Diego Ismael Rocha

Diego Ismael Rocha
Universidade Federal de Viçosa (UFV) | UFV · Department of Crop Science

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84
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Publications

Publications (84)
Article
Full-text available
The organogenic potential of root explants derived from cultured seedlings of Bixa orellana L. (annatto) was investigated in response to different incubation conditions and either 4.44 mu M 6-benzyladenine, 4.54 mu M Thidiazuron, or 4.56 mu M Zeatin. Explants cultured in liquid media with agitation generally showed better development of adventitiou...
Article
The aim of this study was to establish a system of in vitro germination and propagation of Guazuma ulmifolia Lam. micro-cuttings (Malvaceae). Seeds were inoculated into test tubes containing different concentrations (half-strength (½) and full-strength) of Murashige and Skoog (MS) basal salts and sucrose (0, 10, 20, 40, and 80 g L −1). For the indu...
Article
Full-text available
The secretion dynamics and chemical compositions of flower nectar can vary according to the plant physiology and the pollinator type. Bat-pollinated flowers are commonly associated with nocturnal nectar secretion as well as large amounts of nectar per flower, which is generally diluted and composed mostly of hexose sugars. The nectar traits in bat-...
Article
Although the Atlantic Forest is considered a humid environment, plants that grow on rock outcrops present adaptive strategies, which correlate with tolerance to desiccation. Colleters are secretory structures that protect meristems and developing organs from dehydration and attack by herbivores or pathogens. Colleters are common in Gentianaceae; ho...
Article
Full-text available
The present study describes a new regeneration system based on somatic embryogenesis from mature endosperm Passiflora cincinnata Mast. cultures. Moreover, the morpho-agronomic and phenological traits, as well as enzymatic activity of regenerated triploid emblings are compared to those of diploids. Mature endosperms were cultured on Murashige and Sk...
Article
Full-text available
Morphometry of fruits and seeds is a useful and important information for phenotyping and genetic characterization of crop plant species and, for conservation of endangered species, as is the case of Butia purpurascens Glassman. In this study, we determined comparatively the morphometric features of fruits and pyrenes between yellow and magenta B....
Article
In assessing the quality of seed lots, the vigor tests are complementary to the germination test and they identify differences in the degree of deterioration of the lots. For safflower, there is little information regarding these tests. In this way, the intention of this study was to adapt the accelerated aging test methodology to assess the physio...
Article
Full-text available
Colleters are secretory structures involved in the protection of young and developing plant organs. Although the presence of colleters in Gentianales is described as a synapomorphy, studies on the morphofunctionality of colleters and the mechanisms underlying the synthesis and release of colleter secretion in Gentianaceae are scarce. Here, we descr...
Article
Full-text available
Colleters produce a secretion composed of hydrophilic and/or lipophilic substances which lubricates and protects the shoot apical meristem against biotic and abiotic agents. Little attention has been given to these structures in Clusiaceae. In the present study, the structure and development of the leaf colleters of Clusia criuva subsp. criuva were...
Article
Si is an essential nutrient for diatom growth. It is involved in frustule formation and is directly linked to the cell cycle. Despite numerous studies analyzing Si starvation in diatoms, the interaction between Si and central metabolism remains poorly understood. Accordingly, we analyzed the metabolite profiles of two freshwater strains of Nitzschi...
Article
Seminiferous propagation of Dietes bicolor is hindered by the probable physical and/or morphophysiological dormancy. The objective was to analyze the efficacy of different methods of overcoming dormancy in D. bicolor seeds to determine a possible pre-germination treatment for the species. Two experiments were conducted: (I) Evaluation of the breaki...
Article
Full-text available
The cellular and biochemical features associated with expression of pluripotency in endosperm cells during de novo shoot organogenesis, as well as the cytogenetic and morpho-agronomic features of regenerated Passiflora foetida triploid plants has been recently reported. Endosperms were cultured on Murashige and Skoog medium supplemented with 0.5, 0...
Article
Conventional in vitro propagation may affect normal morphophysiological characteristics and survival during the acclimatization of commercial plants, such as Etlingera Elatior (Jack) R.M. Smith. CO2 enrichment, gas-permeable membranes, and reduced carbohydrate concentration in culture media can be used to mitigate these effects. Here, we aimed to d...
