• Home
  • Diana Pelizzari-Raymundo
Diana Pelizzari-Raymundo

Diana Pelizzari-Raymundo
Institut national de la santé et de la recherche médicale - INSERM · Biochemistry

Doctor of Philosophy

About

17
Publications
3,496
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
98
Citations

Publications

Publications (17)
Preprint
Full-text available
Inositol Requiring Enzyme 1 (IRE1) is a bifunctional serine/threonine kinase and endoribonuclease. It is a major mediator of the Unfolded Protein Response (UPR), which is activated during endoplasmic reticulum (ER) stress. Tumor cells experience ER stress due to adverse microenvironmental cues such as hypoxia or nutrient shortage and high metabolic...
Article
Full-text available
The unfolded protein response (UPR) is activated to cope with an accumulation of improperly folded proteins in the Endoplasmic reticulum (ER). The Inositol requiring enzyme 1α (IRE1α) is the most evolutionary conserved transducer of the UPR. Activated IRE1 forms ‘back-to-back’-dimers that enables the unconventional splicing of X-box Binding Protein...
Preprint
Full-text available
Unfolded Protein Response (UPR) and Death Receptor (DR) signalling are cellular stress pathways frequently activated towards pro-tumoral cellular outputs in cancer. Experimental evidence has highlighted functional links between the UPR and signalling by the DR TRAIL-R1/2. Herein, we demonstrate that the UPR sensor IRE1 controls the expression of th...
Preprint
Full-text available
Inositol Requiring Enzyme 1 (IRE1) is a bifunctional serine/threonine kinase and endoribonuclease. It is a major mediator of the Unfolded Protein Response (UPR), which is activated during endoplasmic reticulum (ER) stress. Tumor cells experience ER stress due to adverse microenvironmental cues such as hypoxia or nutrient shortage and high metabolic...
Chapter
IRE1α (inositol-requiring enzyme 1 alpha, referred to IRE1 hereafter) is an Endoplasmic Reticulum (ER) resident transmembrane enzyme with cytosolic kinase/RNAse activities. Upon ER stress IRE1 is activated through trans-autophosphorylation and oligomerization, resulting in a conformational change of the RNase domain, thereby promoting two signaling...
Article
Full-text available
IRE1α (inositol requiring enzyme 1 alpha, referred to IRE1 hereafter) is an Endoplasmic Reticulum (ER) resident transmembrane enzyme with cytosolic kinase/RNAse activities. Upon ER stress IRE1 is activated through trans-autophosphorylation and oligomerization, resulting in a conformational change of the RNase domain thereby promoting two signaling...
Article
The Unfolded Protein response is an adaptive pathway triggered upon alteration of endoplasmic reticulum (ER) homeostasis. It is transduced by three major ER stress sensors, among which the Inositol Requiring Enzyme 1 (IRE1) is the most evolutionarily conserved. IRE1 is an ER-resident type I transmembrane protein exhibiting an ER luminal domain that...
Article
Full-text available
A molecular hallmark in Parkinson's Disease (PD) pathogenesis are α-synuclein aggregates. Cerebral dopamine neurotrophic factor (CDNF) is an atypical growth factor that is mostly resident in the endoplasmic reticulum but exerts its effects both intracellularly and extracellularly. One of the beneficial effects of CDNF can be protecting neurons from...
Article
Full-text available
IRE1α (inositol requiring enzyme 1 alpha) is one of the main transducers of the unfolded protein response (UPR). IRE1α plays instrumental protumoral roles in several cancers, and high IRE1α activity has been associated with poorer prognoses. In this context, IRE1α has been identified as a potentially relevant therapeutic target. Pharmacological inh...
Article
Full-text available
Background: Mutations in CALR observed in myeloproliferative neoplasms (MPN) were recently shown to be pathogenic via their interaction with MPL and the subsequent activation of the Janus Kinase - Signal Transducer and Activator of Transcription (JAK-STAT) pathway. However, little is known on the impact of those variant CALR proteins on endoplasmi...
Article
Full-text available
Background: Mutations in CALR observed in myeloproliferative neoplasms (MPN) were recently shown to be pathogenic via their interaction with MPL and the subsequent activation of the Janus Kinase – Signal Transducer and Activator of Transcription (JAK-STAT) pathway. However, little is known on the impact of those variant CALR proteins on endoplasmic...
Poster
The Unfolded Protein Response (UPR) and its sensor IRE1 have been identified as major regulators of glioblastoma multiforme (GBM) development [1]. IRE1 signalling confers tumour cells with aggressive characteristics including i) the protumoral remodelling of the tumour stroma with immune and endothelial cells and ii) high migration/invasion charact...
Article
Full-text available
BEX3 (Brain Expressed X-linked protein 3) is a member of a mammal-specific placental protein family. Several studies have found the BEX proteins to be associated with neurodegeneration, the cell cycle and cancer. BEX3 has been predicted to be intrinsically disordered and also to represent an intracellular hub for cell signaling. The pro-apoptotic a...
Article
Pyridoxinamine 5'-phosphate oxidases (P(N/M)P oxidases) that bind flavin mononucleotide (FMN) and oxidize pyridoxine 5'-phosphate or pyridoxamine 5'-phosphate to form pyridoxal 5'-phosphate (PLP) are an important class of enzymes that play a central role in cell metabolism. Failure to generate an adequate supply of PLP is very detrimental to most o...
Article
Cerebral Dopamine Neurotrophic Factor (CDNF) is a promising therapeutic agent for Parkinson's disease. As such, there has been great interest in studying its mode of action, which remains unknown. The 3D crystal structure of the N-terminus (residues 9-107) of CDNF has been determined, but there have been no published structural studies on the full-...

Questions

Questions (5)
Question
Hello everyone,
I bought a recombinant protein from a company and the description of the buffer is the following: 20mM Tris, 500mM NaCl, 10% gly, pH 7.4.
Does the "gly" is glycine or glycerol? I couldn't find this information in the data sheet.
Thanks in advance for the help!
Best regards,
Diana
Question
I have these data from an ITC experiment. But I am not sure how to analyze this. The ligand is an IDP protein. So, I think this low affinity is because of that. But I still cant explain why the curve is endothermic.
Could please anyone help me?
Question
I work with a protein that we suspect to be a transcription factor. However,we don't know the specific DNA sequence that it binds. What are the assays available to identify this?
Question
A protein has been identified. We have info on what cells express this protein. How can we identify the receptor of this protein? 
Question
I would like to know experiments to prove my hypothesis.

Network

Cited By