Diego Santos-Garcia

Diego Santos-Garcia
Claude Bernard University Lyon 1 | UCBL · Laboratoire de biométrie et biologie evolutive (LBBE)

Biology, M. Sc. in Genetics, Ph. D.
Marie Skłodowska-Curie Individual Fellow

About

103
Publications
23,289
Reads
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Introduction
The rising global population puts constant pressure on food production and intensive agricultural practices. Alongside the injudicious use of insecticides, this has modified agrarian ecosystems, causing more plant vector-borne viral diseases than ever before. The EU-funded GuardSym project will focus on the whitefly Bemisia tabaci as a vector to investigate its multitrophic interactions with its bacterial symbionts, vectored virus, and host plants.
Additional affiliations
February 2021 - present
French National Centre for Scientific Research
Position
  • PostDoc Position
May 2019 - January 2021
French National Centre for Scientific Research
Position
  • PostDoc Position
January 2015 - March 2019
Hebrew University of Jerusalem
Position
  • PostDoc Position
Education
January 2016 - April 2016
The Linux Foundation (edX)
Field of study
  • Linux
March 2014 - April 2014
Coursera - Prof. Nicholas Provart (University of Toronto)
Field of study
  • Bioinformatics (12 h)
February 2014 - March 2014
Coursera - Prof. Nicholas Provart (University of Toronto)
Field of study
  • Bioinformatics (12 h)

