Denise Maria Zezell

• Ph.D. in Physics
• Professor (Full) at Instituto de Pesquisas Energéticas e Nucleares IPEN/CNEN

Background: According to the WHO, oral cancer is the thirteenth most common cancer worldwide, with tobacco use being one of the primary causes of oral cancer. This study aimed to characterize and differentiate the saliva and bound water using FTIR spectroscopy in smoking and non-smoking individuals. Materials and Methods: This prospective observati...

To evaluate the efficacy and safety of different wavelengths of high-power diode lasers for the treatment of dentin hypersensitivity by analyzing morphological changes and temperature variation. Human third molars were irradiated with five different commercially available lasers at wavelengths of 808 nm, 940 nm, 976 nm, and 980 nm, both with and wi...

This study was done to evaluate whether the irradiation of dentine with ultrashort laser pulses prior to adhesive procedures contributes to increase the adhesive resistance to microtraction essays. Twenty-four human teeth (third molars) were used, divided into 4 groups (n=6): standard adhesive procedures with etch-rinse adhesive were used in the co...

Background/Objectives: Beta-lactam antibiotics, derived from penicillin, are the most used class of antimicrobials used for treating bacterial infections. Over the years, microorganisms have developed resistance mechanisms capable of preventing the effect of these drugs. This condition has been a significant public health concern for the 21st centu...

Objective To evaluate the efficacy and safety of different wavelengths of high-power diode lasers for the treatment of dentin hypersensitivity by analyzing morphological changes and temperature variation. Methods Human third molars were irradiated with four different wavelengths from five commercial brands. Temperature variation was measured using...

Antibiotic monitoring is an essential aspect of patient care, including monitoring the levels of these drugs in the bloodstream. This practice aims to ensure the best possible treatment, minimizing adverse effects and the risk of antibiotic resistance. In addition to drug monitoring, rapid and accurate detection of antibiotics plays a crucial role...

The aim of this study was to verify the effectiveness of attenuated total reflectance‐fourier transform infrared (ATR‐FTIR) spectroscopy in the characterization of bone repair in mandibular osteotomy using erbium, chromium‐doped yttrium, scandium, gallium and garnet (Er,Cr:YSGG) laser and multilaminate drill on each side. Two mandible bone fragment...

Thyroid Cancer is a disease in which abnormal cells grow uncontrollably in the gland with the potential to invade other organs. Every year, almost 44,000 new cases are diagnosed worldwide. Histopathological diagnosis of fine-needle aspiration biopsies of thyroid nodules is the most precise exam to confirm the diagnosis and estimate the stages of th...

Changes in the crystal lattices and compositions of the dental enamel powders were evaluated after oven or Er,Cr:YSGG laser irradiation. A decrease in the Ca/P ratio to levels close to the ideal theoretical value of laser irradiation caused a considerable decrease in the hydroxyapatite crystallite size but without changes in the crystallographic ph...

Tropical diseases present significant challenges to global health, particularly in resource-limited regions. Early and accurate detection of these diseases is vital for effective management and control. In recent years, metallic-based LSPR sensors have emerged as promising diagnostic tools for sensitive and rapid detection of tropical diseases. Thi...

Diabetes mellitus (DM) is a widespread and rapidly growing disease, and it is estimated that it will impact up to 693 million adults by 2045. To cope this challenge, the innovative advances in non-destructive progressive urine glucose-monitoring platforms are important for improving diabetes surveillance technologies. In this study, we aim to bette...

This in vitro study aimed to evaluate the preventive action of topical fluoride application combined with laser irradiation under different pH conditions using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). A total of 180 samples of human dental enamel were prepared and divided into groups: Negative Control,...

Here, we apply a 3D discriminant analysis approach to analyze FTIR hyperspectral images of normal vs malignant Melanoma (MM) samples for skin cancer diagnosis. For this porpose we used 2 samples, for Normal (49k) and for MM(90k). Our results evidence the outstanding performance with accuracy up to 81% for big data (> 100k).

