Devendra Lingojwar

ADEETECHGENE BIOTECH PVT LTD · Diagnostic and Therapeutics: Human Virology and Human Genetics

Background: Sickle cell anemia (SCA) is an inherited monogenic disorder. The clinical symptoms of SCA are protean, including vaso-occlusion, hemolysis, early stroke, leg ulcers, multi-organ failure, and increased risk of premature death. Hematopoietic stem cell transplantation is the only treatment identified to reduce SCA-related organ damage. Unf...

Abstract Prevalence of different abnormal hemoglobins (Hb) in Indian tribal and nontribal population groups is well established. Sickle cell hemoglobin (HbS) is mostly concentrated in Central and South Indian states, whereas HbD and Hb E is mostly found in North-North-West and North-North-East states respectively. HbJ, an alpha globin gene variant,...

Around the world, more than 80% of the population utilizes traditional medicines for curing and managing diseases. In India, most of the Ayurvedic pharma companies uses different cultivars of Ayurvedic plants based on the presence of specific chemical contents, e.g. Eugenol content in Ocimum tenuiflorum Linn. Tulsi. These contents vary from cultiva...

Background Conjugation of Albumin (Alb) with six copies of PEG 5K chains using extension arm chemistry (EAF) generates a novel semisynthetic supra perfusion resuscitation fluid (EAF P5K6 Alb) that performs better than the conventional colloidal plasma expanders in extreme hemodilution and hemorrhagic shock animal models. The superior activity of E...

Introduction: Malaria and sickle cell disease (SCD) are more common in tribal populations of India, with varying prevalence amongst different ethnic groups. SCD affects mostly the socio-economically deprived communities, living in the three clusters of 10 neighboring states in India. Most of this endogamous population in India, including, scheduled...

Albumin surface decorated with multiple copies of PEG 5K (on an average six copies) using Extension Arm Chemistry (EAF-P5K6-Alb) and constituted to a viscosity comparable to that of conventional colloidal plasma expander like hetastarch or dextran 70 performs far superior in resuscitation of hemorrhagic shock models in terms of restoring the mean a...

Prevalence of different abnormal hemoglobins (Hb) in Indian tribal and nontribal population groups is well established. Sickle cell hemoglobin (HbS) is mostly concentrated in Central and South Indian states, whereas HbD and Hb E is mostly found in North-North-West and North-North-East states respectively. HbJ, an alpha globin gene variant, is earli...

Ocimum tenuiflorum Linn. Tulsi, is widely used in many Ayurvedic preparations, however, its molecular identification by fast and robust single amplicon marker is not properly understood. Most commonly studied molecular markers for plant identification from chloroplast, mitochondria and nuclear source, are not very easy for the rapid and efficient i...

The origin of haemoglobinopathies and prevalence of endemic malaria are inter-linked. Natural protection from malaria pathogenesis after infection might be the most likely and highly accepted hypothesis of origin of different alleles of normal adult haemoglobin in different population groups throughout the world, more specifically in the tropical c...

Azoreductase plays a key role in bioremediation and biotransformation of azo dyes. It initializes the reduction of azo bond in azo dye metabolism under aerobic or anaerobic conditions. In the present study, we isolated an alkaliphilic red-colored Aquiflexum sp. DL6 bacterial strain and identified by 16S rRNA method. We report nicotinamide adenine d...

Azo dyes and nitro-aromatic compounds are the largest group of pollutants released in the environment as industrial wastes. They create serious health and environmental problems. Azoreductases catalyze the reduction of azo dyes and nitro compounds to their respective amines. AN azoreductase was purified up to 12-fold from Lysinibacillus sphaericus...

The membrane-associated F1Fo-ATP synthase of bacteria plays a vital role in the production of energy molecule, i.e. adenosine triphosphate (ATP). However, under alkaline conditions, ATP synthesis in bacteria is not thermodynamically feasible due to external high pH. Various studies reported motifs in ATP synthase c-subunit as alkaliphile-specific f...

Nitroaromatic compounds are toxic to living organisms. Most of them exhibit human mutagenic and carcinogenic potential. Biotransformation and bioremediation processes can convert these compounds into non-toxic compounds. Acclimatization of bacterial strain Aquiflexum sp. DL6 with nitro-aromatics resulted in significant induction of nitroreductase (...

Lonar Lake, an Indian soda lake with high alkaline conditions of pH 10.5, is well known for its biodiversity of extremophiles including alkaliphiles. Most of the molecular studies on Lonar Lake alkaliphiles are based on identification by 16S ribosomal RNA (16S rRNA). Various studies have reported alkaliphiles from different alkaline habitats other...

Various studies reported motifs in ATP synthase c-subunit as alkaliphile specific features for adaptation in alkaline condition. Some conserved residues in alkaliphiles were observed in Stenotrophomonas species DL18 isolated from Indian alkaline Soda Lake, Lonar, which has pH 10.5. The above mentioned specific amino acid features in studied alkalip...

Indian Soda Lake, Lonar, has been studied because of its ancient status and significant bacterial diversity including alkaliphiles. However, these studies were inadequate to differentiate akaliphiles at molecular level and their ability as exoelectrogens. Present study deals with bacterial isolation, their identification based on 16S rRNA along wit...

Indian Soda Lake, Lonar, has been studied by different group of scientists and coworkers due to its significant bacterial diversity including alkaliphiles. However, these studies were inadequate to differentiate akaliphiles at molecular level and their ability as exoelectrogens. Present study deals with bacterial isolation from Lonar Lake and their...

Lonar Lake, an Indian Soda Lake, is well known for its biodiversity of extremophiles including alkaliphiles. Most of the molecular studies on Lonar Lake alkaliphiles are based on molecular identification by 16S ribosomal RNA along with numerous applications in the biotechnology industry. However, molecular basis of adaptation of these alkaliphiles...

Medicinal plants are valuable to sources of medicinal and many other pharmaceutical products. The plants used in the phyto-pharmaceutical preparations are obtained mainly from the natural growing areas. With the increase in the demand for the crude drugs, the plants are being over exploited. Intergenic spacer region 2 (ITS2) between 5.8S RNA and 28...

Intergenic spacer region 2 (ITS2) between 5.8S RNA and 28S RNA is well established genomic region for molecular identification of plant as potential universal barcode. The Present studies deals with molecular identification of Tinospora sinensis, by a unique ITS2 region. Briefly, the plant genomic DNA isolated by CTAB method and targeted for amplif...

Computer hardware may act as a reservoir for microorganisms and may contribute its transfer via user. This research seeks to investigate what kinds of microbes, especially aerobic bacteria that actually can contaminate computer hardware. Bacterial 16S rRNA genes generally contain nine “hypervariable regions” (V1 – V9) that demonstrate considerable...

On Apr 4, 2011 this sequence version replaced gi:317119844.

Isolation and molecular identification of catalytically active Bacillus lentus BI377 CH1 towards -N=N- cleavage. (P-120)