Desak Gede Budi Krisnamurti

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• Doctor of Philosophy
• Professor (Associate) at University of Indonesia

Background Indonesia is one of the countries that is endemic to avian influenza virus subtype H9N2. This study aims to compare the molecular characteristics of avian influenza virus (AIV) subtype H9N2 from West Java. Methods Specific pathogen-free (SPF) embryonated chicken eggs were used to inoculate samples. RNA extraction and RT–qPCR confirmed th...

Background Indonesia is one of the countries that is endemic to avian influenza virus subtype H9N2. This study aims to compare the molecular characteristics of avian influenza virus (AIV) subtype H9N2 from West Java. Methods Specific pathogen-free (SPF) embryonated chicken eggs were used to inoculate samples. RNA extraction and RT–qPCR confirmed t...

Introduction: The irrational use of medicines remains prevalent globally despite education efforts, leading to decreased treatment quality and increased healthcare costs. With the rise of online learning during the COVID-19 pandemic, virtual simulation offers a promising solution to enhance the teaching of rational medicine use among medical studen...

The avian influenza virus is an infectious agent that may cause global health problems in poultry and is potentially zoonotic. In the recent decades, bacterial-derived sialidases have been extensively studied for their ability to inhibit avian influenza virus infections. In this study, the antiviral activity of NanB sialidase from Pasteurella multo...

Background: The rapidly aging population and the growth of geriatric medicine in the field of internal medicine are not supported by sufficient gerontological training in many health care disciplines. There is rising awareness about the education and training needed to adequately prepare health care professionals to address the needs of the older...

Background Indonesia is one of the countries that is endemic to avian influenza virus subtype H9N2. This study aims to compare the molecular characteristics of avian influenza virus (AIV) subtype H9N2 from West Java. Methods Specific pathogen-free (SPF) embryonated chicken eggs were used to inoculate samples. RNA extraction and RT–qPCR confirmed t...

Objective This study aims to evaluate the effect of Clostridium perfringens sialidase treatment on monolayer cell behavior using computational screening and an in vitro approach to demonstrate interaction between enzyme-based drugs and ligands in host cells. Materials and Methods The in silico study was carried out by molecular docking analysis us...

Avian influenza virus are infectious agent that may cause global health problem issues in poultry and potentially zoonotic. The high risk of circulating antiviral agents due to genetic mutations is a concern, highlighting the need for development of new antiviral agents. In this study the antiviral activity of NanB sialidase from P. multocida was i...

Background Prediabetes is a condition of intermediate hyperglycemia that may progress to type 2 diabetes. Vitamin D deficiency has been frequently linked to insulin resistance and diabetes. The study aimed to investigate the role of D supplementation and its possible mechanism of action on insulin resistance in prediabetic rats. Method The study w...

Bacterial sialidase hydrolyzes sialic acid receptors, preventing viral infections. Previous study reported Clostridium perfringens sialidase has inhibited viral infection in cell culture and animal models. Since the receptors have not been independently demonstrated, the effects of sialidase on host cells and their behavior are still poorly known....

BACKGROUND The rapidly aging population and the growth of geriatric medicine in the field of internal medicine are not supported by sufficient gerontological training in many health care disciplines. There is rising awareness about the education and training needed to adequately prepare health care professionals to address the needs of the older ad...

Diabetes mellitus is a chronic disease that occurs because the pancreas does not produce enough insulin and the body cells cannot use the insulin effectively. Type 1 diabetes mellitus requires insulin therapy, whereas in type 2 diabetes mellitus insulin resistance occurs, so additional insulin therapy may or may not be necessary. Problems related t...

This experimental study was conducted to assess the general safety of Garcinia mangostana Linn microparticle extract on BALB/c mice renal function after 14 days of administration. In this experimental study, the microparticles of G. mangostana extract were wrapped in chitosan alginate capsules to reach the target organ effectively. BALB/c mice were...

Objective: The study aimed to determine the effect of Acalypha indica Linn. (AI) root extract and a combination of simvastatin-AI on improving the fatty pancreas in Sprague-Dawley rats induced with a high fructose and cholesterol diet. Materials and methods: Twenty-four male Sprague-Dawley rats were induced with a high fructose and cholesterol d...

Background and Aim: Chronic hyperglycemia in prediabetic individuals would progress to diabetes and lead to several systemic disruptions, including hematological parameters. This study aimed to investigate the correlation between prediabetes and hematological indices in a prediabetic rat model. Materials and Methods: Eighteen male rats were random...

BACKGROUND: The administration of high-fat and high-glucose in diet followed by a low-dose streptozotocin injection in rats could mimic hyperglycemia, prediabetic, or diabetic conditions in humans. However, whether the rat model may lead to early liver impairment was still unclear. AIM: This study was aimed to investigate the possible changes in li...

The aim of this study is to investigate the activity which maintain the engagement of students and improve student's learning achievement in the herbal medicine module during a distance learning course that was conducted by the faculty of 62 Krisnamurti, Kekalih, Fachri, and Rahadiani medicine Universitas Indonesia through Student Centered e-Learni...

Introduction Newly graduated medical doctors in their internships are positioned to strengthen the front line in combating COVID-19. We developed a Massive Open Online Course (MOOC) to equip them with adequate knowledge for COVID-19 management. This paper aims to analyze the MOOC and evaluate participant satisfaction and increase in knowledge after...

Non-communicable diseases, such as cardiovascular diseases or kidney dysfunctions, tend to have expensive and unavailable treatments. This fact, coupled with the rising trend of such diseases, means that priority should be given towards preventive measures. One such measure is the administration of antioxidants to counter the increasing inflammator...

