Diana Lupulovic

Scientific veterinary Institute Novi Sad

The paper presents the findings of specific antibodies in the blood sera of donkeys against the following viruses: equine infectious anemia virus (EIAV), African horse sickness virus (AHSV), equine herpesvirus type 1 (EHV-1), equine influenza virus subtype H3N8 (EIV) and equine arteritis virus (EAV). The analyses were conducted during the year 2022...

West Nile virus (WNV) is a Flavivirus that is currently the most spread arbovirus in the world. The virus is maintained in nature in a bird-mosquito enzootic cycle. Humans and horses, as well as donkeys and other equids, are susceptible to WNV infection. There is not many available data on WNV infection in donkeys, since this is still a neglected a...

Avian influenza (AI) is a highly contagious infectious disease of birds that causes high health and economic loses in the poultry industry, and as zoonotic disease, represents a constant threat to human health. During the seasons 2020/2021, and 2021/2022, whole Europe was affected by many outbreaks of AI in both wild birds and domestic poultry, mos...

Machine learning approach for avian influenza virus subtyping using hemagglutinin genomic sequences

The honey bee Apis mellifera is an important beneficial insect recognized by production of honeybee products, having an important role in agricultural production through pollination, and play an equally crucial role in conserving the biodiversity in many natural ecosystems. According to available literature data, in the last more than 20 years, dra...

The results of Serbian national integrated West Nile virus (WNV) surveillance program conducted in 2018 and funded by the Serbian Veterinary Directorate were presented. The WNV surveillance program encompassed the entire territory of Serbia and was conducted by the veterinary service in collaboration with entomologists and ornithologists. The objec...

Background Equine infectious anemia (EIA) is a viral disease, caused by the Equine Infectious Anemia virus (EIAV) belonging to the Retroviridae family, genus Lentivirus. Horses (or equids) infected with EIAV are lifelong carriers and they remain contagious for other horses even in the absence of clinical signs. So far, EIAV infection has been repor...

This article reports on the occurrence and diagnosis of Aujeszky’s disease in a dog. The procedure for isolation and identification of Aujeszky’s disease virus was described. A dog of unknown breed aged about two years died of Aujeszky’s disease after consuming animal offal (internal organs: lungs, spleen, kidneys) fed by the owner after slaughteri...

Here, we report the first whole-genome sequence of an equine arteritis virus (EAV) strain, RS1, isolated from the semen of a Lipizzaner stallion held in the Vojvodina region of Serbia.

West Nile virus (WNV) danas je jedan od najrasprostranjenijih arbovirusa. U posljednja se dva desetljeća WNV infekcije kontinuirano pojavljuju na području Europe u obliku manjih ili većih epidemija i epizootija. U ovom preglednom radu prikazujemo epidemiološke značajke WNV infekcija koje su dokazane na području Hrvatske i Srbije u kontekstu “Jednog...

Lumpy skin disease (LSD) presents constant threat to cattle production because of the high economic losses it causes. Although a lot has been done in the field of improving serological diagnostics of LSD, a reliable determination of immunity status on both herd and individual level remains problematic. In order to test the efficacy of vaccination a...

Infection of cattle with lumpy skin disease virus (LSDV) is very important from the aspect of livestock production. Although it can cause significant economic losses, available serological assays are still not sufficiently efficient and reliable. A 3-day VNT was performed using Madin-Darby bovine kidney (MDBK) cell line and LSDV isolated from clini...

During July 2018, the first clinical case of neurological West Nile virus (WNV) infection was reported in a Belgian sports mare in Belgrade, Serbia. Typical symptoms, such as hypersensitive skin reaction, disorientation, weakness, ataxia and the loss of equilibrium were reported. Detection of WNV IgM antibodies by commercial ELISA in the serum samp...

