Diego Morales

Diego Morales
Universitat Rovira i Virgili | URV · Department of Biochemistry and Biotechnology

PhD

About

30
Publications
4,297
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541
Citations
Citations since 2017
28 Research Items
541 Citations
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2017201820192020202120222023050100150
2017201820192020202120222023050100150

Publications

Publications (30)
Article
Full-text available
Shiitake mushrooms (Lentinula edodes) contain compounds with hypocholesterolemic and immune-modulatory activities such as ergosterol and chitin that can be partially transformed into vitamin D and chitosan to yield extracts with enhanced bioactivities. A method was optimized to increase vitamin D2 levels by irradiating a non-soluble fraction obtain...
Article
Full-text available
Hypertension (HTN) is the leading cause of premature deaths worldwide and the main preventable risk factor for cardiovascular diseases. Therefore, there is a current need for new therapeutics to manage this condition. In this regard, protein hydrolysates containing antihypertensive bioactive peptides are of increasing interest. Thus, agri-food indu...
Article
Truffles are highly valued by their aromatic properties and can aromatize food products. However, the truffle aroma could be reduced or lost with heat treatments (pasteurization and sterilization) necessary for products security and safety. In this study, sunflower oil and honey were aromatized with black truffle (lyophilized and fresh) using two d...
Article
Full-text available
Legumes have been widely consumed and used to isolate bioactive compounds, mainly proteins. The aim of this study was to review the beneficial actions of different legumes proteins and peptides updating the main findings that correlate legumes consumption and the effects on non-transmissible chronic diseases, specifically metabolic syndrome. An exh...
Article
Full-text available
Arbutus unedo, commonly named ‘strawberry tree’ (ST), is a Mediterranean native plant that represents a relevant source of biologically active fractions and compounds. ST fruits, traditionally used with culinary and medicinal purposes, along with other components (leaves, roots, honeys, etc.), have been subjected to varied extraction procedures to...
Preprint
Full-text available
Shiitake mushrooms ( Lentinula edodes ) contain compounds with hypocholesterolemic and immune-modulatory activities such as ergosterol and chitin that can be partially transformed into vitamin D and chitosan to yield extracts with enhanced bioactivities. A method was optimized to increase vitamin D 2 levels by irradiating a non-soluble fraction obt...
Article
Full-text available
Hypertension (HTN) is one of the main cardiovascular risk factors and is considered a major public health problem. Numerous approaches have been developed to lower blood pressure (BP) in hypertensive patients, most of them involving pharmacological treatments. Within this context, natural bioactive compounds have emerged as a promising alternative...
Article
Full-text available
PurposeThe aim of this study was to evaluate the hypocholesterolemic, immune- and microbiota-modulatory effect of a mushroom extract in hypercholesterolemic subjects.MethodsA randomized, controlled, double-blind, and parallel clinical trial was carried out with subjects from 18 to 65 years old (n = 52) with untreated mild hypercholesterolemia. Volu...
Article
Truffles are a well-known worldwide product mainly appreciated by their unique aroma, which is composed by more than 50 volatile compounds. However, to this day, no one has accomplished to find the aromatic key that evokes the real aroma of truffles for its use as food flavoring. Among them, black truffle was selected for extraction with supercriti...
Article
Background Oak trees (Quercus spp.) constitute a relevant source of fractions and ingredients that have been tested regarding their biological activities. The high number of species, varieties and materials that can be found through this genus has led to a great diversity of extracts that can be investigated regarding their potential to improve hum...
Article
[Background] The growing consumption of kombucha beverages in the last decade has motivated an increase in the number of scientific studies investigating their biological activities. Their specific microbiological and chemical composition led to the generation of interesting bioactive fractions. [Scope and approach] Although many in vitro analyses...
Article
The effects of gamma irradiation (0.5, 1.0, 1.5 and 2.5 kGy doses) on Tuber aestivum packaged under modified atmosphere was evaluated. The respiration rate, microbial populations, sensory characteristics and content of bioactive compounds (total carbohydrates, chitins, β-glucans, proteins, total phenols and sterols) were monitored from immediately...
Article
Full-text available
The interest in the research about underexploited foods has increased in the last two decades. Pseudocereals have been consumed by the ancient populations for hundreds of years. These plants that do not belong to the family of cereals, but that have properties and uses similar to them, stand out among underexploited foods. Some of the most represen...
Article
Truffles, besides the appreciated aromatic compounds, contain other molecules with interesting bioactive properties. A screening of fungal sterols and β-glucans within different truffle species and locations was carried out. These compounds were extracted with pressurized liquids (PLE) generating enriched fractions. Extraction efficiency was studie...
