Dieter G. Weiss

Dieter G. Weiss
Institut für Zelltechnologie IZT e.V.

Dr. rer. nat., Emer. Prof. for Animal Physiology

About

188
Publications
11,519
Reads
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5,289
Citations
Citations since 2016
6 Research Items
1379 Citations
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
Introduction
Axonal transport and other forms of intracellular motility; motor enzymes and cytoskeleton; methods to study those, especially live cell imaging
Additional affiliations
November 2013 - present
ZLWWG Zentrum für Logik, Wissenschftstheorie und Wissenschaftsgeschichte
Position
  • Board Member
Description
  • History and historical objects in cell biology and microscopy, paradigm shifts in life sciences
November 1997 - present
Institut für Zelltechnologie IZT e.V.
Position
  • CEO
Description
  • cell technology, advanced microscopy, live cell imaging for pharmacology and toxicology, in vitro testing, replacement of animal experimantation
March 1995 - September 1995
Dartmouth College
Position
  • Professor
Description
  • Harris Dartmouth German Visiting Professorship
Education
June 1982 - June 1982
Ludwig-Maximilians-University of Munich
Field of study
  • Cell Biology
April 1976 - April 1976
March 1968 - August 1968
University of Tuebingen
Field of study
  • Biology

Publications

Publications (188)
Article
Full-text available
Background Improving the neuronal yield from in vitro cultivated neural progenitor cells (NPCs) is an essential challenge in transplantation therapy in neurological disorders. In this regard, Ascorbic acid (AA) is widely used to expand neurogenesis from NPCs in cultures although the mechanisms of its action remain unclear. Neurogenesis from NPCs is...
Article
BCL-2 is a multifunctional protein involved in the regulation of apoptosis, cell cycle progression and neural developmental processes. Its function in the latter process is not well understood and needs further elucidation. Therefore, we characterized the protein expression kinetics of BCL-2 and associated regulatory proteins of the intrinsic apopt...
Conference Paper
Introduction: Phenotypic screening has led to the majority of successfully launched CNS drugs in the last 15 years. In vitro models using primary cultures are widely used for testing drug candidates. Here, the use of human induced pluripotent stem cell-derived (hiPSC) neurons is presented as an in vitro drug discovery assay of increased predictabil...
Article
Full-text available
Apoptosis is an essential physiological process accompanying the development of the central nervous system and human neurogenesis. However, the time scale and the underlying molecular mechanisms are yet poorly understood. Due to this fact, we investigated the functionality and general inducibility of apoptosis in the human neural ReNcell VM progeni...
Article
Full-text available
The present study was performed in an attempt to develop an in vitro integrated testing strategy (ITS) to evaluate drug-induced neurotoxicity. A number of endpoints were analyzed using two complementary brain cell culture models and an in vitro blood-brain barrier (BBB) model after single and repeated exposure treatments with selected drugs that co...
Article
Dynamic phase microscopy was used to study the dynamic events of formation of the endoplasmic reticulum (ER) in interphase-arrested Xenopus egg extract. We have shown that the ER periodically oscillated in an ATP-dependent manner in the frequency range of 1.6-2.2 Hz, while the tubular membrane network formed in vitro. The spectral density, i.e. the...
Article
Full-text available
Emerging evidence suggests that reactive oxygen species (ROS) can stimulate the Wnt/β-catenin pathway in a number of cellular processes. However, potential sources of endogenous ROS have not been thoroughly explored. Here, we show that growth factor depletion in human neural progenitor cells induces ROS production in mitochondria. Elevated ROS leve...
Article
It was suggested that gap junctional intercellular communication (GJIC) and connexin (Cx) proteins play a crucial role in cell proliferation and differentiation. However, the mechanisms of cell coupling in regulating cell fate during embryonic development are poorly understood. To study the role of GJIC in proliferation and differentiation, we used...
