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Publications (34)
Common Palm Civet (Paradoxurus hermaphroditus) is a rare mammal appearing in South and Southeast Asia, first described in 1777. An expensive coffee is made of beans eaten and rejected by civets. In Vietnam, the animals are increasingly captured and kept captive to sell these beans, which has adversely affected their population. Because of the detri...
The second messenger cyclic adenosine 3′,5′-monophosphate (cAMP) synthesis is catalyzed by adenylyl cyclases (ACs). The second messenger cAMP participates in many physiological processes in cells. All eu-karyotic adenylyl cyclases (transmembrane adenylyl cyclase and soluble adenylyl cyclases) belong to class III. Soluble adenylyl cyclase was identi...
Research in the field of nanotechnologies, the development of reliable and environmentally friendly processes for the synthesis of metallic nanoparticles constitutes an important element in this field. In this study, biosynthesis of silver nanoparticles using Bacillus megaterium and Aspergillus niger is reported. The results show that the character...
The endocrine disruptors are mostly small organic molecules developed for numerous and very diverse industrial applications. They essentially act through nuclear receptors with small and hydrophobic endogenous ligands. Nevertheless, potential adverse effects through membrane hormone receptors cannot be ruled out, and have indeed been observed. The...
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors, which is also often fatal. An early and accurate diagnosis is a decisive step towards the survival of the patients. Molecular biology improved significantly the prognosis of liver cancers through learned use of tumor markers like proteantigens, cyto-kines, enzymes, isoenzyme...
Fluoxetine (FLX), a selective serotonin reuptake inhibitor antidepressant, exhibits various other mechanisms of action in numerous cell types and has been shown to induce cell death in cancer cells, paving the way for its potential use in cancer therapy. The aim of this study was to determine the off-target effects of the anti-depressant drug FLX,...
In contrast to all transmembrane adenylyl cyclases except ADCY9, the cytosolic soluble adenylyl cyclase (ADCY10) is insensitive to forskolin stimulation and is uniquely modulated by calcium and bicarbonate ions. In the present paper, we focus on ADCY10 localization and a kinetic analysis of intracellular cAMP accumulation in response to human LH in...
Cellular communications play pivotal roles in multi-cellular species, but they do so also in uni-cellular species. Moreover, cells communicate with each other not only within the same individual, but also with cells in other individuals belonging to the same or other species. These communications occur between two unicellular species, two multicell...
Cellular communications play pivotal roles in multi-cellular species, but they do so also in uni-cellular species. Moreover, cells communicate with each other not only within the same individual, but also with cells in other individuals belonging to the same or other species. These communications occur between two unicellular species, two multicell...
Cellular communications play pivotal roles in multi-cellular species, but they do so also in uni-cellular species. Moreover, cells communicate with each other not only within the same individual but also with cells in other individuals belonging to the same or other species. These communications occur between two unicellular species, two multicellu...
Adiponectin, an adipokine secreted by adipocytes, is a well-known homeostatic factor for regulating glucose levels, lipid metabolism, and insulin sensitivity through its anti-inflammatory, anti-fibrotic, and antioxidant effects. All these metabolic processes are mediated via two adiponectin receptors, AdipoR1 and AdipoR2. In addition, adiponectin i...
Cells communicate with other cells not only within the same individual but also with cells in other individuals belonging to the same species or other species. These communications occur between two unicellular species or two multicellular species, or between unicellular and multicellular species. The molecular mechanisms involved exhibit diversity...
Energy balance is an important feature for spermatozoa functions. The 5'-AMP-activated protein kinase (AMPK), a sensor of cell energy, has been implicated as a mediator between spermatozoa functions and energy balance. We recently identified and localized the AMPK protein in chicken spermatozoa and showed that its activation with non-specific activ...
Mouse Leydig Tumor cells (mLTC), transiently expressing cAMP-dependent luciferase, were used to study the influence of sexual steroids and of adiponectin (ADPN) on the cAMP response to luteinizing hormones (LH). While testosterone and progesterone had no significant effect, several molecules with estrogenic activity (17β-estradiol, ethynylestradiol...
Fluoxetine (FLX), a widely used antidepressant primarily acting as a selective serotonin reuptake inhibitor (SSRI), has been shown to exhibit other mechanisms of action in various cell types. Consequently, it might have unexpected adverse effects not related to its intended use, possibly in the endocrine regulation of reproduction. We show in the p...
Equine Chorionic Gonadotropin (eCG) previously known as Pregnant Mare Serum Gonadotropin (PMSG) has been used for decades in regulating reproduction in various domestic animal species. Its use necessitates a good measurement of its bioactivity in commercial preparations. The EUROPEAN PHARMACOPEIA (EP 7.0) recommends 5–6 subcutaneous injections in i...
