Deni Rahmat

Deni Rahmat
Pancasila University, Jakarta, Indonesia · Pharmaceutical Technology

Professor

About

81
Publications
25,353
Reads
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1,076
Citations
Additional affiliations
March 2013 - June 2013
University of Innsbruck
Position
  • PostDoc Position
Description
  • Synthesis of Cyclodextrin-SH and HPMC-SH and SNEDDS Formulation

Publications

Publications (81)
Article
Aim: The aim of this study was to investigate the mucus-penetrating properties of neutral nanoparticles comprising poly(acrylic acid) (PAA) and poly(allylamine) (PAM). Materials & methods: PAA and PAM nanoparticles were prepared on the basis of ionic interactions between the two polymers. Nanoparticles were characterized by particle size as well...
Article
The aim of this study was to design thiolated surface stabilized superparamagnetic iron oxide nanoparticles (TSS-SPIONs) for efficient internalization with high MRI sensitivity. TSS-SPIONs were developed by chelation between thiolated chitosan-thioglycolic acid (chitosan-TGA) hydrogel and iron ions (Fe2+/ Fe3+). Likely, unmodified chitosan hydrogel...
Article
The purpose of this study was to design and characterize a novel cationic thiolated polymer. In this regard a hydroxyethylcellulose-cysteamine conjugate (HEC-cysteamine) was synthesized. Oxidative ring opening with periodate and reductive amination with cysteamine were performed in order to immobilize free thiol groups to HEC. The resulting HEC-cys...
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Secondary metabolites content in Sida rhombifolia L. (SR) are believed to inhibit the xanthine oxidase (XO) enzyme, which is responsible for converting hypoxanthine and xanthine into uric acid. This study aimed to compare flavonoid levels, XO inhibitory activity, and the reduction of uric acid levels of sidaguri herb, stem, leaves, and root extract...
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Objective: Collagen is a fibrous protein that confers strength and flexibility on bone and other body tissues, such as the skin and tendons, and is the main component of the extracellular matrix. It is used in cosmetics for its anti-aging activity. The primary source of commercial collagen is pig skin, but its use is imperfect due to halal concerns...
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Objective: Dahlia tuber (Dahlia sp.) is one of the inulin sources that could be planted in Indonesia. Inulin is fructan-based polysaccharide. Therefore, the research aimed to investigate inulin from the extract of dahlia tuber as a drug excipient, especially for nanoparticles based on inulin-cysteamine thiomer. Methods: Crude inulin was isolated fr...
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Objective: Binahong leaves (Anredera cordifolia (Ten.) Steenis) is an herbal plant that is most commonly used to cure various types of diseases in Indonesia, including diabetes. The objective of this study was to conduct a comparative analysis of the antidiabetic activities between binahong leaves extract and binahong leaves extract nanoparticles....
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The Indonesian people empirically use Sidaguri (Sida rhombifolia L.) in medicine, such as anti-hyperuricemia. Herbal-based treatment is currently much preferred. Drug metabolism, in general, mainly occurs in the liver, so the possibility of damage to this organ is high. This study aims to determine the acute toxicity category of 96% ethanol extract...
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Objective(s) Skin aging is a degenerative process that can be induced by UV irradiation. UV radiation can produce reactive oxidate stress which causes premature aging. This study aims to examine the antiaging potential of secretome gel (SC) from human Wharton Jelly Mesenchymal Stem Cells (hWJ-MSCs) in a UVB-induced mice model. Materials and Method...
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Kaempferia galanga Linn is a typical Indonesian plant that contains the compound Ethyl P-Methoxy Sinnamate which has antibacterial activity. Acne is a common skin condition characterized by inflammation of the hair follicles and oil glands in the skin. The most effective acne treatment is the use of antibiotics. This research is an experimental stu...