Article
Glycogen synthase kinase-3beta is a multifunctional key regulator enzyme in neural developmental processes and a main component of the canonical Wnt signaling pathway. It is already known that the Wnt-driven differentiation of neural progenitor cells is accompanied by an increase of apoptosis at which the pro-apoptotic function of GSK-3beta is stil...
Article
Since nano-sized particles (NPs) are increasingly used in various fields of innovative biomedicine and industrial technologies, it is of importance to identify their potential human health risk. We investigated whether ROS-induced mitochondrial DNA damage is the mode of action of titanium dioxide-NPs (TiO2-NPs; ≤20 nm) to induce cytotoxic and genot...
Article
A combined modality treatment employing radiation and chemotherapy plays a central role in the management of solid tumors. In our study, we examined the cytotoxic and radiosensitive effect of the microtubule stabilizer epothilone B on two human epithelial tumor cell lines in vitro and its influence on the microtubule assembly. Cancer cells were tre...
Article
Novel dermorphin tetrapeptides are described in which Tyr(1) is replaced by Dmt(1), where d-Ala(2) and Gly(4) are N-methylated, and where Phe(3)-Gly(4) residue is substituted by the constrained Aba(3)-Gly(4) peptidomimetic. Most of these peptidic ligands displayed binding affinities in the nanomolar range for both μ- and δ-opioid receptors but no d...
Article
Full-text available
ReNcell VM is an immortalized human neural progenitor cell line with the ability to differentiate in vitro into astrocytes and neurons, in which the Wnt/β-catenin pathway is known to be involved. However, little is known about kinetic changes of this pathway in human neural progenitor cell differentiation. In the present study, we provide a quantit...
Article
A series of analogues of conjugate 1, combining an adamantane-based paclitaxel (taxol) mimetic with colchicine was synthesized and tested for cytotoxicity in a cell-based assay with the human lung carcinoma cell line A549. The most active compounds (10 EC(50) 2 ± 1.0 nM, 23 EC(50) 6 ± 1.4 nM, 26 EC(50) 5 ± 1.8 nM, 28 EC(50) 11 ± 1.7 nM, 30 EC(50) 4...
Chapter
Neuronal networks cultured on microelectrode array (MEA) neurochips provide a testing platform for neuroactive compounds such as neurotoxicants and neuropharmaceuticals. Electrical network activity is recorded, quantified, characterized and classified at the level of spike and burst patterns, yielding concentration–response curves of a multitude of...
Article
Full-text available
Remodelling of the actin cytoskeleton plays a key role in particle internalisation and the phagosome maturation processes. Actin-binding proteins (ABPs) are the main players in actin remodelling but the precise role of these proteins in phagocytosis needs to be clarified. Annexins, a group of ABPs, are known to be present on phagosomes. Here, we id...
Article
Full-text available
Because of its diverse physical and chemical properties, lanthanum has been used in various industrial and medical fields. However, until recently, its effects at the cellular and molecular level had hardly been investigated. Using primary cortical networks grown on microelectrode array neurochips, we investigated the acute functional neurotoxicity...
Article
Full-text available
Background 3D-scaffolds have been shown to direct cell growth and differentiation in many different cell types, with the formation and functionalisation of the 3D-microenvironment being important in determining the fate of the embedded cells. Here we used a hydrogel-based scaffold to investigate the influences of matrix concentration and functional...
Article
Due to lack of knowledge only a few industrial chemicals have been identified as developmental neurotoxicants. Current developmental neurotoxicity (DNT) guidelines (OECD and EPA) are based entirely on in vivo studies that are both time consuming and costly. Consequently, there is a high demand to develop alternative in vitro methods for initial scr...
Article
The receptors engaged during recognition and phagocytic uptake of microorganisms and particles influence signaling events and diverse subcellular responses that occur during phagosome formation and maturation. However, pathogens generally have multiple ligands on their surface, making it difficult to dissect the roles of individual receptors during...
