Dhaneshwar Suneela

• M. Pharm. Ph D. (Pharm. Chem.)
• Director at Amity Institute of Pharmacy, Amity University Maharashtra Mumbai MS India

The present study utilized Analytical Quality by Design (AQbD) approach to develop a stability‐indicating high‐performance liquid chromatography (HPLC) method for estimating evogliptin tartrate using design expert software. The key parameters were methodically optimized, contours were plotted, and stability was evaluated using various forced degrad...

Advances in Diagnostics and Immunotherapeutics for Neurodegenerative Diseases delves into the intricate mechanisms underlying neurodegenerative disorders and highlights cutting-edge diagnostic methods and innovative immunotherapeutic strategies. This comprehensive book addresses several key themes crucial to understanding and combating these debili...

a amity Institute of pharmacy, lucknow, amity university uttar pradesh, noida, uttar pradesh, India; b amity Institute of pharmacy, amity university maharashtra, mumbai, maharashtra, India; c centre for advanced research (cFar), Faculty of medicine, King george's medical university (Kgmu), uttar pradesh, lucknow, India ABSTRACT Background: Intestin...

Inflammatory bowel disease (IBD), represented by Crohn’s disease (CD) and ulcerative colitis (UC), is a chronic inflammatory disorder of the gastrointestinal tract (GIT) characterized by chronic relapsing intestinal inflammation, abdominal pain, cramping, loss of appetite, fatigue, diarrhoea, and weight loss. Although the etiology of IBD remains un...

Background Presently, only four drugs have been approved by FDA for Alzheimer’s disease (AD). A drug repurposing approach can be fruitful in searching for promising candidates for AD. Objective The objective of the work was to evaluate the neuroprotective effect of levetiracetam (LEV) in combination with berberine (BER) in scopolamine-induced cogn...

Pancreatic ductal adenocarcinoma (PDAC) remains a significant public health challenge and is currently the fourth leading cause of cancer-related mortality in developed countries. Despite advances in cancer treatment, the 5-year survival rate for patients with PDAC remains less than 5%. In recent years, neoadjuvant therapy (NAT) has emerged as a pr...

Alzheimer's disease, Parkinson's disease, Amyotrophic lateral sclerosis, Huntington's disease, and Friedrich ataxia are all incurable neurodegenerative diseases defined by the continuous progressive loss of distinct neuronal subtypes. Despite their rising prevalence among the world's ageing population, fewer advances have been made in the concurren...

Background: Evogliptin tartrate is a novel dipeptidyl peptidase (DPP-4) inhibitor very recently introduced into the market as an oral hypoglycemic drug. Objective: The literature review has revealed no reports of stability-indicating analytical methods so far for evogliptin tartrate. So, the goal of this study was to develop and validate a stabi...

Attention-deficit hyperactivity disorder (ADHD) is a neurobiological and neurodevelopmental disorder with an idiosyncratic genetic base. ADHD presents various characteristics, such as inattention, hyperactivity, and impulsivity. Over the period, ADHD leads to noticeable functional disability. A five- to ten-fold progressed risk of disorder developm...

Experimental evidence supports the fact that changes in the bowel microflora due to environmental or dietary factors have been investigated as implicating factors in the etiopathogenesis of inflammatory bowel disease (IBD). The amassing knowledge that the inhabited microbiome regulates the gut physiology and immune functions in IBD, has led researc...

Acromegaly is described by oversecretion of growth hormone (GH) and, subsequently, insulin-like growth factor 1 (IGF-1), ascribed in most cases to a pituitary adenoma. This disease presents a progressive disfigurement, along with other systemic manifestations, which altogether diminishes the quality of life in the patients. Prolonged exposure to ab...

Objective: The level of precursors involved in the biosynthesis of glycosaminoglycan (GAG), glucosamine synthase, and N-acetyl glucosamine (NAG), are significantly reduced in inflammatory bowel disease (IBD). This results in deficient GAG content in mucosa, which eventually disrupt the gut wall integrity, provoking abnormal immunological responses....

A precise, sensitive, specific and accurate stability indicating densitometric method was developed and validated for alpha-lipoic acid (ALA) in bulk and capsule dosage form. The study employed pre-coated silica gel 60F 254 TLC plates as stationary phase and toluene: chloroform: methanol: formic acid (5:3:1:0.05; v/v/v/v ) as mobile phase. The deve...

