Devin Wahl

Devin Wahl
Colorado State University | CSU · Department of Health and Exercise Science

PhD

About

39
Publications
30,184
Reads
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1,268
Citations
Introduction
I am an aging researcher and post-doctoral fellow in the Healthspan Biology Lab at Colorado State University. I am interested in identifying interventions to combat age-related disease, improve healthspan, and increase lifespan. I have a particular interest in neurodegenerative disease and I am studying interventions (e.g., nutritional or pharmacological) that improve cognition and brain health during aging.
Additional affiliations
July 2018 - March 2019
The University of Sydney
Position
  • Research Associate
May 2013 - May 2015
National Institute on Aging
Position
  • PostBac IRTA
September 2012 - December 2012
Colorado College
Position
  • Research Assistant
Description
  • Neuroscience tutor and teaching assistant, Colorado College. Responsibilities: scheduling and holding tutoring sessions for fellow students and providing additional assistance for fellow classmates.
Education
July 2015 - July 2018
The University of Sydney
Field of study
  • Medicine and neuroscience

Publications

Publications (39)
Article
Full-text available
Aging is a powerful risk factor for the development of many chronic diseases including dementia. Research based on disease models of dementia have yet to yield effective treatments, therefore it is opportune to consider whether the aging process itself might be a potential therapeutic target for the treatment and prevention of dementia. Numerous ce...
Article
Full-text available
Transcriptomic markers of aging can be useful for studying age‐related processes and diseases. However, noncoding repetitive element (RE) transcripts, which may play an important role in aging, are commonly overlooked in transcriptome studies—and their potential as a transcriptomic marker of aging has not been evaluated. Here, we used multiple RNA‐...
Article
Full-text available
One of the best strategies for healthy brain aging is regular aerobic exercise. Commonly studied "anti-aging" compounds may mimic some effects of exercise on the brain, but novel approaches that target energy-sensing pathways similar to exercise probably will be more effective in this context. We review evidence in support of this hypothesis by foc...
Article
Full-text available
Aging is the greatest risk factor most diseases, including cardiovascular disorders, cancers, diabetes, and neurodegeneration, but select nutritional interventions may profoundly reduce the risk for these conditions. These interventions include calorie restriction, intermittent fasting, protein restriction, and reducing intake of certain amino acid...
Article
Older age is the primary risk factor for most chronic diseases, including Alzheimer's disease (AD). Current preclinical models to study brain aging and AD are mainly transgenic and harbor mutations intended to mirror brain pathologies associated with human brain aging/AD (e.g., by increasing production of amyloid precursor protein, amyloid beta [Aβ...
Article
Aging is the primary risk factor for most chronic diseases, including Alzheimer's disease (AD). Current pre-clinical approaches for studying brain aging and AD rely primarily on transgenic models intended to mirror pathologies associated with human brain aging and neurodegeneration (e.g., by increasing production of amyloid beta (Aβ) and phosphoryl...
Article
Growing evidence shows that expression of non-coding repetitive sequences, or repetitive element (RE) transcripts, increases with age and may be related to biological aging itself. In fact, RE transcript levels can be used to accurately predict age, and they are reduced by healthy aging interventions. However, the exact mechanistic links between RE...
Article
Full-text available
Reduced protein intake, through dilution with carbohydrate, extends lifespan and improves mid-life metabolic health in animal models. However, with transition to industrialised food systems, reduced dietary protein is associated with poor health outcomes in humans. Here we systematically interrogate the impact of carbohydrate quality in diets with...
Article
Cancer is one of the most common age-related diseases, and over one-third of cancer patients will receive chemotherapy. One frequently reported side effect of chemotherapeutic agents like doxorubicin (Doxo) is impaired cognitive function, commonly known as “chemotherapy-induced cognitive impairment (CICI)”, which may mimic accelerated brain aging....
Chapter
Aging is the greatest risk factor for most neurodegenerative diseases including dementia. Nutrition has a profound impact on aging, and certain dietary interventions may have the potential to reduce the risk of neurodegenerative disease. Caloric restriction (CR) is one of the most robust nutritional interventions to improve brain health during agin...
Article
Full-text available
The Geometric Framework for Nutrition (GFN) has revealed that ad-libitum low-protein, high-carbohydrate (LPHC) diets improve cardiometabolic health and extend lifespan in rodents, but it is not known whether these diets are also beneficial for brain health. Here, we utilized previous results from GFN studies and compared hippocampus biology and mem...
Article
Full-text available
Background: Transcripts from non-coding repetitive elements (RE) in the genome may be involved in aging. However, they are often ignored in transcriptome studies on healthspan and lifespan, and their role in healthy aging interventions has not been characterized. Methods: We analyze RE in RNA-seq datasets from mice subjected to robust healthspan-...
Article
Full-text available
There is an unmet need and urgency to find safe and effective anti-obesity interventions. Our recent study in mice fed on obesogenic diet found that treatment with the alcohol aversive drug disulfiram reduced feeding efficiency and led to a decrease in body weight and an increase in energy expenditure. The intervention with disulfiram improved gluc...
Preprint
Full-text available
Transcripts from non-coding repetitive elements (RE) in the genome may be involved in aging. However, they are often ignored in transcriptome studies on healthspan and lifespan, and their role in healthy aging interventions has not been characterized. Here, we analyze RE in RNA-seq datasets from mice subjected to robust healthspan- and lifespan-inc...