The aim of this study is to summarize the data on the occurrence, presence and prevalence of some zoonotic flaviviruses, which have been actively circulating in the territory of the Republic of Serbia during past decade. The genus Flavivirus, family Flaviviridae,encompasses vector-transmitted viruses with zoonotic potential. The genus is comprised...

Two strains of classical swine fever virus (CSFV) (SRB/CSFV/1264/2005 and SRB/CSFV/6168/2006), producing serious clinical signs of disease during outbreaks in 2005 and 2006 in Serbia, were isolated on porcine kidney cells, and their complete genomes were determined by next-generation sequencing. This first complete genome characterization of Serbia...

Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the...

Cumulative results of WNV surveillance program in Serbia during 2014, and comparison to the human WNV positive cases reported to the ECDC in 2014. (PDF)

Cumulative results of WNV surveillance program in Serbia during 2015, and comparison to the human WNV positive cases reported to the ECDC in 2015. (PDF)

Description of the sampling locations: Districts names, geographic coordinates and web links. (PDF)

Background Equine arteritis virus (EAV) is responsible for infections in equids. It can spread easily within the horse population and has a major impact on the horse breeding industry. No EAV outbreak has ever been reported in Serbia. To determine whether EAV is nonetheless circulating there, especially in the Vojvodina region, 340 horse serum samp...

During 2015/2016, fecal and intestinal samples from live diseased and/or dead pigs with suspected PEDV and/or TGEV and signs of rotavirus infection were collected from in total seven different farrow-to-finish swine farms located in Northern Serbia region (Vojvodina Province). A total of 14 samples (2 pools per farm) of small intestine with fecal c...

Abstract Mycoplasmal pneumonia caused by Mycoplasma hyopneumoniae (Mhyo) is an important respiratory pig disease that causes severe economic losses to swine industry. It is considered to be most common porcine respiratory disease in commercial swine farms, with low mortality but high morbidity. The aim of research was to evaluate the diagnostic pr...

Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) is a disease aff ecting cattle population of all breeds, categories and age. Th e disease can take diff erent clinical courses, infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis (IPV) being the most common ones. Both diseases, an especially i...

Efforts to detect West Nile virus (WNV) in the Vojvodina province, northern Serbia, commenced with human and mosquito surveillance in 2005, followed by horse (2009) and wild bird (2012) surveillance. The knowledge obtained regarding WNV circulation, combined with the need for timely detection of virus activity and risk assessment resulted in the im...

Bovine Viral Diarrhoea (BVD) is a viral infection of cattle caused by Bovine Viral Diarrhoea Virus (BVDV). Th e most important source of infection is persistently infected and sick cattle, but also other susceptible species. BVDV infection, except for cattle, occurs in sheep, pigs, goats and wild ruminants. Th ese animals can be reservoirs of the v...

Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) are diseases that affect cattle population of all breeds, categories and age. Both diseases, especially infectious bovine rhinotracheitis (IBR), poses severe health threat and causes major economic losses and is considered one of the “most costly” disease in cattle indus...

The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV spec...

Rabies is one of the oldest known zoonotic diseases that has significant impact on public health, but still remains neglected in Serbia. Rabies virus can infect humans and other mammals and causes inflammation of the brain associated with encephalomyelitis and neurological symptoms. In 2010, Veterinary Directorate (national Competent Authority for...

For the first time in Serbia, a small surveillance study was conducted in order to estimate the presence and frequency of occurrence of selected human [adenoviruses (HAdV), noroviruses, (NoV GI, NoV GII) and hepatitis A virus (HAV)], animal [porcine adenovirus (PAdV) and bovine polyomavirus (BPyV)] and zoonotic [hepatitis E virus (HEV)] viruses in...

West Nile virus (WNV) is maintained in nature in an enzootic transmission cycle between birds and mosquitoes, but it also infects many other vertebrates, including humans and horses, in which it can induce severe neurological diseases; however, data about virus circulation in other mammals is scarce. WNV has a history of recent outbreaks in Europe,...

