Didier Marion

Didier Marion
French National Institute for Agriculture, Food, and Environment (INRAE) | INRAE · Biopolymères, Interactions, Assemblages (BIA)

PhD

About

208
Publications
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Publications

Publications (208)
Article
Full-text available
Tomato ( Solanum lycopersicum ) is an established model for studying plant cuticle because of its thick cuticle covering and embedding the epidermal cells of the fruit. In this study, we screened an EMS mutant collection of the miniature tomato cultivar Micro-Tom for fruit cracking mutants and found a mutant displaying a glossy fruit phenotype. By...
Article
The cuticle is a complex polymer matrix that protects all aerial organs of plants, fulfills multiple roles in plant-environment interactions, and is critical for plant development. These functions are associated with the structural features of cuticles, and the architectural modeling of cuticles during plant development is crucial for understanding...
Article
Full-text available
Agro-industrial residues comprise a rich diversity of plant polymers and bioactive compounds, constituting promising sources for the development of materials, including bioplastics, and food supplements, among other applications. In particular, the polyester cutin is abundant in fruit peel, a plentiful constituent of pomace agro-industrial residues...
Article
Full-text available
Terrestrialization of vascular plants, i.e., Angiosperm, is associated with the development of cuticular barriers that prevent biotic and abiotic stresses and support plant growth and development. To fulfill these multiple functions, cuticles have developed a unique supramolecular and dynamic assembly of molecules and macromolecules. Plant cuticles...
Article
Full-text available
The tomato ( Solanum lycopersicum ) fruit has a thick, astomatous cuticle that has become a model for the study of cuticle formation, structure, and properties in plants. Tomato is also a major horticultural crop and a long-standing model for research in genetics, fruit development, and disease resistance. As a result, a wealth of genetic resources...
Article
By mimicking the cutin natural polyester networks of plant cuticles, we produced hydrophobic elastomers by a sustainable process, i.e., using a catalyst- and solvent-free polycondensation of glycerol and hydroxy fatty acids, two by-products of the agro-food industry. The hydroxy fatty acid fraction was obtained by ethanolic alkaline hydrolysis of c...
Article
Full-text available
Grain hardness is an important quality trait of cereal crops. In wheat, it is mainly determined by the Hardness locus that harbors genes encoding puroindoline A (PINA) and puroindoline B (PINB). Any deletion or mutation of these genes leading to the absence of PINA or to single amino acid changes in PINB leads to hard endosperms. Although it is gen...
Preprint
Full-text available
div>By mimicking the cutin natural polyester networks of plant cuticles, we produced hydrophobic elastomers by a sustainable process, i.e., using a catalyst- and solvent-free polycondensation of glycerol and hydroxy fatty acids, two by-products of the agro-food industry. The hydroxy-fatty acid fraction was obtained by alkaline hydrolysis in ethanol...
Article
Minor compounds of cereal grains such as lipids and cell wall polysaccharides play an important role in their milling properties, their transformation into baked cereal products and their nutritional properties. These effects are mainly due to their interactive properties, such as their ability to absorb large amounts of water for polysaccharides o...
Article
The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides. The nature of the cutin‐embedded polysaccharides (CEP) and their association with cutin polyester are still unresolved. Using tomato fruit as a model, chemi...
Article
Full-text available
The main storage components of the maize endosperm are starch, proteins and lipids. Starch and proteins are heterogeneously deposited, leading to the formation of vitreous and floury regions at the periphery and at the centre of the endosperm. The vitreous/floury mass ratio is a key physical parameter of maize end-uses for the food, feed and non-fo...
Preprint
Full-text available
Grain hardness is an important quality trait of cereal crops. In wheat, it is mainly determined by the locus that harbors genes encoding puroindoline A (PINA) and puroindoline B (PINB). Any deletion or mutation of these genes leading to the absence of PINA or to single amin>o acid changes in PINB leads to hard endosperms. Although it is generally a...