Article
Eye shadow is one of the decorative cosmetic that contains color that is applied to the eyelids. Eye shadows were generally blue, pink, dark red, silver, green, and brown. Natural dyes are derived from plants, animals, and microorganisms. Butterfly pea flower (Clitoria ternatea L.) and secang wood (Caesalpinia sappan L.) are plants that contain nat...
Article
Clitoria ternatea flowers contain blue anthocyanin that has potential as natural coloring. The purpose of this study was to obtain an eyeliner product containing natural coloring that is safe, stable, and can replace hazardous chemicals. Clitoria ternatea flowers were extracted by kinetic maceration with 70% ethanol. The obtained extract was subjec...
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Black Sea cucumbers (Holothuria atra) are a potential source of active metabolites such as flavonoids widely used for nutraceutical, pharmaceutical, and cosmeceutical products. The type of nanoparticle that is often used in cosmetics and skin products is Solid Lipid Nanoparticles (SLN). In this study, the SLN of black sea cucumber extract will be p...
Article
Rimpang Zingiber montanum memiliki potensi sebagai antioksidan dan tabir surya. Pelarut etanol merupakan pelarut yang diijinkan dalam pembuatan ekstrak untuk bahan obat tradisional. Etanol 70% dan 96% lazim digunakan dalam proses ekstraksi namun belum diketahui bagaimana pengaruh kedua konsentrasi etanol terhadap kandungan senyawa aktif dan aktivit...
Article
Nanoteknologi telah merevolusi berbagai bidang, termasuk kedokteran, dengan memberikan peluang baru untuk penelitian dan aplikasi. Penelitian ini berfokus pada karakterisasi nanopartikel yang berasal dari ekstrak daun sirsak. Nanopartikel disintesis menggunakan polimer kitosan dan ekstrak daun sirsak, dan sifat-sifatnya dianalisis. Hasil penelitian...
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Luka diabetes akan mengalami fase inflamasi yang lebih lama dibandingkan luka normal, terjadinya gangguan peredaran darah besar dan kecil mengakibatkan sirkulasi darah menjadi kurang optimal, pemberian nutrisi dan oksigenasi berkurang sehingga proses penyembuhan luka diabetes menjadi lebih lama. Penelitian ini bertujuan untuk melihat aktivitas gel...
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This study aims to characterize the microencapsulated type of human Adipose Tissue Mesenchymal Stem Cells (hAT-MSCs) and nanoparticles containing a conditioned medium of hAT-MSCs (CM-hATMSCs). The hAT-MSCs microencapsulation was measured the cells viability on 1st, 7th, and 14th days, by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethylphenyl)-2-(4-s...
Article
Several studies revealed that Syzygium polyanthum (Wight) Walp, known as bay leaves, has the potential to be used as herbal medicine due to its active compounds including flavonoids. Herbal medicines are chosen to replace synthetic drugs which have side effects on health. However, some active substances of herbal medicines including bay leaves are...
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Beluntas leaf (Pluchea indica L. Less) and red ginger rhizome (Zingiber officinale Roscoe) is a plan have activity as antibacterial causes acne. Beluntas leaf contain antibacterial compounds from alkaloid, flavonoid free type flavonol, steroid and tannin. Red ginger rhizome extract compounds flavonoid, essential oil, polifenol, and saponin. This st...
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Advantages of Cantigi (Vaccinium varingiaefolium Miq.) include anti-inflammatory, Spasmolytic, Antiviral, and Hypotensive Properties. The objective of this study was to standardize the Cantigi leaf extract. Non-specific parameters include total ash content, acid insoluble ash content, water content, dry loss, determination of heavy metals (Hg, As,...
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Okra (Abelmoschus esculentus L.) merupakan salah satu tanaman yang digunakan sebagai sayuran. Bagian tanaman yang sering digunakan adalah buah. Buah okra mengandung flavonoid yang berpotensi sebagai bahan alam yang berkhasiat sebagai obat. Penelitian tentang skrining fitokimia dan penetapan kadar flavonoid total dari ekstrak buah okra (Abelmoschus...