Equine infectious anemia is a consequence of a persistant infection of the horses with Lentivirus. Pathogenesis of the disease is very variable, what can bee seen through a wide range of clinical forms of the disease – from inaparrent infection to death. Diagnostics of EIA is based on clinical symptoms, detection of antibodies and virus. Antibodies...

West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus with zoonotic potential. Virus is maintained in nature in an enzootic transmission cycle between avian hosts and mosquito vectors, but occasionally infects other vertebrates. The infection in horses and humans can be asymptomatic or it can have different clinical manifestations rang...

Introduction: Hepatitis E virus (HEV) infection is rarely reported in industrialized countries, but recent studies have revealed quite variable seroprevalence rates among European populations, including blood donors. In Serbia, very limited data about HEV seroprevalence are available. This study aimed to determine the prevalence of anti-HEV IgG ant...

The aim of the current study was to investigate the presence of antibodies to selected viral disease agents (PRRSV, PPV and CSFV) in hunted wild boars in Vojvodina region. Blood samples from 300 hunted wild boars from hunting ground were collected during the hunting season from October 20011 until March 2012. Presence of anti-PRRSV and anti-CSFV an...

Worlwide, the population density of the wild boar seems to be increasing, which not only means a larger number of hosts available for the transmission of disease, but also a higher contact rate between hosts. Knowledge of diseases circulating in wildlife populations can be important not only for conservation and livestock production but also for pu...

Introduction: This study was conducted to evaluate the seroprevalence of West Nile virus (WNV) in the horse population of northern Serbia. Furthermore, it aimed to provide insight and an updated overview on the circulation of this re-emerging pathogen in this part of southeastern Europe. At the time of manuscript preparation, no clinical cases of...

To establish the presence of West Nile virus (WNV) infection in the animal population in Serbia after the human WNV outbreak, the presence of anti-WNV IgG antibodies was examined by commercial ELISA of blood sera samples of 130 horses collected in 2012 from 6 stables and 1 settlement in Vojvodina Province, northern Serbia. During the blood sampling...

The paper presents the results of the study on equine viral arteritis presence in five horse stables in the territory of Vojvodina. The research encompassed analysis of clinical manifestation of the disease and seroconversion to equine viral arteritis throughout a two-year period (2012 and 2013). Analysis of clinical manifestations of the disease w...

Common carp is the most important freshwater fish in aquaculture in Republic of Serbia. Excess fat deposition in the meat and abdominal cavity of farmed carp can affect carp flesh quality, dressing percentage and consequently restrict the further development of aquaculture production. The reasons for lipid deposition in carp flesh and around viscer...

Serological and virological examination of the presence of human and animal infection caused by West Nile Virus (WNV) as well as the presence of the virus in vectors, which has been conducted during the past few years, confi rmed an active virus circulation in the territory of the Republic of Serbia. Based on the obtained results and anticipated in...

West Nile virus (WNV), a neurovirulent mosquito-transmissible zoonotic virus, has caused recent outbreaks in Europe, including Serbia from August until October 2012. Although humans can be infected, birds are the main natural WNV reservoir. To assess WNV circulation in northern Serbia, 133 wild birds were investigated. These comprised resident and...

The scientific interest in viral diseases of the honeybee (Apis mellifera) has been increasing considerably during the last decade. At least 18 different viruses have been detected in honeybees so far, but six of them are considered to be able to cause severe disease. Clinical signs of disease and suspicions on the bee viruses presence in Serbian a...

This article presents the results of an investigation of equine viral arteritis conducted at six horse stables in the territory of Vojvodina. The research encompassed analysis of clinical manifestation of the disease, seroprevalence and virus detection in native semen samples. Analysis of clinical manifestations of the disease was performed accordi...

Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and comp...

Hepatitis E (HEV) belongs to one of fi ve so far described types of viral hepatitis caused by human agents (hepatitis A, B, C, D and E). The disease is characterized by clinical and epidemiological signs of acute hepatitis and is transmitted primarily by fecal-oral route via contaminated food and water. The infection is mainly detected in the devel...

