Dennis Bazylinski

Dennis Bazylinski
University of Nevada, Las Vegas | UNLV · School of Life Sciences

About

372
Publications
63,461
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15,651
Citations
Citations since 2017
1 Research Item
4736 Citations
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20172018201920202021202220230200400600800
20172018201920202021202220230200400600800

Publications

Publications (372)
Article
Full-text available
We report here the genome sequence of Magnetovibrio blakemorei MV-1, a marine vibrioid magnetotactic bacterium with a single polar flagellum. The current assembly consists of 91 contigs with a combined size of 3,638,804 bp (54.3% G+C content). This genome allows for further investigations of the molecular biomineralization mechanisms of magnetosome...
Article
Full-text available
Significance Magnetotactic bacteria are one of the simplest systems that perform biomineralization: organisms that create inorganic materials using biochemistry under genetic control. They synthesize magnetosomes, which are intracellular, membrane-bound nanoscale single crystals of magnetite, a magnetic iron oxide. We studied the magnetism of indiv...
Article
Although dissimilatory sulfate-reducing bacteria (SRB) are generally described as strictly anaerobic organisms with regard to growth, several reports have shown that some SRB, particularly Desulfovibrio species, are quite resistant to O2. For example, SRB remain viable in many aerobic environments while some even reduce O2 to H2O. However, reproduc...
Article
Full-text available
Importance: Magnetotaxis is a navigational mechanism used by magnetotactic bacteria to move along geomagnetic field lines and find an optimal position in chemically stratified sediments. For that, magnetotactic bacteria swim parallel to the geomagnetic field lines under oxic conditions in the Northern Hemisphere, whereas those in the Southern Hemi...
Article
Magnetotactic bacteria (MTB) are a heterogeneous group of ubiquitous aquatic microorganisms capable of biomineralizing nano-sized, membrane-bound, magnetic iron-rich mineral particles called magnetosomes. MTB are found in chemically-stratified aquatic sediments and/or water columns with a wide range of salinities, moderate to high temperatures, and...
Poster
Full-text available
Cosmopolitan in their distribution, magnetotactic bacteria (MTB) are motile, aquatic, microaerophilic-to-anaerobic prokaryotes that passively align along geomagnetic field lines and represent a morphologically, physiologically and phylogenetically diverse assemblage of organisms. MTB are characterized by their ability to biomineralize magnetosomes,...
Article
Full-text available
Magnetotactic bacteria mineralize nanometer-size crystals of magnetite (Fe3O4) through a series of protein-mediated reactions that occur inside of organelles called magnetosomes. Mms6 is a transmembrane protein thought to play a key role in magnetite mineralization. We used both electron and fluorescent microscopy to examine the subcellular locatio...
Article
Full-text available
div class="title">Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging - Volume 21 Issue S3 - Taylor J. Woehl, Sanjay Kashyap, Emre Firlar, Teresa Perez-Gonzalez, Damien Faivre, Denis Trubitsyn, Dennis A. Bazylinski, Tanya Prozorov
Article
The measurements of magnetic hysteresis for aggregates of nanoparticles deposited on a surface are reported. Magnetite nanoparticles derived from magnetotactic bacteria are studied using nanomechanical torque magnetometry. The nanoparticles are deposited on high-stress Si3N4 membranes, to allow inspection by electron microscopy, followed by focused...
Article
Magnetotactic bacteria are a diverse group of prokaryotes that share the unique ability of biomineralizing magnetosomes, which are intracellular, membrane-bounded crystals of either magnetite (Fe3O4) or greigite (Fe3S4). Magnetosome biomineralization is mediated by a number of specific proteins, many of which are localized in the magnetosome membra...
Article
Full-text available
Magnetotactic bacteria (MTB) produce intracellular organelles called magnetosomes which are magnetic nanoparticles composed of magnetite (Fe3O4) or greigite (Fe3S4) enveloped by a lipid bilayer. The synthesis of a magnetosome is through a genetically controlled process in which the bacterium has control over the composition, direction of crystal gr...
Article
Full-text available
Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures i...