The 2022 Latin America Optics and Photonics Conference (LAOP 2022), the major international conference sponsored by Optica in Latin America, returned to Recife, Pernambuco, Brazil, after its first edition in 2010. Held every two years since (except for 2020), LAOP has the explicit objective to promote Latin American excellence in optics and photoni...

Breast cancer (BC) molecular subtypes diagnosis involves improving clinical uptake by Fourier transform infrared (FTIR) spectroscopic imaging, which is a non-destructive and powerful technique, enabling label free extraction of biochemical information towards prognostic stratification and evaluation of cell functionality. However, methods of measur...

This work shows the importance of data preprocessing steps before applying the k-means clustering method in hyperspectral images of oral squamous cell carcinoma samples to differentiate healthy tissue from neoplastic tissue in the samples.

Photobiomodulation (PBM) has been clinically used for the prevention and treatment of oral mucositis (OM). The effect of red and near-infrared wavelengths on OM repair is still misunderstood. The aim of this study was to compare the clinical effect and tissue changes caused by 660 nm and 780 nm exposure in an experimental model of OM. Rats were sub...

The mechanisms of action of photobiomodulation (PBM) in oral mucositis (OM) are not completely elucidated. To enlighten the role of PBM in the evolution of epithelial maturity in OM ulcers, the present study evaluated the effect of PBM with red (λ) wavelength of 660 nanometers (nm) and infrared of 780 nm in radio-induced OM wounds on the tongue of...

The development of novel platforms for non-invasive continuous glucose monitoring applied in the screening and monitoring of diabetes is crucial to improve diabetes surveillance systems. Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy of urine can be an alternative as a sustainable, label-free, fast, non-invasive, and...

Objectives: The primary aim of this ex vivo study was to evaluate thermal damage and cutting efficiency of micro and super pulsed diode lasers. The secondary aim was to suggest a guideline to perform simple surgical excisions adequate for histopathological evaluation. Material and methods: Ten groups of 10 specimens of pig tongues were excised u...

Objectives Compare saliva between smoking and nonsmoking patients by Fourier transform infrared (FT-IR) spectroscopy as an optical biopsy technique and associate with data processing methods based on artificial intelligence. It will be possible to investigate biochemical changes at the molecular level and in the future use it for early diagnosis of...

This study aimed to evaluate the effect of desensitizing and conventional mouth rinses on dentin tubule occlusion. Dentin hypersensitivity was simulated by EDTA application for five minutes. The specimens were randomly allocated into the following groups: desensitizing mouth rinses (Colgate Sensitive, Elmex Sensitive Professional, Listerine Advance...

The localized surface plasmon resonance (LSPR) due to light–particle interaction and its dependence on the surrounding medium have been widely manipulated for sensing applications. The sensing efficiency is governed by the refractive index-based sensitivity (ηRIS) and the full width half maximum (FWHM) of the LSPR spectra. Thereby, a sensor with hi...

Background: The debonding of crowns and ceramic veneers with laser is already a reality in the clinic. It presentsbenefits in comparison to traditional removal with the use of a rotating instrument; however, there is still noconsolidated protocol and many professionals use it without the necessary scientific basis. Objective: The aim ofthis work wa...

Triple-negative breast cancer (TNBC) overexpresses the Epidermal Growth Factor Receptor (EGFR), a characteristic of different types of tumors, linked to worse disease prognosis and risk of recurrence. Conventional treatments are aggressive and, on several occasions, have a poor prognosis, which may be related to the clinical heterogeneity of tumors...

There is already a societal awareness of the growing impact of nanoscience and nanotechnology, with nanomaterials (with at least one dimension less than 100 nm) now incorporated in items as diverse as mobile phones, clothes or dentifrices. In the healthcare area, nanoparticles of biocompatible materials have already been used for cancer treatment o...

The health care application of ionizing radiation has expanded worldwide during the last several decades. While the health impacts of ionizing radiation improved patient care, inaccurate handling of radiation technology is more prone to potential health risks. Therefore, the present study characterizes the bone dose response using bovine femurs fro...

Objective: The purpose of this short communication is to show that although photobiomodulation in the treatment of neurophathic oral pain after COVID-19 contagion could be an option, photobiomodulation is not a new technique. PBMT was used with different protocols and pain was assessed using VAS (visual analogue scale - 0 until 10) before and after...