Article
Full-text available
The presence of lipids within starch granules is specific to cereal endosperm starches. These starch lipids are composed of lysophospholipids, especially lysophosphatidylcholine (LysoPC) and free fatty acids that strongly impact the assembly and properties of cereal starches. However, the molecular mechanisms associated with this specific lipid rou...
Conference Paper
Additive Manufacturing (AM) opens new perspectives for biopolymers to obtain functional parts, like biomedical devices, by exploiting their biocompatibility and resorbability. Plasticized materials from zein, a storage protein from maize seed endosperm, display thermomechanical properties that could match with Fused Deposition Modeling (FDM). The o...
Article
Full-text available
Cuticular matrices covering aerial plant organs or delimiting compartments in these organs are composed of an insoluble hydrophobic polymer of high molecular mass, i.e., cutin, that encompass some cell wall polysaccharides and is filled by waxes. Cutin is a polyester of hydroxy and-or epoxy fatty acids including a low amount of glycerol. Screening...
Article
Full-text available
Zeins, storage proteins from maize, are suitable for making biobased thermoplastic materials. The rheological behavior of a commercial zein plasticized with 20 w% glycerol was studied in the molten state by steady-state flow experiments in extrusion conditions and oscillatory rheometry. For low residence times, a shear-thinning viscoelastic behavio...
Conference Paper
Additive Manufacturing (AM) of tailored natural biopolymer-based objects by Fused Deposition Modeling (FDM) opens new perspectives for applications such as biomedical temporary devices, or pharmaceutical tablets. This exploits the biocompatibility, resorbability and edibility properties of biopolymers. When adequately plasticized, zeins, storage pr...
Article
Full-text available
Major nutritional and agronomical issues relating to maize (Zea mays) grains depend on the vitreousness/hardness of its endosperm. To identify the corresponding molecular and cellular mechanisms, most studies have been conducted on opaque/floury mutants, and recently on Quality Protein Maize, a reversion of an opaque2 mutation by modifier genes. Th...
Book
Additive Manufacturing (AM) opens new perspectives for biopolymers to obtain functional parts, like biomedical devices, by exploiting their biocompatibility and resorbability. Plasticized materials from zein, a storage protein from maize seed endosperm, display thermomechanical properties that could match with Fused Deposition Modeling (FDM). The o...
Article
Relationships between kernel vitreousness and proteins and starch partitioning to the floury and vitreous regions of the endosperm were monitored in a set of 13 maize inbred lines. Decrease of protein contents from the vitreous to the floury endosperms were mainly assigned to α-zeins. Using Raman microspectroscopy, we observed a protein gradient fr...
Article
Cuticle function is closely related to the structure of the cutin polymer. However, the structure and formation of this hydrophobic polyester of glycerol and hydroxy/epoxy fatty acids has not been fully resolved. An apoplastic GDSL-lipase kown as cutin synthase 1 (CUS1) is required for cutin deposition in tomato fruit exocarp. In vitro, CUS1 cataly...
Article
Content and composition of maize endosperm lipids and their partition in the floury and vitreous regions were determined for a set of inbred lines. Neutral lipids, i.e. triglycerides and free fatty acids, accounted for more than 80% of endosperm lipids and are almost twice higher in the floury than in the vitreous regions The composition of endospe...
Article
The cutin polymers of different fruit cuticles (tomato, apple, nectarine) were examined using MALDI mass spectrometry imaging (MALDI MSI) after an in situ release of the lipid monomers by alkaline hydrolysis. The mass spectra were acquired from each coordinate with a lateral spatial resolution of approximately 100 μm. Specific monomers were release...
Article
Free radicals and oxidative stress are important factors in the biology of aging and responsible for the development of age-related diseases. One way to reduce the formation of free radicals is to boost the antioxidative system by nutrition. Heat treatment of food promote the Maillard reaction which is responsible for their characteristic color and...
Technical Report
Full-text available
Ce panorama présente de façon synthétique les principales orientations des recherches conduites à l’INRA sur les céréales ou qui s'y rapportent en matière de systèmes de production ou d’alimentation. Il couvre la période 2011-2013 et aborde successivement les grands secteurs de recherche : génétique et amélioration des céréales, itinéraires techniq...