Background Adverse effects induced by upper GIT release of mycophenolic acid (MPA) and its prodrug mycophenolate mofetil (MMF) have created a great deal of concern in the treatment of inflammatory bowel disease (IBD). Objective The goal of this work was to create a polymer-based prodrug (MDS) by attaching MPA to dextran to enable colon-targeted dr...

Artificial Sweeteners (AS) were first introduced as food additives or sugar substitutes more than a century ago having the intention of supplying sweet taste without the high caloric content of sugar. AS are employed in place of sucrose (table sugar) with the view of providing sweetness to foodstuffs and bottled drinks. The end-users, along with pr...

Background Mucormycosis is a genuine, however uncommon shrewd fungal contamination that spreads quickly, and subsequently brief analysis and treatment are important to stay away from high pace of mortality and morbidity rates. Mucormycosis is brought about by the inward breath of its filamentous (hyphal structure) fungus particularly in the patient...

Background Pro-inflammatory mediators such as tumor necrosis factor-alpha (TNF-α), interleukin (IL), and oxidative stress are crucial players in the pathophysiology of inflammatory bowel disease (IBD) that contribute in perpetuating intestinal inflammation. Targeting them presents a novel approach in disease management. In the present study, the po...

Rheumatoid arthritis [RA] is a painful disorder that causes inflammation in synovial membrane followed by damage to cartilage and bone. Several therapies are available for its management that include anti-inflammatory and disease- modifying antirheumatic drugs. Due to the severe side effects associated with them, phytotherapy may serve as a promisi...

Among the different polymers derived from natural origin, dextrans are one of the widely explored classes in the field of drug delivery. This chapter describes the chemistry, physicochemical properties, and reactivity of dextrans. An elaborative account of various drug-dextran conjugates, dextran hydrogels, dextran-micellar nanocarrier systems, dex...

Background Alzheimer's disease (AD) is a neurodegenerative disease characterized by impairment of memory, disturbances in reasoning, planning, language and perception. Currently, there are only four drugs approved by US-FDA for AD; therefore, there is an extensive need for new drug development. The drug repositioning approach refers to the developm...

Drug repositioning or repurposing is a revolutionary breakthrough in drug development that focuses on rediscovering new uses for old therapeutic agents. Drug repositioning can be defined more precisely as the process of exploring new indications for an already approved drug while drug repurposing includes overall re-development approaches grounded...

ABSTARCT Aceclofenac is a nonsteroidal antiinflammatory drug that is used to relieve pain and inflammation in arthritic conditions like osteoarthritis, rheumatoid arthritis and ankylosing spodylitis. Aceclofenac is known to induce erosion and ulcers in the gastrointestinal tract but in contrast to other nonsteroidal anti-inflammatory agents, it has...

The long-course treatment of tuberculosis with isoniazid (INH) leads to hazardous side effects on liver and poor patient compliance. To overcome these toxic effects caused by INH, a unique hepatoprotective co-drug platform was developed by tethering INH with ursodeoxycholic acid (UDCA) –an antioxidant bile acid for possible synergistic outcome. INH...

The enigmatic coronavirus outburst on December 31, 2019, originated from Wuhan city of China, is now declared as Coronavirus disease (COVID-19) by World Health Organization (WHO). The causative agent is highly contagious, and its rapid blowout affects almost every country of the world, endangering thousands of lives. Recently, WHO has raised the CO...

The prolonged use of isoniazid (INH) – a highly effective drug in the treatment of tuberculosis – causes fatal liver injury. In order to overcome this adverse effect, a unique amide codrug was designed by covalently linking INH with sulfur-containing antioxidant- alpha-lipoic acid for possible hepatoprotective and antimycobacterial effect. Co-drug...

Boswellia serrata (Burseraceae) is the most ancient and respected herbs in Ayurveda. In Unani system of medicine, oleo-gum resin of Boswellia serrata named Kundur played a prime ingredient role in modern quality perfumes. The gum is used as a remedy for treatment of illness especially skin diseases and rheumatism in Indian system of medicine (Sidha...

