Devit Purwoko

National Research and Innovation Agency (BRIN) · Research Center for Genetic Engineering-Research Organization for Life Sciences and Environment

Sago plant (Metroxylon sagu Rottb.) is one of the most carbohydrate-producing plants in the world. Microsatellites or simple sequence repeats (SSRs) play an important role in the genome and are used extensively compared to other molecular markers. For the first time, we are exploiting data expressed sequence tags (EST) of sago plants to identify an...

Sago palm (Metroxylon sagu Rottb.) is a carbohydrate-producing plant. It produces a starchy, edible pith or core within its trunk, which is the primary portion used for human consumption. To date, little has been investigated about the diversity of sago palm plants in Bengkulu, Indonesia. In this study, DNA was extracted from young leaves collected...

Recalcitrancy is the main problem in rice anther culture, especially in an Indica subspecies. It is well known that recalcitrancy is genotype dependent, and the effort to tackle this problem is mostly in the manipulations of media. Uncovering the genes responsible for recalcitrancy will benefit to enhance the success of this technique to accelerate...

Breeding is one method to develop better varieties. This method can be accelerated by using haploid technology. The availability of haploid inducer lines in maize makes in-vivo techniques preferred, especially for temperate maize. Developing in-vitro through anther culture in haploid breeding is still needed since haploid inducer lines are still li...

Curcuma aeruginosa Roxb. is an Indonesian traditional medicinal plant of the Zingiberaceae family. C. aeruginosa is known to have anticancer activity, especially in the rhizomes. Despite many studies on the phytochemical content of this plant with antioxidant and anticancer activity, transcriptomic studies are still limited in terms of genetic info...

The content of polysaccharides, polyphenols, proteins, and RNA compounds is the main problem often found in Plants DNA isolation, which inhibit the process of DNA isolation. Comparing the methods of plant DNA isolation is necessary for obtaining the DNA with good quality, purity, high concentration and efficiency time and cost. This study aimed to...

The assembly of dihaploid rice plants through anther culture was constrained due to the recalcitrant properties. A comprehensive investigation of gene expression patterns among rice varieties with recalcitrant and non-recalcitrant anthers will help to understand the cellular mechanisms and biological processes of recalcitrant properties in rice ant...

Oil palm empty fruit bunches (OPEFB) are the lignocellulosic complex organic waste material from palm oil mills that is cheap, environmentally friendly, and abundant in Indonesia. Slow degradation of OPEFB becomes a problem for oil palm plantations. OPEFB which has decayed naturally for 6 months, 1 year, and 2 years were obtained from the Oil Palm...

Temu ireng (Curcuma aeruginosa) is used as a traditional herb medicine in Indonesia. Expressed Sequence Tags (EST) of C. longa was used because there were no genome data of C. aeruginosa. This study aimed to recognize the diversity of SSR characteristic and to develop a primer for C. aeruginosa genetic diversity. The EST was preprocessed and analyz...

The availability of high-quality seeds is now a necessity. This is due to a government program to replace oil palm trees in smallholder plantations with high quality seeds. An efficient protocol to produce a large number of embryos is needed. To increase the number of embryogenic callus production, the callus proliferation experiment was carried ou...

Screening and Identification of Ligninolytic Microbes in the Natural Decomposition of Oil Palm Empty Fruit Bunch ABSTRACTOPEFB (oil palm empty fruit bunch)could potentially be utilized as organic fertilizer or animal feed through composting. Information on microorganisms that play important roles in the natural decomposition of OPEFB is to date not...

Obtaining high-quality RNA is very important at an early stage of molecular biology research. To isolate RNA, high skill and caution are required in following laboratory procedures because RNA is easily degraded, especially samples from plant tissue culture. One of the parameters used to check the total RNA quality is RIN (RNA Integrity Number). Th...

