Diego Rubiales

Diego Rubiales
Spanish National Research Council | CSIC · Institute for Sustainable Agriculture (IAS)

Professor

About

562
Publications
159,772
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
16,270
Citations
Introduction
Full Professor at the Inst. for Sustainable Agriculture, CSIC, at Córdoba, Spain. Research interest in legume and cereal breeding for disease resistance. Has published 353 IF-articles, reaching a h-index=54. He has supervised 30 PhDs. He has been President of the International Legume Society and of the European one, and member of the Ex.Com of the Int. Parasitic Plant Society. He has extensive experience in coordination of research (18 international and 18 Spanish projects).
Additional affiliations
April 2006 - November 2016
Spanish National Research Council
Position
  • Professor
June 2003 - April 2006
Spanish National Research Council
Position
  • Senior Researcher
January 1993 - May 2003
Spanish National Research Council
Position
  • Tenured Scientist

Publications

Publications (562)
Article
Full-text available
Aphanomyces root rot (ARR) is a devastating disease that affects the production of pea. The plants are prone to infection at any growth stage, and there are no chemical or cultural controls. Thus, the development of resistant pea cultivars is important. Phenomics technologies to support the selection of resistant cultivars through phenotyping can b...
Article
Full-text available
Multi-environment field testing of chickpea accessions winter sown in Southern Spain showed that environmental effects on yield were more important than genotypic effects and GEI. The most detrimental factor on grain yield was ascochyta blight infection. We did not find a significant effect of low temperatures on yield in the environments studied,...
Article
Full-text available
Pea rust is a major disease worldwide caused by Uromyces pisi in temperate climates. Only moderate levels of partial resistance against U. pisi have been identified so far in pea, urging for enlarging the levels of resistance available for breeding. Herein, we describe the responses to U. pisi of 320 Pisum spp. accessions, including cultivated pea...
Article
Full-text available
Sulla is a biannual forage legume cultivated throughout the Mediterranean Basin. It can be severely damaged by powdery mildew, but there is little understanding on its causal species or its host range. The taxonomic characterization of Erysiphe species is mainly based on the examination of chasmothecia morphology, or on the molecular analysis of IT...
Article
Full-text available
“Desert hyacinths” are a remarkable group of parasitic plants belonging to genus Cistanche, including more than 20 accepted species typically occurring in deserts or coastal dunes parasitizing roots of shrubs. Several Cistanche species have long been a source of traditional herbal medicine or food, being C. deserticola and C. tubulosa the most used...
Article
Full-text available
The faba bean (Vicia faba) is a temperate grain legume, that is regaining interest due to the high demand for food and feed uses and the environmental services provided. The parasitic weed broomrape (Orobanche crenata) appears as the major constraint to agricultural production in the Mediterranean Basin. The yield stability can be managed by adjust...
Article
Full-text available
Grass pea (Lathyrus sativus L.) is an annual cool-season grain legume widely cultivated in South Asia, Sub-Saharan Africa, and in the Mediterranean region. It is a stress-resilient crop with high nutritional value, considered a promising source of traits to breed for adaptation/mitigation of climate change effects. It is also reported as a suitable...
Article
Background: Rusts and powdery mildews are severe fungal diseases of major crops worldwide, including cereals and legumes. They can be managed by chemical fungicide treatments, with negative consequences as environmental pollution and risk for human and animal health. Bioactive natural products could be a safest alternative for pest control. The fa...
Article
Full-text available
Context. Yield losses in lentil (Lens culinaris ssp. culinaris Medik.) caused by weeds are estimated at 20–80%. In the absence of effective broad-spectrum herbicides, the menace of weed has emerged as a serious yield constraint in lentil. Aims. Identification of tolerance to two broad-spectrum post- emergence herbicides, imazethapyr and metribuzin....