Objective: The objective of the present work was to develop validated stability-indicating high-performance thin-layer chromatographic method for simultaneous estimation of formoterol fumarate dihydrate (FFD) and fluticasone propionate (FP) in bulk drug and pharmaceutical dosage form. Methods: Pre-coated silica gel aluminum plates 60 F-254 were use...

AIM To overcome the hazardous effects on liver caused by long-term use of antitubercular agent isoniazid (INH) by developing a novel hepatoprotective prodrug strategy by conjugating INH with aminothiols as antioxidant promoities for probable synergistic effect. METHODS INH was conjugated with N-acetyl cysteine (NAC) and N-(2)-mercaptopropionyl gly...

The extraction of crude drugs by using different solvents provides polarity-based fractions containing specific types of secondary metabolites. Averrhoa carambola L. fruits were extracted and fractionated, and petroleum ether extract was processed by fatty acid methyl ester (FAME) technique for characterization by gas chromatography-mass spectromet...

Aim: To design colon-targeted codrugs of mycophenolic acid (MPA) and aminosugars as a safer option to mycophenolate mofetil (MMF) in the management of inflammatory bowel disease. Methods: Codrugs were synthesized by coupling MPA with aminosugars (D-glucosamine and D-galactosamine) using EDCI coupling. The structures were confirmed by infrared ra...

Morphological and metabolic aberrations in the liver caused by long-term use of anti-tubercular agent isoniazid (INH) have been an issue of great concern in tuberculosis treatment. To resolve this issue, a novel hepatoprotective prodrug strategy was developed by combining the antioxidant property of phenolic acids with INH moiety for probable syner...

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by restricted movements of joints of hand, feet, elbow, knees and neck but principally the synovial joints. Though etiopathology is not exactly known, treatment paradigms are evolving to provide a tighter control over symptoms and disease progression. Current trend is introduct...

AIM To develop a colon-targeting bioreversible delivery system for β-boswellic acid (BBA) and explore utility of its prodrugs in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats. METHODS Synthesis of 4 co-drugs of BBA with essential amino acids was achieved by CDI coupling, followed by their spectral characterization. In vitro ki...

Incomplete absorption and poor oral bioavailability of hydroxychloroquine (HCQ) as well as gastrointestinal erosions of NSAIDs have attributed to major hurdles in the treatment of rheumatoid arthritis. To overcome these hurdles, we designed self- immolative dual delivery approach for disease modifying antirheumatic hydroxychloroquine and aryl propi...

Mutual amide prodrugs of β-boswellic acid (BBA) with L-glutamine and L-cysteine were synthesized for the management of inflammatory bowel disease. Conjugation with amino acids improved the hydrophilicity of BBA (log P: 4.9 to5.3) to enable efficient delivery to colon. Prodrugs were stable in 0.05M HCl buffer (pH 1.2) and stomach homogenates. Neglig...

Background: Recent immunologic data implicates involvement of mucosal immune cells of the intestine like eosinophils and mast cells to be functionally involved in the pathogenesis of UC. Mast cell activation is followed by increased secretion and elevated tissue concentration of histamine. Inhibition of mucosal histamine release in colon may be an...

Intrduction: The present study reports validated stability indicating high performance thin layer chromatographic (HPTLC) method of analysis for estimation of an immunosuppressant drug; mycophenolate sodium in bulk drug and formulations. Method: Silica gel pre-coated aluminium plates 60F254 were used as stationary phase. Various mobile phases were...

N-acetylcysteine (NAC) exhibits diverse pharmacological effects due to the free radical scavenging ability of the reduced thiol. Extensive deacetylation in liver, high protein binding and poor permeability results in low bioavailability of NAC. These setbacks are also the contributing factors in failure to confirm the benefits of NAC in prevention...

Background: The mucolytic N-acetylcysteine (NAC) is used to control the excessive mucus secretion if mucus is the underlying cause of broncho-constriction. Its major drawbacks are poor bioavailability due to extensive first pass effect, poor lipophilicity, high protein binding and offensive odor. Methods: For minimizing above shortcomings of NAC, i...

Methyl N-unprotected -amino acid esters are important intermediates in organic synthesis and can be conveniently prepared by a Fisher-type esterification. Microwave (MW)-assisted organic synthesis has evolved as an important technique in green chemistry and has shown high impact in enhancing the rates of reaction and speeding up library synthesis....

