Diego Lijavetzky

• PhD
• Principal Researcher at National Scientific and Technical Research Council

Zanetti et al use convincing biophysical and cellular assays to investigate the interaction of the birnavirus VP3 protein with the early endosome lipid PI3P. The study provides valuable insights and will be of interest to virologists. In future studies, it would be interesting to demonstrate that VP3-PIP3P is a specific interaction and not a genera...

Zanetti et al use convincing biophysical and cellular assays to investigate the interaction of the birnavirus VP3 protein with the early endosome lipid PI3P. The study provides valuable insights and will be of interest to virologists. In future studies, it would be interesting to demonstrate that VP3-PIP3P is a specific interaction and not a genera...

Birnaviruses are a group of double-stranded RNA (dsRNA) viruses infecting birds, fish and insects. Early endosomes (EE) constitute the platform for viral replication. Here, we study the mechanism of birnaviral targeting of EE membranes. Using the Infectious Bursal Disease Virus (IBDV) as a model, we validate that the viral protein 3 (VP3) binds to...

Plants go through two distinct stages in their vegetative phase, with the juvenile stage being characterized by a lack of maturity to respond to flowering induction stimuli and the adult stage marked by the presence of this capacity. Phase transition has been extensively analysed in herbaceous species such as Arabidopsis and maize, where the sequen...

To preserve their varietal attributes, established grapevine cultivars (Vitis vinifera L. ssp. vinifera) must be clonally propagated, due to their highly heterozygous genomes. Malbec is a France-originated cultivar appreciated for producing high-quality wines and is the offspring of cultivars Prunelard and Magdeleine Noire des Charentes. Here, we h...

Birnaviruses form a distinct class of double-stranded RNA (dsRNA) viruses characterized by the absence of a transcription-competent inner core particle. The early endosomes (EE) of cells infected with the infectious bursal disease virus (IBDV) - a prototypical birnavirus and an important avian pathogen - constitute a platform for viral replication....

Birnaviruses form a distinct class of double-stranded RNA (dsRNA) viruses characterized by the absence of a transcription-competent inner core particle. The early endosomes (EE) of cells infected with the infectious bursal disease virus (IBDV) - a prototypical birnavirus and an important avian pathogen - constitute a platform for viral replication....

Birnaviruses form a distinct class of double-stranded RNA (dsRNA) viruses characterized by the absence of a transcription-competent inner core particle. The early endosomes (EE) of cells infected with the infectious bursal disease virus (IBDV) - a prototypical birnavirus and an important avian pathogen - constitute a platform for viral replication....

The first hermaphrodite flowers appeared in grapevine (Vitis vinifera L.) as a consequence of the domestication process. This is a main characteristic of cultivated grapevines differing from their wild dioecious ancestors. Due to the predominant presence of masculinized flowers, accession Criolla Chica Nº2 (CCN2) is catalogued as a flower mutation...

Against a backdrop of climate change and loss of biodiversity, preserving traditional grape varieties and their genetic diversity remains a major challenge for the future. The Catena Institute, in association with a number of Argentinian researchers, has embarked on a project of this kind, focusing on a grape variety that originally came from south...

Grapevine cultivars (Vitis vinifera L. ssp. vinifera) must be clonally propagated because of their highly heterozygous genomes. Malbec, a France-originated cultivar appreciated for the production of high-quality wines, is the offspring of cultivars Prunelard and Magdeleine Noire des Charentes. Here, we have built a diploid genome assembly of Malbec...

Problematic fermentations frequently drive economic losses and logistic problems in the winemaking industry. Previous studies have determined thermal conditions leading to problematic fermentations, selecting two contrasting yeast strains for further transcriptomic analysis. Saccha-romyces cerevisiae SBB11 showed strong thermosensitivity towards he...

Grapevine, as other woody perennials, has been considered a recalcitrant crop to produce transgenic plants. Since the production of transgenic and/or edited plants requires the ability to regenerate plants from transformed tissues, this step is often the biggest bottleneck in the process. The objective of this work is to review the state of the art...

Grapevine cultivars are clonally propagated to preserve their varietal attributes. However, genetic variations accumulate due to the occurrence of somatic mutations. This process is anthropically influenced through plant transportation, clonal propagation and selection. Malbec is a cultivar that is well-appreciated for the elaboration of red wine....

Carrot (Daucus carota L.) is one of the most cultivated vegetable in the world and of great importance in the human diet. Its storage organs can accumulate large quantities of anthocyanins, metabolites that confer the purple pigmentation to carrot tissues and whose biosynthesis is well characterized. Long non-coding RNAs (lncRNAs) play critical rol...