Data
This resource is a collection of images of bread slices which can be used for testing algorithms of image texture analysis. The tested breads has been preprared in different technological conditions, and it is expected to discriminate them through image analysis. This data set has currently been used considering the shapes of alveoli of crumbs as t...
Article
Rapport rédigé par une douzaine de membres du GROUPE FILIERE CEREALES de l'INRA, dont l'animateur est Gilles Charmet. Le public visé ne se réduit pas à une catégorie dans celles proposées par PRODINRA, il comprend aussi le public scientifique et le professionnel. Le rapport lui-même et l'annexe 1 sont à diffusion publique, mais l'annexe 2 est confi...
Article
The cutin polymers of different fruit cuticles (tomato, apple, nectarine) were examined using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) after in situ release of the lipid monomers by alkaline hydrolysis. The mass spectra were acquired from each coordinate with a lateral spatial resolution of approximately 100...
Article
Full-text available
Cuticle is a protective layer synthesized by epidermal cells of the plants and consisting of cutin covered and filled by waxes. In tomato fruit, the thick cuticle embedding epidermal cells has crucial roles in the control of pathogens, water loss, cracking, postharvest shelf-life and brightness. To identify tomato mutants with modified cuticle comp...
Article
Full-text available
Fruit peels are waste by-products generated by industrial tomato processing. The cuticular fraction of tomato peels is composed of a polyester, rich in hydroxy-fatty acids. After depolymerization, these fatty acids are isolated and easily dispersed in water into unilayer vesicles by using lysine and choline counter-ions. These vesicles form highly...
Article
Proteomics has emerged recently as a powerful analytical method to investigate proteins. Its scope of use has considerably enlarged in the past years including in the fi eld of feed and food sciences. Through a series of studies conducted at I.N.R.A., we illustrate the interest of proteomic approaches to improve plant breeding, technological proces...
Article
Full-text available
The plant cuticle consists of cutin, a polyester of glycerol, hydroxyl, and epoxy fatty acids, covered and filled by waxes. While the biosynthesis of cutin building blocks is well documented, the mechanisms underlining their extracellular deposition remain unknown. Among the proteins extracted from dewaxed tomato (Solanum lycopersicum) peels, we id...
Article
Full-text available
Wheat kernel texture, a major trait determining the end-use quality of wheat flour, is mainly influenced by puroindolines. These small basic proteins display in vitro lipid binding and antimicrobial properties, but their cellular functions during grain development remain unknown. To gain an insight into their biological function, a comparative prot...
Article
At present, B cell epitopes involved in food allergy to wheat are known only for a few allergens and a few categories of patients. To characterize the epitopes of different wheat kernel allergens: α-, γ, ω2, and ω5-gliadin, a low-molecular-weight (LMW) glutenin subunit, and a lipid transfer protein (LTP1) recognized by allergic patients and by sens...
Article
One of the objectives of the malting industry is to reduce the energy cost during kilning without major effect on malt quality. In this study, the impact of a low hydration steeping process on lipid transfer protein (LTP1) modifications and β-glucan breakdown was evaluated in low (LH) and high (HH) hydrated malts. LTP1 modifications analyzed by MS/...
Article
Grains of hexaploid wheat, Triticum aestivum (cv. Récital), were collected at 15 stages of development, from anthesis to physiological maturity, 0-700°C days (degree days after anthesis). Two hundred and seven proteins of grain peripheral layers (inner pericarp, hyaline, testa and aleurone layer) were identified by 2-DE, MALDI-TOF MS and data minin...
Article
Wheat endosperm texture is correlated with one major locus, Ha, located on the short arm of chromosome 5D, which comprises several genes among which are two puroindoline genes, Pina and Pinb. In this study, we used two near-isogenic lines, the hard-textured line lacking Pina and the soft-textured line containing both Pina and Pinb wild-type genes....