Article
A supercritical extraction plant (with a 6 L extraction cell) was successfully used to obtain ergosterol-enriched extracts from Lentinula edodes (40 ºC, 225 bar, 1-5h, 20 L/h recirculated CO2). Ergosterol (ERG) and the SFE extract (SFE) with the highest ergosterol concentration were also microemulsified and submitted to in vitro digestion to study...
Article
A polysaccharide-enriched extract obtained from Lentinula edodes was submitted to several purification steps to separate three different D-glucans with β-(1→6), β-(1→3),(1→6) and α-(1→3) linkages, being characterized through GC-MS, FT-IR, NMR, SEC and colorimetric/fluorimetric determinations. Moreover, in vitro hypocholesterolemic, antitumoral, ant...
Article
The effects of electron-beam or gamma irradiation (both applied at 1.5 kGy and 2.5 kGy) and subsequent storage under modified atmospheres (35 d at 4 °C) were evaluated on total carbohydrates, chitins, β-glucans, proteins, total phenols, sterols and ergocalciferol concentrations of Tuber melanosporum ascocarps. Irradiation procedures reduced chitin...
Article
Innovative technologies as ultrasound-assisted extraction (UAE) (550 W, 60% amplitude, 50 °C) or subcritical water extraction (SWE) (200 °C, 11.7 MPa) were more effective than hot water extractions to obtain β-glucan-enriched fractions from shiitake mushrooms. UAE required longer extraction time (60 min) than SWE (15 min). Combination of UAE + SWE...
Article
The parameters to extract polysaccharide-enriched fractions (PEF) from mushrooms using MAE (microwave-assisted extraction) were adjusted following a full factorial 3 ² experimental design. The highest yield and total carbohydrate values, using Lentinula edodes as model mushroom, were obtained at 180 °C and 30 min. Several mushroom species were subm...
Article
Eritadenine is a hypocholesterolemic compound that was found in several mushroom species such as Lentinula edodes, Marasmius oreades, Amanita caesarea (1.4, 0.7 and 0.6 mg/g dw, respectively). It was synthesized during all developmental stages being present in higher concentrations in the skin of shiitake fruiting bodies. When submitted to traditio...
Chapter
Edible mushrooms are an interesting source of valuable compounds including sterols, fatty acids, carbohydrates, dietary fibers, vitamins, proteins, phenolic compounds, minerals and specific bioactive molecules. Many of those compounds show health beneficial properties such as antioxidant, antitumor, immunomodulatory, hypocholesterolemic, hypotensiv...
Article
A pilot-scale process combining extraction of Shiitake mushroom (Lentinula edodes) powder in water (98 °C, 1 h), cross-flow microfiltration and reverse osmosis (nanofiltration) was performed to obtain β-glucan-rich extracts. Suspensions (45–80 L) obtained after 3 extractions were clarified by microfiltration reducing their turbidity to <1 NTU. Memb...
Article
Three extraction methods were sequentially combined to obtain fractions from Lentinula edodes (shiitake mushrooms) containing bioactive compounds against cardiovascular diseases (CVDs). Fruiting bodies were first extracted with plain water, obtained residue was then submitted to supercritical fluid extraction (SFE) and remaining residue submitted t...
Article
Cholesterol levels are strictly regulated to maintain its homeostasis therefore, if it is not absorbed with the diet, the cholesterol biosynthetic pathway is enhanced and vice versa. Nowadays, the commonly prescribed therapeutic treatments for hypocholesterolemic patients are targeted toward the reduction of both cholesterol intestinal absorption a...
Article
The combination of supercritical fluid extraction followed by UV-irradiation resulted in an interesting strategy to obtain ergosterol- and Vitamin D2-enriched extracts from Lentinula edodes to design novel functional food. Extractions carried out following a specific experimental design pointed out that extraction yields and ergosterol concentratio...
Article
Water extracts from Pleurotus ostreatus containing no statins showed HMGCR (3-hydroxy-3-methyl-glutaryl CoA reductase) inhibitory activity (in vitro) that might be due to specific water-soluble polysaccharides (WSP) because when isolated and deproteinized, increasing concentrations of the WSP extract induced higher inhibition. The WSP extract conta...
Conference Paper
Full-text available
Eritadenine (2(R),3(R)-dihydroxy-4-(9-adenyl)butanoic acid) is an alkaloid derived from the secondary metabolism of edible mushrooms. It was initially isolated from shiitake mushrooms (Lentinus edodes) although, later on was also found in the common button mushroom (Agaricus bisporus). This compound showed hypocholesterolemic and hypotensive proper...

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Projects

Project (1)
Project
The objectives of this project are: - Design of extraction systems (individual and sequential) using green technologies (SFE, PLE, MWE...) to obtain large quantities of bioactive extracts from mushrooms. - Adjustment of the formulation of functional foods by mixing commercialized foods and obtained extracts, evaluating their bioaccesibility, bioavailability and biosafety. - Validation through clinical trials: clinical, dietetic and biochemical parameters, effect on enteric microbiota, etc.