Article
Obesity is a top public health concern, and a molecule that safely treats obesity is urgently needed. Disulfiram (known commercially as Antabuse), an FDA-approved treatment for chronic alcohol addiction, exhibits anti-inflammatory properties and helps protect against certain types of cancer. Here, we show that in mice disulfiram treatment prevented...
Article
Full-text available
Key points: Night time/active phase food restriction for 6 hr impaired glucose intolerance in young male and female mice. Females displayed increased capacity for lipogenesis and triglyceride storage in response to a short daily fast. Females had lower fasting insulin levels and an increased potential for utilizing fat for energy through β-oxidati...
Article
Full-text available
Elevated branched chain amino acids (BCAAs) are associated with obesity and insulin resistance. How long-term dietary BCAAs impact late-life health and lifespan is unknown. Here, we show that when dietary BCAAs are varied against a fixed, isocaloric macronutrient background, long-term exposure to high BCAA diets leads to hyperphagia, obesity and re...
Article
Full-text available
Background: Sirtuin 1 (SIRT1) is a NAD+-dependent enzyme that has important roles in many biological processes involved in aging, including cell growth and repair, inflammation, and energy regulation. SIRT1 activity is modulated in response to certain nutritional interventions that increase healthspan and longevity in rodents, including calorie re...
Article
The most robust interventions that impact on the biological processes of aging and age-related diseases are nutritional (caloric restriction, protein restriction, intermittent fasting) or those pharmacological and genetic interventions that act on nutrient sensing pathways. The best established nutritional intervention is caloric restriction, but t...
Article
Full-text available
Recent research has shown significant health benefits deriving from high-dietary fiber or microbiome-accessible carbohydrate consumption. Compared with native starch (NS), dietary resistant starch (RS) is a high microbiome-accessible carbohydrate that significantly alters the gut microbiome. The aim of this study was to determine the systemic metab...
Article
Full-text available
Calorie restriction (CR) increases lifespan and improves brain health in mice. Ad libitum low-protein, high-carbohydrate (LPHC) diets also extend lifespan, but it is not known whether they are beneficial for brain health. We compared hippocampus biology and memory in mice subjected to 20% CR or provided ad libitum access to one of three LPHC diets...
Article
Full-text available
Nutrition influences both hepatic function and aging, but mechanisms are poorly understood. Here, the effects of lifelong, ad libitum-fed diets varying in macronutrients and energy on hepatic gene expression were studied. Gene expression was measured using Affymetrix mouse arrays in livers of 46 mice aged 15 months fed one of 25 diets varying in pr...
Article
Full-text available
Resveratrol improves healthspan and lifespan in many organisms. Several different targets and mechanisms of action have been proposed to explain the beneficial effects of resveratrol on healthspan and longevity, including the activation of a family of proteins known as sirtuins and its action as a calorie restriction mimetic. In this mini-review, w...
Article
Full-text available
Macronutrients and dietary energy influence aging, age-related health and lifespan. Reduction in telomere length has been proposed as one mechanism for aging. Therefore this study investigated the effects of varying ratios of dietary macronutrients and energy on telomere length in older adult mice. C57Bl/6 mice were fed ad libitum their entire life...
Article
Full-text available
Evaluation of behavior and cognition in rodent models underpins mechanistic and interventional studies of brain aging and neurodegenerative diseases, especially dementia. Commonly used tests include Morris water maze, Barnes maze, object recognition, fear conditioning, radial arm water maze, and Y maze. Each of these tests reflects some aspects of...
Article
Fibroblast growth factor 21 (FGF21) is the first known endocrine signal activated by protein restriction. Although FGF21 is robustly elevated in low-protein environments, increased FGF21 is also seen in various other contexts such as fasting, overfeeding, ketogenic diets, and high-carbohydrate diets, leaving its nutritional context and physiologica...
Article
Full-text available
Calorie restriction (CR) is the most robust non-genetic intervention to delay aging. However, there are a number of emerging experimental variables that alter CR responses. We investigated the role of sex, strain, and level of CR on health and survival in mice. CR did not always correlate with lifespan extension, although it consistently improved h...
Article
Old age is the greatest risk factor for most neurodegenerative diseases. During recent decades there have been major advances in understanding the biology of aging, and the development of nutritional interventions that delay aging including calorie restriction (CR) and intermittent fasting (IF), and chemicals that influence pathways linking nutriti...
Article
Full-text available
Nutrition has profound effects on ageing and lifespan. Caloric restriction is the major nutritional intervention that historically has been shown to influence lifespan and/or healthspan in many animal models. Studies have suggested that a reduction in protein intake can also increase lifespan, albeit not as dramatically as caloric restriction. More...
Article
Full-text available
Previous studies have shown positive effects of long-term resveratrol (RSV) supplementation in preventing pancreatic beta cell dysfunction, arterial stiffening and metabolic decline induced by high-fat/high-sugar (HFS) diet in nonhuman primates. Here, the analysis was extended to examine whether RSV may reduce dietary stress toxicity in the cerebra...
Article
Full-text available
Although the basic morphological characteristics of neurons in the cerebellar cortex have been documented in several species, virtually nothing is known about the quantitative morphological characteristics of these neurons across different taxa. To that end, the present study investigated cerebellar neuronal morphology among eight different, large-...