Article
Full-text available
Pfaffia glomerata, popularly known as Brazilian-ginseng, stands out as a species of medicinal interest that has a high photoautotrophic potential for in vitro cultivation. This study aimed to analyze cell wall components of P. glomerata during in vitro cultivation in a CO2-enriched atmosphere. For this, P. glomerata plants were grown in MS medium w...
Article
Plant regeneration from reproductive organs is a poorly understood route in passion fruit. The present study provides a morpho-hystological description of somatic embryogenesis from anther culture in Passiflora gibertii N. E. Br., as well as a phytochemical profile and genetic analysis of ploidy in the generated embryos. Flower buds were collected...
Article
Clusiaceae species are known globally for the economic importance of their secretory structures; however, reports of colleters in the family are scarce. In the present study, we characterised the leaf colleters in Clusia burchellii by investigating the morphology and anatomy of the glands, the histolocation of the major chemical substances present...
Article
Induction of somatic embryogenesis from in vitro-cultivated anthers represents a recent but poorly understood regeneration pathway for passion fruit species. Here, we aimed to develop an efficient system to produce and proliferate somatic embryos from cultivated anthers of Passiflora cincinnata. The floral buds were categorized into five different...
Article
We aimed to evaluate the organogenic responses of root explants of Psidium schenckianum and P. guineense inoculated onto JADS liquid culture medium containing different concentrations (0.00, 2.22, and 4.44 µM) of 6-benzyladenine (BA) alone or in combination with 0.054 µM α-naphthaleneacetic acid (NAA). After 60 days of culture, the percentage of ex...
Article
Full-text available
Tissue culture enables the efficient clonal propagation of plants. This prevents the loss of genetic information in the natural environment and, in the case of medicinal plants, helps produce bioactive metabolites. Here, we used Vernonia condensata to understand how in vitro culture conditions impacted development, anatomy, physiological performanc...
Article
Full-text available
• Species of the genus Thismia Griff. are small herbs considered mycoheterotrophic, due to an intimate relationship with fungi. They are achlorophyllous, with complex floral structure andlittle information on reproductive strategies.. This study evaluated structural and ecological aspects associated with the dispersal of seeds of Thismia panamensis...
Article
Plant in vitro growth performance is influenced by environmental, physical, and chemical conditions. The present study evaluated the mutual effect of light, gelling agents, and sucrose concentration on leaf anatomical structure of in vitro-grown Polygala paniculata. Nodal segments were inoculated on agar- or Phytagel®-gelified medium supplemented w...
Article
Full-text available
The gabirobeira is a species native to the Brazilian Cerrado with potential for use in cropping systems. This study evaluated the effect of the cytokinin 6-benzylaminopurine (BAP) on root cuttings of gabirobeira (Campomanesia adamantium). The plant material was obtained from gabirobeira progenies of one and two-years-old. The cuttings were segmente...
Article
Passiflora cincinnata stands out among Passifloraceae because of its medicinal properties and its resistance to pathogens, which promotes its use as a rootstock for other Passiflora species. A valuable strategy for obtaining disease-resistant passion fruit plants is represented by genetic transformation; however, this requires efficient regeneratio...
Article
Full-text available
The availability of reliable and reproducible micropropagation systems is a decisive step to successfully propagate plant species with slow growth and low germination rate. The effect was investigated of gibberellin (GA) and cytokinin (CK) in the in vitro germination and development of Dietes bicolor (Steud.) Sweet ex Klatt. Seeds were inoculated i...
Article
Full-text available
Triploid plants represent an important resource for the breeding of fruit and ornamental plants. Here, we report a facile and robust system for regenerating passion fruit triploid plants (Passiflora cincinnata Mast.) through in vitro endosperm culture. We describe the histological and biochemical aspects associated with the de novo shoot organogene...
Article
Full-text available
The propagation of ‘cajá-manga’ (Spondias dulcis) is usually performed by seeds. The presence of dormancy is an obstacle for seedling production and the commercial use of its main product, the fruit. This research aimed to evaluate the use of phytoregulators associated with the scarification of the distal region of the embryo in dormancy overcoming...
Article
• The partial or complete loss of chlorophylls, or albinism, is a rare phenomenon in plants. In the present study, we first report the occurrence of albino Delonix regia seedlings and describe the morpho‐physiological changes associated with albinism. • Wild‐type and albino seedlings were characterized. Leaflets samples were processed following usu...
