
Delphine DrouinFrench National Centre for Scientific Research | CNRS · Laboratoire GEPEA - UMR 6144
Delphine Drouin
Chemical Engineer
About
29
Publications
6,677
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
595
Citations
Introduction
Skills and Expertise
Publications
Publications (29)
This study presents the development of a reliable method for quantifying C-phycocyanin. It was found that the spectrophotometric method commonly used for C-phycocyanin quantification tends to overestimate the actual amount of C-phycocyanin in Aphanizomenon flos-aquae (AFA) samples. The aim of this study was therefore to adapt the spectrophotometric...
The current study presents the development of a reliable method for the quantification of c-phycocyanin. It was found that the spectrophotometric method commonly used for c-phycocyanin quantification tends to overestimate the actual amount of c-phycocyanin in AFA samples. Thus, the aim of this study was to account for c-phycocyanin variation betwee...
The SAVANE project (Station Autonome de Valorisation des Algues Naturelles Endogènes) is a winning project of the Bioeconomy and Environment call-for-projects (by the ADEME) to be realized from 2021 to 2024. Includes the participation of SARA and Aglosource societies, and GEPEA laboratory.
The objective of the project is to build a semi-industrial...
The aim of this work is to track and optimize lipid recovery from Nannochloropsis gaditana in wet extraction operations. No significant differences in biomass concentration were found when disrupting microalgal suspensions of up to 30 g/L dry weight, but disruption efficiency differed depending on their physiological states. It took 5.8 min in a be...
The production of lipids by microalgae is widely studied, especially to find the best bioprocess operating conditions and optimize the productivity of the targeted product. In this context, being able to monitor online the evolution of the lipid concentration is a great advantage regarding the control and/or the optimization of the production. Yet,...
The sustainable production of lipids by microalgae is widely developed among the bioprocess community targeting various applications such as feed, food, health or bioenergy. The cultivation of microalgae needs dedicated systems with the optimal illumination geometry. Performing non-invasive online analyses on these bioprocesses is limited to few an...
The Paralytic shellfish neurotoxins (PST) are of increasing interest for biomedical applications. The chemical synthesis is often complex and expensive that's why the purification by membrane filtration of PST from Alexandrium minutum dinoflagellate was investigated. Disrupted micro-alga cells by ultrasonic treatment were diafiltred to let pass tox...
This study takes part of a larger program where the hydrothermal liquefaction (HTL) thermochemical process is used to produce an alternative bitumen from microalgal biomass. The objective is to compare the properties of non-water soluble fractions of two microalgae residues (Scenedesmus sp. and Spirulina) obtained after HTL. The process has been ca...
The hydrothermal liquefaction of Spirulina sp. microalgae (i.e. cyanobacteria) byproducts was investigated for the production of road binders from renewable sources. In the 220-300 °C temperature range, a water-insoluble viscous material was obtained in a ca. 50% yield, which consisted of an oily fatty acid-based fraction mixed with organic and ino...
Samples of nitrogen-starved Parachlorella kessleri containing intact cells (IC), cells ground by bead milling (BM), and cells subjected to high-pressure cell disruption (HPD), together with their supernatants after centrifugation, were compared for granulometry and lipid profiles. The effects of disruption on the lipid profile and organisation were...
We developed an easy-to-use method for the routine analysis of the central metabolism using an affordable low-resolution GC–MS system run in SIM mode. The profiling approach was optimized for the derivatization protocol of some 60 targeted metabolites. The performance of two silylation reagents (MSTFA and BSTFA) that allowed the comprehensive deriv...
Over the past years, the substitution of the classical biochemical quantification techniques by Fourier transform infrared (FTIR) spectroscopy has been widely studied on microalgae because of its tremendous application potential for bioprocess monitoring. In the present work, mandatory aspects that have never been approached by FTIR end-users worki...
A series of rod-shaped and related three-branched push-pull derivatives containing phosphane oxide or phosphane sulfide (PO or PS)-as an electron-withdrawing group conjugated to electron-donating groups, such as amino or ether groups, with a conjugated rod consisting of arylene-vinylene or arylene-ethynylene building blocks-were prepared. These com...
In this paper we present the synthesis, spectroscopic characterization and theoretical modelling of two pairs of correlated dipolar and octupolar donor-acceptor conjugated chromophores, based on the triphenylamine branching centre. The two pairs of chromophores differ for the electron withdrawing end-groups. Linear absorption, fluorescence and two-...
In a previous work, we reported that contrary to native calcium-loaded alpha-lactalbumin (holo alpha-LA), calcium-depleted form (apo alpha-LA) has the ability to self-assemble with lysozyme (LYS) to form different supramolecular structures in temperature-dependent manner. In this study, we examine what happens at molecular scale using fluorescence...
A series of fluorescent phosphane oxide derivatives based on diphenylphosphanoethane (DPPE) and diphenylphosphanomethane (DPPM) skeletons has been prepared by means of Grignard reactions and Sonogashira cross-couplings. The photophysical properties and the linear and nonlinear spectra of these compounds have been investigated. An edge-to-face confo...
Organic nanodots based on the gathering of an exponentially increasing number of two-photon fluorophores on a dendritic platform of controlled size and symmetry represent a promising nontoxic alternative to quantum dots for (bio)imaging purposes. This modular route offers a number of advantages in terms of versatility but also raise a number of que...
The asymmetric synthesis of propargylic fluorides, (+)-6 and (+)-3 with ee’s >95%, is reported. The first key step involves an asymmetric transfer hydrogenation of the propargylic ketone 11, using Noyori’s catalyst, to give alcohol (+)-10. The second important step is the highly stereoselective DAST mediated fluorination of the propargylic alcohol...
The thermal and photochemical ageing of epoxy resin was studied using photoacoustic-FTIR spectroscopy. This technique was satisfactory for both unfilled resin and glass fibre filled epoxy composite. The influence of the curing agent (anhydride or amine) was significant for ageing. The durability of anhydride–epoxy system was the best for both therm...
The changes in the chemical structure and the physical properties of a filled cross-linked polydimethylsiloxane rubber were monitored as a function of various ageing factors. The variables included photochemical, thermal, salt-fog ageings and exposure to acid vapours. Unaged and aged samples were studied by IR spectroscopy, hardness measurements, D...