Deng-Fu Guo- Ph.D
- scientist at University of Iowa
Deng-Fu Guo
- Ph.D
- scientist at University of Iowa
About
184
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Introduction
Research interests focus on obesity induced hypertension. Particularly, understanding molecular mechanisms of obesity, leptin receptor signaling and G protein-coupled receptors involved in the development of obesity.
Current institution
Additional affiliations
March 1991 - December 1997
Education
March 1988 - March 1991
Publications
Publications (184)
Aims
The melanocortin receptor accessory protein 2 (MRAP2), which is abundantly expressed in the brain including the hypothalamus, has emerged as a key regulator of melanocortin-4 receptor (MC4R) activity. We sought to delineate the physiological significance of MRAP2 in MC4R neurons, with a particular focus on autonomic and cardiovascular function...
Obesity and associated conditions such as type 2 diabetes are among the most concerning health issues in the U.S. Leptin receptor (LepR) signaling in the brain plays a pivotal role in the regulation of food intake and energy expenditure. SerpinA3N is a serine protease inhibitor which is highly expressed in the arcuate nucleus of the hypothalamus, u...
Background
The outer mitochondrial Rho GTPase 1, MIRO1, mediates mitochondrial motility within cells, but implications for vascular smooth muscle cell (VSMC) physiology and its roles invascular diseases, such as neointima formation following vascular injury are widely unknown.
Methods
An in vivo model of selective Miro1 deletion in VSMCs was gener...
Obesity is a major public health issue due to its association with type 2 diabetes, hypertension, and other cardiovascular risks. The BBSome, a complex of 8 conserved Bardet-Biedl Syndrome (BBS) proteins, has emerged as a key regulator of energy and glucose homeostasis as well as cardiovascular function. However, the importance of adipocyte BBSome...
Obesity and associated conditions such as type 2 diabetes are among the most concerning issues for health in the U.S. Leptin receptor signaling in the brain plays a pivotal role in the regulation of food intake and energy expenditure. High fat diet induces inflammation within the hypothalamus, leading to leptin insensitivity which contribute to obe...
The autonomic network of the central nervous system (CNS) play an important role in the regulation of many physiological functions. Obesity is often associated with hypertension which is a serious medical condition that increase mortality. Therefore, obesity-induced hypertension may be the consequence of a cardiovascular-metabolic coupling in the C...
Extracellular vesicles (EVs) are lipid-bilayer membrane-enclosed vesicles well known to mediate cell-cell communication to maintain normal physiological functions. The diverse cargo of EVs encompassing proteins, lipids and miRNAs represent potential biomarkers and therapeutic targets. However, the role of adipocyte-derived EVs (AdEVs) in obesity is...
The BBSome, a protein complex of 8 Bardet-Biedl syndrome (BBS) proteins including BBS1, has emerged as an important regulator of metabolic function. BBSome deficient human and mice display several metabolic features including obesity and type 2 diabetes. Here, we show that mice lacking the BBSome specifically in liver which were generated by crossi...
The BBSome, a complex of several Bardet-Biedl syndrome (BBS) proteins including BBS1, has emerged as a critical regulator of energy homeostasis. Although the BBSome is best known for its involvement in cilia trafficking, through a process that involve BBS3, it also regulates the localization of cell membrane receptors underlying metabolic regulatio...
The BBSome is a protein complex composed of eight Bardet-Biedl syndrome (BBS) proteins including BBS1. Humans and mice lacking a functional BBSome display obesity and type 2 diabetes, highlighting the importance of this protein complex for metabolic regulation. However, the contribution of the BBSome in insulin sensitive tissues such as skeletal mu...
The BBSome, a protein complex of 8 Bardet-Biedl syndrome (BBS) proteins including BBS1, has emerged as an important regulator of metabolic, cardiovascular, and autonomic functions. Patients with Bbs gene mutations display severe obesity, type II diabetes and hypertension. Previous studies demonstrated that neuronal BBSome contributes to metabolic h...
Cilia are membrane-bound, microtubular projections emanating from the cell surface and present on virtually all cell types including neurons. Cilia abnormalities (ciliopathies) have been linked to a broad range of diseases including hypertension. Moreover, we previously reported that DOCA-salt hypertension is associated with alterations in cilia in...
In 2020, stay-at-home orders were implemented to stem the spread of SARS-CoV-2 worldwide. Social isolation can be particularly harmful to children and adolescents—during the pandemic, the prevalence of obesity increased by ∼37% in persons aged 2-19. Obesity is often comorbid with type 2 diabetes, which was not assessed in this human pandemic cohort...