Article
Full-text available
Rusts are among the most important foliar biotrophic fungal diseases in legumes. Lathyrus cicera crop can be severely damaged by Uromyces pisi, to which partial resistance has been identified. Nevertheless, the underlying genetic basis and molecular mechanisms of this resistance are poorly understood in L. cicera. To prioritise the causative varian...
Article
Full-text available
Uromyces pisi ([Pers.] D.C.) Wint. is an important foliar biotrophic pathogen infecting grass pea (Lathyrus sativus L.), compromising their yield stability. To date, few efforts have been made to assess the natural variation in grass pea resistance and to identify the resistance loci operating against this pathogen, limiting its efficient breeding...
Article
Full-text available
Crop wild relatives are species related to cultivated plants, whose populations have evolved in natural conditions and confer them valuable adaptive genetic diversity, that can be used in introgression breeding programs. Targeting four wild lentil taxa in Europe, we applied the predictive characterization approach through the filtering method to id...
Article
Full-text available
Faba bean (Vicia faba) is a grain legume crop widely cultivated in temperate areas for food and feed. Its productivity can be constrained by numerous diseases and pests that can be managed by a number of strategies, complemented with the deployment of resistant cultivars in an integrated manner. Few sources of resistance are available to some of th...
Article
Full-text available
Intercropping of two or more species on the same piece of land can enhance biodiversity and resource use efficiency in agriculture. Traditionally, intercropping systems have been developed and improved by empirical methods within a specific local context. To support the development of promising intercropping systems, the individual species that are...
Article
Full-text available
E. pisi was thought to be the only causal agent of powdery mildew in peas, with three genes, er1, er2 and Er3, conferring resistance to this pathogen. Recently, E. trifolii has also been found to cause this disease in peas in different countries, but its relevance in pea powdery mildew disease worldwide is unknown. The objective of this study was t...
Article
Full-text available
The adaptability and stability of 37 faba bean (Vicia faba L.) accessions with different levels of tolerance to metribuzin or imazethapyr was assessed across 12 season–location–herbicide experiments. Significant Genotype x environment (GE) interaction was found for the days to flowering (DFLR), plant height (PLHT) and grain yield (GY). Performance...
Article
Full-text available
Weeds represent one of the major constraints for faba bean crop. The identification of molecular markers associated with key genes imparting tolerance to herbicides can facilitate and fasten the efficient and effective development of herbicide tolerant cultivars. We phenotyped 140 faba bean genotypes in three open field experiments at two locations...
Chapter
Faba bean (Vicia faba L.) was first domesticated in the Fertile Crescent of the Near East. It ranks fourth among the cool season food legumes and is now grown in more than 66 countries. Faba bean is a versatile crop; besides being an important source of protein for food and feed, it offers many services for the ecosystem. The Mediterranean and East...
Article
Full-text available
Common bean is a nutritious food legume widely appreciated by consumers worldwide. It is a staple food in Latin America, and a component of the Mediterranean diet, being an affordable source of protein with high potential as a gourmet food. Breeding for nutritional quality, including both macro and micronutrients, and meeting organoleptic consumers...
Article
Full-text available
Wider and more profitable legume crop cultivation is an indispensable step for the agroecological transition of global agri-food systems but represents a challenge especially in Europe. Plant breeding is pivotal in this context. Research areas of key interest are represented by innovative phenotypic and genome-based selection procedures for crop yi...
Article
Full-text available
Chocolate spot, which is caused by the necrotrophic fungus Botrytis fabae, is a major foliar disease occurring worldwide and dramatically reducing crop yields in faba bean (Vicia faba). Although chemical control of this disease is an option, it has serious economic and environmental drawbacks that make resistant cultivars a more sensible choice. Th...
Article
Full-text available
Grass pea (Lathyrus sativus L.) is an annual legume species, phylogenetically close to pea (Pisum sativum L.), that may be infected by Fusarium oxysporum f. sp. pisi (Fop), the causal agent of fusarium wilt in peas with vast worldwide yield losses. A range of responses varying from high resistance to susceptibility to this pathogen has been reporte...