Article
Microelectrode arrays (MEAs) have been in use over the past decade and a half to study multiple aspects of electrically excitable cells. In particular, MEAs have been applied to explore the pharmacological and toxicological effects of numerous compounds on spontaneous activity of neuronal and cardiac cell networks. The MEA system enables simultaneo...
Article
A series of novel 1,3,4-oxadiazole derivatives based on structural and electronic overlap with combretastatins have been designed and synthesized. Initially, we tested all new compounds in vivo using the phenotypic sea urchin embryo assay to yield a number of agents with anti-proliferative, anti-mitotic, and microtubule destabilizing activities. Th...
Article
Full-text available
Nanomaterials are extensively used in industry and daily life, but little is known about possible health effects. An intensified research regarding toxicity of nanomaterials is urgently needed. Several studies have demonstrated that nanoparticles (NPs; diameter < 100 nm) can be transported to the central nervous system; however, interference of NPs...
Article
Full-text available
a b s t r a c t A series of novel 1,3,4-oxadiazole derivatives based on structural and electronic overlap with com-bretastatins have been designed and synthesized. Initially, we tested all new compounds in vivo using the phenotypic sea urchin embryo assay to yield a number of agents with anti-proliferative, anti-mitotic, and microtubule destabilizi...
Chapter
Functional screening of CNS drugs with neuronal network cultures on microelectrode arrays can elucidate different modes of action for a given substance at different concentrations. This concentration-dependent profiling of CNS drug candidates is a new feature of this technology. KeywordsFunctional screening–neuronal network cultures–microelectrode...
Article
Full-text available
Amyloid beta (Aβ) peptide deposits are one of the hallmarks of Alzheimer's disease (AD). For a long time it was believed that amyloid deposits in the senile plaques in the brain of patients with AD play a central role in the etiology of AD. Later many reports ascribed this role to different aggregates of Aβ (from fibrils, through high oligomers to...
Article
Four (2R,3S)-N-benzoylphenylisoserine esters with substituted bicyclo[3.3.1]nonanes are synthesized. The compounds are found to be unable to promote tubulin polymerization, but cause a weak tubulin aggregation (oligomerization).
Article
Nanotechnology presents countless opportunities to develop new and improved consumer products for the benefit of society. However, as the industrial production and use of nanotechnology products continue to expand at a fast scale, potential human health concerns and ecological safeguards for the environment need to be addressed. Health risk assessm...
Conference Paper
http://www.med.uni-rostock.de/medien-oeffentlichkeit/pressemitteilungen/aktuelles/news/neue-ansaetze-zur-behandlung-der-alzheimer-demenz-wissenschaftler-diskutieren-forschungserfolge-wae/
Conference Paper
http://www.albrecht-kossel-institut.de/en/5_events_news_links/past_events/springschool/5th_Stem_Cell_School/StemCellSchoolBooklet2008.pdf
Article
Several adamantane-based taxol mimetics were synthesized and found to be cytotoxic at micromolar concentrations and to cause tubulin aggregation. The extent of the aggregation is maximal for N-benzoyl-(2R,3S)-phenylisoseryloxyadamantane (5) and is very sensitive to the structural modifications. A hybrid compound (15), combining adamantane-based tax...
Article
Four specially designed bicyclo[3.3.1]nonane-based structures were synthesised and found to be cytotoxic at micromolar concentrations and to cause slight non-specific tubulin aggregation.
Article
Prediction of neurotoxic effects is a key feature in the toxicological profile of many compounds and therefore is required by regulatory testing schemes. Nowadays neurotoxicity assessment required by the OECD and EC test guidelines is based solely on in vivo testing, evaluating mainly effects on neurobehavior and neuropathology, which is expensive,...
Article
Full-text available
Interphase microtubules are organized into a radial array with centrosome in the center. This organization is a subject of cellular regulation that can be driven by protein phosphorylation. Only few protein kinases that regulate microtubule array in interphase cells have been described. Ste20-like protein kinase LOSK (SLK) was identified as a micro...