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Objective: The aims of this study were to formulate and characterize the nanoparticles containing conditioned medium adipose tissue mesenchymal stem cell (CM-ATMSC). Methods: Adipose Tissue Mesenchymal Stem Cell (AT-MSC) was cultured with supplemented Modified Eagle Medium (MEM) Alpha in an incubator with 37 °C and 5% CO2. The conditioned medium wa...
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Objective: The purpose of this study was the determination of the quality parameters of the antidiabetic activity of 70% ethanol extract and Kembang Bulan leaf nanoparticle extract. Methods: Kembang Bulan powder was macerated kinetic with 70% ethanol, evaporated until a thick extract was obtained using a rotary evaporator, then specific and non-spe...
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Objective: WJMSC is a stem cell-derived from Wharton's Jelly the umbilical cord of pregnant women which has the ability to differentiate into other cells that are osetogenic, myogenic, neurogenic, and hematopoietic. Stem cell microencapsulation is a cell coating technique that is expected to act as a delivery vehicle. This study aims to make stem c...
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Purpose Ethyl cellulose (EC) based nanoparticles are being extensively studied for their ability to achieve prolonged drug release and improve drug stability. Within this study, the thiolation of unmodified EC using cysteamine as a ligand was carried out to design nanoparticles with mucoadhesive properties and comparatively strong lipophilic proper...
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Background: Propionibacterium acnes is a commensal bacteria that play a role in developing acne vulgaris on the skin. Antibacterial activity assay in the last decades have shown that Propionibacterium acnes is resistant to several antibiotics. Potential zingiber genera such as Z. officinale, Z. montanum, and Z. zerumbet have potential as antibacter...
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Objective: This research aimed to prepare curcumin microcapsules by the spray drying method and to evaluate their characteristics. Methods: The microcapsules were prepared by the spray drying method. The generated microcapsules were evaluated for organoleptic, morphology, particle size, the percentage of curcumin and water content. Furthermore, the...
Article
Tetrahidrokurkumin (THC) adalah salah satu senyawa polifenol yang memiliki khasiat sebagai pencerah kulit. THC merupakan turunan kurkumin dimana THC memiliki warna serbuk kuning muda hingga putih. Penelitian ini bertujuan untuk mengetahui potensi aktivitas penghambatan enzim tirosinase terbaik dari senyawa tetrahidrokurkumin yang diformulasi dalam...
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Dyslipidemia is a condition caused by disruption of lipid metabolism due to the interaction of genetic factors and environmental factors. There is strong evidence of an association between LDL cholesterol and cardiovascular events based on clinical outcome studies, so LDL cholesterol is the main target in the management of dyslipidemia. In addition...
Article
Temulawak (Curcuma xanthorrhiza Roxb.) is a medicinal plant for the raw material of traditional medicine in Indonesia. Temulawak can be a source of antioxidants and natural sunscreen because of its curcumin content. This study was conducted to determine antioxidant and sunscreen activity on Curcuma rhizome extract and nanoparticles extract and maki...
Article
Kosmetik digunakan untuk berbagai tujuan misalnya untuk membuat penampilan menarik. Dari sekian banyak jenis kosmetik yang digunakan, krim pencerah kulit banyak diminati terutama oleh para wanita di Asia, termasuk Indonesia, agar diperoleh tampilan kulit wajah yang putih dan bersih. Tujuan dari penelitian ini adalah untuk mengembangkan formulasi se...
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Limbah bulu ayam yang mengandung protein keratin telah direkomendasikan sebagai sumber nutrisi alternatif yang dapat digunakan untuk pakan ternak, industri kosmetik, dan industri pupuk. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh konsentrasi NaOH terhadap kualitas hidrolisat bulu ayam. Penelitian dilakukan dengan optimasi proses hid...