The central nervous system (CNS) autonomic network plays an important role in regulation of cardiovascular and metabolic functions. Leptin, a hormone secreted by adipose tissue, acts in the brain to regulate energy homeostasis, cardiovascular function, and several other physiological processes. The melanocortin system including the melanocortin-4 r...
Primary cilia possess an array of receptors and signaling from this organelle is critical for neuronal function. BBS1 is an important component of a receptor trafficking complex (BBSome) that facilitates signaling in both primary cilia and plasma membrane. Humans harboring BBS1 mutations develop obesity and type 2 diabetes. Recent studies from our...
The essential role of mitochondria in physiology and many pathologies has garnered enormous interest in understanding the mechanisms controlling the function of this organelle. The BBSome, a protein complex composed of eight Bardet-Biedl syndrome (BBS) proteins including BBS1, has emerged as an important regulator of various cellular processes. The...
The molecular clock machinery regulates several homeostatic rhythms, including glucose metabolism. We previously demonstrated that Roux-en Y Gastric Bypass (RYGB) has a weight-independent effect on glucose homeostasis, and transiently reduces food intake. In this study we investigate the effects of RYGB on diurnal eating behavior as well as its eff...
Bardet Biedl syndrome (BBS) is a genetic condition associated with various clinical features including cutaneous disorders and certain autoimmune and inflammatory diseases pointing to a potential role of BBS proteins in the regulation of immune function. BBS1 protein which is a key component of the BBSome, a protein complex involved in the regulati...
Objectives
The essential role of mitochondria in regulation of metabolic function and other physiological processes has garnered enormous interest in understanding the mechanisms controlling the function of this organelle. We assessed the role of the BBSome, a protein complex composed of eight Bardet-Biedl syndrome (BBS) proteins, in the control of...
Mitochondria are best known as the powerhouse of the cell playing a critical role in energy metabolism with important implications in the development of obesity, a major cause of type 2 diabetes and hypertension. A-kinase anchoring protein 1 (AKAP1) is a mitochondrial scaffold protein that promote protein kinase A (PKA)-mediated phosphorylation of...
Leptin, a hormone secreted by adipose tissue, is a critical signal influencing energy balance and blood pressure through modulation of the autonomic nervous system. However, the anatomical substrates for leptin regulation of the central autonomic network underlying cardiovascular and metabolic functions remains unknown. Here, we used a retrograde p...
Bardet-Biedl syndrome (BBS) proteins form a protein trafficking complex (BBSome) that regulates the composition of membrane proteins and contributes to neuron function. Patients harboring mutations in any BBS gene results in BBSome dysfunction and the development of metabolic complications including obesity and type 2 diabetes. Studies from our lab...
The BBSome, a protein complex composed of 8 Bardet-Biedl syndrome (BBS) proteins including BBS1 has emerged as critical regulator of energy and glucose homeostasis. Humans and animals bearing disruption of the BBSome through loss of various BBS genes display numerous features including obesity and diabetes, but the underlying mechanisms are not wel...
The BBSome is a protein complex composed of eight Bardet-Biedl syndrome (BBS) proteins including BBS1. Humans and mice lacking a functional BBSome display impaired insulin sensitivity, hyperglycemia, and type 2 diabetes, highlighting the importance of this protein complex in the control of insulin action and glucose homeostasis. However, the contri...
Objective
Bone morphogenetic protein 8B (BMP8B) plays a major role in the regulation of energy homeostasis by modulating brown adipose tissue (BAT) thermogenesis and white adipose tissue (WAT) browning. Here, we investigated whether BMP8B role in metabolism is affected by obesity and the possible molecular mechanisms underlying that action.
Method...
Bardet-Biedl syndrome (BBS) proteins have emerged as critical regulators of various physiological functions including energy and glucose homeostasis, autonomic function and blood pressure. BBS3 protein is a cilia related protein that mediates the ciliary localization of the BBSome, a protein complex composed of eight BBS protein. Consistent with th...
Bardet-Biedl syndrome (BBS) proteins have emerged as critical regulators of various physiological functions including blood pressure. The presence of hypertension in BBS patients is consistent with the finding that BBS knockout mice exhibit increased renal sympathetic nerve activity and arterial pressure. However, the role and contribution of Bbs g...