Publications

Publications (103)
Article
Full-text available
Whiteflies are important agricultural insect pests, whose evolutionary success is related to a long-term association with a bacterial endosymbiont, Candidatus Portiera aleyrodidarum. To completely characterize this endosymbiont clade, we sequenced the genomes of three new Portiera strains covering the two extant whitefly subfamilies. Using endosymb...
Article
Full-text available
Many insect endosymbionts described so far are gram-negative bacteria. Primary endosymbionts are obligatory bacteria usually harboured by insects inside vacuoles in specialized cells called bacteriocytes. This combination produces a typical three-membrane system with one membrane derived from the insect vacuole and the other two from the bacterial...
Article
Full-text available
Moss bugs (Coleorrhyncha: Peloridiidae) are members of the order Hemiptera, and like many hemipterans, they have symbiotic associations with intracellular bacteria to fulfil nutritional requirements resulting from their unbalanced diet. The primary endosymbiont of the moss bugs, Candidatus Evansia muelleri, is phylogenetically related to Candidatus...
Article
Full-text available
Many insects harbour inherited bacterial endosymbionts. Although some of them are not strictly essential and are considered facultative, they can be a key to host survival under specific environmental conditions, such as parasitoid attacks, climate changes or insecticide pressures. The whitefly Bemisia tabaci is top of the list of organisms inflict...
Article
Full-text available
The genome of “Candidatus Portiera aleyrodidarum,” the primary endosymbiont of the whitefly Bemisia tabaci (Mediterranean species), is reported. It presents a reduced genome (357 kb) encoding the capability to synthetize, or participate in the synthesis of, several amino acids and carotenoids, being the first insect endosymbiont capable of supplyin...
Article
Full-text available
Although generalist insect herbivores can migrate and rapidly adapt to a broad range of host plants, they can face significant difficulties when accidentally migrating to novel and marginally-suitable hosts. What happens, both in performance and gene expression regulation, if these marginally-suitable hosts must be used for multiple generations bef...
Article
Full-text available
Leprosy is a dreaded infection that still affects millions of people worldwide. Mycobacterium lepromatosis is a recently recognized cause in addition to the well-known Mycobacterium leprae . M. lepromatosis is likely specific for diffuse lepromatous leprosy, a severe form of the infection and endemic in Mexico. This study constructed and annotated...
Article
Metabolic conversions allow organisms to produce essential metabolites from the available nutrients in an environment, frequently requiring metabolic exchanges among co-inhabiting organisms. Here, we applied genomic-based simulations for exploring tri-trophic interactions among the sap-feeding insect whitefly (Bemisia tabaci), its host-plants, and...
Data
Supplementary Information for "Glucosylation prevents plant defense activation in phloem-feeding insects"
Article
Full-text available
The whitefly Bemisia tabaci is a closely related group of >35 cryptic species that feed on the phloem sap of a broad range of host plants. Species in the complex differ in their host‐range breadth, but the mechanisms involved remain poorly understood. We investigated, therefore, how six different B. tabaci species cope with the environmental unpred...
Article
Full-text available
The metabolic adaptations by which phloem-feeding insects counteract plant defense compounds are poorly known. Two-component plant defenses, such as glucosinolates, consist of a glucosylated protoxin that is activated by a glycoside hydrolase upon plant damage. Phloem-feeding herbivores are not generally believed to be negatively impacted by two-co...
Poster
Full-text available
Whiteflies (Hemiptera: Sternorrhyncha: Aleyrodidae) are a superfamily of small phloem-feeding insects. They rely on their primary endosymbiont, Candidatus Portiera aleyrodidarum, to obtain essential amino acid not present in their diet. Portiera has been co-diverging with whiteflies since their origin and therefore reflects their host evolutionary...
Article
Full-text available
Whiteflies (Hemiptera: Sternorrhyncha: Aleyrodidae) are a superfamily of small phloem-feeding insects. They rely on their primary endosymbionts Candidatus Portiera aleyrodidarum to produce essential amino acids not present in their diet. Portiera has been co-diverging with whiteflies since their origin and therefore reflects its host’s evolutionary...
Preprint
Full-text available
Whiteflies (Hemiptera: Sternorrhyncha: Aleyrodidae) are a superfamily of small phloem-feeding insects. Their taxonomy is currently based on the morphology of nymphal stages that display phenotypic plasticity, which produces inconsistencies. To overcome this limitation, we developed a new phylogenetic framework that targets five genes of Candidatus...
Article
Full-text available
The glutathione S-transferase (GST) family plays an important role in the adaptation of herbivorous insects to new host plants and other environmental constrains. The family codes for enzymes that neutralize reactive oxygen species and phytotoxins through the conjugation of reduced glutathione. Here, we studied the molecular evolution of the GST fa...
Article
Full-text available
The glutathione S-transferase (GST) family plays an important role in the adaptation of herbivorous insects to new host plants and other environmental constrains. The family codes for enzymes that neutralize reactive oxygen species and phytotoxins through the conjugation of reduced glutathione. Here, we studied the molecular evolution of the GST fa...
Article
Full-text available
While most insect herbivores are selective feeders, a small proportion of them feed on a wide range of plants. This polyphagous habit requires overcoming a remarkable array of defenses, which often necessitates an adaptation period. Efforts for understanding the mechanisms involved mostly focus on the insect’s phenotypic plasticity. Here, we hypoth...
Article
Full-text available
A Dyella-like bacterium was previously isolated from the planthopper Hyalesthes obsoletus (Hemiptera). Based on its 16S rRNA gene sequence, strain DHo T was assigned to the family Rhodanobacteraceae with Dyella and Frateuria as its closest relatives. The closest 16S rRNA gene sequences were Frateuria aurantia DSM 6220 T (98.2 %), Dyella thiooxydans...
Article
Full-text available
Candidatus Liberibacter solanacearum (Lso) haplotype D (LsoD) is a suspected bacterial pathogen, spread by the phloem-feeding psyllid Bactericera trigonica Hodkinson and found to infect carrot plants throughout the Mediterranean. Haplotype D is one of six haplotypes of Lso that each have specific and overlapping host preferences, disease symptoms,...
Article
Full-text available
Insect lineages feeding on nutritionally restricted diets such as phloem sap, xylem sap, or blood, were able to diversify by acquiring bacterial species that complement lacking nutrients. These bacteria, considered obligate/primary endosymbionts, share a long evolutionary history with their hosts. In some cases, however, these endosymbionts are not...
Article
Full-text available
Insect‐plant associations and their role in diversification are mostly studied in specialists. Here, we aimed to identify macroevolution patterns in the relationships between generalists and their host plants that have the potential to promote diversification. We focused on the Bemisia tabaci species complex containing more than 35 cryptic species....
Article
Full-text available
Background: Individual organisms are linked to their communities and ecosystems via metabolic activities. Metabolic exchanges and co-dependencies have long been suggested to have a pivotal role in determining community structure. In phloem-feeding insects such metabolic interactions with bacteria enable complementation of their deprived nutrition....
Data
Linux cheatsheet as a mouse pad template. Can be modified with any Vector Graphics Software. InkScape is an open source alternative.
Preprint
Full-text available
Insect lineages feeding on nutritionally restricted diets such as phloem, xylem, or blood, were able to diversify by acquiring bacterial species that complemented the missing nutrients. These bacteria, considered obligate/primary endosymbionts, share a long evolutionary history with their hosts. In some cases, however, these endosymbionts are not a...
Article
Full-text available
Hemipteran insects are well-known in their ability to establish symbiotic relationships with bacteria. Among them, heteropteran insects present an array of symbiotic systems, ranging from the most common gut crypt symbiosis to the more restricted bacteriome-associated endosymbiosis, which have only been detected in members of the superfamily Lygaeo...
Article
Full-text available
Many insects rely on bacterial endosymbionts to obtain nutrients that are scarce in their highly specialized diets. The most surprising example corresponds to the endosymbiotic system found in mealybugs from subfamily Pseudococcinae in which two bacteria, the betaproteobacterium /`Candidatus Tremblaya princeps/' and a gammaproteobacterium, maintain...
Article
Full-text available
We report the genome sequence of Exiguobacterium chiriqhucha str. N139, isolated from a high-altitude Andean lake. Comparative genomic analyses of the Exiguobacterium genomes available suggest that our strain belongs to the same species as the previously reported E. pavilionensis str. RW-2 and Exiguobacterium str. GIC 31. We describe this species a...
Data
rRNA copy numbers in E. chiriqhucha str. N139 and E. sp. S17. r ibosomal operons are shown in the gel, obtained by digesting the whole genome with enzyme I-Ceu l, as described in the Supplementary Methods. This enzyme binds to a 23 bp-sequence located in the middle of the 23S rRNA gene. The gel was exposed 0.5s to UV light. StLT2 corresponds to Sal...
Data
Clade II Strain Specific Genes through their COG categories The pangenome of all the complete genome sequences of the Exiguobacterium genus from clade II was calculated with the OrthoMCL pipeline (Li, Jr & Roos, 2003; Fischer et al., 2011). From the pangenome, the core genome was calculated, as well as the Strain Specific Genes (SSGs). These SSGs c...
Data
Megaplasmids in Exiguobacterium chiriqhucha str. N139 and E. sp. S17 (1) Weight molecular marker 50–100 kb; (2) N139; (3) S17; (4) Weight molecular marker 0.1–200 kb; (5) E. coli 3496. Three megaplasmids are observed in the Exiguobacterium strains from HAAL of sizes 250. 57, 137.48 and 48 kb in E. chiriqhucha. str. N139; and 251.98, 140.8 and 47.94...
Preprint
Full-text available
Individual organisms are linked to their communities and ecosystems via metabolic activities. Metabolic exchanges and co-dependencies have long been suggested to have a pivotal role in determining community structure. Metabolic interactions with bacteria have been key drivers in the evolution of sap-feeding insects, enabling complementation of thei...