High-intensity lasers are widely used in dental procedures and the heating produced on the surface is necessary to ensure protective activity against the development of caries and erosion lesions. However, caution should be exercised regarding the spread of heat to the pulp, periodontal tissue and alveolar bone, which can cause harm to these tissue...

The objective of the present work is to show the relationship of the high-wavenumber spectral region concern OH vibrations, which show in a way how bound water can be altered in different sample groups.

FTIR spectroscopy imaging in addition to deep learning is a potential tool for breast cancer subtype classification, where accuracies higher than 86% can be achieved to predict among all subtypes.

FTIR spectroscopy was able to discriminate bone samples receiving low-dose ionizing radiation doses (0.002 kGy, 0.004 kGy, 0.007 kGy), offering valuable insights in view of the understanding of radiation dose response in biological systems.

We present a computational framework based on machine learning classifiers K-Nearest Neighbors and Neighborhood Component analysis for breast cancer (BC) subtypes prognostic. Our results has up to 97% accuracy for prognostic stratification of BC subtypes.

Here, we employ segmentation and convolutional neural network (CNN) to identify and quantify enamel demineralization. Our results depict that CNN model using input SEM images achieve accuracy up to 79% for enamel demineralization diagnosis.

The aim of this study was to evaluate surface wear, presence of microcracks and surface irregularities of WaveOne (WO) and WaveOne Gold (WOG) instruments before and after multiple uses. Eight Primary instruments of the WO and WOG systems were evaluated, each one was used to prepare six mesial canals of extracted human mandibular molars. The surface...

The breast cancer molecular subtype is an important classification to outline the prognostic. Gold-standard assessing using immunohistochemistry adds subjectivity due to interlaboratory and interobserver variations. In order to increase the diagnosis confidence, other techniques need to be examined, where the FTIR spectroscopy imaging allied with m...

ProposeEffective strategies to control the development of dental erosion are still needed. This study evaluated the effect of associating a modified Er,Cr:YSGG laser protocol with topical fluoride application on dentin erosion.Methods Sound and eroded dentin specimens (n = 10/substrate) were allocated into groups: control (no treatment); APF gel (1...

Los tumores primarios de la piel, el carcinoma de células basales (BCC), el carcinoma de células escamosas (SCC) y el melanoma, representan un tercio del total de las neoplasias en el ser humano y están en ín ma relación con la exposición al UV. La espectrofotometría IR resulta un método auxiliar de diagnós co, que cons tuye una técnica no invasiva...

To identify characteristics to differentiate normal skin and malignant cutaneous tumors by IFTR.

Objective This in vitro study characterized and monitored, by Optical Coherence Tomography (OCT) and Fourier Transformed Infrared Spectroscopy (FTIR), the effects of the association of acidulated phosphate fluoride gel (APF-gel) and Nd:YAG (neodymiun:yttrium-aluminum-garnet) laser, as sequencial treatments, in the prevention of incipient enamel car...

Since patients suffer pain in the post-surgery of bone repair interventions, bioactive glass/hydrogel drug delivery systems containing local anesthetics, such as ropivacaine, could improve patient life quality by combining bone regeneration with anesthetics. However, poloxamer-based hydrogel properties are sensitive to ions, temperature, and water...

Objective To evaluate the efficacy of Nd:YAG laser associated with calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion after erosive/abrasive challenges. Methodology Dentin specimens were exposed to 17% ethylene diamine tetra-acetic acid (EDTA) solution for 5 min and randomly allocated into five groups: G1, control (...

Objectives This study aimed to investigate the influence of Nd:YAG laser and aluminum oxide sandblasting on the shear bond strength (SBS) of lingual brackets and to optically analyze the behavior of the enamel morphology.Materials and methodsThirty-five bovines’ incisors teeth were divided into 5 groups (n = 7), according to the surface preconditio...