Aging is a time-related process leading to progressive deterioration of physiological bodily functions and increased vulnerability to degenerative disorders. The current trend of rapid growth in the global aging population poses a challenge for healthcare due to the increasing incidence of chronic diseases. In spite of this, preventive anti-aging a...

Aging is physiological process characterized by degeneration of body functions and increased oxidative stress level. Malondialdehyde (MDA) is an oxidative stress marker which concentration will rise along with age. Increased level of MDA is associated with tissue aging and degeneration. In other hands, herbal plants are often used as an alternative...

Chitosan-alginate coated microparticle of Garcinia mangostana Linn extract promises an alternative treatment of colon cancer patients, as the component of Garcinia mangostana Linn extract has anticancer activity in colon cancer cell lines, and chitosan-alginate could deliver compound faster to colon and accelerate its absorption. However, there hav...

Diet with high fructose and cholesterol (HFHC) plays a role in the increasing incidence of diabetes mellitus and metabolic syndrome. Ethanol extract from Acalypha indica has been known to alleviate hyperglycemia and hyperuricemic conditions in rats induced by substances that destroy beta cells. This research aimed to evaluate its effect in rats ind...

Chitosan-alginate coated microparticle of Garcinia mangostana Linn extract promises an alternative treatment of colon cancer patients, as the component of Garcinia mangostana Linn extract has anticancer activity in colon cancer cell lines, and chitosan-alginate could deliver compound faster to colon and accelerate its absorption. However, there hav...

Diet high in fructose and cholesterol may lead to the development of diabetic nephropathy (DN). One of the first drugs of choice in DN treatment is captopril. Prolonged use of this drug may lead to some adverse effects and the treatment can be optimized through using other therapy options. Acalypha indica Linn. (AI) may be an alternative herbal the...

Objective: Tamoxifen is the drug of choice to treat breast cancer positive for estrogen receptor. Long-term use of tamoxifen can induce multidrug resistance (MDR) associated with decreased sensitivity of cancer cells to the drug. One of the causes of MDR is overexpression of efflux transporter multidrug resistance-associated protein (MRP)2. Various...

Objective: The need for long-term medication in diabetes mellitus has led to a search for herbal medicines as alternative treatments. Several studies have shown that extract or isolates of Garcinia mangostana can help prevent and treat type 2 diabetes mellitus (T2DM). Methods: This review was conducted by searching various databases, including PubM...

Aging is an inevitable time-related process leading to reduced cognition and physical strength also increased susceptibility of degenerative disorders. This study studies the aging process related to relatively high oxidative stress level and proinflammatory mediators such as interleukin-6 (IL-6). Centella Asiatica (CA), a traditional medicinal her...

Aging is a condition of impaired function due to the decrease of physiological integrity that can be a risk factor for another significant disease. The aging process is caused by free radicals that can damage the surrounding molecules. This process can cause oxidative stress and increase the level of proinflammatory mediators, such as interleukin-6...

Type 2 diabetes mellitus is a metabolic disorder that is characterized by hyperglycemia, insulin resistance, inadequate insulin secretion, and excessive glucagon secretion. Metformin is the first-line medication prescribed for type 2 diabetes mellitus. Prolonged use of metformin can cause mild and serious side effects. One of the alternative herbal...

High fructose and cholesterol diets (HFCDs) cause hypercholesterolemia, hypertriglyceridemia, and many acute and chronic serious diseases. Current established treatments, such as simvastatin (SIM) and gemfibrozil (GEM), have been successful in lowering cholesterol and triglyceride levels, but their long-term use poses a risk for organ dysfunction....

strong>Background: Approximately 80–85% of strokes are ischemic and lead to alterations in neuronal cell morphology and cell death. There is a lack of studies on the effect of the combination of Acalypha indica L. (AI) and Centella asiatica L. (CA) in terms of its neurotherapy property. This study was conducted to investigate the neurotherapeutic e...

Objective: Myasthenia gravis (MG) is an autoimmune disease of the neuromuscular junction (NMJ) caused by antibodies that attack components of the postsynaptic membrane, impair neuromuscular transmission, and lead to weakness and fatigue of skeletal muscle. Acetylcholine is also used as a neurotransmitter in the autonomic nervous system. Striated ca...

Objective: Myasthenia gravis (MG) is an autoimmune disease of the neuromuscular junction (NMJ) caused by antibodies that attack components of the postsynaptic membrane, impair neuromuscular transmission, and lead to weakness and fatigue of skeletal muscle. Acetylcholine is also used as a neurotransmitter in the autonomic nervous system. Striated ca...

Functional decrease in learning and memory is one of the characteristics of the aging process. It has beenknown that a lower concentration of Brain Derived Neurotrophin Factor (BDNF) found on the brain, playsa role in the phenomenon. This study was designed to determine whether a herbal plant, Centella asiatica(CA), would increase the BDNF level on...

Hypercholesterolemia is a common metabolic disorder found worldwide, which is associated with dyslipidemia and cardiovascular disease incidence. Among the first line choices drugs is gemfibrozil but it has side effect gastrointestinal disorder. The aim of this study is to investigate anti hypercholesterolemia effect of ethanol extract of Acalypha i...

Tamoxifen is the first line drug used in the treatment of estrogen receptor-positive (ER+) breast cancer. The development of multidrug resistance (MDR) to tamoxifen remains a major challenge in the treatment of cancer. One of the mechanisms related to MDR is decrease of drug influx via overexpression of drug efflux transporters such as P-glycoprote...

Improved isolation and effective purification of the main polar lipid (main glycophospholipid, MPL) from Thermoplasma acidophilum DSM 1728/10217 was achieved by a three-step procedure including (i) extraction of total lipid from lyophilized cells and removal of hydrophilic contaminants, (ii) fractionation of the total lipid via diethylaminoethyl (D...