Objectives
– Endothelial cells that line the entire vascular system play a pivotal role in the control of various physiological processes including metabolism. Moreover, endothelial dysfunction is associated with many pathological conditions including obesity. Here, we assessed the role of the BBSome, a protein complex composed of eight Bardet-Bied...
The mechanistic target of rapamycin complex 1 (mTORC1) signaling complex is emerging as a critical regulator of cardiovascular function with alterations in this pathway implicated in cardiovascular diseases. In this study, we utilized animal models and human tissues to examine the role of vascular mTORC1 signaling in the endothelial dysfunction ass...
Petersen et al. hypothesize that vestibular stress underlies the therapeutic effects of static magnetic and electric fields in type 2 diabetes. However, our experimental data directly contradict the predictions made by this alternative hypothesis. Here, we discuss key findings that support a mechanism that is independent of vestibular stress to exp...
Objectives
The hypothalamic ventromedial nucleus (VMH) is known to play a major role in metabolic control, but the molecular mechanisms involved remains poorly defined. Here, we analyzed the relevance of the BBSome, a protein complex composed of eight Bardet-Biedl syndrome (BBS) proteins including BBS1, in the VMH steroidogenic factor 1 (SF1) neuro...
mTORC1 (Mechanistic target of rapamycin complex 1) serves as a molecular hub and intracellular energy sensor that regulate various cellular processes. Emerging evidence points to mTORC1 signaling as a critical regulator of cardiovascular function with implications for cardiovascular disease. Here, we show that selective disruption of mTORC1, throug...
Graphical Abstract Highlights d RYGB, but not SG, increases energy expenditure (EE) and RMR d This increase in EE is due to sympathetic-mediated ''browning'' of mesenteric fat d CB1 inverse agonist induces splanchnic nerve activity and fat thermogenesis d CB1 agonist attenuates the RYGB-induced weight loss and ''browning'' of mesenteric fat In Brie...
Aberrant redox signaling underlies the pathophysiology of many chronic metabolic diseases, including type 2 diabetes (T2D). Methodologies aimed at rebalancing systemic redox homeostasis have had limited success. A noninvasive, sustained approach would enable the long-term control of redox signaling for the treatment of T2D. We report that static ma...
The BBSome is a protein complex composed of 8 Bardet‐Biedl syndrome (BBS) proteins including BBS1. Patients carrying mutations in any of the BBS genes display several features including obesity and type 2 diabetes mellitus. We previously demonstrated that the BBSome is required for membrane localization of the insulin receptor. We also showed that...
The BBSome, a complex of 8 BBS (Bardet-Biedl syndrome) proteins known for its role in the control of cilia function and other cellular processes, has been implicated in blood pressure control, but the underlying mechanisms are not fully understood. Here, we show that neuronal BBSome plays an important role in blood pressure regulation. Targeted ina...
The BBSome, a protein complex of 8 Bardet-Biedl Syndrome (BBS) protein including BBS1, has emerged as an important regulator of metabolic and cardiovascular function. We previously demonstrated that conditional disruption of the BBSome, by deleting the Bbs1 gene, throughout the nervous system leads to obesity in mice. The ventromedial hypothalamus...
The mechanistic target of rapamycin complex 1 (mTORC1) serves as an intracellular energy sensor through its downstream signaling components the S6-kinase and the ribosomal S6 protein with recent implications for hypertension. In this study, we tested the hypothesis that endothelial mTORC1 signaling is critical for the regulation of vascular endothe...
The BBSome-a complex consisting of 8 Bardet-Biedl syndrome proteins-is involved in the regulation of various cellular processes. Recently, the BBSome complex has emerged as an important regulator of cardiovascular function with implications for disease. In this study, we examined the role of the BBSome in vascular smooth muscle and its effects on t...
Cell motility and migration play critical roles in various physiological processes and disease states. Here, we show that the BBSome, a macromolecule composed of eight Bardet-Biedl syndrome (BBS) proteins including BBS1, is a critical determinant of cell migration and wound healing. Fibroblast cells derived from mice or humans harboring a homozygou...
The BBSome, a complex of eight Bardet-Biedl syndrome (BBS) proteins involved in cilia function, has emerged as an important regulator of energy balance, but the underlying cellular and molecular mechanisms are not fully understood. Here, we show that the control of energy homeostasis by the anorexigenic proopiomelanocortin (POMC) neurons and orexig...
Background
The mechanistic target of rapamycin complex 1 (mTORC1) is an important intracellular energy sensor that regulates gene expression and protein synthesis through its downstream signaling components, the S6‐kinase and the ribosomal S6 protein. Recently, signaling arising from mTORC1 has been implicated in regulation of the cardiovascular sy...