Background: Rheumatoid arthritis (RA) is an autoimmune disease that results in a chronic, systemic inflammation that may affect many tissues and organs, but principally the synovial joints. The tendency for joint destruction is greatest in the early stages of disease hence current trend is to introduce a disease-modifying anti-rheumatic drug (DMAR...

Background: The mucolytic N-acetylcysteine (NAC) is used to control the excessive mucus secretion if mucus is the underlying cause of broncho-constriction. Its major drawbacks are poor bioavailability due to extensive first pass effect, poor lipophilicity, high protein binding and offensive odor. Methods: For minimizing above shortcomings of NAC...

Elevated concentration of any or all types of lipids in the plasma including hypertriglyceridemia and hypercholesterolemia leads to atherosclerotic cardiovascular disease. Effective medication needs multiple drug therapy as recommended cholesterol and triglyceride levels are difficult to achieve by monotherapy and frequently requires the use of mor...

The objective of present work was to develop a validated simple, precise, accurate and specific high performance thin layer chromatographic method for estimation of fexofenadine hydrochloride as a bulk drug and in tablet dosage form. The stationary phase used was precoated silica gel aluminium plates 60 F254 with 250 µm thickness. The mobile phase...

We have reported the synthesis, characterization¬¬¬, in vitro release profile and preliminary pharmacological investigations of an antioxidant mutual prodrug of diacerein with thymol in our earlier communication. The present work reports the results of in vivo release studies and extensive pharmacological evaluation of this prodrug in collagenase-...

Recent WHO guidelines recommend combination antihypertensive therapy as the first-line therapy for hypertension because monotherapies are often abortive and may lead to delayed BP control, increased risk of stroke and death. Present work was focused on designing a thioester- linked mutual prodrug of captopril and as a combination therapy. The main...

Objective: This paper describes a new, simple, precise, accurate and specific HPTLC method for estimation of voglibose as a bulk drug and in tablet dosage forms. Methods: Chromatographic separation of the drug was performed on aluminum plates pre-coated with silica gel 60 F254 as the stationary phase and a mobile phase comprising of toluene: ethyl...

Disease modifying antirheumatic drugs (DMARDs) is a category of drugs which is used as medication in various arthritic conditions to arrest the progression of disease along with relief from pain. About 83% of population worldwide uses DMARDs. Withdrawal of COX-2 inhibitors because of cardiovascular side effects and short-term action associated with...

Despite the advent of biological products, such as anti-tumor necrosis factor-α monoclonal antibodies (infliximab and adalimumab), for treatment of moderate to severe cases of inflammatory bowel disease (IBD), most patients depend upon aminosalicylates as the conventional treatment option. In recent years, the increased knowledge of complex pathoph...

Bioprecursor by definition is a type of prodrug that is designed to tackle pharmacetical, pharmacokinetic or pharmacodynamics shortcomings of a drug that limits its clinical use. A retrometabolic approach is used to design bioprecursors which upon activation by either phase-I or phase-II metabolic enzymes result into an active metabolite. A bioprec...

Photodynamic therapy (PDT) is a promising local treatment modality based on the selective accumulation of a photo sensitizer in malignant tissues and the subsequent irradiation with laser light. Photo sensitizer is a substance that sensitizes an organism, cell, or tissue to light. It is an inherent part of PDT which, when absorbed by cancerous cell...

We have reported the synthesis, characterization, in vitro release profile and preliminary pharmacological investigations of an antioxidant mutual prodrug of diacerein with thymol in our earlier communication. The present work reports the results of in vivo release studies and extensive pharmacological evaluation of this prodrug in collagenase- ind...

Azo prodrugs of aminosalicylates viz: 5-aminosalicylic acid and 4-aminosalicylic acid were synthesised using phenols as colon- targeting carriers for management of inflammatory bowel disease. The structures were confirmed by spectral and elemental analysis. These azo- linked prodrugs showed increased hydrophilicity which prevented their absorption...

Azo prodrugs of aminosalicylates viz: 5-aminosalicylic acid and 4-aminosalicylic acid were synthesised using phenols as colon- targeting carriers for management of inflammatory bowel disease. The structures were confirmed by spectral and elemental analysis. These azo- linked prodrugs showed increased hydrophilicity which prevented their absorption...