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Cisplatin has been used for decades for the treatment of ovarian cancer. However, despite its potent anticancer effect, cisplatin’s efficacy as a single agent was inadequate in patients with advanced stage. Curcumin has been shown to sensitize cisplatin activity in several cancer models. However, the low bioavailability of curcumin has limited its...
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Daun beluntas (Pluchea indica L. Less) dan rimpang jahe merah (Zinngiber officinalle Roscoe) merupakan tanaman yang mempunyai aktivitas sebagai antibakteri penyebab jerawat. Daun beluntas mengandung senyawa anti bakteri yang berasal dari alkaloid, flavonoid bebas jenis flavonol, steroid dan tanin. Ekstrak rimpang jahe merah mengandung flavonoid, mi...
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Purpose: This study aimed to provide the method of preparation, characterization of curcumin-loaded chitosan-sodium tripolyphosphate (NaTPP) nanoparticle, and evaluate its pharmacokinetic profiles. Methods: Curcumin-loaded chitosan-NaTPP nanoparticles were synthesized using ionic gelation methods. Curcumin was dissolved using surfactants and cosur...
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Fukosantin merupakan pigmen dari golongan karetenoid yang terdapat pada rumput laut coklat. Fukosantin memiliki sifat sebagai antioksidan, karena fukosantin mudah terdegredasi oleh pH, suhu dan cahaya. Untuk melindungi fukosantin dari degradasi tersebut maka dibuat menjadi sediaan nanospare. Penelitian ini bertujuan mendapakan senyawa fukosantin ya...
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Objective: Okra (Abelmoschus esculentus (L.) Moench) has potential antidiabetic activity. This study created a nanoemulsion of okra extract (NOE) and examined its activity on alloxan-induced diabetes mellitus in mice. Methods: Okra was macerated with 70% ethanol and dried in a rotary evaporator into the crude extract. The extract was encapsulated i...
Article
Minyak biji nyamplung dilaporkan memiliki kandungan vit E 2,05% dan β karoten sebesar 8,44mg/100g, minyak kelapa sawit dilaporkan memiliki kandungan vit E 27,6mg/100g dan β karoten 505mg/kg sehingga dapat berfungsi sebagai antioksidan dan tabir surya. Tujuan penelitian ini adalah mengetahui peningkatan atau penurunan nilai antioksidan dan SPF dari...
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Objective: The aim of this study was to investigate the antidiabetic activity of Javanese turmeric rhizome extract and its nanoparticles. Javanese turmeric rhizome contains curcumin, flavonoid, and xanthorrhizol that show the antidiabetic activity. The plant extracts entrapped within the nanoparticles have been reported to increase the active compo...
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Objective: The aim of the present study was to formulate bromelain into nanoparticles in order to improve its stability and activity. Methods: Crude bromelain was prepared by protein precipitation from the pineapple stem juice using ammonium sulphate at the concentration of 60% (w/v). Nanoparticles containing crude bromelain were generated using th...
Article
Minyak wijen dan minyak biji bunga matahari yang mengandung Vitamin E dan β-Karoten dilaporkan memiliki aktivitas antioksidan dan tabir surya. Penelitian aktivitas tabir surya pada minyak wijen memberi hasil nilai SPF sebesar 1,77. Tujuan penelitian ini adalah untuk mendapatkan kombinasi minyak wijen dan minyak biji bunga matahari yang memiliki akt...
Article
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Hidroksi Propil Selulosa (HPC)-Sisteamin merupakan tiomer kationik hasil modifikasi HPC yang dikonjugasikan dengan gugus sulfidhril dari sisteamin. Tujuan dari penelitian ini adalah untuk membuat nanopartikel berbasis tiomer HPC-Sisteamin dengan sifat mukoadhesif yang baik. Pembuatan nanopartikel berbasis HPC-Sisteamin dilakukan dengan metode gelas...
