Denisse Tatiana Molina Aulestia

Denisse Tatiana Molina Aulestia
Federal University of Paraná | UFPR · Departamento de Engenharia de Bioprocessos e Biotecnologia

Doctor of Engineering

About

24
Publications
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378
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Introduction
Skills and Expertise

Publications

Publications (24)
Chapter
Algae, recognized for their nutritional value and versatility, are increasingly integrated into various food products, reflecting growing consumer awareness and demand for healthier, environmentally friendly options. Seaweed species, renowned for their high protein content1 and beneficial amino acid composition, have long been valued as significant...
Chapter
The advent of next-generation sequencing (NGS) technologies represented a watershed moment in microbiological research, opening up the possibility to study sub-dominant groups, late-growing species, and previously uncultivable microorganisms within the fermentation process. The first NGS platform developed was the Roche 454. Since then, DNA sequenc...
Chapter
Industrial production of microalgae requires large volumes of water. Agro-industry wastewaters are generated in large volumes and must be adequately treated. The connection of both processes is only natural—conventional wastewater treatment processes end with a stabilizing pond that has a self-established microbiota, which includes microalgae. This...
Article
Microalgae-mediated industrial flue gas biofixation has been widely discussed as a clean alternative for greenhouse gas mitigation. Through photosynthetic processes, microalgae can fix carbon dioxide (CO2) and other compounds and can also be exploited to obtain high value-added products in a circular economy. One of the major limitations of this bi...
Chapter
Waste management and environmental impacts remain fields that require assessment alongside the advances in cultivated meat production. Water and energy consumption, the demand for natural resources, and the generation of residues throughout the production chain must all be considered. These aspects can be more effectively adjusted in the early stag...
Article
Full-text available
Lutein, a yellow xanthophyll carotenoid, is increasingly recognized for its nutraceutical benefits, particularly in protecting the retina’s macula from age-related degeneration. Microalgae are a promising source of lutein, which can be a primary product or a coproduct in biorefineries. Certain microalgae exhibit lutein levels (up to 1.7%) surpassin...
Chapter
The growing demand for bioproducts from algae is being met by constant improvement in mass cultivation and downstream. However, there still is a gap between the perceived possibilities of microalgal products and the reality of feasible and profitable production. In order to reach economic production of microalgae bioproducts such as biofuels, nutri...
Article
Biological contamination is one of the main bottlenecks in microalgae production, reducing quality and productivity and sometimes leading to the complete loss of the cultures. Selecting terpenes can be a pathway toward eco-friendly contamination control in microalgae cultures. This work evaluated the presence of bacterial contaminants in N. oleoabu...
Article
Full-text available
Biological contamination is one of the main bottlenecks in microalgae production, reducing quality and productivity and sometimes leading to the complete loss of the cultures. Selecting terpenes can be a pathway toward eco-friendly contamination control in microalgae cultures. This work evaluated the presence of bacterial contaminants in N. oleoabu...
Preprint
Full-text available
Biological contamination is one of the main bottlenecks in microalgae production, reducing quality and productivity and sometimes leading to the complete loss of the cultures. Selecting terpenes can be a pathway toward eco-friendly contamination control in microalgae cultures. This work evaluated the presence of bacterial contaminants in N. oleoabu...
Article
Full-text available
Recycling bioresources is the only way to sustainably meet a growing world population’s food and energy needs. One of the ways to do so is by using agro-industry wastewater to cultivate microalgae. While the industrial production of microalgae requires large volumes of water, existing agro-industry processes generate large volumes of wastewater wit...
Preprint
Full-text available
Biological contamination is one of the main bottlenecks in the production of microalgae, reducing quality and productivity, sometimes leading to complete loss of the cultures. This work evaluated the presence of bacterial contaminants in N. oleoabundans cultures through HTS and 16S analysis and their susceptibility to 6 natural terpenes (α-pinene,...
Article
Full-text available
The antioxidant and food pigment astaxanthin (AX) can be produced by several microorganisms, in auto- or heterotrophic conditions. Regardless of the organism, AX concentrations in culture media are low, typically about 10–40 mg/L. Therefore, large amounts of nutrients and water are necessary to prepare culture media. Using low-cost substrates such...
Chapter
Microbial lipids can be used as food, feed, and nutraceuticals products. Their recovery from fermentation broths or microalgal cultivation requires dewatering and further extraction, followed by purification using processes similar to those applied for other fats and oils. The initial downstream steps are critical for the economical production of b...
Chapter
The areal productivity and lipid yield of algal cultures created an expectation for algal biofuels, which is yet to be materialized; the projected prices for algal liquid fuels make them still uncompetitive. However, the market for microalgae products is growing in size and diversity, primarily for nutritional biomasses such as Spirulina or Chlorel...
Chapter
Lipids from microbial sources are valuable substitutes of animal and vegetable oils, with potential applications such as food ingredients and supplements, biofuels, biolubricants, biosurfactants, and wax esters. They gained prominence as partial substitutes of fish-oil in specialty food products. Eicosapentaenoic acid, docosahexaenoic acid (omega-3...
Article
Full-text available
Cassava processing wastewater (CPW) is a highly polluting, liquid residue of cassava processing, usually discarded or treated anaerobically. However, it can serve as a low-cost culture medium for microalgae. After a preliminary evaluation of the growth of 10 microalgal strains in diluted CPW, the microalgae Haematococcus pluvialis SAG 34−1b and Neo...
Article
Full-text available
Microalgae are emerging sources for sustainable production of bioproducts and bioenergy. However, a problem with its reliable production is the biological contamination of cultures. There are several alternatives to mitigate this problem, such as using axenic cultures, concentrated inocula, or applying chemical control agents, which should be eco-f...
Article
Bio-based plastics represent only 1% of world production, while 98% are plastics of fossil origin. This shows how far we are from an ecologically correct scenario we are, and how much more needs to be done to develop bioplastic processes. Biopolymer production from monomers generated by fermentation, such as some organic acids, is a sustainable alt...
Article
The use of effluents for hydrogen production through dark fermentation is promising because it results in the generation of value-added products and reduction of the effluent's organic load. A low-cost medium using agroindustrial effluents, corn steep liquor (CSL) and cassava processing wastewater (CPW) was evaluated for hydrogen production with mi...
Article
Microalgae from several ecosystems in the state of Paraná, Brazil, near the Atlantic Forest, were isolated using selected culture media. From 30 isolates, 20 with moderate growth were evaluated for its suitability as industrial strains. A multicriteria analysis was used considering process and composition dimensions: 1) kinetic parameters versus bi...
Article
Microalgae are sources of nutritional products and biofuels. However, their economical processing is challenging, because of (i) the inherently low concentration of biomass in algal cultures, below 0.5%, (ii) the high-water content in the harvested biomass, above 70%; and (iii) the variable intracellular content and composition. Cell wall structure...
Article
Full-text available
Microalgae are versatile sources of bioproducts, a solution for many environmental problems. However, and despite its importance, one of the main problems in large-scale cultures—the presence of contaminants—is rarely systematically approached. Contamination, or the presence of undesirable organisms in a culture, is deleterious for the culture and...

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