Novel mutual prodrugs of biphenylacetic acid were designed as a promising gastro-protective alternative to fenbufen. Biphenyacetic acid was covalently linked with two non-essential amino acids (D-phenylalanine and glycine) possessing wound healing, analgesic, and anti-inflammatory properties. The prodrugs exhibited good stability in stomach homogen...

Novel carrier-linked azo prodrugs of 4 and 5-aminosalicylic acids (4-ASA and 5-ASA respectively) using the same drugs as carriers in different permutations and combinations were designed for targeting colon affected with inflammatory bowel disease (IBD).Improved hydrophilic nature of the prodrugs assisted in minimizing their absorption in upper GIT...

We report here in vitro and in vivo stability and release studies performed on prodrug of biphenylacetic acid with L-tryptophan (BPTP) which was designed as a gastro-protective option for the latter and its bioprecursor fenbufen. Chemical stability of the prodrug was investigated in aqueous buffers in the range of acidic and weakly alkaline pH and...

Diacerein and its active metabolite rhein are promising disease modifying agents for osteoarthritis (OA). Boswellic acid is an active ingredient of Gugglu; a herbal medicine commonly administered in osteoarthritis. Both of them possess excellent anti-inflammatory and anti-arthritic activities. It was thought interesting to conjugate rhein and boswe...

Sterically hindered esters or esters of drugs with macromolecular carriers like dextran and cyclodextrin find wide applicability in colon-targeted delivery. We report here synthesis, in vitro release kinetics of macromolecular prodrug of 4-aminosalicylic acid (4-ASA) with β-cyclodextrin and its extensive pharmacological evaluation in 2, 4, 6- trini...

Involvement of oxidative stress, leading to chondrocyte senescence and cartilage ageing has been implicated in the pathogenesis of osteoarthritis (OA). New efforts to prevent the development and progression of OA include strategies and interventions aimed at reducing oxidative damage in articular cartilage using antioxidants as adjuncts to conserva...

In an attempt to minimize the ulcerogenic potential and associated gastro-intestinal toxicity of bioprecursor fenbufen and its active metabolite biphenyl acetic acid, carrier-linked chimeric derivatives of the latter were designed and synthesized using amino acids as the promoities. DCC coupling method was used for the synthesis of these amides. Th...

Rhein, an active metabolite of diacerein, down-regulates the gene-expression and production of pro-matrix metalloproteinases and up-regulates the tissue inhibitors of metalloproteinase-1 production. The therapeutic effects of diacerein on osteoarthritis are, at least in part, due to the chondroprotective effect of rhein. Boswellic acid is a specifi...

Present work was inspired by an interesting finding of Raithel et al. (11) about remission of steroid-dependent, chronically active ulcerative colitis (UC) in a patient, after treatment with a combination of fexofenadine, disodium cromoglycate and an amino acid-based formula. Literature reports involvement of mast cells activation and increased his...

Chondromodulating chimeric prodrugs of diacetylrhein were synthesized with an objective of potentiating its moderate anti-inflammatory effect and optimizing its hydrophilic/lipophilic balance by conjugating it with essential amino acids through a bioreversible amide linkage. In vitro release in HCl buffer (pH 1.2) showed insignificant release of di...

Sterically hindered esters or esters of drugs with macromolecular carriers like dextran and cyclodextrin find wide applicability in colon-targeted delivery. We report here synthesis, in vitro release kinetics of macromolecular prodrug of 4-aminosalicylic acid (4-ASA) with β-cyclodextrin and its extensive pharmacological evaluation in 2, 4, 6- trini...

Fenbufen, a bioprecursor prodrug owes its analgesic and anti-inflammatory activity to its active metabolite [1, 1'-biphenyl]-4-acetic acid (BPAA). It is used in the treatment of rheumatoid arthritis and osteoarthritis. Gastrointestinal complaints are the most frequently reported side effects of fenbufen and its major drawback is involvement of more...

Even though sulfasalazine is drug of choice for treatment of ulcerative colitis (UC), 5-aminosalicylicacid (5-ASA)-induced pancreatitis and sulfapyridine-induced hepatitis are well documented in the literature. 4-aminosalicylic acid (4-ASA) is a promising but unexplored alternative, offering a lower risk of pancreatitis than 5-ASA. According to rec...

