent waste, thus decreasing the cost of analysis per sample. The procedure is rapid, permitting the processing of 80–100 samples per day. Using this protocol, we analyzed naturally propagated and micropropagated populations of henequen and otherAgavaceae...
Article
Full-text available
Background: Cassava (Manihot esculenta Crantz) is a crop that is high in carbohydrates in the roots and in protein in the leaves, important for both human consumption and animal feed, and also has a significant industrial use for its starches. In this study we evaluated the genetic variability with molecular markers in different stages in microprop...
Article
Full-text available
Two DNA-based marker systems, amplified fragment legnth polymorphism (AFLP) and inverse sequence-tagged repeat (ISTR), were evaluated with respect to their discriminating capacity and efficiency in genetically analyzing henequen (Agave fourcroydes). Samples were taken from a mother plant, sucker-derived daughter plants, and bulbils. ISTR analysis p...
Article
Full-text available
A detailed and curated map of molecular interactions taking place in the polyphenols (flavonoids) biosynthesis in Theobroma cacao L. is presented. The map was created using the software Cytoscape v.2.7 and the KEGG (Kyoto Encyclopedia of Genes and Genomes) database. The statistical parameters of the network were determined with CentiScape v.1.1 and...
Article
Full-text available
We sequenced and assembled the draft genome of Theobroma cacao, an economically important tropical-fruit tree crop that is the source of chocolate. This assembly corresponds to 76% of the estimated genome size and contains almost all previously described genes, with 82% of these genes anchored on the 10 T. cacao chromosomes. Analysis of this sequen...
Article
Full-text available
We sequenced and assembled the genome of Theobroma cacao, an economically important tropical fruit tree crop that is the source of chocolate. The assembly corresponds to 76% of the estimated genome size and contains almost all previously described genes, with 82% of them anchored on the 10 T. cacao chromosomes. Analysis of this sequence information...
Article
Full-text available
RESUMEN Dado que la eficacia de los distintos protocolos existentes para aislar ADN en cantidad y calidad suficiente puede diferir según el cultivo, es necesario estandarizar los métodos de extracción para garantizar su sencillez y efectividad. En este trabajo se propone una modificación al método de Risterucci et al. (2000) el cual fue comparado c...
Article
Full-text available
Since the effectiveness of the different protocols for isolating DNA in sufficient quantity and quality may vary depending on the crop, it is necessary to standardize the extraction methods to ensure its simplicity and effectiveness. In this paper we propose a modification to the method of Risterucci et al. (2000), which was compared with other pro...
Article
Full-text available
Agave tequilana is a species of great economic importance for the Mexican society. Like most agaves, it can be propagated either sexually or asexually. However, plants originating from rhizomes are mainly used for commercial production. The aim of this study was to determine the genetic fidelity of plants that were obtained from the field and propa...
Article
Full-text available
A study was conducted on the effect of different concentrations of sodium chloride (NaCl) on seedlings of Stylosanthes guianensis cv. CIAT-184 and Centrosema molle, cultivated on topsoil. Fifteen days after the treatment fresh mass, dry mass, root/shoot ratio and biomass distribution were determined, as well as the concentration of soluble phenols,...
Article
Full-text available
Drought is one of the factors having a negative effect on the cultivation of legumes. The objective of this work was to study the response of P. vulgarís, in Cuba and Venezuela, to water stress. Seeds were previously disinfected and grouped into three treatments: 70 %, 40 % and 20 % field capacity (FC). Results carrespond to three replicates per tr...
Article
Full-text available
A study was conducted on the effect of different concentrations of sodium chloride (NaCl) on seedlings of Stylosanthes guianensis cv. CIAT-184 and Centrosema molle, cultivated on topsoil. Fifteen days after the treatment fresh mass, dry mass, root/shoot ratio and biomass distribution were determined, as well as the concentration of soluble phenols,...
Article
Full-text available
The systematic study of the genetic fingerprint (genomics) and the biochemistry (metabolites) that goes with a specific cellular process requires the characterization of all the small molecules that form the profile of metabolites and the associated genes. The metabolome represents the collection of all the metabolites during certain process in an...
Article
The systematic study of the genetic fingerprint (genomics) and the biochemistry (metabolites) that goes with a specific cellular process requires the characterization of all the small molecules that form the profile of metabolites and the associated genes. The metabolome represents the collection of all the metabolites during certain process in an...
Article
Full-text available
Centrosema molle Mart. ex Benth. (sin. C. pubescens Benth.) is among the most important tropical legumes used for forage and green cover. With the purpose of studying the genetic variability of a germplasm collection of the species, 34 accessions were evaluated using four AFLP primer combinations. Additionally, the association between the genetic g...
Conference Paper
Full-text available
Agaves are succulent monocot plants rich in fibers, sugars and other important compounds. They are also valued as ornamental plants and for their ability to grow in poor soils. Molecular markers (AFLP and ISTR) were used to study genetic diversity in different Agavaceae plant samples. The comparison of the banding pattern between the mother plant a...