Table S1. qPCR Primer Sequences
Table S2. Aortic Ring EC50 and Emax Values
Figure S1. Valine does not activate mTORC1 signaling in aortic rings ex vivo.
Figure S2. mTOR physically interacts with the p65 subunit of the NFκB transcriptional complex.
RhoBTB1 is an atypical GTPase, which is associated with human hypertension according to a genome‐wide association study. However, the physiological and molecular role of RhoBTB1 is not well understood. Previously, we showed that aortic RhoBTB1 is downregulated in two hypertension models including systemic angiotensin II (Ang II) infusion and vascul...
Bardet‐Biedl syndrome (BBS) is a pleiotropic autosomal recessive human disorder associated with several features including obesity and diabetes mellitus. Mice lacking Bbs genes are also obese and display diabetes as indicated by the increased blood glucose, impaired glucose clearance and insulin resistance. We showed that these changes in glucose a...
The mechanistic target of rapamycin complex 1 (mTORC1) that regulates cellular gene and protein programming through its downstream signaling components the S6‐kinase and the ribosomal S6 protein serves as an intracellular energy sensor. Emerging evidence points to mTORC1 signaling as an important regulator of cardiovascular function with implicatio...
Mice selectively expressing PPARγ dominant negative mutation in vascular smooth muscle exhibit RhoBTB1-deficiency and hypertension. Our rationale was to employ genetic complementation to uncover the mechanism of action of RhoBTB1 in vascular smooth muscle. Inducible smooth muscle-specific restoration of RhoBTB1 fully corrected the hypertension and...
The high prevalence of obesity is associated with increased health risks such as cardiovascular disease and diabetes. A combination of genetic and environmental factors such as availability of nutrition‐rich food contributes to the epidemic of obesity. Recent studies from our group have demonstrated the importance of hypothalamic BBS genes and the...
Obesity‐induced hypertension is associated with vascular endothelial dysfunction. Our laboratory has previously demonstrated a critical role of the mammalian target of rapamycin complex 1 (mTORC1) in the regulation of the cardiovascular system and arterial pressure. mTORC1 serves as an intracellular energy sensor regulating protein synthesis throug...
Peroxisome proliferator‐activated receptor gamma (PPARγ) is a ligand activated transcription factor regulating metabolic and vascular function. We previously reported that mice (S‐DN) expressing dominant‐negative PPARγ in smooth muscle cells (SMC) are hypertensive and exhibit impaired vascular relaxation and enhanced contraction due to increased Rh...
The BBSome is a protein complex composed of 8 Bardet-Biedl syndrome (BBS) proteins, including Bbs1 , involved in the regulation of various cellular processes including trafficking of receptors to cilia and the plasma membrane. This protein complex has emerged as a key regulator of cardiovascular function and blood pressure. We tested the hypothesis...
The brain melanocortin-4 receptor (MC4R) plays an important in the control of food intake and energy expenditure by increasing thermogenic sympathetic nerve activity (SNA). In addition, brain MC4R-mediated activation of SNA subserving cardiovascular organs such as the kidneys increase blood pressure. The melanocortin receptor accessory protein 2 (M...
We previously reported that vascular smooth muscle cell (VSMC) selective expression of hypertension (HT)-causing mutations in either PPARγ or the E3 Ring Ubiquitin Ligase Cullin-3 causes nitric oxide resistance and HT. Here we sought to assess the physiological role of RhoBTB1, a VSMC PPARγ target gene and Cullin-3 substrate adaptor. S-P467L mice w...
The mechanistic target of rapamycin complex 1 (mTORC1) is an important intracellular energy sensor that regulates protein synthesis through its downstream signaling components the S6‐kinase and the ribosomal S6 protein. Recently, our laboratory has demonstrated a critical role of the mTORC1 signaling pathway in the cardiovascular regulation with im...
Objectives:
The autonomic nervous system is critically involved in mediating the control by leptin of many physiological processes. Here, we examined the role of the leptin receptor (LepR) in proopiomelanocortin (POMC) and agouti-related peptide (AgRP) neurons in mediating the effects of leptin on regional sympathetic and parasympathetic nerve act...
The BBSome, a multiplex of 8 Bardet-Biedl Syndrome (BBS) proteins including BBS1, has emerged as an important regulator of energy homeostasis and cardiovascular function. Disrupting the BBSome, through deletion of its units, globally, in the nervous system, hypothalamus or selective neurons such as the proopiomelanocortin (POMC) neurons causes obes...