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Temulawak mempunyai berbagai aktivitas biologi, diantaranya, antikanker, antiinflamsi dan antibakteri. Tujuan penelitian ini adalah memformulasi sediaan gel yang mengandung nanopartikel ekstrak rimpang temulawak sebagai antiacne. Gel yang terbentuk dievaluasi secara fisik meliputi: organoleptik, homogenitas, viskositas dan sifat alir, kemampuan men...
Article
Background: Cisplatin is a first-line chemotherapeutic agent for various solid tumors including ovarian and breast cancer. Thereby, it has been proven effective as an antineoplastic agent, but its clinically use is limited because of its nephrotoxicity side effect. Aims and objectives: This study aimed to investigate curcumin as a renoprotector...
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Treatment for ovarian carcinoma is still far from optimal, animal models are still needed to study human epithelial ovarian cancer. Animal models of ovarian cancer are very important for understanding the pathogenesis of the disease and for testing new treatment strategies. Ovarian carcinogenesis models in mice have been modified and repaired to pr...
Article
Latar Belakang: Rumput laut cokelat (Sargassum.sp) salah satu biota laut yang senyawanya telah dipublikasi mempunyai khasiat sebagai antiaterosklerosis, namun data toksisitasnya perlu juga dieksplorasi keamanannya. Tujuan: Peneltian ini bertujuan untuk mendapatkan senyawa aktif dari sargassum sp. dan toksisitasnya secara in-silico. Metode:Penapisan...
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Indonesia has various beneficial plant species which have not been cultivated well yet. Blimbi (Averrhoa bilimbi L.) is among those plants which have antibacterial activities against Staphylococcus aureus and Escherichia coli. As carriers, the nanoparticles of this plant dissolve, trap, encapsulate, and attach the chemical preparation inside its ma...
Article
Telah dilakukan penelitian mengenai formulasi granul instan dari ekstrak etanol daun tahongai (Kleinhovia hospita L.) sebagai Antioksidan. Granul instan dibuat dengan menggunakan metode granulasi basah dengan variasi konsentrasi PVP FI (2,5%), FII (3%), FIII (3,5%). Granul instan dilakukan evaluasi dan uji aktivitas antioksidan dengan menggunakan m...
Article
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Objective: Thiomers have been known as polymer with mucoadhesive properties. The aim of this study was to synthesize the mucoadhesive potential of hydroxypropyl cellulose-cysteamine conjugate (HPC-cysteamine).Methods: The parent polymer HPC was chemically modified by introducing sulphydryl bearing compound using reductive amination. HPC-cysteamine...
Article
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Objective: The aim of this study was to compare the antibacterial activity of 96% (v/v) ethanolic extract of Javanese turmeric rhizome (Curcuma xanthorrhiza Roxb.) and nanoparticles containing the extract against 4 pathogenic bacteria.Methods: The synthesis of chitosan nanoparticles used ionic gelation method. The nanoparticles were evaluated for p...
Article
Javanese turmeric rhizome (Curcuma xanthorrhiza Roxb.) has been known to have many health benefits as antibacterial, anti-inflammatory, antidiabetic and anticancer. The purpose of this study was to compare the anti-inflammatory activity of temulawak rhizome extract with its nanoparticles. Temulawak rhizome extract was obatained by maceration using...
Article
Until now, needs of collagen are fulfilled mostly from beef or poultry. As there are more diseases found in poultry, the source of collagen from the skin and scales of fish becomes one promising alternative. The observed collagen parameters include proximate analysis, SDS-Page and heavy metals. To strengthen penetration of collagen, the application...
Article
The exposure to excessive and long lasting sun can cause adverse effects on the skin of erythema and even skin cancer. Jatropha curcas Linn is one of the plants that have potential substants to protect skin from the adverse effects of UV rays. Sunscreen preparations derived from ethyl acetate fractions of Jatropha leaves are made. Ethyl acetate fra...