Carrot ( Daucus carota L.) is one of the most cultivated vegetable in the world and of great importance in the human diet. Its storage organs can accumulate large quantities of anthocyanins, metabolites that confer the purple pigmentation to carrot tissues and whose biosynthesis is well characterized. Long non-coding RNAs (lncRNAs) play critical ro...

Grapevine ( Vitis vinifera L.) cultivars are clonally propagated to preserve their varietal attributes. However, novel genetic variation still accumulates due to somatic mutations. Aiming to study the potential impact of clonal propagation history on grapevines intra-cultivar genetic diversity, we have focused on ‘Malbec’. This cultivar is apprecia...

Grapevine phenology is being modified by climate change, particularly by the increase of temperatures that affect grape attributes for wine production. Besides the existing oenological and viticultural approaches, the thorough exploration of the current intra-cultivar genetic variability to select late-ripening genotypes emerges as an interesting a...

In grapevine, proanthocyanins (PAs) and anthocyanins are obtained via the phenylpropanoid pathway. These metabolites are synthesized in specific tissues of the berry and accumulate in different developmental stages. The transcriptional control of the route is affected by transcription factors (TFs), mainly R2R3-Myb type, which can act as activators...

The analyses of the grapevine (Vitis vinifera L.) genome have revealed an unusually large and closely related stilbene synthase (VvSTS) gene family. Interestingly, despite the high sequence similarity among those genes, several studies have observed clear differences between their expression patterns. Here, we studied the transcriptional responses...

Grape color somatic variants that can be used to develop new grapevine cultivars occasionally appear associated to deletion events of uncertain origin. To understand the mutational mechanisms generating somatic structural variation in grapevine, we compared the Tempranillo Blanco (TB) white berry somatic variant to its black berry ancestor, Tempran...

The aim of this chapter is to provide a description of the latest scientific advances in the field of gene functional analysis in grapevine. It provides general information about the studies conducted during the past decade to understand the natural variation of this plant and how this information has been exploited for the understanding of traits...

Fruit size is a highly important trait for most fruit and vegetable crops. This trait has been a main selection target and could be involved in divergent selection processes leading to the differentiation between modern table and wine cultivars. Even though its determination is highly influenced by cultural practices, several regions within the gra...

Understanding the relationship between genotype and phenotypic diversity is one of the most important challenges in plant biology. Thus, is essential to have the knowledge and comprehension about the different quality traits, as well as the nature and function of the genes controlling such traits. Malbec, the emblematic variety of Argentine viticul...

Grapevine leafroll is one of the most important viral disease affecting grapevines the world over. One of the viruses associated with this disease is Grapevine leafroll associated virus 2 (GLRaV-2), which has two remarkable properties: high genetic variability (up to date at least 6 different lineages have been reported) and the unique ability amon...

Fruit size is a highly important trait for most fruit and vegetable crops. Several regions within the grapevine genome have been identified influencing berry size, mainly those related to seedlessness, but also particular transcription factors affecting cell number and/or cell size of flowers or berries.In this sense, the control of the intrinsic o...

The high effectiveness of cyclic oligosaccharides like cyclodextrins in the production of trans-resveratrol in Vitis vinifera cell cultures is enhanced in the presence of methyl jasmonate. In order to dissect the basis of the interactions among the elicitation responses triggered by these two compounds, a transcriptional analysis of grapevine cell...

La variación genética somática puede contribuir a la variación fenotípica en plantas perennes. Esto es particularmente importante en especies cultivadas como la vid en la que muchas variedades se han propagado vegetativamente durante siglos. La variación fenotípica entre las líneas y clones que derivan de un zigoto original se deben a mutaciones y...

Berry organoleptic properties are highly influenced by ripening environmental conditions. In this study, we used grapevine fruiting cuttings to follow berry ripening under different controlled conditions of temperature and irradiation intensity. Berries ripened at higher temperatures showed reduced anthocyanin accumulation and hastened ripening lea...

genetic and genomic information available in grapevine in combination with the analyses of archaeological remains can help to shed light on questions regarding its domestication process, such as the number and location of domestication events, the temporal sequence of the domestication process or the identification of some of the genes that drove t...

Background: Ripening of fleshy fruit is a complex developmental process involving the differentiation of tissues with separate functions. During grapevine berry ripening important processes contributing to table and wine grape quality take place, some of them flesh- or skin-specific. In this study, transcriptional profiles throughout flesh and ski...