Article
Full-text available
There is potential for expanding lentil cultivation to dry and warm Mediterranean rain-fed environments at low altitudes, where early sowings are recommended to profit from winter rains and escape drought and excessive heat at the grain filling stage. In cooler areas, frost might be a problem in the early sowings, however, in warmer areas such as o...
Article
Metabolomics is one of the most powerful -omics to assist plant breeding. Despite the recognized genetic diversity in Portuguese common bean germplasm, details on its metabolomics profiles are still missing. Aiming to promote their use and to understand the environment’s effect in bean metabolomics profiles, 107 Portuguese common bean accessions, c...
Article
Full-text available
Common bean ( Phaseolus vulgaris L.), one of the most consumed food legumes worldwide, is threatened by two main constraints that are found frequently together in nature, water deficit (WD) and fusarium wilt ( Fop ). To understand the shared and unique responses of common bean to Fop and WD, we analyzed the transcriptomic changes and phenotypic res...
Article
Full-text available
Purpose Grass pea is a legume species with recognized resistance to several diseases and thus important for the improvement of related major legume crops. It is infected by the soil-borne fungus Fusarium oxysporum f. sp. pisi (Fop), known as causal agent of pea fusarium wilt. We aim to identify, among grass pea, new sources of resistance against Fo...
Article
Full-text available
Powdery mildews are major diseases for a range of crops. The loss of function of specific Mildew Locus O (MLO) genes has long been associated with pre-haustorial plant resistance to powdery mildew and has proven to be durable in several species. Erysiphe pisi is the major causal agent of powdery mildew in pea (Pisum sativum L.) and in the closely r...
Article
Faba bean (Vicia faba) is a temperate grain legume of major importance in Mediterranean agriculture. Faba bean production can be severely constrained in the area by rust incited by Uromyces viciae-fabae. Control with fungicides is possible, but there is a growing interest on alternative control methods, compatible with organic agriculture. Intercro...
Article
Multi service cover crops are used to provide ecosystem services, particularly for nitrogen management, such as “nitrate catching” and "green manuring" effects Sowing cover crop mixtures including legumes and non legumes have the advantage of combining the provision of both services related to N management thanks to phenomena of niche complementari...
Article
Full-text available
In Mediterranean environments, with mild winters and dry summers, peas are planted in autumn or early winter to profit from winter rain and to avoid terminal drought and high summer temperatures. The root parasitic weed broomrape (Orobanche crenata) appears as a major limiting factor under these conditions. To address such specific growing conditio...
Article
Full-text available
Pea is a temperate grain legume cultivated worldwide that can be severely constrained by powdery mildew infection. Control by fungicides and the use of resistant cultivars is possible, but there is a growing interest in alternative control methods such as crop diversification, particularly in low input agriculture. The aim of this work was to asses...
Article
Full-text available
Leaf rust and powdery mildew are two important foliar diseases in wheat. A recombinant inbred line (RIL) population, obtained by crossing two bread wheat cultivars (‘Victo’ and ‘Spada’), was evaluated for resistance to the two pathogens at seedling stage. Upon developing a genetic map of 8726 SNP loci, linkage analysis identified three resistance Q...
Article
Full-text available
The intercropping of two or more crop species on the same piece of land at a given time has been hypothesized to enhance crop yield stability. To address this hypothesis, we assessed the grain yield stability of various barley-pea and wheat-faba bean mixtures grown in seven experimental field trials (locations) across Europe during two years with c...
Article
Vascular wilt, caused by the infection of the soil-borne pathogen Fusarium oxysporum (Fo), is one of the most destructive diseases of many crops, including legumes such as grass pea (Lathyrus sativus), with several formae speciales (ff. spp.) defined according to their hosts. Commonly described as host-specific, Fo could, in some cases, show a broa...
