Dimitra Chronaki

Dimitra Chronaki
Foundation for Research and Technology - Hellas | forth · Biology

PhD

About

6
Publications
763
Reads
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18
Citations
Introduction
Currently studying the interaction of proteins with surfaces using biophysical techniques such as acoustic biosensors, ellipsometry, calorimetry and infrared spectroscopy.
Education
November 2014 - October 2020
University of Crete / IMBB-FORTH
Field of study
  • Biosensors

Publications

Publications (6)
Article
Full-text available
In this work, the Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) was used to distinguish the dynamic cell adhesion behavior of human normal (Nthy) thyroid epithelial cells from poorly differentiated anaplastic carcinoma cells (ARO). The surfaces used to facilitate cell adhesion were bare titanium (Ti), gold (Au) and fibrinogen-coat...
Poster
Full-text available
The Type III secretion system (T3SS) is used by many Gram-negative pathogenic bacteria to deliver effector proteins directly into eukaryotic host cells (1). The T3SS machinery exports virulence factors from the cytoplasm through both the inner and the outer membrane to the external milieu. The proteins that are being secreted through this unique na...
Conference Paper
Full-text available
Preferred Presentation Method: Oral or Poster Introduction: The type III secretion system (T3SS) is a specialized bacterial protein secretory pathway that plays an essential role in the pathogenesis of Gram-negative bacteria (e.g. Enteropathogenic E. coli, EPEC) [1]. It is encoded by the Locus of Enterocyte Effacement (LEE), and injects effector pr...
Article
Full-text available
The present study demonstrates the sensitive and label-free acoustic detection of dsDNA amplicons produced from whole Salmonella Thyphimurium cells without employing any DNA extraction and/or purification step, in the presence of the lysed bacterial cells and in a hybridization-free assay. A sample-to-answer assay is also shown during DNA detection...

Questions

Question (1)
Question
I am currently using Native agarose gel electrophoresis to discriminate between 3 proteins of a different pI, so my running buffer is Tris-glycine (pH 8.5). Could you please suggest a running buffer of a different pH (preferably close to 6-7)?
Thank you all in advance

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Projects

Project (1)
Project
Our goal is to detect conformational changes of surface-immobilized proteins by using acoustic sensors.