Probesets showing significant differences between flesh and skin throughout berry ripening. (XLSX)

PCA plot of ‘Muscat Hamburg’ pericarp samples according to their expression data. A, PCA plot of flesh and skin ripening samples according to their RMA expression data. The first (PC1) and the third (PC3) principal components are represented (from a six component PCA after data centering). Expression data from probe sets matching the same transcrip...

Ripening evolution of individual soluble sugars and organic acids. (DOCX)

PCA plot of the unique transcripts according to their expression in flesh and skin throughout ripening. A, PC1 and PC2 are represented. Transcripts most contributing to the distribution in the PC1 (|PC1 loading score| >10) are highlighted. B, Expression profile of transcripts with |PC1 loading score| >10. C, PC1 and PC2 are represented. Transcripts...

Additional physiological data at different stages of berry ripening. (DOCX)

Functional categories enriched in the unique transcripts with higher PCA loading scores. (XLSX)

Probesets showing significant changes throughout ripening and taking place in the same way in flesh and skin. (XLSX)

Unique transcript PCA loading scores, annotation, and functional categorization. (XLSX)

Functional enrichment in clusters of transcripts differentially accumulated between flesh and skin. (XLSX)

Functional enrichment in clusters of transcripts changing in the same way in flesh and skin throughout ripening. (XLSX)

One of the most important agronomic problems in the production of sorghum [Sorghum bicolor (L.) Moench] in humid climates is pre-harvest sprouting (PHS). A molecular linkage map was developed using 112molecular markers in an F2 mapping population derived from a cross between IS 9530 (high resistance to PHS) and Redland B2 (susceptible to PHS). Two...

Supplementary Tables S1 to S5. Table S1: Plant samples analyzed. Table S2: Plant samples used for the stability studies of the 48 SNP set. Table S3: Basic information on the 238 SNP analyzed. Table S4: Genetic maps features. Table S5: Number of progenies with heterozygous markers in at least one progenitor.

Rapid and consistent genotyping is an important requirement for cultivar identification in many crop species. Among them grapevine cultivars have been the subject of multiple studies given the large number of synonyms and homonyms generated during many centuries of vegetative multiplication and exchange. Simple sequence repeat (SSR) markers have be...

Resumen El estudio profundo de las variaciones observadas en clones de vid para distintas características fenotípicas, agronómicas o enológicas puede permitir determinar qué potenciales particulares pueden presentar los distintos clones de una colección o viñedo. Además, esta información puede conducir a la determinación de las bases genéticas de l...

In this work, the effect of different inducing factors on trans-resveratrol extracellular production in Monastrell grapevine suspension cultured cells is evaluated. A detailed analysis provides the optimal concentrations of cyclodextrins, methyljasmonate and UV irradiation dosage, optimal cell density, elicitation time and sucrose content in the cu...

Grapevine (Vitis vinifera L.) is an economically important fruit crop. Quality-determining grape components, such as sugars, acids, flavours, anthocyanins, tannins, etc., are accumulated during the different grape berry development stages. Thus, correlating the proteomic profiles with the biochemical and physiological changes occurring in grape is...

Up-regulated functional classes High UV-B. PDF file showing the full list of differentially expressed genes included in the up-regulated functional categories under high UV-B radiation described in Table 1. Positive and negative symbols represent higher or lower transcript levels under UV-B light compared with the control, respectively.

qRT-PCR primers. PDF file describing the DNA primers used for quantitative real time reverse transcription polymerase chain reaction (qRT-PCR).

Full list of genes differentially expressed under high UV-B radiation. PDF file showing a complete list of the genes differentially expressed in the high UV-B treatment including Probe-set ID, Unique grapevine gene ID, Annotation and fold-change.

Full list of genes differentially expressed under low UV-B radiation. PDFgenes file showing a complete list of the genes differentially expressed in the low UV-B treatment including Probe-set ID, Unique grapevine gene ID, Annotation and fold-change.

qRT-PCR expression validation. PDF file illustrating the comparison of gene expression values reported by the GrapeGen Vitis vinifera Affymetrix GeneChip® and by quantitative real-time RT-PCR (qRT-PCR). The microarray log2 (expression ratio) values (y-axis) are plotted against the log2 (expression ratio) obtained by qRT-PCR (x-axis). Linear regress...

Up-regulated functional classes Low UV-B. PDF file showing the full list of differentially expressed genes included in the up-regulated functional categories under low UV-B radiation described in Table 2. Positive and negative symbols represent higher or lower transcript levels under UV-B light compared with the control, respectively.