Obesity is associated with vascular endothelial dysfunction and reactive oxygen species (ROS) signaling. We have previously demonstrated a novel role for the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway in the regulation of cardiovascular reactivity. In this study, we assessed the ability of mTORC1 signaling to generate ROS...
We reported that mice (S-DN) expressing dominant-negative peroxisome proliferator-activated receptor gamma (PPARγ) in smooth muscle cells (SMC) are hypertensive and exhibit impaired vascular relaxation due to increased RhoA/Rho kinase (ROCK) activity, and display reduced expression of a novel PPARγ target gene, RhoBTB1. We hypothesize that RhoBTB1...
Selective expression of dominant negative (DN) peroxisome proliferator-activated receptor γ (PPARγ) in vascular smooth muscle cells (SMC) results in hypertension, atherosclerosis, and increased nuclear factor-κB (NF-κB) target gene expression. Mesenteric SMC were cultured from mice designed to conditionally express wild-type (WT) or DN-PPARγ in res...
The unfolded protein response (UPR), induced by endoplasmic reticulum (ER) stress, regulates the expression of factors that restore protein folding homeostasis. However, in the liver and kidney, ER stress also leads to lipid accumulation, accompanied at least in the liver by transcriptional suppression of metabolic genes. The mechanisms of this acc...
We recently reported that mitoquinone (mitoQ, 500 μmol/L) added to drinking water of C57BL/6J mice attenuated weight gain, decreased food intake, increased hypothalamic orexigenic gene expression, and mitigated oxidative stress when administered from the onset of high-fat (HF) feeding. Here, we examined the effects of mitoQ on pre-existing obesity...
Data S1. Pharmacology, research & perspectives.
Peroxisome proliferator-activated receptor gamma (PPARγ) is a ligand activated transcription factor regulating metabolic and vascular function. We previously reported that mice (S-DN) expressing dominant-negative PPARγ in smooth muscle cells (SMC) are hypertensive, exhibit impaired vascular relaxation and enhanced contraction, and display reduced e...
Bardet-Biedl syndrome (BBS) is a pleiotropic autosomal recessive human disorder associated with several clinical features including obesity and hypertension. We previously demonstrated that Bbs1 gene deletion from the central nervous system caused obesity and hypertension in mice highlighting the importance of neuronal BBS proteins for energy homeo...
Obesity-induced hypertension is associated with vascular endothelial dysfunction. Recently, our laboratory has demonstrated a critical role of the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway in cardiovascular regulation. Here, we tested the hypothesis that dysregulation of mTORC1 signaling is involved in the endothelial dysfu...
PPARγ is a nuclear receptor transcription factor which regulates adipogenesis and energy homeostasis. Recent studies suggest PPARγ may mediate some of its metabolic effects through actions in the brain. We used a Cre-recombinase-dependent (using Nestin(Cre)) conditionally activatable transgene expressing either wild type (WT) or dominant-negative (...
PPARγ, a master regulator of adipogenesis, was recently shown to affect energy homeostasis through its actions in the brain. Deletion of PPARγ in mouse brain, and specifically in the POMC neurons, results in resistance to diet-induced obesity. To study the mechanisms by which PPARγ in POMC neurons controls energy balance, we constructed a Cre-recom...
Peroxisome proliferator‐activated receptor gamma (PPARγ) is a ligand‐activated transcription factor with anti‐inflammatory properties. One of the anti‐inflammatory mechanisms is antagonism of nuclear factor kappa B (NFκB) activity. Synthetic activators of PPARγ are known to inhibit the formation of atherosclerotic lesions in animal models. In contr...
BBS is a pleiotropic autosomal recessive human disorder associated with several clinical features including obesity. We previously demonstrated that Bbs1 gene deletion from the nervous system caused obesity in mice highlighting the importance of neuronal BBS proteins for energy homeostasis. Here, we investigated the role of Bbs1 gene in the orexige...
Bardet‐Biedl Syndrome (BBS) is a cilia‐related disorder characterized by many clinical features including obesity. Global deletion of individual Bbs genes recapitulates many of the phenotypes associated with human BBS including obesity. In addition, our laboratory has demonstrated that conditional deletion of the Bbs1 gene throughout the nervous sy...
Obesity‐induced hypertension is associated with vascular endothelial dysfunction. Recently our laboratory has implicated the mammalian target of rapamycin complex 1 (mTORC1) signaling in the regulation of the cardiovascular system. Specifically, activation of central mTORC1 triggered a significant increase in arterial pressure. Therefore, we tested...