Article
Full-text available
Agaves are succulent monocot plants rich in fibers, sugars and other important compounds. They are also valued as ornamental plants and for their ability to grow in poor soils. In the present study, inverse sequence-tagged repeats (ISTR) and amplified fragment length polymorphism (AFLP) analysis were used to study genetic diversity in differentAgav...
Article
Full-text available
Amplified fragment-length polymorphism (AFLP) was used to evaluate the stability of DNA in regenerated plantlets of Coffea arabica obtained by direct (DSE) and indirect somatic embryogenesis (ISE). Cluster analysis using the unweighted pair-group method (UPGMA), showed no specific grouping pattern related to the type of embryogenesis. These results...
Article
Full-text available
Henequen, Agave fourcroydes Lem, is a plant whose fiber is employed in the manufacture of agricultural twine, sacks, carpets and other products. It is a pentaploid (5×) with a long life cycle of up to 20 years that flower only once towards the end of its life and does not produce viable seeds. Since it is asexually propagated through the production...
Article
Full-text available
El trabajo tuvo como finalidad estudiar el crecimiento micelial y la obtención de fructificación de 3 especies de hongos comestibles orejas blancas, Pleurotus ostreatus (Jacq.:Fr.) Kumm., shiitake japonés, Lentinus edodes (Berk.) Sing y strofaria gigante, Stropharia rugosoannulata Farl.ex Murr, en los 20 diversos substratos provenientes de desechos...
Article
Full-text available
This protocol permits the simultaneous extraction of clean DNA from many samples with little reagent waste, thus decreasing the cost of analysis per sample. The procedure is rapid, permitting the processing of 80-100 samples per day. Using this protocol, we analyzed naturally propagated and micropropagated populations of henequen and other Agavacea...
Article
Full-text available
Henequen is a perennial monocot that produces flowers only once towards the end of its long life cycle and then dies. Its high levels of ploidy (5x) render it sterile. It produces seeds with very low viability in the laboratory. Therefore, in plantations it is multiplied only through vegetative propagation using rhizomes. In previous work using AFL...
Article
Wild tomato plants of Lycopersicon peruvianum var. dentatum were transformed with pG11 plasmid having human beta-interferon (hIFN-beta) and NPTII genes. Transformation was demonstrated by polymerase chain reaction (PCR) and ELISA assay. Expression of hIFN-beta gene was identified by Western blot analysis. Transgenic plants produced seeds and F1 gen...
Article
In this work we studied tomato yellow mosaic geminivirus (ToYMV) symptoms and DNA presence in several species of Solanaeae: Lycopersivon esculentum, Lycoperstcon peru-vianum. Lycium barbarum, Nicotiaiiu benthautian, Solunum lycopersioides. Solanum quitoense and Solanum rickli. After mechanical and insect transmission, in both greenhouse and open fi...
Article
Full-text available
Diseases caused by geminiviruses are widespread in the tropical-subtropical tomato productionareas. Tomato yellow mosaic geminivirus (ToY:MV) is a member of the whitefly-transmitted subgroup (III), which is vectored by the whitefly Bemisia tabaci. Several accessions of Lycopersicon chilense, L. hirsutum, and L. peruvianum var. glandulosum were iden...
Article
Full-text available
Previous studies from this laboratory (Schroy, P., Rifkin, J., Coffey, R.J., Winawer, S., and Friedman, E. (Cancer Res., 50: 261-265, 1990; Schroy, P.C., Winawer, S., and Friedman, E. Cancer Lett., 48: 53-58, 1989) found that a 7-day treatment of the human colon carcinoma cell line HT29 with the differentiation agent hexamethylene bisacetamide (HMB...
Article
In the diploid vegetative plant cell, the nuclear DNA is present in two copies, whereas the chloroplast and mitochondria genomes are present in a higher and variable copy number. We have studied the replication of the nuclear, chloroplast and mitochondrial DNA in cultured Nicotiana tabacum cells using density and radioactive markers. Essentially al...
Article
Full-text available
Colon tumor cells are more responsive to certain growth modulators in their local environment in vivo than are normal colonocytes. Examples of this class of compounds are the fecal diglycerides (DGs)(E. Friedman et al., Cancer Res., 49: 544-548, 1989), which may act as endogenous tumor promoters. At the concentration found in vivo, fecal DGs compos...
Chapter
Until recently the study of nitrogen fixation genes from Rhizobium has focused either on nif genes as they have been defined in Klebsiella pneumoniae (Dixon, 1984), or on fix genes closely linked to nif genes (such as fixABC, Pühler et al., 1984; Ausubel et al., 1985). However we may expect some non nif functions to be important for nitrogen fixati...
Chapter
Alfalfa is the cultivated temperate legume which fixes the largest amount of nitrogen and produces the largest amount of protein per hectare. Nitrogen fixation takes place in specialized organs, the root nodules, the formation of which involves the co-differentiation of both symbiotic partners. Dissection of this developmental process requires a jo...
Article
En el momento de la consolidación definitiva de la biotecnología agrícola (1995), con la introducción comercial de sus primeros productos en los países líderes, es apropiado un análisis de su estado de arte en América Latina y el Caribe (ALC). Los objetivos del presente trabajo (ENV-105) son la determinación de sus avances, la caracterización de su...

Network

Cited By
    • Escuela Superior Politécnica del Litoral (ESPOL)
    • French National Institute for Agriculture, Food, and Environment (INRAE)
    • Agriculture & Food Systems Institute
    • French National Institute for Agriculture, Food, and Environment (INRAE)
    • Cirad - La recherche agronomique pour le développement

Projects