Article
Full-text available
Pea (Pisum sativum L.), a cool-season legume crop grown in more than 85 countries, is the second most important grain legume and one of the major green vegetables in the world. While pea was historically studied as the genetic model leading to the discovery of the laws of genetics, pea research has lagged behind that of other major legumes in the g...
Article
Full-text available
Weeds cause serious constraint to faba bean productivity. Broad weed control is hampered by the availability of post‐emergence herbicides to control them, as the current faba bean cultivars are highly susceptible to them. Therefore, the deployment of post‐emergence herbicide tolerance is desirable in faba bean. To address this, 130 accessions were...
Article
Full-text available
The common bean (Phaseolus vulgaris L.) represents a sustainable and affordable source of protein, namely, to populations with vegetarian dietary habits. Despite the national germplasm genetic diversity, little is known about the Portuguese accessions’ nutritional and protein quality, leading to their underuse in breeding programs. To fill this gap...
Chapter
This chapter addresses advances in understanding plant root responses to weedy root parasites. It begins by reviewing host-parasitic weed interactions, focusing specifically on seed dispersal and germination and the possibility of host infection as a consequence of germination. The chapter then moves on to discuss host plant pre-penetration and pos...
Article
Full-text available
There is current interest in the revalorization of legume crops such as one-flowered vetch (Vicia articulata) that was widely cultivated in the past, but is today underutilized. Characterization of responses of existing germplasm to major biotic constraints is crucial for cultivar development. Our studies confirm that V. articulata germplasm can be...
Chapter
Full-text available
Faba bean is a cool-season annual grain legume that originated in the southwestern Asia. It is now grown on all continents but on only 2.5 Mha when compared with 5.5 Mha in 1960. The largest production is in Mediterranean environments, but it is also grown in high altitude subtropical environments and extends to cold-temperate areas. Faba bean is a...
Article
Full-text available
Pea (Pisum sativum) is an important grain legume worldwide whose cultivation is severely constrained by the root parasitic weed crenate broomrape (Orobanche crenata), which is widespread in the Mediterranean Basin and Middle East. No resistance is available in commercialized cultivars but some levels of incomplete resistance has been reported in pe...
Technical Report
Full-text available
Growing two or more plant species in close proximity (‘plant teams’) is a practice that can be adopted to improve crop production in terms of increased productivity, more efficient resource use, reduced reliance on crop protection chemicals, and biodiversity enhancement. Here, we summarise findings from experimental trials conducted across Europe t...
Article
Full-text available
Grass pea (Lathyrus sativus) is an annual legume crop that is currently underutilized but has the potential for reintroduction into Mediterranean rain-fed farming systems. In this study, we compared the adaptation of breeding lines in multi-environment field testing, which had wide variation for precocity, grain yield and broomrape infection. Herit...
Article
Full-text available
Anthracnose, caused by the fungal pathogen Colletotrichum lentis, is a severe disease of lentil (Lens culinaris) causing premature defoliation, necrotic stem lesions that lead to plant wilting and death in susceptible varieties. Two races of C. lentis (0 and 1) have been described so far. Race 0 is the most virulent one and limited genetic resistan...
Article
Full-text available
Legume species are recognized for their nutritional benefits and contribution to the sustainability of agricultural systems. However, their production is threatened by biotic constraints with devastating impacts on crop yield. A deep understanding of the molecular and genetic architecture of resistance sources culminating in immunity is critical to...
Article
Full-text available
Grass pea (Lathyrus sativus) is an annual legume crop widely cultivated in South Asia and Sub-Saharan Africa, but in regression in Mediterranean region. Its rusticity and nutritious value is calling back attention for its reintroduction into Mediterranean rain-fed farming systems. We studied the adaptation of a range of breeding lines in multi-envi...