Endocrine Disruptor Compounds (EDCs) are synthetic or natural molecules in the environment that promote adverse modifications of endogenous hormone regulation in humans and/or in wildlife animals. In the present paper, we review the potential mechanisms of EDCs and point out the similarities and differences between EDCs and hormones. There was only...
Semen cryopreservation allows crucial management of animal genetic diversity. However, the freeze-thaw process causes biochemical and physical alterations, impairing sperm energy-dependent functions. Currently, many chemicals are added to the media to enhance frozen-thawed sperm quality during artificial insemination. The aims of this study was to...
AMP-activated protein kinase (AMPK) is a key enzyme involved in linking the energy sensing to metabolic pathways. As such, it plays a central role at the whole-body level to translate endocrine communications into adapted responses aimed either at saving energy when food is scarce or at allocating it to various functions, particularly reproduction,...
Motility is an essential function of the mature male gamete and is widely used as an indicator of semen quality. Knowledge of signal transduction pathways regulating sperm motility is therefore important to gain insight into new methods to increase the fertilizing ability of semen samples, especially those subjected to biotechnological processes su...
Fluoxetine (Prozac), a selective Serotonin Reuptake Inhibitor antidepressant, exhibits other mechanisms of action in various cell types and has been shown to induce cell death in cancer cells, paving the way for its potential use in cancer therapy. The ovary is a complex endocrine organ responsible for steroidogenesis and folliculogenesis, and huma...
As we already know, the male reproductive system requires less energetic investment than the female one. Nevertheless, energy balance is an important feature for spermatozoa production in the testis and for spermatozoa properties after ejaculation. The 5'-AMP-activated protein kinase, AMPK, is a sensor of cell energy, that regulates many metabolic...
This book provides students (sciences, medicine, pharmacy) with the bases of cellular biology and cellular biochemistry. Both prokaryotes and eukaryotes are taken into consideration and among the latter, both uni-cellular and multi-cellular organisms. This text is aimed at giving essential information concerning the molecular composition, structure...
In order to study the intracellular cAMP response kinetics of Leydig cells to hormones with LH activity, we used MLTC-1 cells transiently expressing a chimeric cAMP-responsive luciferase so that real-time variations of intracellular cAMP concentration could be followed using oxiluciferin luminescence produced from catalyzed luciferin oxidation. The...
The 5'-AMP-activated protein kinase, AMPK, is a key protein kinase in the metabolism of the cell that regulates many metabolic pathways. The involvement of cell metabolism in sperm ability to fertilize is well established, but only a few studies have focused on the role of AMPK in the control of male fertility. This article summarizes the known rol...
Intracellular cytoplasmic calcium ([Ca(2+) ]i ) has an important regulatory role in gamete functions. The involved biochemical actors, however, are still unknown in birds, an animal class that has lost functional sperm-specific CatSper channels. In this report, we provide evidence for the presence and expression of different Ca(2+) channels in chic...
Sperm require high levels of energy to ensure motility and acrosome reaction (AR) accomplishment. The AMP-activated protein kinase (AMPK) has been demonstrated to be strongly involved in the control of these properties. We address here the question of the potential role of calcium mobilization on AMPK activation and function in chicken sperm throug...
Semen cryopreservation is a unique tool for the management of animal genetic diversity. However, the freeze-thaw process causes biochemical and physical alterations which make difficult the restoration of sperm energy-dependent functions needed for fertilization. 5'-AMP activated protein kinase (AMPK) is a key sensor and regulator of intracellular...
Avian gametes present specific features related to their internal long-term mode of fertilization. Among other central actors of energetic metabolism control, 5'-AMP-activated protein kinase (AMPK) has been suspected to influence sperm functions and thus to play a key role in fertilization success. In the present work, we studied AMPK localization...
Semen cryopreservation is a key tool to manage the propagation of genetic potential of males and the genetic diversity. However the freeze-thaw process cause biochemical and physical alterations including alteration in the energetic metabolism that make difficult the restoration of sperm energetic dependent functions needed to fertilization such as...
Spermatozoa are highly specialized cells whose main properties include motility and acrosome reaction. These processes require a high level of energy related to the intracellular ATP / 5’AMP ratio. AMPK (5’-AMP activated protein kinase) is a sensor of this ratio and its numerous roles include regulation of glucose, lipid, and protein metabolisms. W...
The sperm requires energy for specific functions which are mainly to reach and fertilize the ovum. The objective of the present study is to clarify the role of an energy sensor, AMPK, by studying the effects of specific AMPK inhibitors (1μM, 5μM and 10μM Compound C and 0.1mM and 0.6mM ARA-A) on sperm motility, viability and acrosome reaction compar...