West Nile virus (WNV), the most widely distributed flavivirus worldwide, has lately reemerged in Europe, causing worrisome outbreaks in humans and horses. Serological analysis by enzyme-linked immunoassay and plaque reduction neutralization test showed for the first time in Serbia that 12% of 349 horses presented specific neutralizing WNV antibodie...

Aujeszky′s disease (MA) is an infectious viral disease, caused by suid alpha herpes virus 1, which displays a very broad host range, except humans and primates. Pigs are the only animal species that can survive the infection with the MA virus, which accounts for its ability to be latently infected. Although pigs are considered a natural host and th...

Hepatitis E virus (HEV) genotype 3 infections in pigs and humans have been lately reported in Europe. In the present study, the prevalence of anti-HEV IgG antibodies in swine was investigated, for the first time, in Serbia by means of an ELISA based on a recombinant open reading frame 2 protein of HEV genotype 3. A total of 315 serum samples from 3...

The paper presents the results of investigations of the presence of the bovine herpesvirus-1 (BHV-1) in samples of blood serum from 106 cows and 107 of their calves (one cow had twins). Blood was sampled from the cows immediately after parturition, and from the calves before feeding on colostrum. The examined cows and their calves originated from 5...

Porcine parvovirus (PPV) is one of the causative agents in reproductive disorders in swine that causes great economic losses. The objective of our research was to determine prevalence of this infection on farms with intensive breeding in 5 municipalities in Vojvodina. The research encompassed 19 herds with a total of 344 blood samples screened for...

Bovine tuberculosis caused by Mycobacterium bovis is a chronicle, infective disease associated with formation of specific inflammatory granulomas. Infection usually remains subclinical for a long period, but even when the clinical are present, they are not pathognomic. Bovine tuberculosis can spread to humans through inhalation of infectious drople...

Bovine viral diarrhoea (BVD) is a worldwide viral cattle disease. Percentage of seropositive animals, especially in the countries with intensive cattle breeding ranges from 60-90%. This infection on our epizootiology area, as well as on area in our surrounding, was for the first time described by OEuriåkoviñ et al. (1966) based on clinical pictures...

Bovine herpesvirus type 1 infection (IBR/IPV virus) presents the most spread infection in nowadays cattle breeding. Bovine herpesvirus-1 (BHV-1) may be an agent of serious health disorders causing great economic losses. Since the largest number of cattle in Southern Baåka and Srem district are raised in small herds (what may considerably influence...

Intrauterine infection of the fetus occurs if a pregnant non-vaccinated sow is infected with the virus of classical swine plague (CSF). The infection of the fetus results in the occurrence of viremia and the distribution of the virus in fetal tissue is similar to the distribution which is established in post-natal infected swine. The objective of t...

Parvoviral infection of swine is a disease which is manifested in reproductive disorders in sows and gilts in the form of anestria, premature births, miscarriages, mummified fetuses, the birth of poorly vital piglets and/or a reduced number of piglets in the litter. The infection is present in farms with intensive breeding conditions in the form of...

The program of curbing and outrooting classical swine fever (CSF) in our country has for years been based on the vaccination of all categories of swine with the Kina (K) strain of the CSF virus, and, after the establishment of the source of infection, with the non-harmful removal of the infected swine herd, as well as on the implementation of all m...

The ELISA technique is one of the most commonly used laboratory-diagnostical methods for diagnosing many infective diseases in humans and animals today. Due to its high specificity and sensitivity this technique is considered a method of choice in diagnosing many viral diseases. The phases and components of the ELISA technique are constantly improv...

It has been proposed that avian influenza virus causes significant health problems in birds, other animals and humans. In birds the disease can be expressed in subclinical form or is accompanied with mild respiratory problems or lower egg production. It can be also manifested in an acute form with high mortality. Due to unspecific disease symptoms,...