Dejana Pankovic

• Professor (Full) at Educons University

Advances in informatics, robotics, and imaging techniques make it possible to use state-of-the-art digital reconstruction technologies for high-throughput plant phenotyping (HTPP) affected by stress factors, as well as for the ontology of their structural and functional traits. Digital imaging of structural and functional features of the abovegroun...

Simple Summary Fungi of the genus Trichoderma have a number of positive effects on plants that are manifested in the stimulation of plant growth and tolerance to abiotic and biotic stress factors. Our findings provide evidence for the swift response of tomato seeds to Trichoderma spp. exposure under in vitro conditions. The response was examined af...

The purpose of this study is to examine the effects of different Trichoderma spp. treatments (T1: Trichoderma harzianum, T2: Trichoderma brevicompactum, and T3: T1 + T2) on two tomato cultivars ('Narvik' and 'Gružanski zlatni') by the non-destructive monitoring of chlorophyll (Chl) and epidermal flavonol (Flav) indices and nitrogen balance index (N...

Soil-borne wheat mosaic virus (SBWMV) and Soil-borne cereal mosaic virus (SBCMV), genus Furovirus, family Virgaviridae, cause significant crop losses in cereals. The viruses are transmitted by the soil-borne plasmodiophorid Polymyxa graminis. Inside P. graminis resting spores, the viruses persist in the soil for long time, which makes the disease d...

Cannabis sativa L. known as industrial hemp manifests high capability in the manufacturing of valuable nutraceuticals with health benefits. This study was focused on investigation of chemical profile and bioactive potential of extracts isolated from three hemp varieties (Helena, Marina and Fedora 17). In addition, great attention was directed towar...

Soil pollution with heavy metals is a serious threat to the environment. However, soils polluted with heavy metals are considered good sources of native metal-resistant Trichoderma strains. Trichoderma spp. are free-living fungi commonly isolated from different ecosystems, establishing endophytic associations with plants. They have important ecolog...

The beneficial role of fungi from the Trichoderma genus and its secondary metabolites in promoting plant growth, uptake and use efficiency of macronutrients and oligo/micro-nutrients, activation of plant secondary metabolism and plant protection from diseases makes it interesting for application in environmentally friendly agriculture. However, the...

Resistance to soil-borne viruses as Soil-borne Wheat Mosaic Virus (SBWMV) has gained evident importance in wheat research and breeding. Very few varieties of bread and durum wheat are resistant to these viruses. Search for new sources of SBWMV is conducted worldwide and loci causing resistance have been described on the B and D genomes of tetraploi...

In this study we have investigated the effect of T. harzianum on growth, content of chlorophyll and epidermal flavonols and metal distribution in tomato plants. Trichoderma strain, isolated from the A horizon (5-30 cm) of agricultural soil used in organic production, was applied near the root in the sixth leaf development phase of tomato. Tomato pl...

Trichoderma species are widely known for their antagonistic properties, but plant growth promotion and abiotic stress tolerance have also been reported. Mechanisms of abiotic stress tolerance , such as drought in plants in interaction with Trichoderma spp., remain unclear. In this work, the results about the influence of Trichoderma brevicompactum...

Trichoderma species are widely known for their antagonistic properties, but plant growth promotion and abiotic stress tolerance have also been reported. Mechanisms of abiotic stress tolerance, such as drought in plants in interaction with Trichoderma spp., remain unclear. In this work, the results about the influence of Trichoderma brevicompactum o...

Pre-breeding and elite breeding are two steps in creating high yielding sunflower hybrids that differ in well established procedures and selection methods. However, a methodology that bridge efficient use of introgression lines as product of pre-breeding procedures and their crossing to elite inbreed lines, is not yet very well established. Therefo...

Domestication, selection and modern breeding narrow the genetic variability of barley, and thus create the need for re-integration of variability as a basis for barley breeding. In this study, 4 barley cultivars and 48 landraces from Serbian gene bank were analysed during two years. Phenotypic variability and genetic structure of the two quantitati...

The aim of this study is to assess DNA degradation, DNA amplification, and GMO quantity during tofu production. Soybean seeds were spiked with Roundup Ready® soybeans (RRS) at 0.9, 2, 3 and 5 % (by mass), to assess the level of RSS that would be of practical interest for threshold labelling. Real-time polymerase chain reaction (PCR) was more effect...

Influence of edaphic factors and metal content on diversity of Trichoderma species at 14 different soil sampling locations, on two depths, was examined. Forty-one Trichoderma isolates from 14 sampling sites were determined as nine species based on their internal transcribed spacer (ITS) sequences. Our results indicate that weakly alkaline soils are...

Sunflower line with resistance for downy mildew (Ha-26R) and susceptible line (Ha-26S) were inoculated with the suspension of Plasmopara halstedii zoosporangia, race 730, in the phase of first pair of leaves. The expression of defense related genes was investigated in the time period of 2 to 96 h after treatment. Several categories of defense-relat...

Mullein (Verbascum) plants have extensive distribution and can grow in variable environmental conditions. Seed was collected from mullein plants grown at 4 locations, two metals contaminated and two metal uncontaminated areas. Genetic variability of progeny was examined. Populations collected from unpolluted areas were genetically more similar than...