Article
Pineapple stem contains bromelain enzyme with highest concentration compared to the other parts. The aim of the research was to formulate an anti dandruff creambath containing nanoparticles of pineapple stem which was physically and chemically stable and could inhibit the growth of Pityrosporum ovale. The pineapple stem extract was then formulated...
Conference Paper
Full-text available
Latar belakang: Pelepasan obat dengan sistem mukoadhesif membutuhkan suatu polimer yang memiliki sifat mukoadhesif. Kitosan adalah salah satu polimer dengan sifat mukoadhesif. Tiomer kitosan sistein merupakan hasil modifikasi kimia dari kitosan dan sistein yang memiliki gugus tiol sehingga dapat meningkatkan karakteristik mukoadhesif. Suatu nanosis...
Article
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Daun Sirsak (Annona muricata Linn), Annonaceae telah diketahui memiliki kemampuan meredam radikal bebas yang kuat dan bisa dikembangkan menjadi sediaan farmasi antioksidan. Pembentukan nanopartikel dari ekstrak akan meningkatkan stabilitas, kemampuan mukoadhesif dan penetrasi ekstrak sehingga dapat meningkatkan efektifitasnya. Pembuatan nanopartike...
Article
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Daun Pulai (Alstonia scholaris (L). R. BR) merupakan salah satu jenis tumbuhan yang telah diketahui berkhasiat sebagai antidiabetes. Ekstraksi daun pulai dilakukan secara maserasi kinetik dengan etanol 70% dan dipekatkan sehingga didapatkan ekstrak kental. Ekstrak kental diubah menjadi nanopartikel dengan metode gelasi ionik. Nanopartikel ekstrak k...
Article
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Objective: Test the antioxidant activity of banana peel extract, watermelon rind extract, and combinations, also in lotion dosage form.Methods: The antioxidant activity of each extract and their combinations were tested with 2,2-diphenyl-1-picrylhydrazil method and formulated intoa lotion dosage forms. Evaluation of the lotion dosage forms, includi...
Article
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Bonggol nanas (Ananas comosus (L.). Merr) mengandung enzim bromelain dengan kadar tertinggi dibandingkan bagian nanas lainnya. Salah satu fungsi enzim bromelain adalah sebagai antimikroba. Ekstrak bonggol nanas dibuat dalam bentuk nanopartikel dengan menggunakan kitosan kemudian dikeringkan dengan metode freeze drying. Serbuk kering ekstrak ditentu...
Conference Paper
Full-text available
Angiotensin I converting enzyme (ACE) has an important role in increase blood pressure thus the usage of compounds that inhibit ACE activity can lower the symptom of hypertension. Pletekan (Ruellia tuberosa L.) leaves are empirically used as antihypertension. It was therefore, the aims of this study to characterize pharmacognosy and phytochemistry,...
Article
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Within this study, HEC-cysteamine nanoparticles with free thiol groups in the range of 117-1548 μmol/g were designed and characterized. Nanoparticles were generated via ionic gelation of the cationic polymer with tripolyphosphate (TPP) followed by covalent crosslinking via disulfide bond formation using H(2)O(2) as oxidant. The mean diameter of the...
Article
The objective of this study was to evaluate the biodegradability of thiolated chitosans in comparison to unmodified chitosan. Mediated by carbodiimide, thioglycolic acid (TGA) and mercaptonicotinic acid (MNA) were covalently attached to chitosan via formation an amide bond. Applying two different concentrations of carbodiimide 50 and 100 mM, two ch...
Article
Chitosan (CTS) and chitosan-thioglycolic acid (CTS-TGA) nanoparticles loaded with four aqueous extracts of Thai anti-cancer medicinal plants were prepared by ionic interaction with tripolyphosphate (TPP). The averages sizes, zeta-potentials and PDI of the CTS and CTS-TGA nanoparticles loaded with the aqueous extracts were in range of 309.09±66.3 to...