Article
Full-text available
Legumes are among the most important crops worldwide for human and animal consumption. However, yield inconsistency due to susceptibility to pests and diseases strongly affects its production. Among diseases affecting legumes, Fusarium wilt caused by the soil-borne pathogen Fusarium oxysporum Schltdl. (Fo) is one of the major factors limiting produ...
Preprint
Full-text available
Lentil (Lens culinaris Medik.) is cultivated under a wide range of environmental conditions, which led to diverse phenological adaptations and resulted in a decrease in genetic variability within breeding programs due to reluctance in using genotypes from other environments. We phenotyped 324 genotypes across nine locations over three years to asse...
Chapter
As sessile organisms, plants are constantly exposed to simultaneously abiotic and biotic stresses that impact growth thus resulting in significant yield losses. An example is drought and root infecting pathogens, which combined cause greater damage to plants than the stresses individually. Substantial information is available on the physiological,...
Article
The broomrapes are plants that have modified their biology to feed on roots of other plants, emerging above the soil only to flower. There are about 150 broomrape species, most of which infect wild plants in natural habitats without causing economic problems. However, a few of them have adapted to agricultural ecosystems becoming troublesome root p...
Article
Powdery mildews infections are among the most severe foliar biotrophic fungal diseases in grain legumes. Several accessions of Lathyrus cicera (chickling pea) show levels of partial resistance to Erysiphe pisi, the causal agent of pea powdery mildew, and to E. trifolii, a powdery mildew pathogen recently confirmed to infect pea and Lathyrus spp. Ne...
Article
Full-text available
One-flower vetch (Vicia articulata) was widely cultivated in the Mediterranean Basin in the past but is currently underutilized. Valuable germplasm collections are stored in genebanks, which are poorly characterized. In an attempt to explore adaptation of landraces we performed a multi-environment field testing, showing the availability of valuable...
Article
Peyronellaea pinodes causes Ascochyta blight, one of the major diseases in pea worldwide. Cultivated pea plants have a low resistance to this disease. Although quantitative trait loci (QTLs) involved in the resistance to Ascochyta blight have been identified, the specific genes associated with these QTLs remain unknown, which makes marker-assisted...
Article
Full-text available
Common bean (Phaseolus vulgaris L.) is one of the most consumed legume crops in the world and fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. phaseoli (Fop), is one of the major diseases affecting its production. Portugal holds a very promising common bean germplasm with an admixed genetic background that may reveal novel genetic resi...
Article
Full-text available
Pea weevil (Bruchus pisorum) is a damaging insect pest affecting pea (Pisum sativum) production worldwide. No resistant cultivars are available, although some levels of incomplete resistance have been identified in Pisum germplasm. To decipher the genetic control underlying the resistance previously identify in P. sativum ssp. syriacum, a recombina...
Chapter
Medicago truncatula is a model species to study legume defense mechanisms against rust infection. Different studies have characterized its host interactions with Uromyces striatus (alfalfa rust), and its nonhost resistance to other related rust species. The present review assesses how these studies allowed to understand the mechanisms of resistance...
Chapter
Medicago truncatula is an important annual pasture legume used nowadays as a model species to study legume‐specific traits. As with most legume species, M. truncatula can be infected by powdery mildew hampering its yield. The large economic losses caused by powdery mildew in most crops have driven intensive studies to identify resistance and establ...
Chapter
A number of species of flowering plants have adapted to parasitize other plants, some of them posing severe constraints to major crops including grain and forage legumes. The most economically damaging on legumes are the broomrapes (Orobanche and Phelipanche), but also species of Striga, Alectra, and Cuscuta can be also of importance. The level of...
Article
Full-text available
Background: Pea (Pisum sativum) is one of the most important temperate grain legumes in the world, whose production is severely constrained by the pea aphid (Acyrthosiphon pisum). Wild relatives, such as P. fulvum, are valuable sources of allelic diversity to improve the genetic resistance of cultivated pea species against A. pisum attack. To unra...