Article
The adjustment of protocols for the in vitro culture is fundamental for the conservation and application of biotechnological techniques of Psidium spp. This study has been aimed to determine the most appropriate culture medium formulation for the in vitro culture of Psidium guajava ‘Paluma’ (cv. GP) and a wild relative species, P. guineense. Seedli...
Article
Full-text available
An iron ore pelletizing plant in southeastern Brazil exposes the tropical coastal ecosystems known as restinga to emissions of dust, iron solid particulate matter, and sulfur dioxide (SO2). We aimed to assess the effects of those emissions on the leaf morphology and anatomy of the restinga species Byrsonima sericea, Cordia verbenacea, and Psidium g...
Article
Full-text available
Key message Cytokinin supplementation was essential for the induction of shoot proliferation and in vitro organogenesis from shoot apices and cotyledons of D. regia, respectively. Abstract Micropropagation techniques have been established for woody species to facilitate their mass clonal propagation and to better understand their processes of rege...
Article
This study aimed to propose a somatic embryogenesis protocol for lisianthus (Eustoma grandiflorum) using leaf explants, as well as to characterize the cytological alterations involved in the regeneration process and ploidy fidelity of regenerated plants. The explants showed responsiveness and induced formation of pro-embryogenic structures in mediu...
Chapter
This book covers the biotechnology of all the major perennial fruit and nut species, including Actinidia spp., Anacardium occidentale , Mangifera indica , Pistacia vera , Annona spp., Cocos nucifera , Elaeis guineensis , Phoenix dactylifera , Ananas comosus , Carica papaya , Garcinia mangostana , Diospyros kaki , Vaccinium spp., Castanea spp., Cary...
Article
Full-text available
Environmental conditions of grow can modify leaf structure and metabolite production. Neem plants produce a high amount of medicinal metabolites and contain biopesticide terpenoids with low toxicity. However, the high genetic variation and the low material quality, besides the environmental modifications warn to the need of biotechnological techniq...
Article
Full-text available
The combination of different colors from light-emitting diodes (LEDs) may influence growth and production of secondary metabolites in plants. In the present study, the effect of light quality on morphophysiology and content of 20-hydroxyecdysone (20E), a phytoecdysteroid, was evaluated in accessions of an endangered medicinal species, Pfaffia glome...
Article
Full-text available
P. grandiflora is a known ornamental plant with abundant flowering. The flowers exhibit varied coloration with distinct forms and simple folded petals and/or multiple. The objective of this work was to induce regeneration via organogenesis and in vitro flowering of P. grandiflora. Nodal segments of seedlings germinated in vitro were used as explant...
Article
Full-text available
In vitro photoautotrophic propagation system has been successfully established for Pfaffia glomerata, a medicinal species that produces the phytoecdysteroid 20-hydroxyecdysone (20E) under forced ventilation and CO2 enrichment. For that, an adequate supporting material with high porosity in place of agar is required. In this study, we investigated m...
Article
Full-text available
Understanding the in vitro performance of royal poinciana explants cultured in media supplemented with different types and concentrations of cytokinins may aid in the optimization of regeneration systems established for this woody species. In the present study we evaluate the in vitro performance of royal poinciana cotyledonary explants cultured in...
Article
Por apresentar qualidade organoléptica, a ciriguela tornou-se bastante apreciada, despertando interesse para seu cultivo comercial. Todavia, ainda são incipientes os estudos com essa fruteira. Dessa forma, objetivou-se com este trabalho caracterizar frutos de ciriguela em três estádios de amadurecimento em Jataí-GO. Os frutos foram coletados de pla...
Article
A obtenção de mudas de qualidade é resultante da utilização de substratos uniformes e estáveis, que garantem a eficiência do crescimento e desenvolvimento das plantas. No presente estudo, foi avaliada a propagação do maracujazeiro-azedo (Passiflora edulis f. flavicarpa) e da cultivar FB 200 (Flora Brasil). O experimento foi conduzido no viveiro da...
Article
Full-text available
The ornamental flower sector has growing over the past years worldwide with potential for further expansion. Among the ornamental plants, Passiflora species have been gaining ground in the market, mainly in European and North American countries. However, the market aiming the use of these species in ornamentation is still poorly explored. The inclu...