Article
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The study was aimed to prepare a series of poly(acrylic acid)-cysteine-2-mercaptonicotinic acid conjugates (preactivated thiomers) and to evaluate the influence of molecular mass or degree of preactivation with 2-mercaptonicotinic acid (2MNA) on their permeation enhancing properties. Preactivated thiomers with different molecular mass and different...
Article
Full-text available
Within this study, the influence of particle size and zeta potential of hydroxyethyl cellulose-cysteamine particles on permeation enhancing properties was investigated. Particles were prepared by four different methods namely ionic gelation, spray drying, air jet milling and grinding. Particles prepared by grinding were additionally air jet milled....
Article
The objective of this study was the investigation of permeation enhancing and P-glycoprotein (P-gp) inhibition effects of a novel thiolated chitosan, the so-named S-protected thiolated chitosan. Mediated by a carbodiimide, increasing amounts of thioglycolic acid (TGA) were covalently bound to chitosan (CS) in the first step of modification. In the...
Article
The purpose of this study was to develop thiolated nanoparticles to enhance the bioavailability for the nasal application of leuprolide. Thiolated chitosan-thioglycolic acid (chitosan-TGA) and unmodified chitosan nanoparticles (NPs) were developed via ionic gelation with tripolyphosphate (TPP). Leuprolide was incorporated during the formulation pro...
Article
It was the aim of this study to develop a sustained parenteral peptide (DALCE) delivery system by the immobilization of DALCE to thiolated carboxymethyl dextran-cysteine (CMD-Cys) via disulfide bond formation. The resulting CMD-Cys-DALCE conjugate displayed a 22.6±7.9% (m/m) of DALCE (mean±S.D.; n=3). The conjugation of DALCE with CMD-Cys was confi...
Article
Thiolated chitosans are mucoadhesive polymers that are able to increase the paracellular permeability and improve the oral bioavailability of hydrophilic and macromolecular compounds. Due to the low hydratability of most thiolated chitosans at pH > 6.5, it was the aim of this study to develop a thiomer as a permeation enhancer for mucosal membranes...
Article
Within the present study hydroxyethyl cellulose-cysteamine conjugates are investigated regarding biocompatibility, in situ gelling, permeation enhancing and efflux pump inhibitory properties. For this purpose, a series of concentrations of sodium periodate was prepared to oxidize HEC leading to ring opening of glucose subunits. The resulting polyme...
Article
Nanoparticles generated by complex coacervation of plasmid DNA (pDNA) and modified chitosans namely chitosan-thioglycolic acid (TGA) conjugate and chitosan-HIV-1 Tat peptide conjugate were evaluated as gene delivery systems. In order to optimize transfection efficiency, chitosan-HIV-1 Tat peptide conjugate was combined with chitosan-TGA before its...
Article
The aim of this study was to investigate the potential of poly(acrylic acid)-cysteine (PAA-cys) solution and microparticles to enhance the transport of vitamin B12 (VB 12) across Caco-2 cell monolayer and rat intestinal mucosa. Thiolated PAA was synthesized by covalent attachment of L-cysteine. Microparticles were prepared by spray-drying and chara...
Article
The aim of this study was to examine the potential of self-nanoemulsifying drug delivery systems (SNEDDS) on the uptake of the lipophilic and poorly water soluble phenothiazines thioridazine and chlorpromazine with the isolated plasma derived chylomicron (CM) ex vivo model. The multi-component delivery systems were optimized by evaluating their abi...
Article
This study was aimed at improving the mucoadhesive properties of carboxymethyl dextran by the covalent attachment of cysteine. Mediated by a carbodiimide, l-cysteine was covalently attached to the polymer. The resulting CMD-cysteine conjugate (CMD-(273) conjugate) displayed 273+/-20 micromol thiol groups per gram of polymer (mean+/-S.D.; n=3). With...

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