Down-regulated functional classes High UV-B. PDF file showing the full list of differentially expressed genes included in the down-regulated functional categories under high UV-B radiation described in Table1. Positive and negative symbols represent higher or lower transcript levels under UV-B light compared with the control, respectively.

MapMan annotation. PDF file describing the MapMan BIN structure and the number of genes included in each BIN and subBIN.

MapMan diagrams of all significantly modulated functional categories not included as main Figures in the text. PDF file displaying the pictorial representation of the differentially expressed genes included in the following classes: (a) "Hormone", (b) "Protein metabolism and modification", (c) "Carbohydrate Metabolism", (d) "Metabolism-enzyme famil...

Down-regulated functional classes Low UV-B. PDF file showing the full list of differentially expressed genes included in the down-regulated functional categories under low UV-B radiation described in Table 2. Positive and negative symbols represent higher or lower transcript levels under UV-B light compared with the control, respectively.

Ultraviolet-B radiation (UV-B, 280-315 nm) is a natural component of sunlight, which has numerous regulatory effects on plant physiology. The nature of the response to UV-B is dependent on fluence rate, dose, duration and wavelength of the UV-B treatment. Some reports have analyzed the changes in gene expression caused by UV-B light on several plan...

En los últimos años, el análisis de ADN para identificación de variedades de vid se ha llevado a cabo de modo rutinario utilizando marcadores denominados microsatélites. Estos marcadores son de una enorme utilidad, pero presentan algunas limitaciones, especialmente en lo que respecta a su comparación entre laboratorios. En la actualidad existe otro...

The publication of the genome sequences of inbred grapevine plant PN40024 and the cultivar Pinot Noir has provided a new generation of molecular tools and has opened the way to functional genomics in grapevine. Establishing gene biological function is now a major challenge requiring the parallel development of molecular and genetic information. New...

Plant cell cultures have been shown as feasible systems for the production of secondary metabolites, being the elicitation with biotic or abiotic stimuli the most efficient strategy to increase the production of those metabolites. Vitaceae phytoalexins constitute a group of molecules belonging to the stilbene family which are derivatives of the tra...

Cell viability of grapevine cell suspension cultures treated with MeJA. Cell viability was evaluated by incubating the cells for 1–2 min in fresh Gamborg medium containing 100 μg ml-1 fluorescein diacetate. Fluorescence was observed with a DMRB Leica microscope using a Leica filter (λexc = 490 nm, λemi = 520 nm). (a) and (b) bright field (40×), (c)...

Chromatographic profile of culture medium (A) and cell extract (B) at 168 h. One volume of culture medium is diluted with two volumes of water and twelve of pure methanol. Fifty mg of freeze-dried cells were extracted overnight in 4 mL methanol at 4°C. The extract was diluted with water to a final concentration of 80% (v/v) methanol. Then 30 μL of...

Analysis of stilbenoids in cells at 168 h. Values (in μmole gDW-1) are given as the mean ± standard deviation of three replicates. For methodological details, see Additional File 2.

MIKC(C)-type MADS box genes encode transcription factors that play crucial roles in plant growth and development. Analysis of the grapevine (Vitis vinifera) genome revealed up to 38 MIKC(C)-type genes. We report here a complete analysis of this gene family regarding their phylogenetic relationships with homologous genes identified in other sequence...

We report the generation and analysis of a total of 77,583 expressed sequence tags (ESTs) from two grapevine (Vitis vinifera L.) cultivars, Cabernet Sauvignon (wine grape) and Muscat Hamburg (table grape) with a focus on EST sequence quality and assembly optimization. The majority of the ESTs were derived from normalized cDNA libraries representing...

DNA fragments sequenced in this work. PDF file containing the sequences ID, primers used for their amplification, amplicon size (bp) and associated UniGene or locus identifier.

Grapevine accessions used in this work. PDF file containing the code, name, main use and repository institution of the grape accessions utilized in the present study.

Regression plot of MAF in genotyped accession vs. MAF in original accessions. PDF file displaying the linear regression between the MAF values for the 80 SNPs observed in the sample of ~300 genotyped accessions with the MAF values observed in the original sample of 11 genotypes use in the re-sequencing strategy.

SNPs genotyped using SNPlex™. PDF file containing the SNP IDs, gene diversity, heterozygosity and MAF of the 80 validated SNPs used for the genotyping analysis, together with the MAF observed in the original re-sequenced accessions.