Article
Magnetotactic bacteria (MTB) are unique in their ability to synthesize intracellular nano-sized minerals of magnetite and/or greigite magnetosomes for magnetic orientation. Thus, they provide an excellent model system to investigate mechanisms of biomineralization. MTB play important roles in bulk sedimentary magnetism, and have numerous versatile...
Article
Full-text available
The biomineralization of magnetotactic bacterial magnetite nanoparticles is a topic of intense research due to the particles' narrow size distribution and magnetic properties. Incorporation of foreign metal ions into the crystal matrix of magnetotactic bacterial magnetite has been previously examined by a number of investigators. In this study, cel...
Article
Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures i...
Article
Full-text available
Microorganisms living in gradient environments affect large-scale processes, including the cycling of elements such as carbon, nitrogen or sulfur, the rates and fate of primary production, and the generation of climatically active gases. Aerotaxis is a common adaptation in organisms living in the oxygen gradients of stratified environments. Magneto...
Article
Full-text available
Desulfonatronum thiodismutans strain MLF1, an alkaliphilic bacterium capable of sulfate reduction, was isolated from Mono Lake, California. Here we report the 3.92-Mb draft genome sequence comprising 34 contigs and some results of its automated annotation. These data will improve our knowledge of mechanisms by which bacteria withstand extreme envir...
Article
Full-text available
Magnetotactic bacteria are a diverse group of prokaryotes that biomineralize intracellular magnetosomes, composed of magnetic (Fe3O4) crystals each enveloped by a lipid bilayer membrane that contains proteins not found in other parts of the cell. Although partial roles of some of these magnetosome proteins have been determined, the roles of most ha...
Conference Paper
Full-text available
Magnetotactic bacteria (MTB) are a paraphyletic group of prokaryotes distinguished by their ability to mineralize nanocrystalline magnetite (Fe 3 O 4), greigite (Fe 3 S 4), and/or pyrite (FeS 2) within intracellular, pseudo-organelles known as magnetosomes. This form of biomineralization is tightly regulated at the genetic level with the cell contr...
Article
Magnetotactic bacteria are motile, mostly aquatic, ubiquitous prokaryotes whose direction of swimming is profoundly influenced by the Earth's and other magnetic fields. These microorganisms biomineralize magnetosomes which are intracellular, tens of nanometer sized, membrane-bounded magnetic crystals of the minerals magnetite (Fe3O4) and greigite (...
Article
Full-text available
Although magnetotactic bacteria (MTB) are ubiquitous in aquatic habitats, they are still considered fastidious microorganisms with regard to growth and cultivation with only a relatively low number of axenic cultures available to date. Here, we report the first axenic culture of an MTB isolated in the Southern Hemisphere (Itaipu Lagoon in Rio de Ja...
Article
Full-text available
Pavilion Lake is a slightly alkaline, freshwater lake located in British Columbia, Canada (50°51'N, 121°44'W). It is known for unusual organosedimentary structures, called microbialites that are found along the lake basin. These deposits are complex associations of fossilized microbial communities and detrital- or chemical-sedimentary rocks. During...
Article
Full-text available
Magnetotactic bacteria (MTB) biomineralize magnetosomes, nano-scale crystals of magnetite or greigite in membrane enclosures that comprise a permanent magnetic dipole in each cell. MTB control the mineral composition, habit, size, and crystallographic orientation of the magnetosomes, as well as their arrangement within the cell. Studies involving m...
Article
Candidatus Magnetoglobus multicellularis (Ca. M. multicellularis) is a member of a group of uncultured magnetotactic prokaryotes that possesses a unique multicellular morphology. To better understand this organism's physiology, we used a genomic approach through pyrosequencing. Genomic data analysis corroborates previous structural studies and reve...
Article
Full-text available
SUMMARY Magnetotactic bacteria (MTB) are widespread, motile, diverse prokaryotes that biomineralize a unique organelle called the magnetosome. Magnetosomes consist of a nano-sized crystal of a magnetic iron mineral that is enveloped by a lipid bilayer membrane. In cells of almost all MTB, magnetosomes are organized as a well-ordered chain. The magn...
Article
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of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.