Conference Paper
In the age of systems biology there is a need for more quantitative data for the “omics” approaches. In parallel, quantitative image analysis and data generation are advancing and allow for the development of better models of cellular processes such as regulation of signalling networks, protein movements and remodelling of cellular 3D architecture....
Conference Paper
The mechanism of neural progenitor cell differentiation is not yet clearly understood. One mechanism of differentiation control is the activation of gene transcription by beta-catenin, a key player in the canonical Wnt-pathway, whereby gene activation depends on beta-catenin translocation into the nucleus. We quantified beta-catenin signals in diff...
Conference Paper
see meeting website: https://www.keystonesymposia.org/index.cfm?e=Web.Meeting.Program&MeetingID=926&pTime=ampm
Article
Full-text available
Amyloid beta (Aβ) peptides have been suggested to play an essential role in the generation of Alzheimer's disease (AD). In spite of the abundance of findings the exact correlation of Aβ and AD remains unclear. Aβ could influence cognition by impairment of basic neurotransmission mechanisms without causing directly neuronal death. This view is suppo...
Article
Traditional Parkinson's disease models in rats have several disadvantages. A promising alternative in terms of a more physiological model was proposed by McNaught et al. [McNaught, K.S., Perl, D.P., Brownell, A.L., Olanow, C.W., 2004. Systemic exposure to proteasome inhibitors causes a progressive model of Parkinson's disease. Ann. Neurol. 56, 149-...
Article
Full-text available
The synthesis and the results of biological testing of novel N-benzoylphenylisoserine-modified L-lupinine and L-menthol are reported.
Conference Paper
The canonical Wnt-pathway is known to be involved in the differentiation of neural progenitor cells of different origins (regions) and species. In order to investigate its complex regulatory network, we have conducted biochemical and microscopy analyses in a parallel and complementary fashion. We focussed on the major proteins involved in the canon...
Conference Paper
The canonical Wnt-pathway is involved in the differentiation of neural progenitor cells of different origins (regions) and species. In order to investigate its complex regulatory network, we use a combined approach of biochemical analyses, proteomics and microscopy studies, which are conducted in parallel and complement each other. Based on these d...
Conference Paper
The canonical Wnt-pathway is involved in the differentiation of neural progenitor cells of different origins (regions) and species [1]. Differentiation of neural progenitor cells is accompanied by a total reconstruction of the cell morphology. The development of neural extensions and branches like axons and dendrites is mediated by the reconstructi...
Article
Video contrast enhancement is a technique that considerably improves images obtained by a large variety of video-based imaging systems used in light, electron, and confocal microscopy. The specimens, most suitable for observation by this technique, are unstained, low-contrast objects, such as living cells, uncontrasted semi-thin electronmicroscopic...
Article
Full-text available
The possible harmful effects of radiofrequency electromag-netic fields (RF EMFs) are controversial. We have used hu-man Mono Mac 6 cells to investigate the influence of RF EMFs in vitro on cell cycle alterations and BrdU uptake, as well as the induction of apoptosis and necrosis in human Mono Mac 6 cells, using flow cytometry after exposure to a 18...
Article
Full-text available
The aim of the present study is to investigate whether extremely low frequency electromagnetic fields (ELF-EMF) affect certain cellular functions and immunologic parameters of mouse macrophages. In this study, the influence of 50 Hz magnetic fields (MF) at 1.0 mT was investigated on the phagocytic activity and on the interleukin-1beta (IL-1beta) pr...
Article
We optimized the novel technique of multielectrode neurochip recordings for the rapid and efficient screening of neuroactivity. Changes in the spontaneous activity of cultured networks of primary cortical neurons were quantified to evaluate the action of drugs on the firing dynamics of complex network activity. The multiparametric assessment of ele...