Chapter
Soluble beer proteins are heat-stable proteins from malted barley. These proteins are mainly composed of proteins that would be involved in the defense of barley against microbial pathogens as a serpin-like cysteine protease inhibitor (protein Z) and lipid transfer proteins (LTPs). These proteins are essential for the formation of beer foam. To bec...
Article
The conformation of puroindoline-a (PIN-a), a protein extracted from wheat endosperm, in free-standing black films has been studied using confocal Raman spectroscopy. This protein is characterized by the presence in its sequence of a unique tryptophan (Trp)-rich domain and of five disulfide bridges stabilizing its three-dimensional structure. PIN-a...
Article
Puroindolines (PINs), basic and cysteine-rich proteins of wheat endosperm, are composed of two proteins, puroindoline-a (PIN-a) and puroindoline-b (PIN-b). Using a monolayer assay at the air/liquid interface, both PIN-a and PIN-b were studied in pure components and mixed with wheat galactolipids, 1,2-di-O-acyl-3-O-(beta-d-galactopyranosyl)- sn-glyc...
Article
Full-text available
Screening of transfer DNA (tDNA) tagged lines of Arabidopsis thaliana for mutants defective in systemic acquired resistance led to the characterization of dir1-1 (defective in induced resistance [systemic acquired resistance, SAR]) mutant. It has been suggested that the protein encoded by the dir1 gene, i.e., DIR1, is involved in the long distance...
Article
The three-dimensional solution structure of a nonspecific lipid transfer protein extracted from maize seeds determined by 1H NMR spectroscopy is described. This cationic protein consists of 93 amino acid residues. Its structure was determined from 1, 091 NOE-derived distance restraints, including 929 interresidue connectivities and 197 dihedral res...
Article
Full-text available
Plant defensins are small basic peptides of 5-10 kDa and most of them exhibit antifungal activity. In a sunflower resistant to broomrape, among the three defensin encoding cDNA identified, SF18, SD2 and HaDef1, only HaDef1 presented a preferential root expression pattern and was induced upon infection by the root parasitic plant Orobanche cumana. T...
Article
The behavior of the two major galactolipids of wheat endosperm, mono- (MGDG) and di-galactosyldiacylglycerol (DGDG) was studied in aqueous dispersion and at the air/liquid interface. The acyl chains of the pure galactolipids and their binary equimolar mixture are in the fluid or liquid expanded phase. SAXS measurements on liquid-crystalline mesopha...
Article
Lipid transfer proteins (LTP) and puroindolines are abundant lipid binding proteins of plant seeds. While LTP are ubiquitous plant proteins, puroindolines are only found in the seeds of plants from the Triticae and Avenae tribes. These proteins display a similar overall folding pattern but different lipid binding properties. The unique and diverse...
Article
Full-text available
Lipid transfer proteins (LTPs) are ubiquitous plant lipid-binding proteins that have been associated with multiple developmental and stress responses. Although LTPs typically bind fatty acids and fatty acid derivatives in a non-covalent way, studies on the LTPs of barley seeds have identified an abundantly occurring covalently modified form, LTP1b,...
Article
To understand the role of the puroindolines (PIN-a and PIN-b) in the defense mechanism and stabilization of lipid films in the gas cell of bread dough, we have isolated the proteins and lipids from wheat seed endosperm and studied their interaction at the air/water interface using a Langmuir trough. The nature and shape of the pressure-area compres...
Article
Full-text available
DIR1, a putative LTP2 protein from Arabidopsis thaliana implicated in systemic acquired resistance in planta, has been crystallized in space group P2(1)2(1)2(1) with one molecule per asymmetric unit. The crystals diffract to a resolution of 1.6 angstroms.
Article
Puroindoline-a (PIN-a) and alpha1-purothionin (alpha1-PTH), isolated from wheat endosperm of Triticum aestivum sp., have been suggested to play a role in plant defence mechanisms against phytopathogenic organisms. We investigated their ability to form pores when incorporated into giant liposomes using the patch-clamp technique. PIN-a formed cationi...