Bardet-Biedl syndrome (BBS) is a highly pleiotropic autosomal recessive disorder associated with a wide range of phenotypes including obesity. However, the underlying mechanism remains unclear. Here, we show that neuronal BBSome is a critical determinant of energy balance through its role in the regulation of the trafficking of the long signaling f...
Relevance of Bbs1 gene in LRb-containing cells for body weight regulation.
(A—B) Average weekly body weights of female (A) and male (B) LRbCre/Bbs1fl/fl mice compared to their littermate controls (n = 11–13 per group). (C—D) Weight of different fat pads of 12- (C) and 25-week old (D) LRbCre/Bbs1fl/fl mice and littermate controls (n = 6 males and 5...
Leptin resistance in LRbcre/Bbs1fl/fl mice.
(A–B) Effect of i.p. administration of leptin (1 μg/g bw, twice a day) on food intake (A) and body weight (B) in obese LRbcre/Bbs1fl/fl mice and control littermates (n = 4 males and 4 females in each group). (C) Comparison of the relative number of LRb positive cells (labeled with td-Tomato) in the rostra...
Postnatal deletion of the Bbs1 gene in the mediobasal hypothalamus increases adiposity.
Weight of various fat pads of female Bbs1fl/fl mice that received microinjection of AAV-Cre or AAV-GFP into the mediobasal hypothalamus at 7–10 weeks of age (n = 7 per group). Data are means ± SEM. *P<0.05 vs. AAV-GFP group.
(TIF)
BBS1 protein interacts with the LRb.
(A) Evidence that the anti-LR antibody (Santa Cruz, sc-8391) recognizes both the LRa and LRb in HEK 293 cells transfected with either the pcDNA3-Flag-LRb (Flag-LRb) or pcDNA3-HA-LRa plasmids. (B) Interaction between the Flag-tagged BBS1 and LRb can be detected (based on co-immunoprecipitation assays) on hypothal...
Mice lacking the Bbs1 gene in the nervous system develop obesity.
(A—B) Average weekly body weights of female (A) and male (B) NestinCre/Bbs1fl/fl mice compared to their littermate controls (n = 6–12 per group). (C) Weight of different fat pads of NestinCre/Bbs1fl/fl mice compared to controls (n = 5 males and 5 females for controls and 5 males and...
(A—B) Average body weights and weight of different fat pads of AdipoCre/Bbs1fl/fl mice and control littermates (n = 2 males and 3 females for controls and 3 males and 3 females for AdipCre/Bbs1fl/fl mice). (C—D) Representative HE staining (C) and quantification of the mean adipocyte size (D) of peri-gonadal fat pad of 12 weeks old Adipocre/Bbs1fl/f...
Validation of FACS to purify brain tdTomato-labelled cells.
(A) Comparison of fluorescence characteristics of dissociated cells from hypothalami of wild type mice and NestinCre/tdTomato mice defines the gating intensities to recognize the tdTomato-positive cells. (B) Sorting of the td-Tomato-positive and -negative hypothalamic cells of LRbCre/tdTom...
Energy imbalance leading to obesity in LRbcre/Bbs1fl/fl mice.
(A—B) Body weights (A) and fat pad weights (B) of LRbCre/Bbs1fl/fl mice pair-fed from 4 to 15 weeks of age relative to age matched controls and LRbCre/Bbs1fl/fl mice fed ad libitum (n = 2 males and 3 females in each group). (C–D) Oxygen consumption (VO2, C) and heat generation (D) of Nes...
Characterization of mice lacking the Ift88 gene in LRb cells.
(A—B) Body weights of female (A) and male (B) LRbcre/Ift88fl/fl mice and littermate controls (n = 11–15 per group). (C—D) Weight of different fat pads of 25 weeks old female (C) and male (D) LRbcre/Ift88fl/fl mice and littermate controls (n = 8–9 per group). (E–F) Effect of i.p. administ...
BBS1 protein regulates LRb trafficking to the cells surface.
(A) Efficacy of BBS1-shRNA in HEK 293 cells to reduce HA-tagged BBS1 protein expression. (B—C) Knockdown of BBS1 attenuate leptin-induced activation of Stat3 (B) and S6K (C) in HEK 293 cells. (D) Efficacy of BBS2-shRNA in HEK 293 cells. (E) Knockdown of BBS2 attenuated leptin-induced acti...
