Dimitris Kouretas

University of Thessaly | UTH · Τμήμα Βιοχημείας και Βιοτεχνολογίας

Spirulina plantensis is a popular supplement which has been shown to have antioxidant and performance enhancing properties. The purpose of this study was to evaluate the effects of spirulina supplementation on (a) redox status (b) muscle performance and (c) muscle damage following an eccentric bout of exercise that would induce muscle damage. Twent...

A feeding trial involving growing piglets was undertaken to establish whether feed supplemented with whey protein concentrate (WPC), exhibiting antioxidant properties, had any effects on welfare and meat quality. For that purpose, 48 weaned piglets (20-days-old) were assigned to two experimental groups receiving standard or experimental diet for 30...

In the present study, grape pomace (GP) was used as feed additive in the diet of weaned piglets in order to develop innovative feedstuffs and to investigate their potential beneficial effects on welfare, productivity and meat quality. For examining the antioxidant capacity of the experimental feeds, 24 piglets of 20 days old were assigned to two ex...

Nowadays there is intense research interest to the polyphenol content of olive leaves which attributed significant antioxidant properties that may be related to diseases intake. The aim of this study was to assess the possible antioxidant action and antimutagenic effects of extracts from olive leaves. Encapsulation technology was also used, which a...

Grape pomace is a by-product of winemaking process and rich in bioactive compounds such as plant polyphenols having antioxidant and antimicrobial activities. As known, oxidative stress may cause a number of pathological conditions in farm animals and thus affecting animal welfare and production. Moreover, pathogenic bacteria affect animals’ health...

Aim: The purpose of this study was to examine the effects of a real water polo game on the oxidative stress and antioxidants markers of male water polo players. The importance of this study is that it is the first to examine the oxidative stress and antioxidants markers after a water polo game, considering the specific and high demands of the latt...

High Capacity Runner (HCR) rats have been developed by divergent artificial selection for treadmill endurance running capacity to explore an aerobic biology-disease connection. The beneficial effects of resveratrol supplementation have been demonstrated in endurance running and the antioxidant capacity of resveratrol is also demonstrated. In this s...

To investigate the effects of obesity and exercise training on plasma adipocytokines a sample of 42 children (lean = 24, %BF = 17.8 ± 7.5%; obese = 18; %BF = 29.1 ± 9.3%; mean age = 12.4 ± 1.9 yrs), were divided into 4 age-matched for activity groups: lean inactive (n = 11), obese inactive (n = 9), lean active (n = 13) and obese active (n = 9). Act...

Eupatorin-5-methyl ether (E5M) is a flavone containing 4 methoxy groups that is present in plants with medicinal activity, whereas luteolin (L) is a polyhydroxylated flavone commonly encountered in dietary products. In the present study we investigated the interaction of the two flavonoids with cytochrome P450 CYP1 enzymes in breast cancer MCF7 cel...

Spirulina is a popular nutritional supplement that is accompanied by claiMSS for antioxidant and performance-enhancing effects. Therefore, the aim of the present study was to examine the effect of spirulina supplementation on (i) exercise performance, (ii) substrate metabolism, and (iii) blood redox status both at rest and after exercise. Nine mode...

Effects of exercise training on important determinants of children's long-term health, such as redox and iron status, have not been adequately investigated. The aim of the present study was to examine changes in markers of the redox, iron and nutritional status of boy and girl swimmers during a prolonged period of training. 11 boys and 13 girls, ag...

The aim of this article is to present the effects of acute muscle-damaging exercise on oxidative stress/damage of animal and human tissues using a quantitative approach and focusing on the time-course of exercise effects. The reviewed studies employed eccentric contractions on a dynamometer or downhill running. The statistical power of each study t...

The effect of eccentric exercise on the time-course changes in several indices of muscle damage and blood oxidative stress was examined. Isometric torque, delayed-onset muscle soreness, creatine kinase, reduced glutathione (GSH), oxidized glutathione (GSSG), thiobarbituric-acid reactive substances (TBARS), protein carbonyls, catalase, uric acid, bi...

To examine the effect of repeated muscle-damaging exercise on the time-course changes in several indices of muscle damage, and to compare them with changes in blood oxidative stress indices. Twelve females underwent an isokinetic exercise session consisting of 75 lengthening knee flexions, which was repeated after 3 wk. Isometric torque, range of m...

To thoroughly investigate the time-course changes of several commonly used markers of oxidative stress by performing serial measurements during a 24-h period after an acute bout of strenuous cardiovascular exercise. Eleven untrained men performed two trials. In the experimental trial, the subjects exercised for 45 min at 70-75% VO2max and then at 9...

This study investigated the effect of an acute swimming protocol on selected blood redox status indices in trained children. Eleven boys and 11 girls (aged 911y) swam 12 bouts of 50m at a pace corresponding to 70%75% of the participants 50 m maximum velocity, with each bout separated by 1 min rest periods. At rest, no differences in any redox statu...

The blood redox status of child athletes is compared with that of age-matched untrained individuals. In the present study, 17 swimmers (10.1 +/- 1.6 years) and 12 non-athletes (9.9 +/- 1.1 years) participated. Reduced glutathione (GSH) was lower by 37% in swimmers compared to non-athletes (P < 0.01), oxidized glutathione (GSSG) was not different an...

This study was designed to investigate whether individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency can exercise without greater perturbations in their redox status compared with non-G6PD-deficient individuals. Nine males with established G6PD deficiency and nine males with normal G6PD activity performed two exhaustive treadmill exe...

A screening method for the detection of cardiotonic glycosides in horses urine is presented in this paper. This method is based on the detection by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-high resolution mass spectrometry (GC-HRMS) of the trimethylsilyl (TMS) derivatives of the aglycon moieties of these compounds after s...

In recent years it has become apparent that the oxidation of lipids, or lipid peroxidation, is a crucial step in the pathogenesis of several disease states in adult and infant patients. Lipid peroxidation is a process generated naturally in small amounts in the body, mainly by the effect of several reactive oxygen species (hydroxyl radical, hydroge...

Sex hormone binding globulin (SHBG) is a protein that circulates in the plasma of many species and binds with high affinity to sex steroids in the human and primates, while it is androgen-binding protein in many animals including sheep, dog, rabbit etc. Its expression is regulated by many hormones and recent evidence has shown that steroids regulat...

Papazisis KT, Kouretas D, Geromichalos GD, Sivridis E, Tsekreli OK, Dimitriadis KA, Kortsaris A.H. Effects of melatonin on proliferation of cancer cell lines. J. Pineal Res. 1998; 25:211–218. © Munksgaard, Copenhagen The pineal hormone melatonin has been reported to have in vitro antiproliferative effects on estrogen receptor-positive human breast...

RBL cells are not differentiated, but resemble mucosal mast cells (MMC). Two-dimensional (2-D) gel electrophoresis following isoelectric focusing (IEF) was performed using purified rat peritoneal mast cells and RBL cells. Certain similarities were identified with silver staining between mast cells and stationary phase (72 hr) RBL cells. RBL cells w...