Article
Full-text available
The propagation of gabirobeira is carried out by seeds. However, the recalcitrance and not tolerance of the seeds to the storage compromise the formation of seedlings. Thus, the propagation by cuttings can be an option, being able to provide significant results in different seasons. The objective of this work was to evaluate the vegetative propagat...
Article
Full-text available
The present study reports a shoot organogenesis-based system for in vitro regeneration of Passiflora miniata, an Amazonia passion fruit species. Root segments were cultured in Murashige and Skoog (MS) medium supplemented with different concentrations (range 2-9 µM) of 6-benzyladenine (BA); thidiazuron (TDZ) or kinetin (KIN). Plant growth regulators...
Article
Full-text available
The combination of different plant growth regulators can result in beneficial effects in the induction of in vitro morphogenetic pathways. The present study reports the effect of 24-epibrassinolid (24-epiBR; brassinosteroid) when added alone and in association with N⁶-(2-isopentnyl) adenine (2-iP; cytokinin) in the induction of direct somatic embry...
Chapter
In vitro plant regeneration systems have turned into invaluable tools to plant biotechnology. Despite being poorly understood, the molecular mechanisms underlying the control of both morphogenetic pathways, de novo organogenesis and somatic embryogenesis, have been supported by recent findings involving proteome-, metabolome-, and transcriptome-bas...
Data
Figure S2. Construction, expression and leaf phenotype of ATML1 pro :TCP5‐GFP.
Data
Figure S3. Floral phenotypes in TCP5 overexpression and mutant lines.
Data
Table S3. List of Gene Ontology terms (full analysis).
Data
Figure S1. Expression pattern of TCP5 in Arabidopsis petals during early and later stages of flower development.
Data
Figure S7. Phenotypes of the 35S pro :TCP5‐GR mutant after dexamethasone treatment.
Data
Figure S8. Phenotypical alterations in petals of mutants deficient in ethylene reception and signal transduction.
Data
Table S1. Lists of differentially expressed genes after RNA‐seq analysis of all samples used in this study.
Data
Figure S4. Graphic representation accompanying the cell morphology phenotyping.
Chapter
The process of somatic embryogenesis has become an essential asset, as it enables plant regeneration and large-scale propagation. Our research team has pioneered a reproducible protocol for somatic embryogenesis using mature zygotic embryo of P. cincinnata. Here, we describe in details the protocol for P. cincinnata, in which the explants were expo...
Article
Full-text available
Due to the triploid nature of endosperm, an embryonic reserve tissue, in vitro culture of endosperm tissues has been considered a direct method for production of polyploids. In the present study, we report the establishment of an in vitro regeneration system from endosperm culture for production of triploid Passiflora edulis plants, the main commer...
Article
Flowers of most dicotyledons have petals that, together with the sepals, initially protect the reproductive organs. Later during development petals are required to open the flower and to attract pollinators. These diverse set of functions demand a tight temporal and spatial regulation of petal development. We studied the functioning of the Arabidop...
Article
Full-text available
The aim of the present study was to establish a regeneration system via de novo organogenesis from different types of non-meristematic explants of Passiflora cristalina. Leaf, hypocotyl, root segments, cotyledons, and endosperm of P. cristalina seeds were inoculated in Murashige and Skoog (MS)-basal medium, supplemented with different concentration...
Article
Full-text available
The wild grass species Brachypodium distachyon(L.) has been proposed as a new model for temperate grasses. Among the biotechnological tools already developed for the species, an efficient induction protocol of somatic embryogenesis (SE) using immature zygotic embryos has provided the basis for genetic transformation studies. However, a systematic w...
Article
Full-text available
This study demonstrated that somatic embryogenesis and de novo shoot organogenesis-based systems of root-derived Lisianthus (Eustoma grandiflorum) explants can be alternatively induced by exogenous supply of auxin or cytokinin. Somatic embryogenesis was observed when root explants were cultured in the dark on Murashige and Skoog-based medium supple...
Article
Nitrogen deficiency can increase the lipid content in certain microalgae species, including diatoms. However, the molecular and metabolic basis of such changes remains rather unclear. We analyzed strains of freshwater _Nitzschia palea_ collected from a eutrophic pond and from an artificial rock. The habitats, differing in light and nutrient availab...