Chapter
Full-text available
Magnetotactic bacteria represent a diverse group of motile prokaryotes that biomineralize intracellular, membrane-bounded, and tens-of-nanometer-sized crystals of a magnetic mineral, either magnetite (Fe3O4) or greigite (Fe3S4). These structures are called magnetosomes and cause cells to align along the Earth’s geomagnetic field lines as they swim,...
Article
Magnetotactic bacteria (MTB) represent a group of diverse motile prokaryotes that biomineralize magnetosomes, the organelles responsible for magnetotaxis. Magnetosomes consist of intracellular, membrane-bounded, tens-of-nanometre-sized crystals of the magnetic minerals magnetite (Fe3 O4 ) or greigite (Fe3 S4 ) and are usually organized as a chain w...
Article
Full-text available
The bacterial magnetosome is a unique prokaryotic organelle comprising magnetic mineral crystals surrounded by a phospholipid bilayer. These inclusions are biomineralized by the magnetotactic bacteria which are ubiquitous, aquatic, motile microorganisms. Magnetosomes cause cells of magnetotactic bacteria to passively align and swim along the Earth'...
Article
Full-text available
Magnetotactic bacteria (MTB) represent a diverse collection of motile prokaryotes that biomineralize intracellular, membrane-bounded, tens-of-nanometer-sized crystals of a magnetic mineral called magnetosomes. Magnetosome minerals consist of either magnetite (Fe3O4) or greigite (Fe3S4) and cause cells to align along the Earth’s geomagnetic field li...
Article
Full-text available
The growth and magnetosome production of the marine magnetotactic vibrio Magnetovibrio blakemorei strain MV-1 were optimized through a statistics-based experimental factorial design. In the optimized growth medium, maximum magnetite yields of 64.3 mg/liter in batch cultures and 26 mg/liter in a bioreactor were obtained.
Article
Horizontal gene transfer (HGT), the transfer of genetic material other than by descent, is thought to have played significant roles in the evolution and distribution of genes in prokaryotes. These include those responsible for the ability of motile, aquatic magnetotactic bacteria (MTB) to align and swim along magnetic field lines and the biomineral...
Article
Full-text available
There is a good deal of published evidence that indicates that all magnetosomes within a single cell of a magnetotactic bacterium are magnetically oriented in the same direction so that they form a single magnetic dipole believed to assist navigation of the cell to optimal environments for their growth and survival. Some cells of the cultured magne...
Chapter
Full-text available
Prokaryotes that exhibit magnetotaxis, collectively known as the magnetotactic bacteria, are those whose direction of motility is influenced by the Earth’s geomagnetic and externally applied magnetic fields. These ubiquitous, aquatic microorganisms represent a morphologically, phylogenetically, and physiologically diverse group that biomineralize u...
Chapter
This book presents the green synthesis of nanoparticles by algae, diatoms, bacteria and plants, and discusses the mechanisms behind the synthesis of nanoparticles. The book should be immensely useful for students, researchers and teachers of biology, chemistry, chemical technology, nanotechnology, microbial technology and those who are interested i...
Article
The magnetic properties (first-order reversal curves, ferromagnetic resonance and decomposition of saturation remanent magnetization acquisition) of Magnetovibrio blakemorei, a cultivated marine magnetotactic bacterium, differ from those of other magnetotactic species from sediments deposited in lakes and marine habitats previously studied. This fi...
Article
Full-text available
A magnetotactic bacterium, designated strain MV-1T, was isolated from sulfide-rich sediments in a salt marsh near Boston, Massachusetts. Cells of strain MV-1T are Gram-negative, and vibrioid to helicoid in morphology. Cells are motile by means of single polar flagellum. Cells appear to display a transitional state between axial and polar magnetotax...