Two fungal strains were applied to soil polluted with herbicide in order to determine their degradation potential. Three experimental setups were used. In the first setup, the soil in pots was contaminated by linuron in final concentration of 1 ppm. Suspensions of Phanerocheate chrysosporium and Trichoderma asperellum were applied sepa­rately or in...

RÉSUMÉ Le but de cette étude est l’application des marqueurs PCR déjà publiés et le développement des nouveaux dans l’évaluation de résistance du tournesol à la rouille. Vingt lignes cultivées de tournesol ont été examinées. La résistance de plantes à la rouille est déterminée par la méthode d’immersion totale de semailles. Après l’isolation de A.D...

The interaction of photosynthetic electron transport. Calvin cycle and ‘primary’ sink of photosynthetic carbon compounds (cytoplasm) has been investigated in miter stress conditions. Plants of two sunflower hybrids which are differently tolerant to water stress have been grown in semicontrolled conditions in glasshouse. Reduced watering (50–30% of...

Some Trichoderma strains can persist in ecosystems with high concentrations of heavy metals. The aim of this research was to examine the variability of Trichoderma strains isolated from different ecosystems, based on their morphological properties and restriction analysis of ITS fragments. The fungal growth was tested on potato dextrose agar, amend...

The species composition of Trichoderma isolates from the rhizosphere of different vegetables collected at different locations in Hungary was examined during this study. Trichoderma strains were isolated from the rhizosphere samples on dichloran-rose bengal medium. After purification of genomic DNA, the PCR amplification of the internal transcribed...

In this study we screened formerly isolated Trichoderma strains for laccase production on solid media supplemented with two different substrates, ABTS [2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate)] or guaiacol. We detected outstandingly strong colour changes in the case of three Trichoderma strains in this experiment. The strains were identif...

Tebupirimphos or phostebupirim is an organophosphorous type insecticide (OP). A method for the determination of tebupirimphos residues in sugarbeet was developed. The tebupirimphos was extracted from the samples using supercritical fluid extraction (SFE) and analyzed by capillary gas chromatography coupled with nitrogen-phosphorus detection. The LO...

Quinhydrone (QH) is a redox active charge transfer complex commonly used as a redox standard. Information on quinhydrone generation in plants is scarce and its physiological role is still unclear. Recently we have showed that excess zinc may induce oxidative stress through QH accumulation in the cell wall and stabilization of phenoxyl radicals [1]....

The two most important criteria for introducing new confectionary hybrids into production are high seed and protein yield. That is why it is important to find the traits that are measurable, and that at the same time show a strong correlation with seed and protein yield, so that they can be used as a criteria for confectionary hybrid breeding. Resu...

Oxidative stress is one aspect of metal toxicity. Zinc, although unable to perform univalent oxido-reduction reactions, can induce the oxidative damage of cellular components and alter antioxidative systems. Verbascum thapsus L. plants that were grown hydroponically were exposed to 1 and 5 mM Zn²+. Reactive oxygen species (ROS) accumulation was dem...

The objective of this study was to determine the correlation between SSR based genetic distance (GD) of new NS sunflower inbred lines and heterosis for six agronomic traits. Twenty three sunflower inbred lines (20 restorer lines and three female lines used as testers) and their hybrids were examined for plant height, head diameter, thousand seed ma...

The objective of this study was to determine the correlation between SSR based genetic distance (GD) of new NS sunflower inbred lines and heterosis for six agronomic traits. Twenty three sunflower inbred lines (20 restorer lines and three female lines used as testers) and their hybrids were examined for plant height, head diameter, thousand seed ma...

The objective of this study was to determine the relationship between SSR based genetic distance (GD) of new NS sunflower inbred lines for most important agronomic traits and heterosis. Twenty three sunflower inbred lines (twenty restorer lines and three female lines used as testers) were selected based on their diverse genetic background for plant...

In this paper, we have studied the interdependence of seed yield per plant and the following morphophysiological traits: total leaf number per plant, total leaf area per plant, plant height, total seed number per head, head diameter, weight of 1,

The inheritance of resistance to downy mildew of sunflower was investigated in three mapping populations. From each cross 73 to 75 F 3 families were produced. The resistance of plant material to downy mildew was evaluated by the whole seedling inoculation technique. F 3 families were studied using several PCR and two CAPS markers for resistance gen...

The inheritance of the reaction of sunflower to downy mildew was investigated using resistant and susceptible near isogenic lines (NILs) and their F₃ families. Resistance to race 730 was evaluated using the whole seedling inoculation technique. Seventy-three F₃ families were inoculated, among which 54 families were resistant and 19 susceptible, fit...

The inheritance of the reaction of sunflower to downy mildew was investigated using resistant and susceptible near isogenic lines (NILs) and their F 3 families. Resistance to race 730 was evaluated using the whole seedling inoculation technique. Seventy-three F 3 families were inoculated, among which 54 families were resistant and 19 susceptible, f...