Questions

Questions (6)
Question
I'm looking for software that can detect and count the number of live worms in a 90-second video. There are a few examples but none seem to work well or are outdated. Any advice is greatly appreciated.
Question
I was not sure if anyone could provide advice on this.  Microarray usually requires additional validation by qPCR. Does the same rule apply for RNA sequencing data?  What is the general consensus on this?  Any help is greatly appreciated.  Thank you. 
Question
I have been told not to wear headphones, even on low volume, during mouse behavioural work as the mice have an extremely keen sense of hearing and it can affect their behaviour.  As behavioural work is most certainly not the most exciting in science, I was curious if anyone has experience with this issue. Some people I know do wear headphones, but only noise cancelling, well built ones and on a very low volume.  They claim mice cannot hear the noise in this case. Others suggest no headphones at all and are extremely cautious.  If anyone can point me in the right direction that would be very helpful.  Thank you!
Question
I have whole brain lysates, and I would like to run a western blot and probe for Metallothionein (mW: 6KDa).  If anyone has success with this protein, please can you tell me what worked for you?  I have been having trouble with this because this protein is so small.  If anyone has some advice on transfer conditions, blocking conditions, etc., that would be great.  Any advice is greatly appreciated. Thank you. 
Question
I have been using a polytron, but perhaps there is a better way.
Question
I am attempting to measure brain inflammation in lysates from cortex tissue. Is there any test that is a good "overall" measure of brain inflammation from these lysates?

Projects

Projects (3)
Project
Repetitive element expression increases with chronological age and biologically older cells appear to express more repetitive elements than biologically younger.
Project
Understanding the mechanisms by which doxorubicin chemotherapy treatment contribute to premature aging phenotypes and neurological deficits could provide important insights for patient care and quality of life. This project seeks to characterize the transcriptomic changes cause by doxorubicin, and the nature of its effects on neurons, with the goal of finding potential therapies to rescue/prevent the toxic side effects.