Data
##Assembly-Data-START## Assembly Method :: SABIA v. System for Automated Bacterial Integrated Annotation Sequencing Technology :: 454 ##Assembly-Data-END##
Data
##Assembly-Data-START## Assembly Method :: SABIA v. System for Automated Bacterial Integrated Annotation Sequencing Technology :: 454 ##Assembly-Data-END##
Data
##Assembly-Data-START## Assembly Method :: SABIA v. System for Automated Bacterial Integrated Annotation Sequencing Technology :: 454 ##Assembly-Data-END##
Data
##Assembly-Data-START## Assembly Method :: SABIA v. System for Automated Bacterial Integrated Annotation Sequencing Technology :: 454 ##Assembly-Data-END##
Data
##Assembly-Data-START## Assembly Method :: SABIA v. System for Automated Bacterial Integrated Annotation Sequencing Technology :: 454 ##Assembly-Data-END##
Data
##Assembly-Data-START## Assembly Method :: SABIA v. System for Automated Bacterial Integrated Annotation Sequencing Technology :: 454 ##Assembly-Data-END##
Article
Full-text available
Vibrioid- to helical-shaped magnetotactic bacteria phylogenetically related to the genus Magnetospirillum were isolated in axenic cultures from a number of freshwater and brackish environments located in the southwestern United States. Based on 16S rRNA gene sequences, most of the new isolates represent new Magnetospirillum species or new strains o...
Article
Full-text available
A strictly anaerobic Gram-positive non-spore-forming bacterium designated as Lachnospiraceae bacterium ACC2 strain DSM 24645T was isolated from the human subgingival dental plaque. Bacterial cells were 4-40 µm long non-motile rods often swollen and forming curved filaments up to 200 µm. Cells contained intracellular, poorly crystalline, nanometer-s...
Article
It has never been demonstrated whether magnetite synthesized through the heat-dependent decomposition of carbonate precursors retains the chemical and structural features of the carbonates. In this study, synthetic (Ca,Mg,Fe)CO3 was thermally decomposed by heating from 25 to 700 °C under 1 atm CO2, and by in situ exposure under vacuum to the electr...
Article
Full-text available
Magnetotactic bacteria are a morphologically, metabolically and phylogenetically disparate array of bacteria united by the ability to biomineralize membrane-encased, single-magnetic-domain mineral crystals (magnetosomes) that cause the cell to orient along the Earth's geomagnetic field lines. The most commonly observed type of magnetotactic bacteri...
Article
Scanning transmission X-ray microscopy (STXM) measurements at the C K, O K and Fe L3 edges were used to study the magnetotactic bacterium Candidatus Magnetovibrio blakemorei strain MV-1 on an individual cell basis. Improved data acquisition methodologies resulting in higher quality results are presented. Visualization of magnetosomes from their O K...
Chapter
Full-text available
Magnetotactic protists of different types have been sporadically observed in a number of aquatic habitats, mainly at the oxic-anoxic transition zone of chemically stratified coastal environments and the anoxic zone. Cells of those examined contained magnetite (Fe3O4) crystals whose size and shape are close to those of magnetotactic bacteria. While...
Article
Full-text available
Magnetotactic bacteria contain magnetosomes--intracellular, membrane-bounded, magnetic nanocrystals of magnetite (Fe(3)O(4)) or greigite (Fe(3)S(4))--that cause the bacteria to swim along geomagnetic field lines. We isolated a greigite-producing magnetotactic bacterium from a brackish spring in Death Valley National Park, California, USA, strain BW...
Chapter
Two novel magnetotactic bacteria (MTB) were isolated from sediment and water collected from the Badwater Basin, Death Valley National Park and southeastern shore of the Salton Sea, respectively, and were designated as strains BW-2 and SS-5, respectively. Both organisms are rod-shaped, biomineralize magnetite, and are motile by means of flagella. Th...
Chapter
Full-text available
Bacterial inclusions can be defined as discrete structures seen within the confines of prokaryotic cells, generally intracytoplasmic, but in some instances in the periplasmic region of the cell. Inclusions function as metabolic reserves, cell positioners, or as metabolic organelles. Some inclusions may contribute to more than one of these functions...
Article
Full-text available
A marine, magnetotactic bacterium, designated strain MMS-1(T), was isolated from mud and water from a salt marsh in Woods Hole, Massachusetts, USA, after enrichment in defined oxygen-concentration/redox-gradient medium. Strain MMS-1(T) is an obligate microaerophile capable of chemoorganoheterotrophic and chemolithoautotrophic growth. Optimal growth...