Thyroglobulin is a glycoiodoprotein that is produced by thyroid follicular cells; it is stored in follicles in structures known as colloids. The main function of this protein is to stock the hormones triiodothyronine (T3) and thyroxine (T4) until the body requires them. This study aims to demonstrate that infrared spectral imaging with appropriate...

Femtosecond pulses from a Ti:Sapphire laser were used to irradiate specimens of yttria-stabilised (35% mol) tetragonal zirconia (Y-TZP) with the purpose of studying the effects of the irradiations on their surface properties and morphology after ageing. Zirconia disks were divided into eight groups (n = 32) according to their surface treatment and...

Laminate veneer removal is becoming a routine procedure at the dental clinic and the use of laser can facilitate its removal. This work aimed to evaluate the morphological, elemental, and optical changes in the remaining enamel after veneer removal using Er,Cr:YSGG laser. Forty‐four enamel slabs were prepared and randomly distributed into nine expe...

Burns are one of the leading causes of morbidity worldwide and the most costly traumatic injuries. A better understanding of the molecular mechanisms in wound healing is required to accelerate tissue recovery and reduce the health economic impact. However, the standard techniques used to evaluate the biological events associated to wound repair are...

Aim: To compare the effect of erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser at different irradiation parameters and acid etching on the shear bond strength (SBS) of orthodontic brackets to enamel. Materials and methods: Forty bovine incisors were randomly distributed into groups (n = 10): GI: 37% phosphoric acid etching; GI...

Fourier Transform Infrared (FTIR) spectroscopy is a label-free analytical technique used to evaluate the chemical profile of a sample based on its molecular vibrations. The potential dermatological applications of FTIR spectroscopy has been well demonstrated over the past decades through many proof-of-concept studies evaluating cancerous and non-ca...

We present the proof of concept of a general model that uses the tissue sample transmittance as input to estimate the depth‐resolved attenuation coefficient of tissue samples using Optical Coherence Tomography. This method allows us to obtain an image of tissue optical properties instead of intensity contrast, guiding diagnosis and tissues differen...

The present study evaluated the protective effects that are induced by fluoride and Nd:YAG laser irradiation to control dentine erosion. One hundred and twenty bovine dentine slabs were eroded with citric acid twice a day for a total period of 15 days. At day 10, the samples were randomly assigned into eight groups: C (Control); F (Fluoride); L1 (N...

Objective: To evaluate the effect of Er,Cr:YSGG laser associated or not with acidulated phosphate fluoride (APF) on the control of enamel erosion progression. Design: Enamel slabs (4 mm × 4 mm × 2 mm) from bovine incisors were flattened, polished, and received a tape on their test surfaces, leaving a 4 mm × 1 mm area exposed. Specimens were erod...

The pursuit of perfection makes younger people undergo aesthetic procedures without formal indication. However, young patients may be susceptible to a disease such as head and neck cancer which treatment can compromise the adhesion of these indirect mate- rials. Here, we present an analyze, of the gamma radiation effects on crystallographic morphol...

Objective To evaluate dentin permeability and tubule occlusion of in-office desensitizing treatments, and to analyze their resistance to erosive/abrasive challenges. Design Ninety-one 1mm-thick dentin discs were immersed in EDTA solution for 5 min. After analyzing the maximum dentin permeability, the specimens were randomly allocated into 7 expe...

Purpose: The purpose of this study was to evaluate the effects of four in-office desensitizing products on dentin tubule occlusion and erosive wear. Methods: Dentin hypersensitivity was simulated by EDTA application for five minutes. The specimens were randomly allocated into five groups (n=11), according to treatment: No treatment - Control (C)...

Alterations in the amide (1500–1700 cm⁻¹) spectral region probed by Fourier-transform infrared spectroscopy (FTIR) have been reported comparing tumor and normal tissues. Usually, bands in this range are assigned to the so-called Amide I, II, and III vibrations which provide pieces of information concerning peptide bonds and secondary structure (α-h...

Objective and background: The correct selections of the cementing agent, the endodontic post material and placement protocol are critical to provide an increased longevity of the teeth that went through endodontic treatment. The irradiation with diode laser before post cementation, can promote an antimicrobial effect. However, there is a lack of i...