Global and class specific SNP minor allele frequencies. PDF file comparing the global minor allele frequency versus those observed in wild, table and wine classes for the 80 validated SNPs tested in the genotyping analysis.

Single-nucleotide polymorphisms (SNPs) are the most abundant type of DNA sequence polymorphisms. Their higher availability and stability when compared to simple sequence repeats (SSRs) provide enhanced possibilities for genetic and breeding applications such as cultivar identification, construction of genetic maps, the assessment of genetic diversi...

The genetics and biochemistry of anthocyanins and flavonol biosynthesis and their role in plant organ pigmentation is well established in model species. However, the genetic basis of colour variation is species specific and understanding this variation is very relevant in many fruit and flower crop species. Among grape cultivars, there is a wide ge...

These proceedings contain 5 parts, which include several chapters each, discussing: seed development; seed germination and dormancy; desiccation and other stress tolerance, and conservation; seed ecology; and seed biotechnology.

Rust resistance genes Lr37 , Sr38 , and Yr17 are located within a segment of Triticum ventricosum (Tausch) Cess. chromosome 2NS translocated to the short arm of bread wheat chromosome 2AS. Characterization of this chromosome segment by 13 restriction fragment length polymorphism (RFLP) markers indicated that the 2NS translocation replaced approxima...

Dof proteins are a family of plant-specific transcription factors that contain a particular class of zinc-finger DNA-binding domain. Members of this family have been found to play diverse roles in gene regulation of processes restricted to the plants. The completed genome sequences of rice and Arabidopsis constitute a valuable resource for comparat...

The plant vp1 gene encodes a transcription factor originally identified in maize that participates in the control of the transition from embryogenesis to seed germination. Different lines of evidence suggest that vp1 participates in preharvest sprouting (PHS) resistance in cereals, but the genetic relationship is not yet established. Thus, the aim...

Rust resistance genes Lr37, Sr38, and Yr17 are located within a segment of Triticum ventricosum (Tausch) Cess. chromosome 2NS translocated to the short arm of bread wheat chromosome 2AS. Characterization of this chromosome segment by 13 restriction fragment length polymorphism (RFLP) markers indicated that the 2NS translocation replaced approximate...

Pre-harvest sprouting (PHS) in sorghum is related to the lack of a normal dormancy level during seed development and maturation. Based on previous evidence that seed dormancy in maize is controlled by the vp1 gene, we used a PCR-based approach to isolate two Sorghum bicolor genomic and cDNA clones from two genotypes exhibiting different PHS behavio...

Vp1 is a seed-specific gene involved in the control of dormancy and germination. We here present the complete sequence of the sorghum vp1 promoter/enhancer region highlighting its main features, especially the lack of canonical TATA and CAAT boxes and the presence of elements responsive to abscisic acid and light. The region closest to the start of...

A genomic bacterial artificial chromosome (BAC) library of the A genome of wheat has been constructed. Triticum monococcum accession DV92 was selected for this purpose because it is a cultivated diploid wheat and one of the parental lines used in the construction of a saturated genetic map. Leaves from this accession were used to isolate high-molec...

Endosperm texture, i.e. the hardness or softness of the grain, is an important quality criterion in cereals because it determines many grain end-use properties. Grain softness is the dominant trait and is mainly controlled by the Ha locus on the short arm of chromosome 5D in hexaploid bread wheat. Genes for puroindoline a (Pina-D1), puroindoline b...

Abstract A genomic bacterial artificial chromosome,(BAC) library of the A genome of wheat has been constructed. Triticum monococcum,accession DV92 was selected for this purpose because it is a cultivated diploid wheat and one of the parental lines used in the construction of a saturated genetic map.Leaves from this accession were used to isolate hi...

On or before Aug 25, 2016 this sequence version replaced gi:5802139, gi:5802140, gi:5802141, gi:5802142.

On or before Aug 25, 2016 this sequence version replaced gi:5532506, gi:5532507, gi:5532508, gi:5532509.

The genus Eucalyptus groups forest species widely cultivated in the world because of their high productivity and adaptability to different industrial purposes. Eucalyptus dunnii was introduced into Argentina due to its better adaptation to colder climates (including frost tolerance) and industrial quality. In this work silver stained AFLP were appl...

The adaptability of Triticum aestivum to a large range of environments is partially due to genetic differences in sensitivity to vernalization. The most potent gene reducing the vernalization requirement in hexaploid wheat is Vrn-A1. An orthologous vernalization gene, designated Vrn-A m 1, was mapped in the diploid wheat Triticum monococcum betwe...