The inheritance of the reaction of sunflower to downy mildew was investigated using resistant and susceptible near isogenic lines (NILs) and their F3 families. Resistance to race 730 was evaluated using the whole seedling inoculation technique. Seventy-three F3 families were inoculated, among which 54 families were resistant and 19 susceptible, fit...

The inheritance of the reaction of sunflower to downy mildew was investigated using resistant and susceptible near isogenic lines (NILs) and their F 3 families. Resistance to race 730 was evaluated using the whole seedling inoculation technique. Seventy-three F 3 families were inoculated, among which 54 families were resistant and 19 susceptible, f...

The results of the application of molecular markers in sunflower breeding obtained in the Institute of Field and Vegetable Crops in the last decade are reviewed. Our results on genetic distance (GD=7-75%) between sunflower inbred lines obtained with RAPD and SSR markers, indicate large variability and provide important information for the selection...

The results of the application of molecular markers in sunflower breeding obtained in the Institute of Field and Vegetable Crops in the last decade are reviewed. Our results on genetic distance (GD=7-75%) between sunflower inbred lines obtained with RAPD and SSR markers, indicate large variability and provide important information for the selection...

Phenotype, chromosomes pairing and pollen vitality were compared between parental populations and F1 hybrids of interspecific cross between Helianthus annuus L. and cultivated sunflower. The investigation of the simple sequence repeats (SSR) polymorphism was also used to test the hybrid nature of F1 populations. The phenotypic traits of F1 hybrid p...

Genus Helianthus consists of 49 species. Two species H. giganteus L. and H. maximiliani Sch., distributed and collected in North America, were investigated. In order to determine morphological variability in/between these two species, fifteen populations of each species were used. Thirty traits were measured on five plants per species, grown in the...

A larger proportion of the fixed carbon is retained as starch in the leaf in short days, providing a larger store to support metabolism and carbon export during the long night. The mechanisms that facilitate this adjustment of the sink-source balance are unknown. Starchless pgm mutants were analysed to discover responses that are triggered when diu...

When incorporating desirable traits (resistance to causal agents of various diseases) from the wild relatives into the cultivated sunflower, some undesirable ones are introduced too (branching, small head diameter, low oil content, etc). To overcome this problem, backcrosses (F1 interspecific hybrids x cultivated sunflower) are used, although very...

Increased nitrogen supply stimulates plant growth and photosynthesis. Since it was shown that heavy metals may cause deficiencies of essential nutrients in plants the potential reversal of cadmium toxicity by increased N nutrition was investigated. The effects on photosynthesis of low Cd (0, 0.5, 2 or 5 mmol m−3) combined with three N treatments (2...

The effect of different concentrations of sulfur (1 and 3 mM) and interruption of sulfur (S) supply for 25 days on the photosynthesis and leaf water relations in young sugar beet plants (Beta vulgaris L.) was studied in water culture, under greenhouse conditions. Interruption of S‐supply significantly reduced the content of sulfur, chlorophylls a+b...

The influence of long-term water deficit on photosynthesis, electron transport and carbon metabolism of sunflower leaves has been examined. Water deficit was imposed from flower bud formation up to the stage of full flowering in the field on two sunflower hybrids with different drought tolerance. CO(2) assimilation and stomatal conductance of the i...

The influence of long-term water deficit on photosynthesis, electron transport and carbon metabolism of sunflower leaves has been examined. Water deficit was imposed from flower bud formation up to the stage of full flowering in the field on two sunflower hybrids with different drought tolerance. CO² assimilation and stomatal conductance of the int...

The effect of different lead (Pb) concentrations in the nutrient solution on the growth, Pb and chlorophyll a+b content, chlorophyll fluorescence and quenching parameters in the leaves of young sunflower (Helianthus annuas L.) plants was studied. The content of Pb in the analyzed plant parts increased following the increase in Pb content in the nut...

The effect of varied boron supply (0, 0.5, 1, 25 and 100 μM) in the nutrient solution containing different phosphate concentrations on nutrient utilization, plant growth and photosynthesis was studied in young sunflower (Helianthus annuus L.) plants. Boron deficiency decreased the dry matter yield of plants, leaf area and chlorophyll content in the...

The effect of various boron levels in the nutrient solution on the growth, boron and chlorophyll content, photosynthesis and chlorophyll fluorescence in the leaves of young sunflower (Helianthus annuus L.) plants was studied under greenhouse conditions. Deficiency of boron decreased the dry matter yield of the roots, shoots and leaves. The content...

The effects of phosphate concentration on plant growth and photosynthetic processes in primary leaves of young sunflower (Helianthus annuus L.) plants were examined. Plants were grown for 3 weeks on half-strength Hoagland's solution containing 0, 0.1, 0.5, 1.0, and 3.0 mol m−3 orthophosphate (Pi). It was shown that optimal photosynthesis and the hi...

The genetic diversity and relationships among rapeseed genotypes were evaluated using quantitative analysis and random amplified polymorphic DNA (RAPD) markers. Ten morphological and biochemical traits (plant height, height to the first branch, stem diameter, branch number, leaf number, pod number, seed yield per plant, 1000-seed weight, oil conten...