We report here design and development of prodrug of aceclofenac with L-tryptophan aiming at reducing its ulcerogenic propensity and enhancing its anti-inflammatory potential using chimeric approach. The amide prodrug was synthesized by routine DCC coupling and its structure was established and confirmed by spectral analysis. The release kinetics wa...

Earlier colon was considered as a black-box, acting as a site for production and temporary storage of excreta and responsible for absorption of electrolytes and water. But, with the discovery of sulfasalazine as colon-specific prodrug, the promising and challenging issue of treating local pathologies was presented with colon as an organ of signific...

There is a great emphasis on research to discover methods aimed at enhancing the efficacy of drugs and reducing their toxicity and unwanted side effects. Prodrugs are biologically inactive compounds that are converted to actual drug molecule, through biotransformation, that combine with the receptors to produce the biological action. Prodrugs can t...

Prodrug approach using diglyceride as a promoiety is a promising strategy to improve bioavailability of poorly absorbed drugs and the same was explored in the present work to improve oral bioavailability of norfloxacin; a second generation fluoroquinolone antibacterial. The prodrug was synthesized by standard procedures using dipalmitine as a carri...

Aceclofenac is a nonsteroidal anti-inflammatory drug used to relieve inflammation and associated pain in various forms of arthritis and has become popular due to its stimulatory effects on cartilage matrix synthesis even though it is known to induce erosion and ulcers in the gastro-intestinal tract. In the present work we synthesized a mutual prodr...

A gastro-sparing diacerein mutual prodrug was designed and synthesized by linking it to D-glucosamine as a promoiety with an objective of increasing the potential of its anti-inflammatory activity and improving its hydrophilic characteristics so as to make its parenteral administration feasible. Diacerein was linked with D-glucosamine through amide...

Mesalazine is widely used systemically or topically to achieve and maintain remission in inflammatory bowel disease. Latentiated derivatives of mesalazine having L-histidine and L-tyrosine covalently linked via an amide linkage were designed, developed and screened in 2, 4, 6-trinitrobenzenesulphonic acid-induced experimental colitis in rats. Incub...

The objective of the present work was to investigate the targeting potential of L-glutamine utilizing concept-based mutual prodrug design for the delivery of meselamine to colon. Meselamine was linked with L-glutamine through azo linkage and was subjected to in vitro stability studies in aqueous buffers of varied pH range and rat fecal matter. 84.7...

We have reported synthesis and in vitro release studies of a gastro-sparing mutual prodrug of diacerein with the nutraceutical aminosugar; D-glucosamine as a promoiety. The results of preliminary pharmacological screening were promising which prompted us to evaluate its potential for long-term use in the safer management of osteoarthritis. This wor...

Colon-specific mutual azo prodrugs of 5-aminosalicylic acid with essential amino acids were synthesized for the management of inflammatory bowel disease. The structures were confirmed by elemental and spectral analyses. 85-88% release of 5-aminosalicylic acid was achieved in rat fecal matter with half-lives ranging from 140 to 160 min, following fi...

Mutual amide prodrugs of 4-aminosalicylic acid with D-phenylalanine and L-tryptophan were synthesized for targeted drug delivery to the inflamed gut tissue in inflammatory bowel disease. Stability studies in aqueous buffers (pH 1.2 and 7.4) showed that the synthesized prodrugs were stable in both the buffers over a period of 10 h. In rat fecal matt...

Duloxetine hydrochloride is a potent dual reuptake inhibitor of serotonin and norepinephrine used to treat major depressive disorders. The present work describes a simple, precise and accurate HPTLC method for its estimation as bulk and in tablet dosage form. The chromatographic separation was carried out on precoated silica gel 60 F254 aluminium p...

Simvastatin is a selective HMG-CoA reductase inhibitor and ezetimibe has lipid-lowering activity. Both are potential anti-lipidemic agents used in combination to reduce the amount of cholesterol and triglycerides in systemic circulation. This paper describes a simple, precise, and accurate HPTLC method for simultaneous estimation of the compounds a...

SUMMARY Atorvastatin calcium is a selective HMG-CoA reductase inhibitor and ezetimibe has lipid-lowering activity. Both are potential anti-lipidae- mic agents used in combination to reduce the amount of cholesterol and triglycerides in systemic circulation. This paper describes a simple, precise, and accurate HPTLC method for simultaneous quantific...