Sago palm (Metroxylon sagu Rottb.) is one of the most productive carbohydrate-producing crops. Unfortunately, only limited information regarding sago palm genetics is available. This study aimed to develop simple sequence repeat (SSR) markers using sago palm NGS genomic data and use these markers to evaluate the genetic diversity of sago palm from...

TKKS (tandan kosong kelapa sawit) berpotensi untuk dimanfaatkan kembali dengan cara pengomposan menjadi pupuk organik atau pakan ternak. Pembuatan kompos memerlukan mikroba pengompos agar proses pengomposan berjalan lebih cepat dan hasilnya lebih baik. Pada proses pengomposan, yang sering menjadi perhatian adalah bagaimana memahami proses degradasi...

Sago genome sequencing analysis is still very limited. This study is a preliminary study of sago sequence analysis obtained from NGS technology to understand and identify new genetic sequences that have homology to genes in the NCBI database. Sequences were analyzed using Blast2Go to determine the genetic function annotation, putative gene identifi...

WEB-based bioinformatic analysis on partial genome sequence of Sago (Metroxylon sagu Rottb.)ABSTRACTSago genome sequencing analysis is still very limited. This study is a preliminary study of sago sequence analysis obtained from NGS technology to understand and identify new genetic sequences that have homology to genes in the NCBI database. Sequenc...

ABSTRAK Kelapa sawit (Elaeis guineensis) merupakan tanaman penghasil minyak nabati terbesar di dunia dengan kuantitas per-unit lahan terbesar. Indonesia merupakan salah satu penghasil komoditas kelapa sawit terbesar di dunia. Peningkatan luas areal perkebunan sawit meningkat tiap tahunnya sejalan dengan meningkatnya produksi CPO. Dengan demikian li...

Sago Palm (Metroxylon sagu Rottb.) is regarded one of most produced carbohydrate plant in the world. However, only limited genomic information progress has been made especially in DNA markers. Microsatellites as well knew SSRs has an important role on the genome and many used than another molecular marker. For the first report, we have exploitation...

ABSTRACT National rubber productivity is lower than other rubber producing countries in the world. The DNA marker-based plant breeding program is required to increase latex production and other superior characters. Breeding process by identifying genetic diversity can be done using AFLP marker. The aim of this study was to analyze the phylogenetic...

Phylogenetic Analysis of Rubber Tree Clones using AFLP (Amplified Fragment Length Polymorphism) MarkerNational rubber productivity is lower than other rubber producing countries in the world. The DNA marker-based plant breeding program is required to increase latex production and other superior characters. Breeding process by identifying genetic di...

Curcuma xanthorrhiza Roxb, which is well-known as Java turmeric, has been extensively used in pharmaceutical industries in Indonesia. In spite of this commercial value, the identity of this species is commonly mistaken from other similar orange rhizomes Curcuma. Correct identity of these species is vital in pharmaceutical industries. The objective...

Curcuma xanthorrhiza Roxb, which is well-known as Java turmeric, has been extensively used in pharmaceutical industries in Indonesia. In spite of this commercial value, the identity of this species is commonly mistaken from other similar orange rhizomes Curcuma. Correct identity of these species is vital in pharmaceutical industries. The objective...

Various kinds of microorganism identification process both conventional and modern readly available and commonly used by various laboratories. Conventional methods such as staining and morphology are so widely used in various laboratories. However, identification method rely on DNA sequence is now starting to be used in many laboratories. Collabora...

Study on bioactive compounds of curcuma and their effect on human health have been carried out in many research institutions. Curcuma xanthorrhiza, or Java turmeric, has been used in pharmaceutical industries in Indonesia. In spite of this commercial value, the identity of this species is commonly mistaken from other similar orange rhizomes Curcuma...

Study on bioactive compounds of curcuma and their effect on human health have been carried out in many research institutions. Curcuma xanthorrhiza, or Java turmeric, has been used in pharmaceutical industries in Indonesia. In spite of this commercial value, the identity of this species is commonly mistaken from other similar orange rhizomes Curcuma...