Dina Orozco-Davila

Autonomous University of Chiapas · Instituto de Biociencias

The Genetic Sexing Strain (GSS) of Anastrepha ludens (Loew) Tapachula-7 (Tap-7) produces males emerged from brown pupa [used for the Sterile Insect Technique (SIT)] and females from black pupa (a by-product) that can be sorted by a mechanical process. The early sorting provides young black pupae which were assessed as host of the parasitoid Coptera...

The sterile insect technique (SIT) is an effective, environmentally friendly method for insect control whose success depends on the sexual performance and survival of sterile males. These two parameters are influenced by environmental conditions of target areas, and releasing insects with a higher tolerance to stressful environments can improve SIT...

The sterile insect technique (SIT) requires the rearing, sterilization, and release of large numbers of insects to induce sterility within wild pest populations. In 1992, the Mexican government launched a national campaign against economically important fruit fly pests, with the aim of establishing free areas and strengthening fruit exports. To ach...

Mating at dusk, Anastrepha ludens (Diptera: Tephritidae)

• Tapachula‐7 is a genetic sexing strain of Anastrepha ludens (Loew) (Diptera: Tephritidae) developed for the application of the sterile insect technique (SIT). To improve the sexual performance of this strain, a mass‐reared colony was established from males selected for their sexual competitiveness. • Males from selected colonies are more sexually...

La levadura inactiva en polvo es el único ingrediente disponible como fuente de proteína, en las dietas larvarias para la cría masiva de todas las especies de moscas de la fruta, proporcionando una proteína de alto valor biológico, vitaminas del complejo B, carbohidratos y grasas esenciales. Además, es el ingrediente con mayor costo, por lo cual se...

The inactive yeast powder is the single ingredient available as sources of protein in the larval diets for mass rearing of all species of fruit flies, providing a protein of high biological value, B vitamins, carbohydrates and essential fats. In addition, it is the most expensive ingredient, thus we are continuously looking for low-cost alternative...

One major constraint in the development and implementation of a successful and cost-effective area-wide integrated pest management (AW-IPM) programme with a SIT component for Bactrocera oleae (Diptera: Tephritidae) is the ability to produce a large number of high quality mass-reared individuals. The aim of this study was to develop a more efficient...

The sexual performance of Anastrepha ludens males of the Tapachula-7 genetic sexing strain, produced via selection based on mating success, was compared with that of males produced without selection in competition with wild males. Mating competition, development time, survival, mass-rearing quality parameters and pheromone production were compared....

Tephritid pests controlled through the sterile insect technique (SIT) are mass-reared and subsequently released in affected areas. Several quality parameters are currently used to test adults, but none take into account interactions with a predator. When sterile males are released in the field, they will need to avoid predators until they reach sex...

A genetic sexing strain of Anastrepha ludens (Loew), Tapachula-7, was developed by the Mexican Program Against Fruit Flies to produce and release only males in programs where the sterile insect technique (SIT) is applied. Currently, breeding are found at a massive scale, and it is necessary to determine the optimum irradiation dose that releases st...

A new adult diet formulation was evaluated for sterile Anastrepha ludens (Loew) and Anastrepha obliqua (Macquart) males at the emergence and release facility of fruit flies in Mexico. The formulation consists of hydrolyzed protein, sugar, juvenile hormone analogue methoprene, and water. The proportion of the ingredients between the solute (4% hydro...

In this study, the effects of relaxed mass-rearing conditions on Anastrepha obliqua (Macquart) production and quality were determined. Relaxed rearing conditions were defined by a reduction in the density of adult flies, from 80,000 to 60,000 flies per mass-rearing cage, and a reduction in the density of larvae, from 6.18 to 3.70 eggs/g of diet. In...

In this study, rearing systems for, and the quality and demographic parameters of, a wild strain (WS) and two laboratory strains (LSs; one maintained on a torula yeast-casein diet and the other on a starter-gel diet) were determined for the American guava fruit fly Anastrepha striata (Schiner). No differences were observed between the LSs, but ther...

The sterile insect technique (SIT) is currently used to control Mexican fruit fly Anastrepha ludens Loew (Diptera: Tephritidae). However, mass-rearing can alter the quality of released males. If males that are mass-reared have behaviours different from those of their wild counterparts, then this may diminish the effectiveness of SIT. Questions rema...

The “Mexican fruit fly” Anastrepha ludens (Loew), is an economically important pest of citrus and mango crops in Mexico. Sexual behavior studies on mutant strains can help in the selection of insects with potential for release in field. In this study we evaluated the visual response of the mutant “yellow eyes” of A. ludens by color choice experimen...

A new genetic sexing strain of the Mexican fruit fly, Anastrepha ludens (Loew), was evaluated in tests of sexual behavior to determine its possible application using the sterile insect technique. Tests in field cages measuring time to sexual maturity, compatibility with wild flies, and competitiveness were compared between the genetic sexing strain...

Standard quality control tests were carried out to evaluate the sexual performance of laboratory sterile Anastrepha obliqua males acclimated under field conditions. Laboratory sterile males were maintained in a mango orchard in wooden cages for 0, 2, 4, 6 and 8 days before competing with wild males for matings with wild females in sexual performanc...

An artificial larval diet for Anastrepha striata (Schiner) was developed and the changes in the rearing and quality parameters through 6 generations during the adaptation were characterized. In the first experiment we tested diet formulations that had already been developed for the mass-rearing of Anastrepha ludens (Loew), A. obliqua (Macquart),A....

Recent fears of terrorism have provoked an increase in delays and denials of transboundary shipments of radioisotopes. This represents a serious constraint to sterile insect technique (SIT) programs around the world as they rely on the use of ionizing radiation from radioisotopes for insect sterilization. To validate a novel X ray irradiator, a ser...

La dispersión y longevidad de las moscas estériles silvestres y de cría masiva de Anastrepha ludens (Loew) y A. obliqua (Macquart) fueron determinadas y comparadas utilizando un arreglo rectangular de 64 trampas espaciadas a 60 metros entre trampas alrededor del punto de liberación en Tapachula Chiapas, México. Las trampas fueron revisadas y evalua...

We compared the biology and sexual behavior between a strain of laboratory with wild phenotype (LFS) and a strain of laboratory with mutant phenotype (LFM) of Anastrepha ludens (Loew), characterized by clear coloration of the body and creamy yellow eyes to determine their potential as genetic marker in the control programmes through the use of the...

Se comparó la biología y el comportamiento sexual entre una cepa de laboratorio con fenotipo silvestre (LFS) con una cepa de laboratorio con fenotipo mutante (LFM) de Anastrepha ludens (Loew), que se caracteriza por una coloración del cuerpo clara y ojos amarillo cremoso, para determinar su potencial como marcador genético en los programas de contr...

The mass-reared colony of Anastrepha ludens (Loew) currently used in Mexico for suppression of the Mexican fruit fly has been in use for over 10 years. Sterile flies are released into a wide range of environmental conditions as part of an integrated area-wide approach to suppress diverse populations of this pest in the Mexican Republic. This paper...

The effect of time of pupariation on pupal weight and adult sexual competitiveness under field cage conditions was studied in mass-reared Anastrepha ludens (Loew) males. Larvae that took 72 h to pupariate after separation from diet resulted in lighter pupae than those that took 24 and 48 h. Wild pupae were heavier than the 48- and the 72-h pupae bu...

Several studies have suggested that maintaining a line of insects under laboratory conditions reduces their biological attributes. With this principle in mind, the mass production of Anastrepha ludens originating from a colony raised under relaxed rearing conditions was evaluated over a period of three years. The results of the evaluation indicated...